LOCUS       sequence-327638-        8877 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  For CRISPRi knockdown of ERH by lentiviral delivery of ERH gRNA1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8877)
  AUTHORS   Fang W, Bartel DP
  TITLE     MicroRNA Clustering Assists Processing of Suboptimal MicroRNA 
            Hairpins through the Action of the ERH Protein.
  JOURNAL   Mol Cell. 2020 Apr 16;78(2):289-302.e6. doi: 
            10.1016/j.molcel.2020.01.026.
  PUBMED    32302541
REFERENCE   2  (bases 1 to 8877)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2020.01"; journalName: "Mol Cell"; date: 
            "2020-04-16- 16"; volume: "78"; issue: "2"; pages: "289-302.e6"
COMMENT     Sequence Label: pU6-sgRNA EF1Alpha-puro-T2A-BFP.ERH1
FEATURES             Location/Qualifiers
     source          1..8877
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    530..763
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             948..992
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1290..1407
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    complement(1660..1693)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     1697..1717
                     /label=mU6-F
                     /note="Mouse U6 promoter, forward primer"
     promoter        1962..2173
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     intron          2174..3112
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3069..3089
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             3133..3729
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(3133..3152)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     3629..3649
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     CDS             3739..3792
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             3805..4503
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="SELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKVV
                     EGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTAT
                     QDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALKL
                     VGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYCD
                     LPSKLGHKLN"
     protein_bind    complement(4523..4556)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    4612..5200
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4665..4685)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5083..5094)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(5203..5219)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5204..5220)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             5729..5909
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     rep_origin      complement(5971..6559)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6051..6070)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6730..7521,7522..7590))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7353..7372
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7591..7695)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(7770..7789)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        7961..8340
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        8342..8540
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     8495..8515
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             8558..8738
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    join(8785..8877,1..33)
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
ORIGIN
        1 ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag aattagatcg
       61 cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt aaaacatata
      121 gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt agaaacatca
      181 gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg atcagaagaa
      241 cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag gatagagata
      301 aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag taagaccacc
      361 gcacagcaag cggccggccg ctgatcttca gacctggagg aggagatatg agggacaatt
      421 ggagaagtga attatataaa tataaagtag taaaaattga accattagga gtagcaccca
      481 ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata ggagctttgt
      541 tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg
      601 tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg ctgagggcta
      661 ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag ctccaggcaa
      721 gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt tggggttgct
      781 ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt aataaatctc
      841 tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt aacaattaca
      901 caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag aatgaacaag
