LOCUS       sequence-327683-       13100 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Introducing GAL promoters-controlled monterpene limonene-producing 
            module and Y-FAST and in S. cerevisiae .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13100)
  AUTHORS   Lu Z, Peng B, Ebert BE, Dumsday G, Vickers CE
  TITLE     Auxin-mediated protein depletion for metabolic engineering in 
            terpene-producing yeast.
  JOURNAL   Nat Commun. 2021 Feb 16;12(1):1051. doi: 10.1038/s41467-021-21313-1.
  PUBMED    33594068
REFERENCE   2  (bases 1 to 13100)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2021-02-16"; volume: "12(1):1051. doi"; pages: " 
            10.1038/s41467-021-21313-1"
COMMENT     Sequence Label: pJT11F
FEATURES             Location/Qualifiers
     source          1..13100
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(28..616)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(108..127)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(787..1578,1579..1647))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1410..1429
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1648..1752)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      1779..3121
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     protein_bind    complement(2742..2789)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     primer_bind     3162..3180
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(3218..3240)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     3340..3359
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     CDS             complement(3853..4947)
                     /codon_start=1
                     /gene="S. cerevisiae LEU2"
                     /product="3-isopropylmalate dehydrogenase, required for 
                     leucine biosynthesis"
                     /label=LEU2
                     /note="yeast auxotrophic marker"
                     /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL
                     IGGAAIDATGVPLPDEALEASKKADAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQLYA
                     NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT
                     VPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLKVQHQ
                     LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF
                     GLYEPCHGSAPDLPKNKVNPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG
                     DLGGSNSTTEVGDAVAEEVKKILA"
     promoter        complement(4948..5355)
                     /gene="S. cerevisiae LEU2"
                     /label=LEU2 promoter
     rep_origin      complement(5656..6111)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5793..5814)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     6005..6024
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6237..6259
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     6251..6268
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     6252..6268
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6278..6297
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        6278..6296
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(7709..8150)
                     /gene="S. cerevisiae GAL1"
                     /label=GAL1 promoter
                     /note="inducible promoter, regulated by Gal4"
     primer_bind     complement(7715..7738)
                     /label=GAL1
                     /note="S. cerevisiae GAL1 promoter, forward primer"
     protein_bind    8033..8150
