LOCUS       sequence-327691-       13211 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Introducing GAL promoters-controlled sequiterpene 
            nerolidol-producing module and Y-FAST and in S. cerevisiae .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13211)
  AUTHORS   Lu Z, Peng B, Ebert BE, Dumsday G, Vickers CE
  TITLE     Auxin-mediated protein depletion for metabolic engineering in 
            terpene-producing yeast.
  JOURNAL   Nat Commun. 2021 Feb 16;12(1):1051. doi: 10.1038/s41467-021-21313-1.
  PUBMED    33594068
REFERENCE   2  (bases 1 to 13211)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2021-02-16"; volume: "12(1):1051. doi"; pages: " 
            10.1038/s41467-021-21313-1"
COMMENT     Sequence Label: pJT9RFR
FEATURES             Location/Qualifiers
     source          1..13211
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(2085..2105)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(2085..2103)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(2124..2140)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2124..2140)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2137..2159)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2148..2164
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2172..2178,2179..2196,2197..2202))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2217..2238
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2355..2372)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2526..3114)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2606..2625)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3285..4076,4077..4145))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3908..3927
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4146..4250)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      4277..5619
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     protein_bind    complement(5240..5287)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     primer_bind     5660..5678
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(5716..5738)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     5838..5857
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     CDS             complement(6351..7445)
                     /codon_start=1
                     /gene="S. cerevisiae LEU2"
                     /product="3-isopropylmalate dehydrogenase, required for 
                     leucine biosynthesis"
                     /label=LEU2
                     /note="yeast auxotrophic marker"
                     /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL
                     IGGAAIDATGVPLPDEALEASKKADAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQLYA
                     NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT
                     VPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLKVQHQ
                     LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF
                     GLYEPCHGSAPDLPKNKVNPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG
                     DLGGSNSTTEVGDAVAEEVKKILA"
     promoter        complement(7446..7853)
                     /gene="S. cerevisiae LEU2"
                     /label=LEU2 promoter
     rep_origin      complement(8154..8609)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8291..8312)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     8503..8522
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8735..8757
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     8749..8766
