LOCUS       sequence-327660-       11174 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  mammalian expression of SARS-CoV soluble spike trimer protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11174)
  AUTHORS   Esposito D, Mehalko J, Drew M, Snead K, Wall V, Taylor T, Frank P, 
            Denson JP, Hong M, Gulten G, Sadtler K, Messing S, Gillette W
  TITLE     Optimizing high-yield production of SARS-CoV-2 soluble spike trimers
            for serology assays.
  JOURNAL   Protein Expr Purif. 2020 Jun 4;174:105686. doi: 
            10.1016/j.pep.2020.105686.
  PUBMED    32504802
REFERENCE   2  (bases 1 to 11174)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Protein 
            Expr Purif."; date: "2020-06-4"; volume: "174:105686. doi"; pages: "
            10.1016/j.pep.2020.105686"
COMMENT     Sequence Label: R619-M87-303: CMV51p> SARS-CoV
            S-2P-T4f-3C-His...
FEATURES             Location/Qualifiers
     source          1..11174
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      204..1993
                     /label=oriP
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
     primer_bind     complement(2030..2049)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     protein_bind    2199..2219
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     enhancer        2230..2533
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2534..2737
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2688..2708
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2734..2758
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    2803..2827
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             6496..6519
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             6523..6546
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     CDS             6553..6576
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             6616..6639
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     protein_bind    complement(6641..6665)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        complement(6727..6745)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(6728..6745)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(6765..6782)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6771..6995
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      7041..7469
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7128..7147)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7338..7359
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7478..7498)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7483..7812
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7663..7798
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             7879..8673
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7933..7952)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     8543..8562
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    8847..8968
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8884..8903)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8938..8957
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(9017..9033)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9017..9033)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9030..9052)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9041..9057
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9065..9071,9072..9089,9090..9095))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9110..9131
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9248..9265)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9419..10007)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9499..9518)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10178..10969,10970..11038))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10801..10820
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11039..11143)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatccgcc tttatgtgta actcttggct gaagctctta caccaatgct
       61 gggggacatg tacctcccag gggcccagga agactacggg aggctacacc aacgtcaatc
      121 agaggggcct gtgtagctac cgataagcgg accctcaaga gggcattagc aatagtgttt
      181 ataaggcccc cttgttaacc ctaaacgggt agcatatgct tcccgggtag tagtatatac
      241 tatccagact aaccctaatt caatagcata tgttacccaa cgggaagcat atgctatcga
      301 attagggtta gtaaaagggt cctaaggaac agcgatatct cccaccccat gagctgtcac
