LOCUS       sequence-327712-        8772 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Constitutive expression (CMV promoter) of GFP cDNA plus 
            miRE-embedded scrambled control shRNA, Blasticidin selection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8772)
  TITLE     Karreth lab plasmids
REFERENCE   2  (bases 1 to 8772)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pLenti-CMV-GFP-miRE_shSCR-Blast
FEATURES             Location/Qualifiers
     source          1..8772
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        23..127
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(128..196,197..988)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(346..365)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1159..1747
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1648..1667
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1901..1918
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2035..2056
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2071..2076,2077..2094,2095..2101)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2109..2125
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2114..2136
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2133..2149
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2133..2149
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     2168..2188
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        2170..2188
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        2216..2442
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             2443..2623
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    2670..2795
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    3288..3521
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             3706..3750
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    4017..4133
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        4156..4459
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4460..4663
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4614..4634
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     4660..4684
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      4753..4762
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(4759..4761,4762..4764,4765..5478)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(4759..4761,4762..4764,4765..5475)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4804..4825)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5065..5084)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5412..5433
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     ncRNA           5662..5676
                     /label=miR-30a loop
                     /note="loop from the the 71-nt precursor of the human 
                     miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     misc_feature    5839..6427
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5892..5912)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6310..6321)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        6440..6939
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(6475..6498)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     6872..6891
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             6968..7366
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     LTR             7509..7742
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7814..7935
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7851..7870)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7905..7924
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      7975..8110
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(8130..8149)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8131..8149)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8159..8176)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8159..8175)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8168..8190)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      8317..8745
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8404..8423)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8614..8635
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc
       61 aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag
      121 gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg
      181 ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt
      241 gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt
      301 tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt
      361 attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa
      421 tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag
      481 agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac
      541 aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac
      601 tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac
      661 cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac
      721 tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact
      781 tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg
      841 tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt
      901 tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat
      961 aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta
     1021 gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa
     1081 tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga
     1141 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac
     1201 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt
     1261 tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc
     1321 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat
     1381 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag
     1441 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc
     1501 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag
     1561 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac
     1621 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg
     1681 gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct
     1741 atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc
     1801 tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga
     1861 gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga
     1921 agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg
     1981 cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt
     2041 gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt
     2101 gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgacc atgattacgc
     2161 caagcgcgca attaaccctc actaaaggga acaaaagctg gagctgcaag cttaatgtag
     2221 tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca acatgcctta
     2281 caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta cgatcgtgcc
     2341 ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga attgccgcat
     2401 tgcagagata ttgtatttaa gtgcctagct cgatacataa acgggtctct ctggttagac
     2461 cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa
     2521 agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag
     2581 agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc ccgaacaggg
     2641 acttgaaagc gaaagggaaa ccagaggagc tctctcgacg caggactcgg cttgctgaag
     2701 cgcgcacggc aagaggcgag gggcggcgac tggtgagtac gccaaaaatt ttgactagcg
     2761 gaggctagaa ggagagagat gggtgcgaga gcgtcagtat taagcggggg agaattagat
