LOCUS       sequence-32774-e         950 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 950)
  AUTHORS   Trujillo RD, Yue SB, Tang Y, O'Gorman WE, Chen CZ
  TITLE     The potential functions of primary microRNAs in target recognition 
            and repression.
  JOURNAL   EMBO J. 2010 Oct 6;29(19):3272-85. doi: 10.1038/emboj.2010.208. Epub
            2010 Aug 31.
  PUBMED    20808284
REFERENCE   2  (bases 1 to 950)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/emboj.2010.208"; journalName: "EMBO J"; date: "2010-10-6- 
            6"; volume: "29"; issue: "19"; pages: "3272-85"
COMMENT     Sequence Label: MDH1-pre-let-7hairpin-PGK-GFP
FEATURES             Location/Qualifiers
     source          1..950
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(220..310)
                     /label=H1 promoter
                     /note="human H1 RNA promoter"
     primer_bind     complement(280..299)
                     /label=H1
                     /note="Human H1 promoter, forward primer"
ORIGIN
        1 tacaagtaat gaattaatta agaattgcgg ccgcgtcgac ctgcagccaa gcttatcgat
       61 aaaataaaag attttattta gtctccagaa aaagggggga atgaaagacc ccacctgtag
      121 gtttggcaag aattcttaat taaaaaggta aggtagaaaa ttgcatagtt caccggtggt
      181 aatattccaa actatacaac ctactacctc agggctcgag tggtctcata cagaacttat
      241 aagattccca aatccaaaga catttcacgt ttatggtgat ttcccagaac acatagcgac
      301 atgcaaatat tgggatccgc tagcttaagt aacgccattt tgcaaggcat ggaaaataca
      361 taactgagaa tagagaagtt cagatcaagg ttaggaacag agagacagca gaatatgggc
      421 caaacaggat atctgtggta agcagttcct gccccggctc agggccaaga acagatggtc
      481 cccagatgcg gtcccgccct cagcagtttc tagagaacca tcagatgttt ccagggtgcc
      541 ccaaggacct gaaatgaccc tgtgccttat ttgaactaac caatcagttc gcttctcgct
      601 tctgttcgcg cgcttctgct ccccgagctc aataaaagag cccacaaccc ctcactcggc
      661 gcgccagtcc tccgatagac tgcgtcgccc gggtacccgt gtatccaata aaccctcttg
      721 cagttgcatc cgacttgtgg tctcgctgtt ccttgggagg gtctcctctg agtgattgac
      781 tacccgtcag cgggggtctt tcatgggtaa cagtttcttg aagttggaga acaacattct
      841 gagggtagga gtcgaatatt aagtaatcct gactcaatta gccactgttt tgaatccaca
      901 tactccaata ctcctgaaat agttcattat ggacagcgca naaagagctg
//
