LOCUS       sequence-327636-        8877 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  For CRISPRi knockdown of ERH by lentiviral delivery of ERH gRNA2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8877)
  AUTHORS   Fang W, Bartel DP
  TITLE     MicroRNA Clustering Assists Processing of Suboptimal MicroRNA 
            Hairpins through the Action of the ERH Protein.
  JOURNAL   Mol Cell. 2020 Apr 16;78(2):289-302.e6. doi: 
            10.1016/j.molcel.2020.01.026.
  PUBMED    32302541
REFERENCE   2  (bases 1 to 8877)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2020.01"; journalName: "Mol Cell"; date: 
            "2020-04-16- 16"; volume: "78"; issue: "2"; pages: "289-302.e6"
COMMENT     Sequence Label: pU6-sgRNA EF1Alpha-puro-T2A-BFP.ERH2
FEATURES             Location/Qualifiers
     source          1..8877
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    519..644
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             691..871
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(889..1087)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(914..934)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        1089..1468
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     1640..1659
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        1734..1838
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1839..1907,1908..2699)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2057..2076)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2870..3458
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3359..3378
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     LTR             3520..3700
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     4209..4225
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     4210..4226
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    4229..4817
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             4335..4346
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     4744..4764
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     protein_bind    4873..4906
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             complement(4926..5624)
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="SELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKVV
                     EGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTAT
                     QDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALKL
                     VGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYCD
                     LPSKLGHKLN"
     CDS             complement(5637..5690)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             complement(5700..6296)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5780..5800)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     6277..6296
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     intron          6317..7255
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     complement(6340..6360)
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     promoter        complement(7256..7467)
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     primer_bind     complement(7712..7732)
                     /label=mU6-F
                     /note="Mouse U6 promoter, forward primer"
     protein_bind    7736..7769
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    8022..8139
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(8437..8481)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    join(8666..8877,1..22)
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
ORIGIN