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     8750..8766
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8776..8795
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        8776..8794
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(10207..10648)
                     /gene="S. cerevisiae GAL1"
                     /label=GAL1 promoter
                     /note="inducible promoter, regulated by Gal4"
     primer_bind     complement(10213..10236)
                     /label=GAL1
                     /note="S. cerevisiae GAL1 promoter, forward primer"
     protein_bind    10531..10648
                     /label=UAS
                     /bound_moiety="Gal4"
                     /note="upstream activating sequence mediating 
                     Gal4-dependent induction"
     CDS             12777..13151
                     /codon_start=1
                     /product="small protein tag, derived from photoactive 
                     yellow protein, that allows in vivo fluorescent labeling by
                     addition of a cell-permeant fluorogen (Plamont et al., 
                     2016)"
                     /label=Y-FAST
                     /translation="MEHVAFGSEDIENTLAKMDDGQLDGLAFGAIQLDGDGNILQYNAA
                     EGDITGRDPKQVIGKNFFKDVAPGTDSPEFYGKFKEGVASGNLNTMFEWMIPTSRGPTK
                     VKVHMKKALSGDSYWVFVKRV"
ORIGIN
        1 gctaccgcag caggtcctat cgcaactaac aactccccac aaaactccaa cgcttacaga
       61 actccaatcg ctccttccgt accaattact cataaatggt ctatagctga agatttgaca
      121 tgtatttcca atcctagtaa gcacaataac cctcaaactg gttacagatc attttctgac
      181 gaattatacg ttaagtacga agaaaagttg gaagatgtta gaaaagcatt aagagaagtt
      241 gaagaaaacc ctttggaagg tttagttatg atagacgctt tgcaaagatt gggtatcgat
      301 taccatttca gaggtgaaat tggtgcattc ttgcaaaagc aacaaatcat atcttcaact
      361 ccagatggtt accctgaaca tggtttgtac gaagtttcaa cattgtttag attcttaaga
      421 caagaaggtc acaatgttac cgctgacgtc tttaataact tcaaggataa ggaaggtaga
      481 ttcagatcag aattgtcaac agatattaga ggtttgatgt ccttatacga agcaagtcaa
      541 ttgagaatag aaggtgaaga catcttagat caagctgctg atttctccag tcaattgtta
      601 ggtagatgga caaaagatcc taatcatcac gaagccagat tggtttctaa cactttaaca
      661 catccatacc acaagtcatt ggctaccttt atgggtcaaa aattgtccta catgaactgc
      721 aagggtccaa actgggacgg tgtcgataat ttgcaagaat tagctaagat ggatttgact
      781 atcgtacaaa gtatccatca aaaggaagta ttccaagttt ctcaatggtg gaaggataca
      841 ggtttagcca atgaattgaa attggctaga aaccaaccat tgaagtggta tatgtggcct
      901 atggccgctt taaccgatcc aagattcagt gaagaaagag ttgaattaac taagcctatt
      961 tcttttatat acatcataga tgacatcttc gacgtctatg gtaccattga agaattgacc
     1021 ttgtttactg atgcagttaa tagatgggaa ttgtctgccg tcgaacaatt accagactac
     1081 atgaaagtat gtttcaaggc tttgtacgat gttaccaacg aaatcgcata caaaatctat
     1141 aaaaagcatg gtcaaaaccc tattgattcc ttgcaaaaga cttgggctag tttgtgcaat
     1201 gcatttttag ttgaagcaaa gtggttcgcc tctggtcact tgccaaatgc agaagaatac
     1261 ttaaagaacg gtatcatctc ttcaggtgtt catgttgtct tggcccacat gtttttcttg
     1321 ttaggtgacg gtattacaca agaatcagtt gatttggtag atgactatcc aggtatttcc
     1381 acaagtatcg caaccatttt gagattatct gatgacttgg gttcagccaa agatgaagac
     1441 caagatggtt atgatggttc ttacatcgaa tgttacatga aggaacataa gggttccagt
     1501 gtcgattcag ccagagaaga agtaataaga atgatctccg aagcatggaa atgtttgaat
     1561 aaggaatgct tatcaccaaa ccctttttct gaatcattca gaataggttc cttgaatatg
     1621 gctagaatga tccctatgat gtactcttac gatgacaacc ataacttgcc aattttagaa
     1681 gaacacatga aggcaatgat ctataacaca tctttataat agtgaccatg gcggattgag
