LOCUS       sequence-327639-        9128 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express miR-144~451 under the U6 promoter in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9128)
  AUTHORS   Fang W, Bartel DP
  TITLE     MicroRNA Clustering Assists Processing of Suboptimal MicroRNA 
            Hairpins through the Action of the ERH Protein.
  JOURNAL   Mol Cell. 2020 Apr 16;78(2):289-302.e6. doi: 
            10.1016/j.molcel.2020.01.026.
  PUBMED    32302541
REFERENCE   2  (bases 1 to 9128)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2020.01"; journalName: "Mol Cell"; date: 
            "2020-04-16- 16"; volume: "78"; issue: "2"; pages: "289-302.e6"
COMMENT     Sequence Label: pU6-mir144~451 EF1Alpha-puro-T2A-BFP
FEATURES             Location/Qualifiers
     source          1..9128
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    292..417
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             464..644
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(662..860)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(687..707)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        862..1241
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     1413..1432
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        1507..1611
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1612..1680,1681..2472)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1830..1849)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2643..3231
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3132..3151
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     LTR             3293..3473
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     3982..3998
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     3983..3999
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    4002..4590
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             4108..4119
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     4517..4537
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     protein_bind    4646..4679
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             complement(4699..5397)
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="SELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKVV
                     EGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTAT
                     QDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALKL
                     VGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYCD
                     LPSKLGHKLN"
     CDS             complement(5410..5463)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             complement(5473..6069)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5553..5573)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     6050..6069
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     intron          6090..7028
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     complement(6113..6133)
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     promoter        complement(7029..7240)
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     primer_bind     complement(7736..7756)
                     /label=mU6-F
                     /note="Mouse U6 promoter, forward primer"
     protein_bind    7760..7793
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    8046..8163
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(8461..8505)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    8690..8923
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
ORIGIN
        1 gtctaaagct tccttggtgt cttttatctc tatcctttga tgcacacaat agagggttgc
       61 tactgtatta tataatgatc taagttcttc tgatcctgtc tgaagggatg gttgtagctg