      961 aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata acaaattggc
     1021 tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta agaatagttt
     1081 ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta tcgtttcaga
     1141 cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa gaaggtggag
     1201 agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt gcgccaattc
     1261 tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat tggggggtac
     1321 agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa agaattacaa
     1381 aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag agatccagtt
     1441 tggttagtac cgggcccgct ctagagatcc gacgcgccat ctctaggccc gcgccggccc
     1501 cctcgcacgg acttgtggga gaagctcggc tactcccctg ccccggttaa tttgcatata
     1561 atatttccta gtaactatag aggcttaatg tgcgataaaa gacagataat ctgttctttt
     1621 taatactagc tacattttac atgataggct tggatttcta taacttcgta tagcatacat
     1681 tatacgaagt tataaacagc acaaaaggaa actcacccta actgtaaagt aattgtgtgt
     1741 tttgagacta taagtatccc ttggagaacc accttgttgg cgtgtaacgg cggcggtgtg
     1801 tttaagagct aagctggaaa cagcatagca agtttaaata aggctagtcc gttatcaact
     1861 tgaaaaagtg gcaccgagtc ggtgcttttt ttctcgagta ctaggatcca ttaggcggcc
     1921 gcgtggataa ccgtattacc gccatgcatg tgcccgtcag tgggcagagc gcacatcgcc
     1981 cacagtcccc gagaagttgg ggggaggggt cggcaattga accggtgcct agagaaggtg
     2041 gcgcggggta aactgggaaa gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg
     2101 gggagaaccg tatataagtg cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc
     2161 cgccagaaca caggtaagtg ccgtgtgtgg ttcccgcggg cctggcctct ttacgggtta
     2221 tggcccttgc gtgccttgaa ttacttccac ctggctgcag tacgtgattc ttgatcccga
     2281 gcttcgggtt ggaagtgggt gggagagttc gaggccttgc gcttaaggag ccccttcgcc
     2341 tcgtgcttga gttgaggcct ggcctgggcg ctggggccgc cgcgtgcgaa tctggtggca
     2401 ccttcgcgcc tgtctcgctg ctttcgataa gtctctagcc atttaaaatt tttgatgacc
     2461 tgctgcgacg ctttttttct ggcaagatag tcttgtaaat gcgggccaag atctgcacac
     2521 tggtatttcg gtttttgggg ccgcgggcgg cgacggggcc cgtgcgtccc agcgcacatg
     2581 ttcggcgagg cggggcctgc gagcgcggcc accgagaatc ggacgggggt agtctcaagc
     2641 tggccggcct gctctggtgc ctggcctcgc gccgccgtgt atcgccccgc cctgggcggc
     2701 aaggctggcc cggtcggcac cagttgcgtg agcggaaaga tggccgcttc ccggccctgc
     2761 tgcagggagc tcaaaatgga ggacgcggcg ctcgggagag cgggcgggtg agtcacccac
     2821 acaaaggaaa agggcctttc cgtcctcagc cgtcgcttca tgtgactcca cggagtaccg
     2881 ggcgccgtcc aggcacctcg attagttctc gcgcttttgg agtacgtcgt ctttaggttg
     2941 gggggagggg ttttatgcga tggagtttcc ccacactgag tgggtggaga ctgaagttag
     3001 gccagcttgg cacttgatgt aattctcctt ggaatttgcc ctttttgagt ttggatcttg
     3061 gttcattctc aagcctcaga cagtggttca aagttttttt cttccatttc aggtgtcgtg
     3121 agctagccca ccatgaccga gtacaagccc acggtgcgcc tcgccacccg cgacgacgtc
     3181 ccccgggccg tacgcaccct cgccgccgcg ttcgccgact accccgccac gcgccacacc
     3241 gtcgacccgg accgccacat cgagcgggtc accgagctgc aagaactctt cctcacgcgc
     3301 gtcgggctcg acatcggcaa ggtgtgggtc gcggacgacg gcgccgcggt ggcggtctgg
     3361 accacgccgg agagcgtcga agcgggggcg gtgttcgccg agatcggccc gcgcatggcc
     3421 gagttgagcg gttcccggct ggccgcgcag caacagatgg aaggcctcct ggcgccgcac
     3481 cggcccaagg agcccgcgtg gttcctggcc accgtcggcg tctcgcccga ccaccagggc
     3541 aagggtctgg gcagcgccgt cgtgctcccc ggagtggagg cggccgagcg cgccggggtg
     3601 cccgccttcc tggagacctc cgcgccccgc aacctcccct tctacgagcg gctcggcttc
     3661 accgtcaccg ccgacgtcga ggtgcccgaa ggaccgcgca cctggtgcat gacccgcaag
     3721 cccggtgccg gatcgggaga gggcagagga agtctgctaa catgcggtga cgtcgaggag
     3781 aatcctggcc caccggtcgc caccagcgag ctgattaagg agaacatgca catgaagctg
     3841 tacatggagg gcaccgtgga caaccatcac ttcaagtgca catccgaggg cgaaggcaag
     3901 ccctacgagg gcacccagac catgagaatc aaggtggtcg agggcggccc tctccccttc
     3961 gccttcgaca tcctggctac tagcttcctc tacggcagca agaccttcat caaccacacc
     4021 cagggcatcc ccgacttctt caagcagtcc ttccctgagg gcttcacatg ggagagagtc
     4081 accacatacg aagacggggg cgtgctgacc gctacccagg acaccagcct ccaggacggc
     4141 tgcctcatct acaacgtcaa gatcagaggg gtgaacttca catccaacgg ccctgtgatg
     4201 cagaagaaaa cactcggctg ggaggccttc accgagacgc tgtaccccgc tgacggcggc
     4261 ctggaaggca gaaacgacat ggccctgaag ctcgtgggcg ggagccatct gatcgcaaac
     4321 atcaagacca catatagatc caagaaaccc gctaagaacc tcaagatgcc tggcgtctac
     4381 tatgtggact acagactgga aagaatcaag gaggccaaca acgagaccta cgtcgagcag
     4441 cacgaggtgg cagtggccag atactgcgac ctccctagca aactggggca caagcttaat
     4501 taagaattcg tcgagggacc taataacttc gtatagcata cattatacga agttatacat
     4561 gtttaagggt tccggttcca ctaggtacaa ttcgatatca agcttatcga taatcaacct
     4621 ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg
     4681 ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc
     4741 attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt
     4801 gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc
     4861 attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg
     4921 gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact
     4981 gacaattccg tggtgttgtc ggggaaatca tcgtcctttc cttggctgct cgcctgtgtt
     5041 gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg
     5101 gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc
     5161 cctcagacga gtcggatctc cctttgggcc gcctccccgc atcgataccg tcgacctcga
     5221 tcgagaccta gaaaaacatg gagcaatcac aagtagcaat acagcagcta ccaatgctga
     5281 ttgtgcctgg ctagaagcac aagaggagga ggaggtgggt tttccagtca cacctcaggt
     5341 acctttaaga ccaatgactt acaaggcagc tgtagatctt agccactttt taaaagaaaa
     5401 ggggggactg gaagggctaa ttcactccca acgaagacaa gatatccttg atctgtggat
     5461 ctaccacaca caaggctact tccctgattg gcagaactac acaccagggc cagggatcag
     5521 atatccactg acctttggat ggtgctacaa gctagtacca gttgagcaag agaaggtaga
     5581 agaagccaat gaaggagaga acacccgctt gttacaccct gtgagcctgc atgggatgga
     5641 tgacccggag agagaagtat tagagtggag gtttgacagc cgcctagcat ttcatcacat
     5701 ggcccgagag ctgcatccgg actgtactgg gtctctctgg ttagaccaga tctgagcctg
     5761 ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt
     5821 gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc
     5881 cttttagtca gtgtggaaaa tctctagcag catgtgagca aaaggccagc aaaaggccag
     5941 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
     6001 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
     6061 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
     6121 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
     6181 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
     6241 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
     6301 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
     6361 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt
     6421 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
     6481 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
     6541 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
     6601 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
     6661 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
     6721 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
     6781 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
     6841 atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc
     6901 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
     6961 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
     7021 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat
     7081 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
     7141 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
     7201 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
     7261 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
     7321 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt
     7381 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct
     7441 gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac
     7501 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
     7561 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat
     7621 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
     7681 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtcgacg gatcgggaga
     7741 tctcccgatc ccctatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc
     7801 cagtatctgc tccctgcttg tgtgttggag gtcgctgagt agtgcgcgag caaaatttaa
     7861 gctacaacaa ggcaaggctt gaccgacaat tgcatgaaga atctgcttag ggttaggcgt
     7921 tttgcgctgc ttcgcgatgt acgggccaga tatacgcgtt gacattgatt attgactagt
     7981 tattaatagt aatcaattac ggggtcatta gttcatagcc catatatgga gttccgcgtt
     8041 acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg cccattgacg
     8101 tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg acgtcaatgg
     8161 gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca tatgccaagt
     8221 acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc ccagtacatg
     8281 accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc tattaccatg
     8341 gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt
     8401 ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac
     8461 tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg
     8521 tgggaggtct atataagcag cgcgttttgc ctgtactggg tctctctggt tagaccagat
     8581 ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt
     8641 gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc
     8701 cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa cagggacttg
     8761 aaagcgaaag ggaaaccaga ggagctctct cgacgcagga ctcggcttgc tgaagcgcgc
     8821 acggcaagag gcgaggggcg gcgactggtg agtacgccaa aaattttgac tagcgga
//
