LOCUS       sequence-327643-         507 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses Cre recombinase and an sgRNA targeting Zmat3.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 507)
  AUTHORS   Bieging-Rolett KT, Kaiser AM, Morgens DW, Boutelle AM, Seoane JA, 
            Van Nostrand EL, Zhu C, Houlihan SL, Mello SS, Yee BA, McClendon J, 
            Pierce SE, Winters IP, Wang M, Connolly AJ, Lowe SW, Curtis C, Yeo 
            GW, Winslow MM, Bassik MC, Attardi LD
  TITLE     Zmat3 Is a Key Splicing Regulator in the p53 Tumor Suppression 
            Program.
  JOURNAL   Mol Cell. 2020 Nov 5;80(3):452-469.e9. doi: 
            10.1016/j.molcel.2020.10.022.
  PUBMED    33157015
REFERENCE   2  (bases 1 to 507)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2020.10"; journalName: "Mol Cell"; date: 
            "2020-11-5- 5"; volume: "80"; issue: "3"; pages: "452-469.e9"
COMMENT     Sequence Label: Lenti-sgZmat3.55.9/Cre
FEATURES             Location/Qualifiers
     source          1..507
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     23..42
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        119..194
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     primer_bind     complement(282..305)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
ORIGIN
        1 tttaaaatta tgttttaaaa tggactatca tatgcttacc gtaacttgaa agtatttcga
       61 tttcttggct ttatatatct tgtggaaagg accaaacacc gctcccctgc cgtggcacgt
      121 tttagagcta gaaatagcaa gttaaaataa ggctagtccg ttatcaactt gaaaaagtgg
      181 caccgagtcg gtgctttttt gtttggcgcg cctatggctt aattaacatg gcggccgcat
      241 tctaccgggt aggggaggcg cttttcccaa ggcagtctgg agcatgcgct ttagcagccc
      301 cgctgggcac ttggcgctac acaagtggcc tctggcctcg cacacattcc acatccaccg
      361 gtaggcgcca accggctccg ttctttggtg gccccttcgc gccaccttct actcctcccc
      421 tagtcaggaa gttccccccc gccccgcagc tcgcgtcgtg caggacgtga caaatggaag
      481 tagcacgtct cactagtctc gtgcaga
//
