LOCUS       sequence-327737-        7847 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses a truncation of Hook3 in bacteria for purification.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7847)
  AUTHORS   Schroeder CM, Vale RD
  TITLE     Assembly and activation of dynein-dynactin by the cargo adaptor 
            protein Hook3.
  JOURNAL   J Cell Biol. 2016 Aug 1;214(3):309-18. doi: 10.1083/jcb.201604002.
  PUBMED    27482052
REFERENCE   2  (bases 1 to 7847)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1083/jcb"; 
            journalName: "J Cell Biol"; date: "2016-08-1- 1"; volume: "214"; 
            issue: "3"; pages: "309-18"
COMMENT     Sequence Label: pET28a-Hook3 aa 1-552
FEATURES             Location/Qualifiers
     source          1..7847
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1..20
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1..19
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    20..44
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             59..81
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             99..116
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             126..143
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             147..179
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             join(195..218,219..254,255..278)
                     /codon_start=1
                     /product="two Strep-Tag(R)II moieties connected by a linker
                     for enhanced binding to Strep-Tactin(R), an engineered form
                     of streptavidin (Schmidt et al., 2013)"
                     /label=Twin-Strep-tag
                     /translation="WSHPQFEKGGGSGGGSGGSAWSHPQFEK"
     CDS             join(285..287,288..998)
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /note="mammalian codon-optimized"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     CDS             285..998
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Nager et al., 2011)"
                     /label=superfolder GFP
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     primer_bind     complement(315..343)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     938..958
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     CDS             1005..1025
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             2712..2729
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(2782..2800)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      2796..2843
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      2880..3335
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2967..2986)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3177..3198
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(3427..4242)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(3427..4242)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     4150..4169
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     4234..4253
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      4364..4952
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4853..4872
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5106..5123
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    5138..5277
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(5203..5222)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5363..5385
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(5379..5570)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     CDS             complement(6379..7461)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     7423..7442
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(7462..7539)
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     7745..7764
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 taatacgact cactataggg gaattgtgag cggataacaa ttcccctcta gaaataattt
       61 tgtttaactt taagaaggag atatacatgg gcagcagcca tcatcatcat catcacagca
      121 gcggcctggt gccgcgcggc agccatatgg ctagcatgac tggtggacag caaatgggtc
      181 gcggatccga attctggagt catccacaat ttgagaaggg cggcggcagt ggcggcggca
      241 gtggcggcag tgcctggagc cacccgcagt tcgaaaaagg cagtatgagc aaaggagaag
      301 aacttttcac tggagttgtc ccaattcttg ttgaattaga tggtgatgtt aatgggcaca
      361 aattttctgt ccgtggagag ggtgaaggtg atgctacaaa cggaaaactc acccttaaat
      421 ttatttgcac tactggaaaa ctacctgttc cgtggccaac acttgtcact actctgacct
      481 atggtgttca atgcttttcc cgttatccgg atcacatgaa acggcatgac tttttcaaga