     1741 agcaaatcgt taagttcagg tcaagtaaaa attgatttcg aaaactaatt tctcttatac
     1801 aatcctttga ttggaccgtc atcctttcga atataagatt ttgttaagaa tattttagac
     1861 agagatctac tttatattta atatctagat attacataat ttcctctcta ataaaatatc
     1921 attaataaaa taaaaatgaa gcgatttgat tttgtgttgt caacttagtt tgccgctatg
     1981 cctcttgggt aatgctatta ttgaatcgaa gggctttatt atattaccct ttagcttatt
     2041 ctgaggtttc tgtggcgtcc gcggtggagc tccagctttt gttcccttta gtgagggtta
     2101 attgcgcgct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc
     2161 acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga
     2221 gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg
     2281 tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg
     2341 cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg
     2401 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga
     2461 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
     2521 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
     2581 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
     2641 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
     2701 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
     2761 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
     2821 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
     2881 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
     2941 tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca
     3001 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
     3061 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
     3121 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt
     3181 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt
     3241 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc
     3301 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc
     3361 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata
     3421 ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg
     3481 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc
     3541 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct
     3601 acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa
     3661 cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt
     3721 cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca
     3781 ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac
     3841 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca
     3901 atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt
     3961 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc
     4021 actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca
     4081 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata
     4141 ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc
     4201 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc
     4261 cgaaaagtgc cacctgaacg aagcatctgt gcttcatttt gtagaacaaa aatgcaacgc
     4321 gagagcgcta atttttcaaa caaagaatct gagctgcatt tttacagaac agaaatgcaa
     4381 cgcgaaagcg ctattttacc aacgaagaat ctgtgcttca tttttgtaaa acaaaaatgc
     4441 aacgcgagag cgctaatttt tcaaacaaag aatctgagct gcatttttac agaacagaaa
     4501 tgcaacgcga gagcgctatt ttaccaacaa agaatctata cttctttttt gttctacaaa
     4561 aatgcatccc gagagcgcta tttttctaac aaagcatctt agattacttt ttttctcctt
     4621 tgtgcgctct ataatgcagt ctcttgataa ctttttgcac tgtaggtccg ttaaggttag
     4681 aagaaggcta ctttggtgtc tattttctct tccataaaaa aagcctgact ccacttcccg
     4741 cgtttactga ttactagcga agctgcgggt gcattttttc aagataaagg catccccgat