        1 gaacccaagg aacaaagctc ctattcccac tgctcttttt tctctctgca ccactcttct
       61 ctttgccttg gtgggtgcta ctcctaatgg ttcaattttt actactttat atttatataa
      121 ttcacttctc caattgtccc tcatatctcc tcctccaggt ctgaagatca gcggccggcc
      181 gcttgctgtg cggtggtctt acttttgttt tgctcttcct ctatcttgtc taaagcttcc
      241 ttggtgtctt ttatctctat cctttgatgc acacaataga gggttgctac tgtattatat
      301 aatgatctaa gttcttctga tcctgtctga agggatggtt gtagctgtcc cagtatttgt
      361 ctacagcctt ctgatgtttc taacaggcca ggattaactg cgaatcgttc tagctccctg
      421 cttgcccata ctatatgttt taatttatat tttttctttc cccctggcct taaccgaatt
      481 ttttcccatc gcgatctaat tctcccccgc ttaatactga cgctctcgca cccatctctc
      541 tccttctagc ctccgctagt caaaattttt ggcgtactca ccagtcgccg cccctcgcct
      601 cttgccgtgc gcgcttcagc aagccgagtc ctgcgtcgag agagctcctc tggtttccct
      661 ttcgctttca agtccctgtt cgggcgccac tgctagagat tttccacact gactaaaagg
      721 gtctgaggga tctctagtta ccagagtcac acaacagacg ggcacacact acttgaagca
      781 ctcaaggcaa gctttattga ggcttaagca gtgggttccc tagttagcca gagagctccc
      841 aggctcagat ctggtctaac cagagagacc cagtacaggc aaaacgcgct gcttatatag
      901 acctcccacc gtacacgcct accgcccatt tgcgtcaatg gggcggagtt gttacgacat
      961 tttggaaagt cccgttgatt ttggtgccaa aacaaactcc cattgacgtc aatggggtgg
     1021 agacttggaa atccccgtga gtcaaaccgc tatccacgcc cattgatgta ctgccaaaac
     1081 cgcatcacca tggtaatagc gatgactaat acgtagatgt actgccaagt aggaaagtcc
     1141 cataaggtca tgtactgggc ataatgccag gcgggccatt taccgtcatt gacgtcaata
     1201 gggggcgtac ttggcatatg atacacttga tgtactgcca agtgggcagt ttaccgtaaa
     1261 tactccaccc attgacgtca atggaaagtc cctattggcg ttactatggg aacatacgtc
     1321 attattgacg tcaatgggcg ggggtcgttg ggcggtcagc caggcgggcc atttaccgta
     1381 agttatgtaa cgcggaactc catatatggg ctatgaacta atgaccccgt aattgattac
     1441 tattaataac tagtcaataa tcaatgtcaa cgcgtatatc tggcccgtac atcgcgaagc
     1501 agcgcaaaac gcctaaccct aagcagattc ttcatgcaat tgtcggtcaa gccttgcctt
     1561 gttgtagctt aaattttgct cgcgcactac tcagcgacct ccaacacaca agcagggagc
     1621 agatactggc ttaactatgc ggcatcagag cagattgtac tgagagtgca ccatagggga
     1681 tcgggagatc tcccgatccg tcgacgtcag gtggcacttt tcggggaaat gtgcgcggaa
     1741 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac
     1801 cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg
     1861 tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc
     1921 tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg
     1981 atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga
     2041 gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc
     2101 aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag
     2161 aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga
     2221 gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg
     2281 cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga
     2341 atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt
     2401 tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact
     2461 ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt
     2521 ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg
     2581 ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta
     2641 tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac
     2701 tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta
     2761 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt
     2821 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
     2881 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
     2941 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
     3001 agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