      541 gtgccatgcc cgaaggttat gtacaggaac gcactatatc tttcaaagat gacgggacct
      601 acaagacgcg tgctgaagtc aagtttgaag gtgataccct tgttaatcgt atcgagttaa
      661 agggtattga ttttaaagaa gatggaaaca ttcttggaca caaactcgaa tacaacttta
      721 actcacacaa tgtatacatc acggcagaca aacaaaagaa tggaatcaaa gctaacttca
      781 aaattcgcca caacgttgaa gatggttccg ttcaactagc agaccattat caacaaaata
      841 ctccaattgg cgatggccct gtccttttac cagacaacca ttacctgtcg acacaatctg
      901 tcctttcgaa agatcccaac gaaaagcgtg accacatggt ccttcttgag tttgtaactg
      961 ctgctgggat tacacatggc atggatgagc tctacaaagg atccgaaaat ctgtattttc
     1021 aggggggtgg aggtggcatg ttcagcgtag agtcgctgga gcgggcggag ctgtgcgaga
     1081 gcctcctcac ttggatccag acatttaatg tggatgcacc atgccagacc gtggaagatt
     1141 taacgaatgg ggttgtgatg gcccaggttc ttcaaaagat agatcctgca tattttgatg
     1201 aaaattggct aaacagaatc aaaactgaag taggagataa ttggaggcta aagataagca
     1261 atttaaagaa aattttaaaa ggaatcttgg attataatca tgagatttta ggacagcaaa
     1321 ttaatgactt tacccttcct gatgtgaacc ttattgggga gcattctgat gcagcagagc
     1381 ttggaaggat gcttcagctc atcttaggct gtgctgtgaa ctgtgaacag aagcaagagt
     1441 acatccaagc cattatgatg atggaggaat ctgttcaaca tgttgtcatg acagccattc
     1501 aagagctgat gagtaaagaa tctcctgtct ctgctggaaa tgatgcctat gttgaccttg
     1561 atcgtcagct gaagaaaact acagaggaac taaatgaagc tttgtcagca aaggaagaaa
     1621 ttgctcaaag atgccatgaa ctggatatgc aggttgcagc attgcaggaa gagaaaagta
     1681 gtttgttggc agagaatcag gtattaatgg aaagactcaa tcaatctgat tctatagaag
     1741 accctaacag tccagcagga agaaggcatt tgcagctcca gactcaatta gaacagctcc
     1801 aagaagaaac attcagacta gaagcagcca aagatgatta tcgaatacgt tgtgaagagt
     1861 tagaaaagga gatctctgaa cttcggcaac agaatgatga actgaccact ttggcagatg
     1921 aagctcagtc tctgaaagat gagatcgacg tgctgagaca ttcttctgat aaagtatcta
     1981 aactagaagg tcaagtagaa tcttataaaa agaagctaga agaccttggt gatttaaggc
     2041 ggcaggttaa actcttagaa gagaagaata ccatgtatat gcagaatact gtcagtctag
     2101 aggaagagtt aagaaaggcc aacgcagcgc gaagtcaact tgaaacctac aagagacagg
     2161 tagtagaact acaaaacaga ttatccgaag aatcaaagaa agcagataaa ctagattttg
     2221 aatataagcg gctaaaagaa aaagttgaca gtcttcaaaa agaaaaggac aggctgagaa
     2281 cagaaaggga ttctctgaag gaaaccattg aagagcttcg ttgtgtacaa gctcaagaag
     2341 ggcagctcac aacacaaggg ttaatgcctc ttggaagtca ggagtcttca gacagtctag
     2401 ctgcagagat tgttacacct gaaatcaggg agaaacttat tcgtcttcag catgagaata
     2461 agatgttaaa gcttaaccaa gaaggttcgg acaatgaaaa aatagcctta ttgcagagcc
     2521 ttctagatga tgcaaatcta cgcaagaatg aactggagac agagaatagg ctggtgaatc
     2581 aaagacttct ggaagtacag tcacaagttg aagaattaca aaaatcttta caggatcaag
     2641 gctcaaaagc agaagattca gtccttctaa aaaagaagct tgaagaacat ctataagcgg
     2701 ccgcactcga gcaccaccac caccaccact gagatccggc tgctaacaaa gcccgaaagg
     2761 aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta
     2821 aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggattg gcgaatggga
     2881 cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc
     2941 tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac
     3001 gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag
     3061 tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc
     3121 atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg
     3181 actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata
     3241 agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa
     3301 cgcgaatttt aacaaaatat taacgcttac aatttaggtg gcacttttcg gggaaatgtg
     3361 cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaat
     3421 taattcttag aaaaactcat cgagcatcaa atgaaactgc aatttattca tatcaggatt
     3481 atcaatacca tatttttgaa aaagccgttt ctgtaatgaa ggagaaaact caccgaggca
     3541 gttccatagg atggcaagat cctggtatcg gtctgcgatt ccgactcgtc caacatcaat
     3601 acaacctatt aatttcccct cgtcaaaaat aaggttatca agtgagaaat caccatgagt
     3661 gacgactgaa tccggtgaga atggcaaaag tttatgcatt tctttccaga cttgttcaac
     3721 aggccagcca ttacgctcgt catcaaaatc actcgcatca accaaaccgt tattcattcg
     3781 tgattgcgcc tgagcgagac gaaatacgcg atcgctgtta aaaggacaat tacaaacagg
     3841 aatcgaatgc aaccggcgca ggaacactgc cagcgcatca acaatatttt cacctgaatc
     3901 aggatattct tctaatacct ggaatgctgt tttcccgggg atcgcagtgg tgagtaacca
     3961 tgcatcatca ggagtacgga taaaatgctt gatggtcgga agaggcataa attccgtcag
     4021 ccagtttagt ctgaccatct catctgtaac atcattggca acgctacctt tgccatgttt