      121 tcccagtatt tgtctacagc cttctgatgt ttctaacagg ccaggattaa ctgcgaatcg
      181 ttctagctcc ctgcttgccc atactatatg ttttaattta tattttttct ttccccctgg
      241 ccttaaccga attttttccc atcgcgatct aattctcccc cgcttaatac tgacgctctc
      301 gcacccatct ctctccttct agcctccgct agtcaaaatt tttggcgtac tcaccagtcg
      361 ccgcccctcg cctcttgccg tgcgcgcttc agcaagccga gtcctgcgtc gagagagctc
      421 ctctggtttc cctttcgctt tcaagtccct gttcgggcgc cactgctaga gattttccac
      481 actgactaaa agggtctgag ggatctctag ttaccagagt cacacaacag acgggcacac
      541 actacttgaa gcactcaagg caagctttat tgaggcttaa gcagtgggtt ccctagttag
      601 ccagagagct cccaggctca gatctggtct aaccagagag acccagtaca ggcaaaacgc
      661 gctgcttata tagacctccc accgtacacg cctaccgccc atttgcgtca atggggcgga
      721 gttgttacga cattttggaa agtcccgttg attttggtgc caaaacaaac tcccattgac
      781 gtcaatgggg tggagacttg gaaatccccg tgagtcaaac cgctatccac gcccattgat
      841 gtactgccaa aaccgcatca ccatggtaat agcgatgact aatacgtaga tgtactgcca
      901 agtaggaaag tcccataagg tcatgtactg ggcataatgc caggcgggcc atttaccgtc
      961 attgacgtca atagggggcg tacttggcat atgatacact tgatgtactg ccaagtgggc
     1021 agtttaccgt aaatactcca cccattgacg tcaatggaaa gtccctattg gcgttactat
     1081 gggaacatac gtcattattg acgtcaatgg gcgggggtcg ttgggcggtc agccaggcgg
     1141 gccatttacc gtaagttatg taacgcggaa ctccatatat gggctatgaa ctaatgaccc
     1201 cgtaattgat tactattaat aactagtcaa taatcaatgt caacgcgtat atctggcccg
     1261 tacatcgcga agcagcgcaa aacgcctaac cctaagcaga ttcttcatgc aattgtcggt
     1321 caagccttgc cttgttgtag cttaaatttt gctcgcgcac tactcagcga cctccaacac
     1381 acaagcaggg agcagatact ggcttaacta tgcggcatca gagcagattg tactgagagt
     1441 gcaccatagg ggatcgggag atctcccgat ccgtcgacgt caggtggcac ttttcgggga
     1501 aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc
     1561 atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt
     1621 caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct
     1681 cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt
     1741 tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt
     1801 tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac
     1861 gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac
     1921 tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct
     1981 gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg
     2041 aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg
     2101 gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca
     2161 atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa
     2221 caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt
     2281 ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc
     2341 attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg
     2401 agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt
     2461 aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt
     2521 catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc
     2581 ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct
     2641 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta
     2701 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc
     2761 ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac
     2821 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct
     2881 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat
     2941 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg
     3001 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa
     3061 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
     3121 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
     3181 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc
     3241 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gctgctagag
     3301 attttccaca ctgactaaaa gggtctgagg gatctctagt taccagagtc acacaacaga
     3361 cgggcacaca ctacttgaag cactcaaggc aagctttatt gaggcttaag cagtgggttc
     3421 cctagttagc cagagagctc ccaggctcag atctggtcta accagagaga cccagtacag
     3481 tccggatgca gctctcgggc catgtgatga aatgctaggc ggctgtcaaa cctccactct
     3541 aatacttctc tctccgggtc atccatccca tgcaggctca cagggtgtaa caagcgggtg
     3601 ttctctcctt cattggcttc ttctaccttc tcttgctcaa ctggtactag cttgtagcac
     3661 catccaaagg tcagtggata tctgatccct ggccctggtg tgtagttctg ccaatcaggg
     3721 aagtagcctt gtgtgtggta gatccacaga tcaaggatat cttgtcttcg ttgggagtga
     3781 attagccctt ccagtccccc cttttctttt aaaaagtggc taagatctac agctgccttg
     3841 taagtcattg gtcttaaagg tacctgaggt gtgactggaa aacccacctc ctcctcctct
     3901 tgtgcttcta gccaggcaca atcagcattg gtagctgctg tattgctact tgtgattgct
     3961 ccatgttttt ctaggtctcg atcgaggtcg acggtatcga tgcggggagg cggcccaaag
     4021 ggagatccga ctcgtctgag ggcgaaggcg aagacgcgga agaggccgca gagccggcag
     4081 caggccgcgg gaaggaaggt ccgctggatt gagggccgaa gggacgtagc agaaggacgt
     4141 cccgcgcaga atccaggtgg caacacaggc gagcagccaa ggaaaggacg atgatttccc
     4201 cgacaacacc acggaattgt cagtgcccaa cagccgagcc cctgtccagc agcgggcaag
     4261 gcaggcggcg atgagttccg ccgtggcaat agggaggggg aaagcgaaag tcccggaaag
     4321 gagctgacag gtggtggcaa tgccccaacc agtgggggtt gcgtcagcaa acacagtgca
     4381 caccacgcca cgttgcctga caacgggcca caactcctca taaagagaca gcaaccagga
     4441 tttatacaag gaggagaaaa tgaaagccat acgggaagca atagcatgat acaaaggcat
     4501 taaagcagcg tatccacata gcgtaaaagg agcaacatag ttaagaatac cagtcaatct
     4561 ttcacaaatt ttgtaatcca gaggttgatt atcgataagc ttgatatcga attgtaccta
     4621 gtggaaccgg aacccttaaa catgtataac ttcgtataat gtatgctata cgaagttatt
     4681 aggtccctcg acgaattctt aattaagctt gtgccccagt ttgctaggga ggtcgcagta
     4741 tctggccact gccacctcgt gctgctcgac gtaggtctcg ttgttggcct ccttgattct
     4801 ttccagtctg tagtccacat agtagacgcc aggcatcttg aggttcttag cgggtttctt
     4861 ggatctatat gtggtcttga tgtttgcgat cagatggctc ccgcccacga gcttcagggc
     4921 catgtcgttt ctgccttcca ggccgccgtc agcggggtac agcgtctcgg tgaaggcctc
     4981 ccagccgagt gttttcttct gcatcacagg gccgttggat gtgaagttca cccctctgat
     5041 cttgacgttg tagatgaggc agccgtcctg gaggctggtg tcctgggtag cggtcagcac
     5101 gcccccgtct tcgtatgtgg tgactctctc ccatgtgaag ccctcaggga aggactgctt
     5161 gaagaagtcg gggatgccct gggtgtggtt gatgaaggtc ttgctgccgt agaggaagct
     5221 agtagccagg atgtcgaagg cgaaggggag agggccgccc tcgaccacct tgattctcat
     5281 ggtctgggtg ccctcgtagg gcttgccttc gccctcggat gtgcacttga agtgatggtt
     5341 gtccacggtg ccctccatgt acagcttcat gtgcatgttc tccttaatca gctcgctggt
     5401 ggcgaccggt gggccaggat tctcctcgac gtcaccgcat gttagcagac ttcctctgcc
     5461 ctctcccgat ccggcaccgg gcttgcgggt catgcaccag gtgcgcggtc cttcgggcac
     5521 ctcgacgtcg gcggtgacgg tgaagccgag ccgctcgtag aaggggaggt tgcggggcgc
     5581 ggaggtctcc aggaaggcgg gcaccccggc gcgctcggcc gcctccactc cggggagcac
     5641 gacggcgctg cccagaccct tgccctggtg gtcgggcgag acgccgacgg tggccaggaa
     5701 ccacgcgggc tccttgggcc ggtgcggcgc caggaggcct tccatctgtt gctgcgcggc
     5761 cagccgggaa ccgctcaact cggccatgcg cgggccgatc tcggcgaaca ccgcccccgc
     5821 ttcgacgctc tccggcgtgg tccagaccgc caccgcggcg ccgtcgtccg cgacccacac
     5881 cttgccgatg tcgagcccga cgcgcgtgag gaagagttct tgcagctcgg tgacccgctc
     5941 gatgtggcgg tccgggtcga cggtgtggcg cgtggcgggg tagtcggcga acgcggcggc
     6001 gagggtgcgt acggcccggg ggacgtcgtc gcgggtggcg aggcgcaccg tgggcttgta
     6061 ctcggtcatg gtgggctagc tcacgacacc tgaaatggaa gaaaaaaact ttgaaccact
     6121 gtctgaggct tgagaatgaa ccaagatcca aactcaaaaa gggcaaattc caaggagaat