                     /label=UAS
                     /bound_moiety="Gal4"
                     /note="upstream activating sequence mediating 
                     Gal4-dependent induction"
     CDS             8367..8741
                     /codon_start=1
                     /product="small protein tag, derived from photoactive 
                     yellow protein, that allows in vivo fluorescent labeling by
                     addition of a cell-permeant fluorogen (Plamont et al., 
                     2016)"
                     /label=Y-FAST
                     /translation="MEHVAFGSEDIENTLAKMDDGQLDGLAFGAIQLDGDGNILQYNAA
                     EGDITGRDPKQVIGKNFFKDVAPGTDSPEFYGKFKEGVASGNLNTMFEWMIPTSRGPTK
                     VKVHMKKALSGDSYWVFVKRV"
     primer_bind     complement(12687..12707)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(12687..12705)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(12726..12742)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(12726..12742)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(12739..12761)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    12750..12766
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(12774..12780,12781..12798,12799..12804))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12819..12840
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(12957..12974)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca
       61 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
      121 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
      181 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta
      241 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
      301 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
      361 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
      421 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg
      481 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
      541 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
      601 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
      661 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat
      721 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc
      781 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc
      841 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc
      901 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc
      961 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc
     1021 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt
     1081 gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc
     1141 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa
     1201 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt
     1261 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg
     1321 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc
     1381 gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa
     1441 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt
     1501 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt
     1561 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag
     1621 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta
     1681 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat
     1741 aggggttccg cgcacatttc cccgaaaagt gccacctgaa cgaagcatct gtgcttcatt
     1801 ttgtagaaca aaaatgcaac gcgagagcgc taatttttca aacaaagaat ctgagctgca
     1861 tttttacaga acagaaatgc aacgcgaaag cgctatttta ccaacgaaga atctgtgctt
     1921 catttttgta aaacaaaaat gcaacgcgag agcgctaatt tttcaaacaa agaatctgag