     2821 cgcgatggga aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa ttaaaacata
     2881 tagtatgggc aagcagggag ctagaacgat tcgcagttaa tcctggcctg ttagaaacat
     2941 cagaaggctg tagacaaata ctgggacagc tacaaccatc ccttcagaca ggatcagaag
     3001 aacttagatc attatataat acagtagcaa ccctctattg tgtgcatcaa aggatagaga
     3061 taaaagacac caaggaagct ttagacaaga tagaggaaga gcaaaacaaa agtaagacca
     3121 ccgcacagca agcggccgct gatcttcaga cctggaggag gagatatgag ggacaattgg
     3181 agaagtgaat tatataaata taaagtagta aaaattgaac cattaggagt agcacccacc
     3241 aaggcaaaga gaagagtggt gcagagagaa aaaagagcag tgggaatagg agctttgttc
     3301 cttgggttct tgggagcagc aggaagcact atgggcgcag cgtcaatgac gctgacggta
     3361 caggccagac aattattgtc tggtatagtg cagcagcaga acaatttgct gagggctatt
     3421 gaggcgcaac agcatctgtt gcaactcaca gtctggggca tcaagcagct ccaggcaaga
     3481 atcctggctg tggaaagata cctaaaggat caacagctcc tggggatttg gggttgctct
     3541 ggaaaactca tttgcaccac tgctgtgcct tggaatgcta gttggagtaa taaatctctg
     3601 gaacagattt ggaatcacac gacctggatg gagtgggaca gagaaattaa caattacaca
     3661 agcttaatac actccttaat tgaagaatcg caaaaccagc aagaaaagaa tgaacaagaa
     3721 ttattggaat tagataaatg ggcaagtttg tggaattggt ttaacataac aaattggctg
     3781 tggtatataa aattattcat aatgatagta ggaggcttgg taggtttaag aatagttttt
     3841 gctgtacttt ctatagtgaa tagagttagg cagggatatt caccattatc gtttcagacc
     3901 cacctcccaa ccccgagggg acccgacagg cccgaaggaa tagaagaaga aggtggagag
     3961 agagacagag acagatccat tcgattagtg aacggatctc gacggtatcg gttaactttt
     4021 aaaagaaaag gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca
     4081 acagacatac aaactaaaga attacaaaaa caaattacaa aattcaaaat tttatcgata
     4141 agcttgggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa
     4201 cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac
     4261 tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca
     4321 agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg
     4381 gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt
     4441 agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg
     4501 gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg
     4561 gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat
     4621 gggcggtagg cgtgtacggt gggaggtcta tataagcaga gctcgtttag tgaaccgtca
     4681 gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagacacc gactctagag
     4741 gatccaccgg tcgccaccat ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc
     4801 atcctggtcg agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc
     4861 gagggcgatg ccacctacgg caagctgacc ctgaagttca tctgcaccac cggcaagctg
     4921 cccgtgccct ggcccaccct cgtgaccacc ctgacctacg gcgtgcagtg cttcagccgc
     4981 taccccgacc acatgaagca gcacgacttc ttcaagtccg ccatgcccga aggctacgtc
     5041 caggagcgca ccatcttctt caaggacgac ggcaactaca agacccgcgc cgaggtgaag
     5101 ttcgagggcg acaccctggt gaaccgcatc gagctgaagg gcatcgactt caaggaggac
     5161 ggcaacatcc tggggcacaa gctggagtac aactacaaca gccacaacgt ctatatcatg
     5221 gccgacaagc agaagaacgg catcaaggtg aacttcaaga tccgccacaa catcgaggac
     5281 ggcagcgtgc agctcgccga ccactaccag cagaacaccc ccatcggcga cggccccgtg
     5341 ctgctgcccg acaaccacta cctgagcacc cagtccgccc tgagcaaaga ccccaacgag
     5401 aagcgcgatc acatggtcct gctggagttc gtgaccgccg ccgggatcac tctcggcatg
     5461 gacgagctgt acaagtaatg acgtacggcg gccgcctcga ctagggataa cagggtaatt
     5521 gtttgaatga ggcttcagta ctttacagaa tcgttgcctg cacatcttgg aaacacttgc
     5581 tgggattact tcgacttctt aacccaacag aaggctcgag tgctgttgac agtgagcgaa
     5641 gagcagatcg ttcttcaagt agtgaagcca cagatgtatc ttgaagaacg atctgctctg
     5701 tgcctactgc ctcggagaat tcgagcaatt atcttgttta ctaaaactga ataccttgct
     5761 atctctttga tacattttta caaagctgaa ttaaaatggt ataaattaaa tcactttttt
     5821 caattgacgc gtgtcgacaa tcaacctctg gattacaaaa tttgtgaaag attgactggt
     5881 attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat
     5941 catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg
     6001 tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt
     6061 gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact
     6121 ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc
     6181 tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaagctgacg
     6241 tcctttccat ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc
     6301 tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg
     6361 cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc
     6421 tccccgcctg gattctaccg ggtaggggag gcgcttttcc caaggcagtc tggagcatgc
     6481 gctttagcag ccccgctggg cacttggcgc tacacaagtg gcctctggcc tcgcacacat
     6541 tccacatcca ccggtaggcg ccaaccggct ccgttctttg gtggcccctt cgcgccacct
     6601 tctactcctc ccctagtcag gaagttcccc cccgccccgc agctcgcgtc gtgcaggacg
     6661 tgacaaatgg aagtagcacg tctcactagt ctcgtgcaga tggacagcac cgctgagcaa
     6721 tggaagcggg taggcctttg gggcagcggc caatagcagc tttgctcctt cgctttctgg
     6781 gctcagaggc tgggaagggg tgggtccggg ggcgggctca ggggcgggct caggggcggg
     6841 gcgggcgccc gaaggtcctc cggaggcccg gcattctgca cgcttcaaaa gcgcacgtct
     6901 gccgcgctgt tctcctcttc ctcatctccg ggcctttcga cctgcagccc aagcttaccg
     6961 ctagcccatg gccaagcctt tgtctcaaga agaatccacc ctcattgaaa gagcaacggc
     7021 tacaatcaac agcatcccca tctctgaaga ctacagcgtc gccagcgcag ctctctctag
     7081 cgacggccgc atcttcactg gtgtcaatgt atatcatttt actgggggac cttgtgcaga
     7141 actcgtggtg ctgggcactg ctgctgctgc ggcagctggc aacctgactt gtatcgtcgc
     7201 gatcggaaat gagaacaggg gcatcttgag cccctgcgga cggtgccgac aggtgcttct
     7261 cgatctgcat cctgggatca aagccatagt gaaggacagt gatggacagc cgacggcagt
     7321 tgggattcgt gaattgctgc cctctggtta tgtgtgggag ggctaagcta gccgcccgcc
     7381 ccacgacccg cagcgcccga ccgaaaggag cgcacgaccc catgcattcg agggcccggt
     7441 acctttaaga ccaatgactt acaaggcagc tgtagatctt agccactttt taaaagaaaa
     7501 ggggggactg gaagggctag ctcactccca acgaagacaa gatctgcttt ttgcttgtac
     7561 tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc
     7621 actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt
     7681 gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag
     7741 cagtagtagt tcatgtcatc ttattattca gtatttataa cttgcaaaga aatgaatatc
     7801 agagagtgag aggaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat
     7861 cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact
     7921 catcaatgta tcttatcatg tctggctcta gctatcccgc ccctaactcc gcccatcccg
     7981 cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt
     8041 tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt
     8101 tttggaggcc tagggacgta cccaattcgc cctatagtga gtcgtattac gcgcgctcac
     8161 tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc
     8221 ttgcagcaca tccccctttc gccagctggc gtaatagcga agaggcccgc accgatcgcc
     8281 cttcccaaca gttgcgcagc ctgaatggcg aatgggacgc gccctgtagc ggcgcattaa
     8341 gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc
     8401 ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag
     8461 ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca
     8521 aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc
     8581 gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa
     8641 cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct
     8701 attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa
     8761 cgcttacaat tt
//