     4801 tatattctat accgatgtgg attgcgcata ctttgtgaac agaaagtgat agcgttgatg
     4861 attcttcatt ggtcagaaaa ttatgaacgg tttcttctat tttgtctcta tatactacgt
     4921 ataggaaatg tttacatttt cgtattgttt tcgattcact ctatgaatag ttcttactac
     4981 aatttttttg tctaaagagt aatactagag ataaacataa aaaatgtaga ggtcgagttt
     5041 agatgcaagt tcaaggagcg aaaggtggat gggtaggtta tatagggata tagcacagag
     5101 atatatagca aagagatact tttgagcaat gtttgtggaa gcggtattcg caatatttta
     5161 gtagctcgtt acagtccggt gcgtttttgg ttttttgaaa gtgcgtcttc agagcgcttt
     5221 tggttttcaa aagcgctctg aagttcctat actttctaga gaataggaac ttcggaatag
     5281 gaacttcaaa gcgtttccga aaacgagcgc ttccgaaaat gcaacgcgag ctgcgcacat
     5341 acagctcact gttcacgtcg cacctatatc tgcgtgttgc ctgtatatat atatacatga
     5401 gaagaacggc atagtgcgtg tttatgctta aatgcgtact tatatgcgtc tatttatgta
     5461 ggatgaaagg tagtctagta cctcctgtga tattatccca ttccatgcgg ggtatcgtat
     5521 gcttccttca gcactaccct ttagctgttc tatatgctgc cactcctcaa ttggattagt
     5581 ctcatccttc aatgctatca tttcctttga tattggatca tactaagaaa ccattattat
     5641 catgacatta acctataaaa ataggcgtat cacgaggccc tttcgtctcg cgcgtttcgg
     5701 tgatgacggt gaaaacctct gacacatgca gctcccggag acggtcacag cttgtctgta
     5761 agcggatgcc gggagcagac aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg
     5821 gggctggctt aactatgcgg catcagagca gattgtactg agagtgcacc atatcgacta
     5881 cgtcgttaag gccgtttctg acagagtaaa attcttgagg gaactttcac cattatggga
     5941 aatggttcaa gaaggtattg acttaaactc catcaaatgg tcaggtcatt gagtgttttt
     6001 tatttgttgt attttttttt ttttagagaa aatcctccaa tatataaatt aggaatcata
     6061 gtttcatgat tttctgttac acctaacttt ttgtgtggtg ccctcctcct tgtcaatatt
     6121 aatgttaaag tgcaattctt tttccttatc acgttgagcc attagtatca atttgcttac
     6181 ctgtattcct ttacatcctc ctttttctcc ttcttgataa atgtatgtag attgcgtata
     6241 tagtttcgtc taccctatga acatattcca ttttgtaatt tcgtgtcgtt tctattatga
     6301 atttcattta taaagtttat gtacaaatat cataaaaaaa gagaatcttt ttaagcaagg
     6361 attttcttaa cttcttcggc gacagcatca ccgacttcgg tggtactgtt ggaaccacct
     6421 aaatcaccag ttctgatacc tgcatccaaa acctttttaa ctgcatcttc aatggcctta
     6481 ccttcttcag gcaagttcaa tgacaatttc aacatcattg cagcagacaa gatagtggcg
     6541 atagggttga ccttattctt tggcaaatct ggagcagaac cgtggcatgg ttcgtacaaa
     6601 ccaaatgcgg tgttcttgtc tggcaaagag gccaaggacg cagatggcaa caaacccaag
     6661 gaacctggga taacggaggc ttcatcggag atgatatcac caaacatgtt gctggtgatt
     6721 ataataccat ttaggtgggt tgggttctta actaggatca tggcggcaga atcaatcaat
     6781 tgatgttgaa ccttcaatgt agggaattcg ttcttgatgg tttcctccac agtttttctc
     6841 cataatcttg aagaggccaa aacattagct ttatccaagg accaaatagg caatggtggc
     6901 tcatgttgta gggccatgaa agcggccatt cttgtgattc tttgcacttc tggaacggtg
     6961 tattgttcac tatcccaagc gacaccatca ccatcgtctt cctttctctt accaaagtaa
     7021 atacctccca ctaattctct gacaacaacg aagtcagtac ctttagcaaa ttgtggcttg
     7081 attggagata agtctaaaag agagtcggat gcaaagttac atggtcttaa gttggcgtac
     7141 aattgaagtt ctttacggat ttttagtaaa ccttgttcag gtctaacact accggtaccc
     7201 catttaggac cacccacagc acctaacaaa acggcatcag ccttcttgga ggcttccagc
     7261 gcctcatctg gaagtggaac acctgtagca tcgatagcag caccaccaat taaatgattt
     7321 tcgaaatcga acttgacatt ggaacgaaca tcagaaatag ctttaagaac cttaatggct
     7381 tcggctgtga tttcttgacc aacgtggtca cctggcaaaa cgacgatctt cttaggggca
     7441 gacattagaa tggtatatcc ttgaaatata tatatatata ttgctgaaat gtaaaaggta
     7501 agaaaagtta gaaagtaaga cgattgctaa ccacctattg gaaaaaacaa taggtcctta
     7561 aataatattg tcaacttcaa gtattgtgat gcaagcattt agtcatgaac gcttctctat