     1981 ctgcattttt acagaacaga aatgcaacgc gagagcgcta ttttaccaac aaagaatcta
     2041 tacttctttt ttgttctaca aaaatgcatc ccgagagcgc tatttttcta acaaagcatc
     2101 ttagattact ttttttctcc tttgtgcgct ctataatgca gtctcttgat aactttttgc
     2161 actgtaggtc cgttaaggtt agaagaaggc tactttggtg tctattttct cttccataaa
     2221 aaaagcctga ctccacttcc cgcgtttact gattactagc gaagctgcgg gtgcattttt
     2281 tcaagataaa ggcatccccg attatattct ataccgatgt ggattgcgca tactttgtga
     2341 acagaaagtg atagcgttga tgattcttca ttggtcagaa aattatgaac ggtttcttct
     2401 attttgtctc tatatactac gtataggaaa tgtttacatt ttcgtattgt tttcgattca
     2461 ctctatgaat agttcttact acaatttttt tgtctaaaga gtaatactag agataaacat
     2521 aaaaaatgta gaggtcgagt ttagatgcaa gttcaaggag cgaaaggtgg atgggtaggt
     2581 tatataggga tatagcacag agatatatag caaagagata cttttgagca atgtttgtgg
     2641 aagcggtatt cgcaatattt tagtagctcg ttacagtccg gtgcgttttt ggttttttga
     2701 aagtgcgtct tcagagcgct tttggttttc aaaagcgctc tgaagttcct atactttcta
     2761 gagaatagga acttcggaat aggaacttca aagcgtttcc gaaaacgagc gcttccgaaa
     2821 atgcaacgcg agctgcgcac atacagctca ctgttcacgt cgcacctata tctgcgtgtt
     2881 gcctgtatat atatatacat gagaagaacg gcatagtgcg tgtttatgct taaatgcgta
     2941 cttatatgcg tctatttatg taggatgaaa ggtagtctag tacctcctgt gatattatcc
     3001 cattccatgc ggggtatcgt atgcttcctt cagcactacc ctttagctgt tctatatgct
     3061 gccactcctc aattggatta gtctcatcct tcaatgctat catttccttt gatattggat
     3121 catactaaga aaccattatt atcatgacat taacctataa aaataggcgt atcacgaggc
     3181 cctttcgtct cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg
     3241 agacggtcac agcttgtctg taagcggatg ccgggagcag acaagcccgt cagggcgcgt
     3301 cagcgggtgt tggcgggtgt cggggctggc ttaactatgc ggcatcagag cagattgtac
     3361 tgagagtgca ccatatcgac tacgtcgtta aggccgtttc tgacagagta aaattcttga
     3421 gggaactttc accattatgg gaaatggttc aagaaggtat tgacttaaac tccatcaaat
     3481 ggtcaggtca ttgagtgttt tttatttgtt gtattttttt ttttttagag aaaatcctcc
     3541 aatatataaa ttaggaatca tagtttcatg attttctgtt acacctaact ttttgtgtgg
     3601 tgccctcctc cttgtcaata ttaatgttaa agtgcaattc tttttcctta tcacgttgag
     3661 ccattagtat caatttgctt acctgtattc ctttacatcc tcctttttct ccttcttgat
     3721 aaatgtatgt agattgcgta tatagtttcg tctaccctat gaacatattc cattttgtaa
     3781 tttcgtgtcg tttctattat gaatttcatt tataaagttt atgtacaaat atcataaaaa
     3841 aagagaatct ttttaagcaa ggattttctt aacttcttcg gcgacagcat caccgacttc
     3901 ggtggtactg ttggaaccac ctaaatcacc agttctgata cctgcatcca aaaccttttt
     3961 aactgcatct tcaatggcct taccttcttc aggcaagttc aatgacaatt tcaacatcat
     4021 tgcagcagac aagatagtgg cgatagggtt gaccttattc tttggcaaat ctggagcaga
     4081 accgtggcat ggttcgtaca aaccaaatgc ggtgttcttg tctggcaaag aggccaagga
     4141 cgcagatggc aacaaaccca aggaacctgg gataacggag gcttcatcgg agatgatatc
     4201 accaaacatg ttgctggtga ttataatacc atttaggtgg gttgggttct taactaggat
     4261 catggcggca gaatcaatca attgatgttg aaccttcaat gtagggaatt cgttcttgat
     4321 ggtttcctcc acagtttttc tccataatct tgaagaggcc aaaacattag ctttatccaa
     4381 ggaccaaata ggcaatggtg gctcatgttg tagggccatg aaagcggcca ttcttgtgat
     4441 tctttgcact tctggaacgg tgtattgttc actatcccaa gcgacaccat caccatcgtc
     4501 ttcctttctc ttaccaaagt aaatacctcc cactaattct ctgacaacaa cgaagtcagt
     4561 acctttagca aattgtggct tgattggaga taagtctaaa agagagtcgg atgcaaagtt
     4621 acatggtctt aagttggcgt acaattgaag ttctttacgg atttttagta aaccttgttc
     4681 aggtctaaca ctaccggtac cccatttagg accacccaca gcacctaaca aaacggcatc
