LOCUS       sequence-327748-        3549 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Bacterial expression plasmid for His6-ERK1C202.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3549)
  AUTHORS   Lai KY, Galan SRG, Zeng Y, Zhou TH, He C, Raj R, Riedl J, Liu S, 
            Chooi KP, Garg N, Zeng M, Jones LH, Hutchings GJ, Mohammed S, Nair 
            SK, Chen J, Davis BG, van der Donk WA
  TITLE     LanCLs add glutathione to dehydroamino acids generated at 
            phosphorylated sites in the proteome.
  JOURNAL   Cell. 2021 Apr 27. pii: S0092-8674(21)00436-0. doi: 
            10.1016/j.cell.2021.04.001.
  PUBMED    33932340
REFERENCE   2  (bases 1 to 3549)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2021 
            Apr 27. pii: S0092-8674(21)00436-0. doi: 
            10.1016/j.cell.2021.04.001."
COMMENT     Sequence Label: ERK1C202
FEATURES             Location/Qualifiers
     source          1..3549
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     19..38
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        19..37
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             51..59
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             72..89
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(join(1347..2138,2139..2207))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1970..1989
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2208..2312)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2380..2398
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     rep_origin      2527..3115
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3016..3035
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3269..3286
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     3495..3517
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
ORIGIN
        1 cagctggctt atcgaaatta atacgactca ctatagggag accggaattc taaggaggtt
       61 taaccatggc acatcaccat caccatcata tggcggcggc ggcggctcag gggggcgggg
      121 gcggggagcc ccgtagaacc gagggggtcg gcccgggggt cccgggggag gtggagatgg
      181 tgaaggggca gccgttcgac gtgggcccgc gctacacgca gttgcagtac atcggcgagg
      241 gcgcgtacgg catggtcagc tcggcctatg accacgtgcg caagactcgc gtggccatcc
      301 gtaagatcag ccccttcgaa catcagacct acagccagcg cacgctccgg gagatccaga
      361 tcctgctgcg cttccgccat gagaatgtca tcggcatccg agacattctg cgggcgtcca
      421 ccctggaagc catgagagat gtctacattg tgcaggacct gatggagact gacctgtaca
      481 agttgctgaa aagccagcag ctgagcaatg accatatcag ctacttcctc taccagatcc
      541 tgcggggcct caagtacatc cactccgcca acgtgctcca ccgagatcta aagccctcca
      601 acctgctcat caacaccacc agcgacctta agattagtga tttcggcctg gcccggattg
      661 ccgatcctga gcatgaccac accggcttcc tgtgcgagta tgtggctacg cgctggtacc
      721 gggccccaga gatcatgctg aactccaagg gctataccaa gtccatcgac atctggtctg
      781 tgggctgcat tctggctgag atgctctcta accggcccat cttccctggc aagcactacc
      841 tggatcagct caaccacatt ctgggcatcc tgggctcccc atcccaggag gacctgaata
      901 gtatcatcaa catgaaggcc cgaaactacc tacagtctct gccctccaag accaaggtgg
      961 cttgggccaa gcttttcccc aagtcagact ccaaagccct tgacctgctg gaccggatgt
     1021 taacctttaa ccccaataaa cggatcacag tggaggaagc gctggctcac ccctacctgg
     1081 agcagtacta tgacccgacg gatgagccag tggccgagga gcccttcacc ttcgccatgg
     1141 agctggatga cctacctaag gagcggctga aggagctcat cttccaggag acagcacgct
     1201 tccagcccgg agtgctggag gccccctagt gcatgcggcc gcggatcctc tagagtcgac
     1261 ctgcagccca agcttatcga tgataagctg tcaaacatga gaattaaatc aatctaaagt
     1321 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca
     1381 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg
     1441 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca
     1501 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt
     1561 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt
     1621 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca
     1681 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca
     1741 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga
     1801 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact
     1861 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga
     1921 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caacacggga taataccgcg
     1981 ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc
     2041 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga
     2101 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat
     2161 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt
     2221 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt
     2281 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac
     2341 gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat cacgaggccc
     2401 tttcgtcttc aagaataaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa
     2461 aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg
     2521 atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc
     2581 gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac
     2641 tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca
     2701 ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt
     2761 ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc
     2821 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg
     2881 aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc
     2941 cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac
     3001 gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct
     3061 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc
     3121 cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt
     3181 tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac
     3241 cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg
     3301 cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatggtgcac
     3361 tctcagtaca atctgctctg atgcgctacg tgactgggtc atggctgcgc cccgacaccc
     3421 gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca
     3481 agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg
     3541 cgcgaggcc
//