      361 ggttttattt acatggggtc aggattccac gagggtagtg aaccatttta gtcacaaggg
      421 cagtggctga agatcaagga gcgggcagtg aactctcctg aatcttcgcc tgcttcttca
      481 ttctccttcg tttagctaat agaataactg ctgagttgtg aacagtaagg tgtatgtgag
      541 gtgctcgaaa acaaggtttc aggtgacgcc cccagaataa aatttggacg gggggttcag
      601 tggtggcatt gtgctatgac accaatataa ccctcacaaa ccccttgggc aataaatact
      661 agtgtaggaa tgaaacattc tgaatatctt taacaataga aatccatggg gtggggacaa
      721 gccgtaaaga ctggatgtcc atctcacacg aatttatggc tatgggcaac acataatcct
      781 agtgcaatat gatactgggg ttattaagat gtgtcccagg cagggaccaa gacaggtgaa
      841 ccatgttgtt acactctatt tgtaacaagg ggaaagagag tggacgccga cagcagcgga
      901 ctccactggt tgtctctaac acccccgaaa attaaacggg gctccacgcc aatggggccc
      961 ataaacaaag acaagtggcc actctttttt ttgaaattgt ggagtggggg cacgcgtcag
     1021 cccccacacg ccgccctgcg gttttggact gtaaaataag ggtgtaataa cttggctgat
     1081 tgtaaccccg ctaaccactg cggtcaaacc acttgcccac aaaaccacta atggcacccc
     1141 ggggaatacc tgcataagta ggtgggcggg ccaagatagg ggcgcgattg ctgcgatctg
     1201 gaggacaaat tacacacact tgcgcctgag cgccaagcac agggttgttg gtcctcatat
     1261 tcacgaggtc gctgagagca cggtgggcta atgttgccat gggtagcata tactacccaa
     1321 atatctggat agcatatgct atcctaatct atatctgggt agcataggct atcctaatct
     1381 atatctgggt agcatatgct atcctaatct atatctgggt agtatatgct atcctaattt
     1441 atatctgggt agcataggct atcctaatct atatctgggt agcatatgct atcctaatct
     1501 atatctgggt agtatatgct atcctaatct gtatccgggt agcatatgct atcctaatag
     1561 agattagggt agtatatgct atcctaattt atatctgggt agcatatact acccaaatat
     1621 ctggatagca tatgctatcc taatctatat ctgggtagca tatgctatcc taatctatat
     1681 ctgggtagca taggctatcc taatctatat ctgggtagca tatgctatcc taatctatat
     1741 ctgggtagta tatgctatcc taatttatat ctgggtagca taggctatcc taatctatat
     1801 ctgggtagca tatgctatcc taatctatat ctgggtagta tatgctatcc taatctgtat
     1861 ccgggtagca tatgctatcc tcatgcatat acagtcagca tatgataccc agtagtagag
     1921 tgggagtgct atcctttgca tatgccgcca cctcccaagg gggcgtgaat tttcgctgct
     1981 tgtccttttc ctgctgggga tctcccgatc ccctatggtg cactctcagt acaatctgct
     2041 ctgatgccgc atagttaagc cagtatctgc tccctgcttg tgtgttggag gtcgctgagt
     2101 agtgcgcgag caaaatttaa gctacaacaa ggcaaggctt gaccgacaat tgcatgaaga
     2161 atctgcttag ggttaggcgt tttgcgctgc ttcgatcaca actttgtata gaaaagttgg
     2221 ccctgcaggc gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc
     2281 ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca
     2341 ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta
     2401 tcatatgcca agtccgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta
     2461 tgcccagtac atgaccttac gggactttcc tacttggcag tacatctacg tattagtcat
     2521 cgctattacc atggtgatgc ggttttggca gtacaccaat gggcgtggat agcggtttga
     2581 ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca
     2641 aaatcaacgg gactttccaa aatgtcgtaa taaccccgcc ccgttgacgc aaatgggcgg
     2701 taggcgtgta cggtgggagg tctatataag cagagctcgt ttagtgaacc gtcagatcgc
     2761 ctggagacgc catccacgct gttttgacct ccatagaaga caacaacttt gtacaaaaaa
     2821 gttggcacca tgttcatctt cctcctgttc ctgaccctga cttcgggatc cgacctcgac
     2881 agatgtacca ccttcgatga cgtgcaggct cccaactata cccagcatac ttcctcgatg
     2941 cggggagtgt actaccccga tgaaatcttc aggagcgaca ctctgtacct gactcaagac
     3001 ctcttcctgc ccttctactc gaacgtgacc ggcttccaca ccatcaacca cactttcggc
     3061 aaccccgtca tcccgttcaa ggacggaatc tacttcgccg ccaccgagaa aagcaacgtc
     3121 gtgcgcggat gggtgtttgg atccaccatg aacaacaaga gccaaagcgt gatcatcatt
     3181 aacaactcaa ccaacgtcgt gatccgggcc tgcaatttcg agctctgcga caacccgttc
     3241 ttcgcggtgt ccaagccgat gggaactcag acccacacca tgatcttcga taacgccttc
     3301 aactgtacct tcgagtacat ttccgacgcg ttctccctcg acgtcagcga aaagtcggga
     3361 aacttcaagc accttcggga gttcgtgttc aagaacaagg atggctttct gtatgtgtac
     3421 aaagggtacc agcccatcga tgtggtccgg gatctgccga gcggcttcaa caccctgaag
     3481 ccgattttca agctgcctct tggtatcaac atcacgaact ttagggccat cctcaccgct
     3541 ttctcccctg cacaagacat ctggggtact tccgccgctg cctacttcgt gggctacctg
     3601 aaacccacca ccttcatgct gaaatacgac gaaaacggaa ccatcacaga tgccgtggat
     3661 tgcagccaga acccgctggc tgagctgaag tgctcagtga agtcatttga aattgataag
     3721 gggatctatc aaacatccaa cttcagagtg gtgccgtctg gcgatgtcgt ccgcttccct
     3781 aacatcacta atctgtgccc cttcggcgaa gtgttcaacg ccactaagtt ccctagcgtg
     3841 tacgcctggg agcggaagaa gatctcgaat tgcgtggccg actactctgt gctgtacaac