     4741 agccttcttg gaggcttcca gcgcctcatc tggaagtgga acacctgtag catcgatagc
     4801 agcaccacca attaaatgat tttcgaaatc gaacttgaca ttggaacgaa catcagaaat
     4861 agctttaaga accttaatgg cttcggctgt gatttcttga ccaacgtggt cacctggcaa
     4921 aacgacgatc ttcttagggg cagacattag aatggtatat ccttgaaata tatatatata
     4981 tattgctgaa atgtaaaagg taagaaaagt tagaaagtaa gacgattgct aaccacctat
     5041 tggaaaaaac aataggtcct taaataatat tgtcaacttc aagtattgtg atgcaagcat
     5101 ttagtcatga acgcttctct attctatatg aaaagccggt tccggcgctc tcacctttcc
     5161 tttttctccc aatttttcag ttgaaaaagg tatatgcgtc aggcgacctc tgaaattaac
     5221 aaaaaatttc cagtcatcga atttgattct gtgcgatagc gcccctgtgt gttctcgtta
     5281 tgttgaggaa aaaaataatg gttgctaaga gattcgaact cttgcatctt acgatacctg
     5341 agtattccca cagttaactg cggtcaagat atttcttgaa tcaggcgcct tagaccgctc
     5401 ggccaaacaa ccaattactt gttgagaaat agagtataat tatcctataa atataacgtt
     5461 tttgaacaca catgaacaag gaagtacagg acaattgatt ttgaagagaa tgtggatttt
     5521 gatgtaattg ttgggattcc atttttaata aggcaataat attaggtatg tagatatact
     5581 agaagttctc ctcgaccggt cgatatgcgg tgtgaaatac cgcacagatg cgtaaggaga
     5641 aaataccgca tcaggaaatt gtaagcgtta atattttgtt aaaattcgcg ttaaattttt
     5701 gttaaatcag ctcatttttt aaccaatagg ccgaaatcgg caaaatccct tataaatcaa
     5761 aagaatagac cgagataggg ttgagtgttg ttccagtttg gaacaagagt ccactattaa
     5821 agaacgtgga ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat ggcccactac
     5881 gtgaaccatc accctaatca agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga
     5941 accctaaagg gagcccccga tttagagctt gacggggaaa gccggcgaac gtggcgagaa
     6001 aggaagggaa gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc
     6061 tgcgcgtaac caccacaccc gccgcgctta atgcgccgct acagggcgcg tccattcgcc
     6121 attcaggctg cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc tattacgcca
     6181 gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg gtaacgccag ggttttccca
     6241 gtcacgacgt tgtaaaacga cggccagtga gcgcgcgtaa tacgactcac tatagggcga
     6301 attgggtacc gggcccattg tagcaaagat tgtaaggaaa tagcaaacca gaggtccaag
     6361 tcaaaacggc aaaaaagaag aaagtaagca atataatgga cgggtttaca tgtttaaata
     6421 tatgtacatg tatgtagtct tatactttca aaacataatc gcatgcgggt catcaaaata
     6481 aacctccatt ttggacttgg gaaaaagaaa atttgattta aactgttgta tgtagctaac
     6541 aatacataaa actatttaaa taaaatagga ataagaatgc tcaattaaaa aatgatttat
     6601 tttctaacaa aacttcctgc aggtcactat tatttgcttc tcttgtaaac tttgttcaag
     6661 aacgcagtta agacatcagc tttgaagcca cgagactcat cgacctgaga aattttggcc
     6721 ttcaaatcct tggcaataga ctcttcatat tcgtggtata gctgttcaat tttcaagtca
     6781 ttgaaaatct ttttgcattt ggcttctgcg actgagtcct tcttaccgta attttcgtct
     6841 aaagtctttc tttgttctgc ggaagcaagt tctaatgcct tgttgattac ccaagaacat
     6901 ttgttatctt ggatatctgt accgatctta ccgatctgtt ctggggtacc gaagcagtct
     6961 aagtagtcat cttgaatttg gaagtattca cccaatggaa tcaagacatc tctggcttgt
     7021 ttcaaatcct tttcatccgt gataccggca acgtacatgg ccaatgcgac aggcaagtag
     7081 aaagaatagt aagcagtctt gaaagtaact atgaaggagt gcttctttag ggagaactta
     7141 ctcaagtcga ctttgtcttc aggtgcagtg attaagtcca tcaattggcc caattcggtt
     7201 tggaaggtga cctcatggaa caattcggtg atatctatgt agtatttttc gtttctgaag
     7261 tgagatttca aaagcttgta gatagcagcc tctaacatga atgcgtccca gatggcaatt
     7321 tccccaactt caggaacctt gtaccaacat ggttggcctc ttctggtaat ggacttgtcc
     7381 atcatatcat cggcgaccaa gaagtaagcc tgcaacaact caatgcacca acctagaatg
     7441 gcaacctttt cgtattcttc ttgccccaat tgttcaacgg tcttgttgga gagaatagca