     6181 tacatcaagt gccaagctgg cctaacttca gtctccaccc actcagtgtg gggaaactcc
     6241 atcgcataaa acccctcccc ccaacctaaa gacgacgtac tccaaaagcg cgagaactaa
     6301 tcgaggtgcc tggacggcgc ccggtactcc gtggagtcac atgaagcgac ggctgaggac
     6361 ggaaaggccc ttttcctttg tgtgggtgac tcacccgccc gctctcccga gcgccgcgtc
     6421 ctccattttg agctccctgc agcagggccg ggaagcggcc atctttccgc tcacgcaact
     6481 ggtgccgacc gggccagcct tgccgcccag ggcggggcga tacacggcgg cgcgaggcca
     6541 ggcaccagag caggccggcc agcttgagac tacccccgtc cgattctcgg tggccgcgct
     6601 cgcaggcccc gcctcgccga acatgtgcgc tgggacgcac gggccccgtc gccgcccgcg
     6661 gccccaaaaa ccgaaatacc agtgtgcaga tcttggcccg catttacaag actatcttgc
     6721 cagaaaaaaa gcgtcgcagc aggtcatcaa aaattttaaa tggctagaga cttatcgaaa
     6781 gcagcgagac aggcgcgaag gtgccaccag attcgcacgc ggcggcccca gcgcccaggc
     6841 caggcctcaa ctcaagcacg aggcgaaggg gctccttaag cgcaaggcct cgaactctcc
     6901 cacccacttc caacccgaag ctcgggatca agaatcacgt actgcagcca ggtggaagta
     6961 attcaaggca cgcaagggcc ataacccgta aagaggccag gcccgcggga accacacacg
     7021 gcacttacct gtgttctggc ggcaaacccg ttgcgaaaaa gaacgttcac ggcgactact
     7081 gcacttatat acggttctcc cccaccctcg ggaaaaaggc ggagccagta cacgacatca
     7141 ctttcccagt ttaccccgcg ccaccttctc taggcaccgg ttcaattgcc gacccctccc
     7201 cccaacttct cggggactgt gggcgatgtg cgctctgccc actgacgggc acatgcatgg
     7261 cggtaatacg gttatccacg cggccgccta atggatccta gtactcgagt ggagtctccc
     7321 ttccagtagt ctgcttcagg gtcctgagtt ctcttcctgg cacgttttct gggtatagca
     7381 agagaaccat taccattact aaactcagta atggtaacgg tttccttgcc attcccaagt
     7441 gccctggcag tcagtaggtt gtgacaggct gagcagagag cttcttgggc ttgcagcatc
     7501 tctcctgtcc tccttgtcag gctccagagc tgggggtgcc cggactagta catcatctat
     7561 actgtagtgt ctcatcgcaa acttacagta tatgatgata tcccagccag ggccccactg
     7621 ggggcacagg aagcatggct tgaaaagcac tgtgaggggc tgggggcaga acccaacaag
     7681 gtggttctcc aagggatact tatagtctca aaacacacaa ttactttaca gttagggtga
     7741 gtttcctttt gtgctgttta taacttcgta taatgtatgc tatacgaagt tatagaaatc
     7801 caagcctatc atgtaaaatg tagctagtat taaaaagaac agattatctg tcttttatcg
     7861 cacattaagc ctctatagtt actaggaaat attatatgca aattaaccgg ggcaggggag
     7921 tagccgagct tctcccacaa gtccgtgcga gggggccggc gcgggcctag agatggcgcg
     7981 tcggatctct agagcgggcc cggtactaac caaactggat ctctgctgtc cctgtaataa
     8041 acccgaaaat tttgaatttt tgtaatttgt ttttgtaatt ctttagtttg tatgtctgtt
     8101 gctattatgt ctactattct ttcccctgca ctgtaccccc caatcccccc ttttctttta
     8161 aaattgtgga tgaatactgc catttgtctg cagaattggc gcacgcagtg ccgatccgtt
     8221 cactaatcga atggatctgt ctctgtctct ctctccacct tcttcttcta ttccttcggg
     8281 cctgtcgggt cccctcgggg ttgggaggtg ggtctgaaac gataatggtg aatatccctg
     8341 cctaactcta ttcactatag aaagtacagc aaaaactatt cttaaaccta ccaagcctcc
     8401 tactatcatt atgaataatt ttatatacca cagccaattt gttatgttaa accaattcca
     8461 caaacttgcc catttatcta attccaataa ttcttgttca ttcttttctt gctggttttg
     8521 cgattcttca attaaggagt gtattaagct tgtgtaattg ttaatttctc tgtcccactc
     8581 catccaggtc gtgtgattcc aaatctgttc cagagattta ttactccaac tagcattcca
     8641 aggcacagca gtggtgcaaa tgagttttcc agagcaaccc caaatcccca ggagctgttg
     8701 atcctttagg tatctttcca cagccaggat tcttgcctgg agctgcttga tgccccagac
     8761 tgtgagttgc aacagatgct gttgcgcctc aatagccctc agcaaattgt tctgctgctg
     8821 cactatacca gacaataatt gtctggcctg taccgtcagc gtcattgacg ctgcgcccat
     8881 agtgcttcct gctgctccca agaacccaag gaacaaagct cctattccca ctgctctttt
     8941 ttctctctgc accactcttc tctttgcctt ggtgggtgct actcctaatg gttcaatttt
     9001 tactacttta tatttatata attcacttct ccaattgtcc ctcatatctc ctcctccagg
     9061 tctgaagatc agcggccggc cgcttgctgt gcggtggtct tacttttgtt ttgctcttcc
     9121 tctatctt
//