     4081 cagaaacaac tctggcgcat cgggcttccc atacaatcga tagattgtcg cacctgattg
     4141 cccgacatta tcgcgagccc atttataccc atataaatca gcatccatgt tggaatttaa
     4201 tcgcggccta gagcaagacg tttcccgttg aatatggctc ataacacccc ttgtattact
     4261 gtttatgtaa gcagacagtt ttattgttca tgaccaaaat cccttaacgt gagttttcgt
     4321 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc
     4381 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc
     4441 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac
     4501 caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac
     4561 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt
     4621 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct
     4681 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat
     4741 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt
     4801 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg
     4861 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt
     4921 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt
     4981 tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg
     5041 tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg
     5101 agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta
     5161 cgcatctgtg cggtatttca caccgcaatg gtgcactctc agtacaatct gctctgatgc
     5221 cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc
     5281 cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct
     5341 tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca
     5401 ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag
     5461 atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg
     5521 cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc
     5581 gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc
     5641 acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa
     5701 ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc
     5761 gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg
     5821 aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg
     5881 aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt
     5941 cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg
     6001 gtcctcaacg acaggagcac gatcatgcgc acccgtgggg ccgccatgcc ggcgataatg
     6061 gcctgcttct cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg
     6121 tgcaagattc cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg
     6181 tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag
     6241 aagacagtca taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact
     6301 gggttgaagg ctctcaaggg catcggtcga gatcccggtg cctaatgagt gagctaactt
     6361 acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg
     6421 cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ccagggtggt
     6481 ttttcttttc accagtgaga cgggcaacag ctgattgccc ttcaccgcct ggccctgaga
     6541 gagttgcagc aagcggtcca cgctggtttg ccccagcagg cgaaaatcct gtttgatggt
     6601 ggttaacggc gggatataac atgagctgtc ttcggtatcg tcgtatccca ctaccgagat
     6661 atccgcacca acgcgcagcc cggactcggt aatggcgcgc attgcgccca gcgccatctg
     6721 atcgttggca accagcatcg cagtgggaac gatgccctca ttcagcattt gcatggtttg
     6781 ttgaaaaccg gacatggcac tccagtcgcc ttcccgttcc gctatcggct gaatttgatt
     6841 gcgagtgaga tatttatgcc agccagccag acgcagacgc gccgagacag aacttaatgg
     6901 gcccgctaac agcgcgattt gctggtgacc caatgcgacc agatgctcca cgcccagtcg
     6961 cgtaccgtct tcatgggaga aaataatact gttgatgggt gtctggtcag agacatcaag
     7021 aaataacgcc ggaacattag tgcaggcagc ttccacagca atggcatcct ggtcatccag
     7081 cggatagtta atgatcagcc cactgacgcg ttgcgcgaga agattgtgca ccgccgcttt
     7141 acaggcttcg acgccgcttc gttctaccat cgacaccacc acgctggcac ccagttgatc
     7201 ggcgcgagat ttaatcgccg cgacaatttg cgacggcgcg tgcagggcca gactggaggt
     7261 ggcaacgcca atcagcaacg actgtttgcc cgccagttgt tgtgccacgc ggttgggaat
     7321 gtaattcagc tccgccatcg ccgcttccac tttttcccgc gttttcgcag aaacgtggct
     7381 ggcctggttc accacgcggg aaacggtctg ataagagaca ccggcatact ctgcgacatc
     7441 gtataacgtt actggtttca cattcaccac cctgaattga ctctcttccg ggcgctatca
     7501 tgccataccg cgaaaggttt tgcgccattc gatggtgtcc gggatctcga cgctctccct
     7561 tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg
     7621 ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg
     7681 ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc
     7741 catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg
     7801 ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaat
//