     7621 tctatatgaa aagccggttc cggcgctctc acctttcctt tttctcccaa tttttcagtt
     7681 gaaaaaggta tatgcgtcag gcgacctctg aaattaacaa aaaatttcca gtcatcgaat
     7741 ttgattctgt gcgatagcgc ccctgtgtgt tctcgttatg ttgaggaaaa aaataatggt
     7801 tgctaagaga ttcgaactct tgcatcttac gatacctgag tattcccaca gttaactgcg
     7861 gtcaagatat ttcttgaatc aggcgcctta gaccgctcgg ccaaacaacc aattacttgt
     7921 tgagaaatag agtataatta tcctataaat ataacgtttt tgaacacaca tgaacaagga
     7981 agtacaggac aattgatttt gaagagaatg tggattttga tgtaattgtt gggattccat
     8041 ttttaataag gcaataatat taggtatgta gatatactag aagttctcct cgaccggtcg
     8101 atatgcggtg tgaaataccg cacagatgcg taaggagaaa ataccgcatc aggaaattgt
     8161 aagcgttaat attttgttaa aattcgcgtt aaatttttgt taaatcagct cattttttaa
     8221 ccaataggcc gaaatcggca aaatccctta taaatcaaaa gaatagaccg agatagggtt
     8281 gagtgttgtt ccagtttgga acaagagtcc actattaaag aacgtggact ccaacgtcaa
     8341 agggcgaaaa accgtctatc agggcgatgg cccactacgt gaaccatcac cctaatcaag
     8401 ttttttgggg tcgaggtgcc gtaaagcact aaatcggaac cctaaaggga gcccccgatt
     8461 tagagcttga cggggaaagc cggcgaacgt ggcgagaaag gaagggaaga aagcgaaagg
     8521 agcgggcgct agggcgctgg caagtgtagc ggtcacgctg cgcgtaacca ccacacccgc
     8581 cgcgcttaat gcgccgctac agggcgcgtc cattcgccat tcaggctgcg caactgttgg
     8641 gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct
     8701 gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg taaaacgacg
     8761 gccagtgagc gcgcgtaata cgactcacta tagggcgaat tgggtaccgg gcccattgta
     8821 gcaaagattg taaggaaata gcaaaccaga ggtccaagtc aaaacggcaa aaaagaagaa
     8881 agtaagcaat ataatggacg ggtttacatg tttaaatata tgtacatgta tgtagtctta
     8941 tactttcaaa acataatcgc atgcgggtca tcaaaataaa cctccatttt ggacttggga
     9001 aaaagaaaat ttgatttaaa ctgttgtatg tagctaacaa tacataaaac tatttaaata
     9061 aaataggaat aagaatgctc aattaaaaaa tgatttattt tctaacaaaa cttcctgcag
     9121 gtcactatta tttgcttctc ttgtaaactt tgttcaaaaa cgcagttaag acgtcggctt
     9181 tgaagccacg agactcgtcg acttgggaga tcttggcctt caaatccttg gcaacagact
     9241 cttcatattc gtggtataac tggtcgattt tcaagtcatt gaaaatcttt ttgcatttgg
     9301 cttctgcgac tgagtccttc ttaccgtaat tttcgtctaa agtctttctt tgttctgcgg
     9361 aagcaagttc taatgccttg ttgattaccc aagaacattt gttatcttgg atatctgtac
     9421 cgatcttacc gatctgttct ggggtaccga agcagtctaa gtagtcatct tgaatttgga
     9481 aatattcacc caatggaatc aagacatctc tggcttgttt caaatccttt tcatctgtga
     9541 taccggcaac gtacatagcc aatgcgacag gcaagtagaa agaatagtaa gcagtcttga
     9601 aagtaactat gaaggagtgc ttctttaggg agaacttact caagtcgact ttgtcttcag
     9661 gtgcagtgat taagtccatc aattggccca attcggtttg gaaggtgact tcatggaaca
     9721 attcggtgat atctatgtag tatttttcgt ttctgaagtg agatttcaaa agcttgtaga
     9781 tagcagcctc taacatgaat gcgtcattga tggcaatttc cccaacttca ggaaccttgt
     9841 accaacatgg ttggcctctt ctggtaatgg acttgtccat catatcatcg gcgaccaaga
     9901 agtaagcctg caacaactca atgcaccaac ctagaatagc aaccttttcg tattcttctt
     9961 gccccaattg ttcaacggtc ttgttggaga gaatagcata cgtgtccaca acggacaaac
    10021 ctctatttaa cttaccgcct ggagtgttgt agttcaatga gtgggcatac cagtcacatg
    10081 cttccttagg cataccgtaa gccaaaagcg atgcgttcaa ttcctctact aatttaggga
    10141 aaacgttcaa gaatctctct ctcctaattt ctttttctga agccattttg ctagctatag
    10201 ttttttctcc ttgacgttaa agtatagagg tatattaaca attttttgtt gatactttta
    10261 tgacatttga ataagaagta atacaaaccg aaaatgttga aagtattagt taaagtggtt
    10321 atgcagcttt tgcatttata tatctgttaa tagatcaaaa atcatcgctt cgctgattaa
    10381 ttaccccaga aataaggcta aaaaactaat cgcattatta tcctatggtt gttaatttga