     3901 tccaccttct tctcgacctt caagtgctac ggagtgtccg cgactaagct gaacgatctc
     3961 tgcttttcca acgtctacgc ggacagcttt gtcgtgaaag gggacgatgt gcggcagatc
     4021 gcgccaggac agaccggagt gattgccgac tacaattaca agctgcctga cgacttcatg
     4081 ggttgcgtcc tcgcgtggaa cacccggaac atcgacgcca cctccaccgg aaactacaac
     4141 tacaagtata gatacctccg ccacggaaag ctccggccat tcgaaagaga catctcaaac
     4201 gtccctttct ccccggacgg aaagccttgt accccgcctg cgcttaactg ctactggccc
     4261 ctgaacgact acggcttcta caccactacc ggcattggct accagcccta ccgggtggtg
     4321 gtgctgtcct tcgagctcct gaacgctcca gccactgtct gcggtcctaa gttgtccact
     4381 gatctgatta agaaccaatg cgtgaacttc aacttcaacg ggctgaccgg aaccggagtc
     4441 ttgaccccat cgtccaagcg gttccagccg ttccagcaat tcggacggga cgtgtcagac
     4501 tttaccgata gcgtgcggga ccctaagacc agcgagatcc tggacatctc gccgtgcgcg
     4561 tttggtggag tgtccgtgat tacccccggc accaatgcct cctccgaagt ggccgtgctg
     4621 taccaggatg tgaactgcac cgatgtgtcc accgccatcc acgccgacca gttgaccccc
     4681 gcctggagaa tctactcgac cggaaacaac gtgttccaga ctcaggccgg ctgtctcatc
     4741 ggtgccgagc acgtggacac ctcctatgag tgtgacatcc ctatcggcgc gggaatctgc
     4801 gcgtcctacc atactgtctc gctgctgcgc agtacctcgc aaaaatccat cgtggcgtac
     4861 accatgagcc tcggagcgga ctcatccatc gcctactcga acaacaccat cgccatccct
     4921 accaacttct ccatctctat cactaccgag gtcatgcccg tgtccatggc caagacctcg
     4981 gtggactgca atatgtacat ctgtggggac agcaccgaat gcgcgaatct gctgttgcag
     5041 tacggctcct tctgtacaca actgaaccgc gcactgtcag gcattgccgc tgagcaagac
     5101 agaaacacgc gcgaagtgtt tgcccaagtg aagcagatgt acaaaacccc gactctgaag
     5161 tactttggag gattcaattt ctcgcaaatt ctgcccgatc cgctcaagcc caccaagagg
     5221 tccttcattg aggacctcct gtttaacaag gtcacgttgg cggatgccgg gttcatgaag
     5281 cagtacggag aatgcctggg agacatcaac gcccgcgacc tcatctgcgc ccaaaagttc
     5341 aatggcctga ccgtgctgcc tccgctcctc actgatgaca tgattgccgc ttataccgcc
     5401 gcactggtgt caggaactgc aactgccggc tggaccttcg gtgctggcgc tgcacttcaa
     5461 attccgttcg ccatgcaaat ggcctaccgg ttcaacggca ttggcgtgac tcagaacgtc
     5521 ctgtacgaga accaaaagca aatcgccaat cagttcaaca aggccatcag ccagatccag
     5581 gagtccctca ccaccacttc gaccgccctg ggaaagctgc aggacgtggt caaccagaac
     5641 gcgcaggccc tcaacaccct cgtgaagcag ctgagctcca acttcggcgc aatctcctcg
     5701 gtgctgaacg atattctttc acgcctggac cctccagagg ccgaggtgca aatcgacaga
     5761 ctgattactg gacggctgca gagcctgcag acctacgtga ctcagcaact gatcagagcc
     5821 gcggaaatcc gagccagcgc taacctcgct gcaaccaaga tgtctgaatg cgtgctgggg
     5881 cagtcgaagc gggtggactt ctgcggaaag gggtaccacc tgatgagctt cccacaagct
     5941 gccccacacg gagtggtctt tctgcacgtg acctacgtgc ctagccaaga gcgcaacttt
     6001 accaccgcac cggctatttg ccatgaaggg aaggcgtact tcccacgcga aggagtgttc
     6061 gtgtttaacg gcacctcctg gttcattact cagcgcaact tctttagccc ccaaattatc
     6121 accaccgaca acacctttgt ctccggaaac tgcgacgtgg tcatcgggat cataaacaac
     6181 actgtgtacg atcctctgca acccgagctg gatagtttca aggaagaact cgacaagtac
     6241 ttcaagaatc acacgtcccc ggatgtcgac ctgggcgata ttagcggtat caacgcctcc
     6301 gtggtgaaca ttcagaagga aatcgacagg ctgaacgaag tcgccaagaa cctgaacgaa
     6361 agcctgatcg accttcagga acttgggaaa tacgaacagg gcagcggtta cattcccgaa
     6421 gccccgagag atggacaggc ctacgtgcgc aaggacggag aatgggtgct gctgtccact
     6481 tttctgggtc ggtccctcga ggtgctgttc cagggtccgg gtcaccatca ccaccaccat
     6541 catcactccg catggagcca cccgcagttc gaaaagggag gaggatccgg aggaggaggt
     6601 tcagggggat ccgcctggag ccatccccag ttcgagaagt aatcaacttt cttgtacaaa
     6661 gttgtgaaat tctgcagata tccatcacac tggcggccgc tcgagcatgc atctagaggg
     6721 ccctattcta tagtgtcacc taaatgctag agctcgctga tcagcctcga ctgtgccttc
     6781 tagttgccag ccatctgttg tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc
     6841 cactcccact gtcctttcct aataaaatga ggaaattgca tcgcattgtc tgagtaggtg
     6901 tcattctatt ctggggggtg gggtggggca ggacagcaag ggggaggatt gggaagacaa
     6961 tagcaggcat gctggggatg cggtgggctc tatggcttct gaggcggaaa gaaccagctg
     7021 gggctctagg gggtatcccc acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt
     7081 ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt
     7141 cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct
     7201 ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg
     7261 tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga
     7321 gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc
     7381 ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga
     7441 gctgatttaa caaaaattta acgcgaatta attctgtgga atgtgtgtca gttagggtgt
     7501 ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca
     7561 gcaaccaggt gtggaaagtc cctaggctcc ccagcaggca gaagtatgca aagcatgcat
     7621 ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg
     7681 cccagttccg cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc
     7741 gaggccgcct ctgcctctga gctattccag aagtagtgag gaggcttttt tggaggccta
     7801 ggcttttgca aaaagctccc gggagcttgt atatccattt tcggatctga tcaagagaca
     7861 ggatgaggat cgtttcgcat gattgaacaa gatggattgc acgcaggttc tccggccgct
     7921 tgggtggaga ggctattcgg ctatgactgg gcacaacaga caatcggctg ctctgatgcc
     7981 gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc
     8041 ggtgccctga atgaactgca ggacgaggca gcgcggctat cgtggctggc cacgacgggc
     8101 gttccttgcg cagctgtgct cgacgttgtc actgaagcgg gaagggactg gctgctattg
     8161 ggcgaagtgc cggggcagga tctcctgtca tctcaccttg ctcctgccga gaaagtatcc
     8221 atcatggctg atgcaatgcg gcggctgcat acgcttgatc cggctacctg cccattcgac
     8281 caccaagcga aacatcgcat cgagcgagca cgtactcgga tggaagccgg tcttgtcgat
     8341 caggatgatc tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc
     8401 aaggcgcgca tgcccgacgg cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg
     8461 aatatcatgg tggaaaatgg ccgcttttct ggattcatcg actgtggccg gctgggtgtg
     8521 gcggaccgct atcaggacat agcgttggct acccgtgata ttgctgaaga gcttggcggc
     8581 gaatgggctg accgcttcct cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc
     8641 gccttctatc gccttcttga cgagttcttc tgagcgggac tctggggttc gaaatgaccg
     8701 accaagcgac gcccaacctg ccatcacgag atttcgattc caccgccgcc ttctatgaaa
     8761 ggttgggctt cggaatcgtt ttccgggacg ccggctggat gatcctccag cgcggggatc
     8821 tcatgctgga gttcttcgcc caccccaact tgtttattgc agcttataat ggttacaaat
     8881 aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg
     8941 gtttgtccaa actcatcaat gtatcttatc atgtctgtat accgtcgacc tctagctaga
     9001 gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc
     9061 cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct
     9121 aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc
     9181 agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt
     9241 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag
     9301 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca
     9361 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt
     9421 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
     9481 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
     9541 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
     9601 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
     9661 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
     9721 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
     9781 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
     9841 actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct
     9901 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt
     9961 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
    10021 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca
    10081 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat
    10141 caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg
    10201 cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt
    10261 agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag
    10321 acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc
    10381 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag
    10441 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca
    10501 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa
    10561 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga
    10621 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata
    10681 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca
    10741 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg
    10801 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg
    10861 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg
    10921 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag
    10981 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac
    11041 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca
    11101 tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag
    11161 tgccacctga cgtc
//