     3061 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
     3121 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
     3181 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
     3241 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
     3301 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
     3361 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
     3421 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
     3481 tacggttcct ggccttttgc tggccttttg ctcacatgct gctagagatt ttccacactg
     3541 actaaaaggg tctgagggat ctctagttac cagagtcaca caacagacgg gcacacacta
     3601 cttgaagcac tcaaggcaag ctttattgag gcttaagcag tgggttccct agttagccag
     3661 agagctccca ggctcagatc tggtctaacc agagagaccc agtacagtcc ggatgcagct
     3721 ctcgggccat gtgatgaaat gctaggcggc tgtcaaacct ccactctaat acttctctct
     3781 ccgggtcatc catcccatgc aggctcacag ggtgtaacaa gcgggtgttc tctccttcat
     3841 tggcttcttc taccttctct tgctcaactg gtactagctt gtagcaccat ccaaaggtca
     3901 gtggatatct gatccctggc cctggtgtgt agttctgcca atcagggaag tagccttgtg
     3961 tgtggtagat ccacagatca aggatatctt gtcttcgttg ggagtgaatt agcccttcca
     4021 gtcccccctt ttcttttaaa aagtggctaa gatctacagc tgccttgtaa gtcattggtc
     4081 ttaaaggtac ctgaggtgtg actggaaaac ccacctcctc ctcctcttgt gcttctagcc
     4141 aggcacaatc agcattggta gctgctgtat tgctacttgt gattgctcca tgtttttcta
     4201 ggtctcgatc gaggtcgacg gtatcgatgc ggggaggcgg cccaaaggga gatccgactc
     4261 gtctgagggc gaaggcgaag acgcggaaga ggccgcagag ccggcagcag gccgcgggaa
     4321 ggaaggtccg ctggattgag ggccgaaggg acgtagcaga aggacgtccc gcgcagaatc
     4381 caggtggcaa cacaggcgag cagccaagga aaggacgatg atttccccga caacaccacg
     4441 gaattgtcag tgcccaacag ccgagcccct gtccagcagc gggcaaggca ggcggcgatg
     4501 agttccgccg tggcaatagg gagggggaaa gcgaaagtcc cggaaaggag ctgacaggtg
     4561 gtggcaatgc cccaaccagt gggggttgcg tcagcaaaca cagtgcacac cacgccacgt
     4621 tgcctgacaa cgggccacaa ctcctcataa agagacagca accaggattt atacaaggag
     4681 gagaaaatga aagccatacg ggaagcaata gcatgataca aaggcattaa agcagcgtat
     4741 ccacatagcg taaaaggagc aacatagtta agaataccag tcaatctttc acaaattttg
     4801 taatccagag gttgattatc gataagcttg atatcgaatt gtacctagtg gaaccggaac
     4861 ccttaaacat gtataacttc gtataatgta tgctatacga agttattagg tccctcgacg
     4921 aattcttaat taagcttgtg ccccagtttg ctagggaggt cgcagtatct ggccactgcc
     4981 acctcgtgct gctcgacgta ggtctcgttg ttggcctcct tgattctttc cagtctgtag
     5041 tccacatagt agacgccagg catcttgagg ttcttagcgg gtttcttgga tctatatgtg
     5101 gtcttgatgt ttgcgatcag atggctcccg cccacgagct tcagggccat gtcgtttctg
     5161 ccttccaggc cgccgtcagc ggggtacagc gtctcggtga aggcctccca gccgagtgtt
     5221 ttcttctgca tcacagggcc gttggatgtg aagttcaccc ctctgatctt gacgttgtag
     5281 atgaggcagc cgtcctggag gctggtgtcc tgggtagcgg tcagcacgcc cccgtcttcg
     5341 tatgtggtga ctctctccca tgtgaagccc tcagggaagg actgcttgaa gaagtcgggg
     5401 atgccctggg tgtggttgat gaaggtcttg ctgccgtaga ggaagctagt agccaggatg
     5461 tcgaaggcga aggggagagg gccgccctcg accaccttga ttctcatggt ctgggtgccc
     5521 tcgtagggct tgccttcgcc ctcggatgtg cacttgaagt gatggttgtc cacggtgccc
     5581 tccatgtaca gcttcatgtg catgttctcc ttaatcagct cgctggtggc gaccggtggg
     5641 ccaggattct cctcgacgtc accgcatgtt agcagacttc ctctgccctc tcccgatccg
     5701 gcaccgggct tgcgggtcat gcaccaggtg cgcggtcctt cgggcacctc gacgtcggcg
     5761 gtgacggtga agccgagccg ctcgtagaag gggaggttgc ggggcgcgga ggtctccagg
     5821 aaggcgggca ccccggcgcg ctcggccgcc tccactccgg ggagcacgac ggcgctgccc
     5881 agacccttgc cctggtggtc gggcgagacg ccgacggtgg ccaggaacca cgcgggctcc
     5941 ttgggccggt gcggcgccag gaggccttcc atctgttgct gcgcggccag ccgggaaccg