    10441 ttcgttgatt tgaaggtttg tggggccagg ttactgccaa tttttcctct tcataaccat
    10501 aaaagctagt attgtagaat ctttattgtt cggagcagtg cggcgcgagg cacatctgcg
    10561 tttcaggaac gcgaccggtg aagaccagga cgcacggagg agagtcttcc gtcggagggc
    10621 tgtcgcccgc tcggcggctt ctaatccgta cttcaatata gcaatgagca gttaagcgta
    10681 ttactgaaag ttccaaagag aaggtttttt taggctaaga taatggggct ctttacattt
    10741 ccacaacata taagtaagat tagatatgga tatgtatatg gtggtattgc catgtaatat
    10801 gattattaaa cttctttgcg tccatccaaa aaaaaagtaa gaatttttga aaattcaata
    10861 taaactagta aagccctata ctttgcttct ttatgtaaga aggttggtat tccagctggt
    10921 gtcgtcaaca tcgttccagg tcctggtaga actgttggtg ctgctttgac caacgaccca
    10981 agaatcagaa agctggcttt taccggttct acagaagtcg gtaagagtgt tgctgtcgac
    11041 tcttctgaat ctaacttgaa gaaaatcact ttggaactag gtggtaagtc cgcccatttg
    11101 gtctttgacg atgctaacat taagaagact ttaccaaatc tagtaaacgg tattttcaag
    11161 aacgctggtc aaatttgttc ctctggttct agaatttacg ttcaagaagg tatttacgac
    11221 gaactattgg ctgctttcaa ggcttacttg gaaaccgaaa tcaaagttgg taatccattt
    11281 gacaaggcta acttccaagg tgctatcact aaccgtcaac aattcgacac aattatgaac
    11341 tacatcgata tcggtaagaa agaaggcgcc aagatcttaa ctggtggcga aaaagttggt
    11401 gacaagggtt acttcatcag accaaccgtt ttctacgatg ttaatgaaga catgagaatt
    11461 gttaaggaag aaatttttgg accagttgtc actgtcgcaa agttcaagac tttagaagaa
    11521 ggtgtcgaaa tggctaacag ctctgaattc ggtctaggtt ctggtatcga aacagaatct
    11581 ttgagcacag gtttgaaggt ggccaagatg ttgaaggccg gtaccgtctg gatcaacaca
    11641 tacaacgatt ttgactccag agttccattc ggtggtgtta agcaatctgg ttacggtaga
    11701 gaaatgggtg aagaagtcta ccatgcatac actgaagtaa aagctgtcag aattaagttg
    11761 taatgtacca acctgcattt ctttccgtca tatacacaaa atactttcat ataaacttac
    11821 ttggtcttac gtcataaata aatatgtata catataaatt aaaaaatttg gttttatatt
    11881 tttacaaaaa gaatcgttta cttcatttct cccttttaag cgatacaatc catgaaaaaa
    11941 gagaaaaaga gagaacaggc ttgtgccttc tttaaaacat cccacacaaa atcatattga
    12001 attgaatttt acatcttaag ctagtgtaca acaactgcta tatccaaaga aaataacctc
    12061 gagtgtacta atccaaggag gtttacggac cagaggaact ttccagattc agatcacagc
    12121 aatataggac tggaaaacat caggtagccg cactcaactt gtaactggca actactttgc
    12181 attaaactcc aattaaatgc ggtagaatct tttcagaaaa ggtattcaac gtcaattcgg
    12241 aaagcttcct tccggaatgg cttaagtagg ttgcaatttc tttttctatt agtagctaaa
    12301 aatgggtcac gtgatctata ttcgaaaggg gcggttgcct caggaaggca ccggcggtct
    12361 ttcgtccgtg cggagatatc tgcgccgttc aggggtccat gtgccttgga cgatattaag
    12421 gcagaaggca gtatcggggc ggatcactcc gaaccgagat tagttaagcc cttcccatct
    12481 caagatgggg agcaaatggc attatactcc tgctagaaag ttaactgtgc acatattctt
    12541 aaattataca atgttctgga gagctattgt ttaaaaaaca aacatttcgc aggctaaaat
    12601 gtggagatag gattagtttt gtagacatat ataaacaatc agtaattgga ttgaaaattt
    12661 ggtgttgtga attgctcttc attatgcacc ttattcaatt atcatcaaga atagcaatag
    12721 ttaagtaaac acaagattaa cataataaaa aaaataattc tttcagtgga tccaaaatgg
    12781 aacacgttgc tttcggttct gaagatattg aaaacacttt ggctaaaatg gatgatggtc
    12841 aattagacgg tttagctttc ggtgctattc aattggacgg tgatggtaac attttgcaat
    12901 acaatgctgc tgaaggtgat attactggta gagatccaaa acaagttatt ggtaagaact
    12961 tctttaagga cgttgctcca ggtactgact ctccagagtt ctatggtaag tttaaggaag
    13021 gtgttgcttc tggtaactta aacactatgt ttgaatggat gatcccaact tccagaggtc
    13081 caactaaggt taaagttcac atgaaaaagg ctttatctgg tgactcttac tgggttttcg
    13141 ttaaaagagt tggtgctact aacttctctt tgttgaagtt ggctggtgac gttgaactta
    13201 acccaggtcc a
//