     7501 tacgtgtcca caacggacaa acctctattt agcttaccgc ctggagtgtt gtagttcaat
     7561 gagtgggcat accagtcaca tgcttcctta ggcataccgt aagccaaaag cgatgcgttc
     7621 aattcctcta ctaatttagg gaaaacgttc aagaatctct ctctcctaat ttctttttct
     7681 gaagccattt tgctagctat agttttttct ccttgacgtt aaagtataga ggtatattaa
     7741 caattttttg ttgatacttt tatgacattt gaataagaag taatacaaac cgaaaatgtt
     7801 gaaagtatta gttaaagtgg ttatgcagct tttgcattta tatatctgtt aatagatcaa
     7861 aaatcatcgc ttcgctgatt aattacccca gaaataaggc taaaaaacta atcgcattat
     7921 tatcctatgg ttgttaattt gattcgttga tttgaaggtt tgtggggcca ggttactgcc
     7981 aatttttcct cttcataacc ataaaagcta gtattgtaga atctttattg ttcggagcag
     8041 tgcggcgcga ggcacatctg cgtttcagga acgcgaccgg tgaagaccag gacgcacgga
     8101 ggagagtctt ccgtcggagg gctgtcgccc gctcggcggc ttctaatccg tacttcaata
     8161 tagcaatgag cagttaagcg tattactgaa agttccaaag agaaggtttt tttaggctaa
     8221 gataatgggg ctctttacat ttccacaaca tataagtaag attagatatg gatatgtata
     8281 tggtggtatt gccatgtaat atgattatta aacttctttg cgtccatcca aaaaaaaagt
     8341 aagaattttt gaaaattcaa tataaaatgg aacacgttgc tttcggttct gaagatattg
     8401 aaaacacttt ggctaaaatg gatgatggtc aattagacgg tttagctttc ggtgctattc
     8461 aattggacgg tgatggtaac attttgcaat acaatgctgc tgaaggtgat attactggta
     8521 gagatccaaa acaagttatt ggtaagaact tctttaagga cgttgctcca ggtactgact
     8581 ctccagagtt ctatggtaag tttaaggaag gtgttgcttc tggtaactta aacactatgt
     8641 ttgaatggat gatcccaact tccagaggtc caactaaggt taaagttcac atgaaaaagg
     8701 ctttatctgg tgactcttac tgggttttcg ttaaaagagt ttaataaagc cctatacttt
     8761 gcttctttat gtaagaaggt tggtattcca gctggtgtcg tcaacatcgt tccaggtcct
     8821 ggtagaactg ttggtgctgc tttgaccaac gacccaagaa tcagaaagct ggcttttacc
     8881 ggttctacag aagtcggtaa gagtgttgct gtcgactctt ctgaatctaa cttgaagaaa
     8941 atcactttgg aactaggtgg taagtccgcc catttggtct ttgacgatgc taacattaag
     9001 aagactttac caaatctagt aaacggtatt ttcaagaacg ctggtcaaat ttgttcctct
     9061 ggttctagaa tttacgttca agaaggtatt tacgacgaac tattggctgc tttcaaggct
     9121 tacttggaaa ccgaaatcaa agttggtaat ccatttgaca aggctaactt ccaaggtgct
     9181 atcactaacc gtcaacaatt cgacacaatt atgaactaca tcgatatcgg taagaaagaa
     9241 ggcgccaaga tcttaactgg tggcgaaaaa gttggtgaca agggttactt catcagacca
     9301 accgttttct acgatgttaa tgaagacatg agaattgtta aggaagaaat ttttggacca
     9361 gttgtcactg tcgcaaagtt caagacttta gaagaaggtg tcgaaatggc taacagctct
     9421 gaattcggtc taggttctgg tatcgaaaca gaatctttga gcacaggttt gaaggtggcc
     9481 aagatgttga aggccggtac cgtctggatc aacacataca acgattttga ctccagagtt
     9541 ccattcggtg gtgttaagca atctggttac ggtagagaaa tgggtgaaga agtctaccat
     9601 gcatacactg aagtaaaagc tgtcagaatt aagttgtaat gtaccaacct gcatttcttt
     9661 ccgtcatata cacaaaatac tttcatataa acttacttgg tcttacgtca taaataaata
     9721 tgtatacata taaattaaaa aatttggttt tatattttta caaaaagaat cgtttacttc
     9781 atttctccct tttaagcgat acaatccatg aaaaaagaga aaaagagaga acaggcttgt
     9841 gccttcttta aaacatccca cacaaaatca tattgaattg aattttacat cttaagctag
     9901 tgtacaacaa ctgctatatc caaagaaaac taacctcgag tgtactaatc caaggaggtt
     9961 tacggaccag aggaactttc cagattcaga tcacagcaat ataggactgg aaaacatcag
    10021 gtagccgcac tcaacttgta actggcaact actttgcatt aaactccaat taaatgcggt
    10081 agaatctttt cagaaaaggt attcaacgtc aattcggaaa gcttccttcc ggaatggctt
    10141 aagtaggttg caatttcttt ttctattagt agctaaaaat gggtcacgtg atctatattc
    10201 gaaaggggcg gttgcctcag gaaggcaccg gcggtctttc gtccgtgcgg agatatctgc
