LOCUS       sequence-32772-e         811 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 811)
  AUTHORS   Xu W, Lipscombe D
  TITLE     Neuronal Ca(V)1.3alpha(1) L-type channels activate at relatively 
            hyperpolarized membrane potentials and are incompletely inhibited by
            dihydropyridines.
  JOURNAL   J Neurosci. 2001 Aug 15. 21(16):5944-51.
  PUBMED    11487617
REFERENCE   2  (bases 1 to 811)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Neurosci.
            2001 Aug 15. 21(16):5944-51."
COMMENT     Sequence Label: CaV1.3e[8a,11,31b,Delta-32,Delta-42a] mut
FEATURES             Location/Qualifiers
     source          1..811
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(3..20)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(30..71)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 tcatggtgat ggtgatgatg accggtacgc gtagaatcga gaccgaggag agggttaggg
       61 ataggcttac cttcgaaccg cgggattgct acaaggtggt gatgcaaatc atttcatctg
      121 ccaggtcctc ttcctctcgc ccagggtctg gttcctcgtc actgtagcca ggaccaaagt
      181 cctggagctc atagtcctgg cggtgtgaga tggggcccat gtccccgttg gctcgaggac
      241 acacgcttcc attgagcagg gtgctggccg cgctctccat ctcgtctatg gttaggtcac
      301 aggcatcggc gatctcatgt tttgttgctg aggcaaattt tgggtccctt gcgtagcgtc
      361 ccaagccttc ggatatcagg actgcttcca ccaggctgtc tgcactcctc tgcttgtcgc
      421 tgtttttgtt ccggaagctg ctggggacgg tcaggctggc cggtgtgaaa gtcctatagg
      481 agatgtcggg ttcatctgta taccaggaac tgcggtgtag cgacggcagg ctgccattga
      541 cctggtccat agactctgag cgatccacct ggatcaaagg ggtgtagcat ggggtccagt
      601 ctcggtacgg aggggttgct ggaggggtgg cccatgaccg ggtagagtgg ctcggcgagt
      661 acttctgggc tttactggaa tctaggcctg caacggccat gatctgctgc tgcataaggt
      721 gcaggggcag ggcagcgcgg tgaggcaggg caggcgacgg aaggacgtca tcttggctgt
      781 tttgcctgcg cagacactca aagttgaagg a
//