     6001 ctcaactcgg ccatgcgcgg gccgatctcg gcgaacaccg cccccgcttc gacgctctcc
     6061 ggcgtggtcc agaccgccac cgcggcgccg tcgtccgcga cccacacctt gccgatgtcg
     6121 agcccgacgc gcgtgaggaa gagttcttgc agctcggtga cccgctcgat gtggcggtcc
     6181 gggtcgacgg tgtggcgcgt ggcggggtag tcggcgaacg cggcggcgag ggtgcgtacg
     6241 gcccggggga cgtcgtcgcg ggtggcgagg cgcaccgtgg gcttgtactc ggtcatggtg
     6301 ggctagctca cgacacctga aatggaagaa aaaaactttg aaccactgtc tgaggcttga
     6361 gaatgaacca agatccaaac tcaaaaaggg caaattccaa ggagaattac atcaagtgcc
     6421 aagctggcct aacttcagtc tccacccact cagtgtgggg aaactccatc gcataaaacc
     6481 cctcccccca acctaaagac gacgtactcc aaaagcgcga gaactaatcg aggtgcctgg
     6541 acggcgcccg gtactccgtg gagtcacatg aagcgacggc tgaggacgga aaggcccttt
     6601 tcctttgtgt gggtgactca cccgcccgct ctcccgagcg ccgcgtcctc cattttgagc
     6661 tccctgcagc agggccggga agcggccatc tttccgctca cgcaactggt gccgaccggg
     6721 ccagccttgc cgcccagggc ggggcgatac acggcggcgc gaggccaggc accagagcag
     6781 gccggccagc ttgagactac ccccgtccga ttctcggtgg ccgcgctcgc aggccccgcc
     6841 tcgccgaaca tgtgcgctgg gacgcacggg ccccgtcgcc gcccgcggcc ccaaaaaccg
     6901 aaataccagt gtgcagatct tggcccgcat ttacaagact atcttgccag aaaaaaagcg
     6961 tcgcagcagg tcatcaaaaa ttttaaatgg ctagagactt atcgaaagca gcgagacagg
     7021 cgcgaaggtg ccaccagatt cgcacgcggc ggccccagcg cccaggccag gcctcaactc
     7081 aagcacgagg cgaaggggct ccttaagcgc aaggcctcga actctcccac ccacttccaa
     7141 cccgaagctc gggatcaaga atcacgtact gcagccaggt ggaagtaatt caaggcacgc
     7201 aagggccata acccgtaaag aggccaggcc cgcgggaacc acacacggca cttacctgtg
     7261 ttctggcggc aaacccgttg cgaaaaagaa cgttcacggc gactactgca cttatatacg
     7321 gttctccccc accctcggga aaaaggcgga gccagtacac gacatcactt tcccagttta
     7381 ccccgcgcca ccttctctag gcaccggttc aattgccgac ccctcccccc aacttctcgg
     7441 ggactgtggg cgatgtgcgc tctgcccact gacgggcaca tgcatggcgg taatacggtt
     7501 atccacgcgg ccgcctaatg gatcctagta ctcgagaaaa aaagcaccga ctcggtgcca
     7561 ctttttcaag ttgataacgg actagcctta tttaaacttg ctatgctgtt tccagcttag
     7621 ctcttaaacc agggacgcca ggcctgaccc aacaaggtgg ttctccaagg gatacttata
     7681 gtctcaaaac acacaattac tttacagtta gggtgagttt ccttttgtgc tgtttataac
     7741 ttcgtataat gtatgctata cgaagttata gaaatccaag cctatcatgt aaaatgtagc
     7801 tagtattaaa aagaacagat tatctgtctt ttatcgcaca ttaagcctct atagttacta
     7861 ggaaatatta tatgcaaatt aaccggggca ggggagtagc cgagcttctc ccacaagtcc
     7921 gtgcgagggg gccggcgcgg gcctagagat ggcgcgtcgg atctctagag cgggcccggt
     7981 actaaccaaa ctggatctct gctgtccctg taataaaccc gaaaattttg aatttttgta
     8041 atttgttttt gtaattcttt agtttgtatg tctgttgcta ttatgtctac tattctttcc
     8101 cctgcactgt accccccaat cccccctttt cttttaaaat tgtggatgaa tactgccatt
     8161 tgtctgcaga attggcgcac gcagtgccga tccgttcact aatcgaatgg atctgtctct
     8221 gtctctctct ccaccttctt cttctattcc ttcgggcctg tcgggtcccc tcggggttgg
     8281 gaggtgggtc tgaaacgata atggtgaata tccctgccta actctattca ctatagaaag
     8341 tacagcaaaa actattctta aacctaccaa gcctcctact atcattatga ataattttat
     8401 ataccacagc caatttgtta tgttaaacca attccacaaa cttgcccatt tatctaattc
     8461 caataattct tgttcattct tttcttgctg gttttgcgat tcttcaatta aggagtgtat
     8521 taagcttgtg taattgttaa tttctctgtc ccactccatc caggtcgtgt gattccaaat
     8581 ctgttccaga gatttattac tccaactagc attccaaggc acagcagtgg tgcaaatgag
     8641 ttttccagag caaccccaaa tccccaggag ctgttgatcc tttaggtatc tttccacagc
     8701 caggattctt gcctggagct gcttgatgcc ccagactgtg agttgcaaca gatgctgttg
     8761 cgcctcaata gccctcagca aattgttctg ctgctgcact ataccagaca ataattgtct
     8821 ggcctgtacc gtcagcgtca ttgacgctgc gcccatagtg cttcctgctg ctcccaa
//