    10261 gccgttcagg ggtccatgtg ccttggacga tattaaggca gaaggcagta tcggggcgga
    10321 tcactccgaa ccgagattag ttaagccctt cccatctcaa gatggggagc aaatggcatt
    10381 atactcctgc tagaaagtta actgtgcaca tattcttaaa ttatacaatg ttctggagag
    10441 ctattgttta aaaaacaaac atttcgcagg ctaaaatgtg gagataggat tagttttgta
    10501 gacatatata aacaatcagt aattggattg aaaatttggt gttgtgaatt gctcttcatt
    10561 atgcacctta ttcaattatc atcaagaata gcaatagtta agtaaacaca agattaacat
    10621 aataaaaaaa ataattcttt cataggatct aaaatgagaa gatccgctaa ctatcaacca
    10681 tccatttggg accacgactt tttacaatcc ttgaactcta actacaccga cgaagcatac
    10741 aagagaagag cagaagaatt acgtggtaaa gtaaagatag ccatcaaaga tgtcatcgaa
    10801 cctttggacc aattggaatt gattgataac ttgcaaagat tgggtttagc ccatagattt
    10861 gaaaccgaaa tcagaaacat cttgaacaac atctataaca acaataagga ttacaactgg
    10921 agaaaggaaa atttgtacgc tacttccttg gaattcagat tgttaagaca acacggttac
    10981 ccagtcagtc aagaagtttt taacggtttc aaggatgacc aaggtggttt tatttgtgat
    11041 gacttcaagg gtatattgtc cttgcatgaa gcttcttact actcattgga aggtgaaagt
    11101 ataatggaag aagcatggca attcacttcc aaacacttga aggaagttat gataagtaaa
    11161 aatatggaag aagatgtttt cgtagctgaa caagcaaaga gagccttgga attaccattg
    11221 cattggaaag ttcctatgtt ggaagcaaga tggttcatcc atatatacga aagaagagaa
    11281 gataagaacc acttgttgtt ggaattggca aagatggaat tcaatacatt acaagccatc
    11341 tatcaagaag aattgaagga aatctctggt tggtggaagg ataccggttt gggtgaaaag
    11401 ttgtcattcg ctagaaatag attggtcgca tctttcttat ggtcaatggg tattgccttt
    11461 gaacctcaat tcgcttactg tagaagagtt ttgacaatct ctattgcatt gatcaccgtt
    11521 atagatgaca tctatgacgt atacggtact ttggatgaat tggaaatctt tacagacgct
    11581 gttgaaagat gggatatcaa ctatgcatta aagcatttgc caggttacat gaagatgtgc
    11641 tttttagcct tgtacaactt cgtcaacgaa ttcgcttact acgttttgaa gcaacaagat
    11701 ttcgacttat tgttatctat taaaaacgct tggttgggtt tgatacaagc ctatttggta
    11761 gaggctaagt ggtaccattc taagtacaca cctaagttgg aagaatactt ggaaaacggt
    11821 ttagtctcaa tcactggtcc attgatcatc acaatctcct atttgagtgg tactaaccct
    11881 atcattaaaa aggaattgga attcttggaa tcaaacccag atatcgttca ctggtcttca
    11941 aaaattttca gattgcaaga tgacttaggt acttccagtg acgaaattca aagaggtgac
    12001 gtacctaaat ctatacaatg ctacatgcat gaaacaggtg catcagaaga agttgccaga
    12061 caacacatta aggatatgat gagacaaatg tggaaaaagg ttaatgctta caccgcagat
    12121 aaagactccc cattgactgg tactacaacc gaattcttgt tgaacttagt tagaatgtct
    12181 catttcatgt atttgcatgg tgacggtcac ggtgtacaaa atcaagaaac cattgatgtc
    12241 ggttttactt tgttattcca acctattcca ttagaagata aacacatggc attcaccgca
    12301 tcaccaggta caaagggtta atagtgacca tggcggattg agagcaaatc gttaagttca
    12361 ggtcaagtaa aaattgattt cgaaaactaa tttctcttat acaatccttt gattggaccg
    12421 tcatcctttc gaatataaga ttttgttaag aatattttag acagagatct actttatatt
    12481 taatatctag atattacata atttcctctc taataaaata tcattaataa aataaaaatg
    12541 aagcgatttg attttgtgtt gtcaacttag tttgccgcta tgcctcttgg gtaatgctat
    12601 tattgaatcg aagggcttta ttatattacc ctttagctta ttctgaggtt tctgtggcgt
    12661 ccgcggtgga gctccagctt ttgttccctt tagtgagggt taattgcgcg cttggcgtaa
    12721 tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata
    12781 cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta
    12841 attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa
    12901 tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg
    12961 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag
    13021 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa
    13081 ggccagcaaa aggccaggaa
//
