LOCUS       sequence-327802-       13439 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Integration Vector, assembled with pHIE047 (TUPV1), pJM466 (TUPV2), 
            pJM452 (TUPV3), pPD613.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13439)
  AUTHORS   Donahue PS, Draut JW, Muldoon JJ, Edelstein HI, Bagheri N, Leonard 
            JN
  TITLE     The COMET toolkit for composing customizable genetic programs in 
            mammalian cells.
  JOURNAL   Nat Commun. 2020 Feb 7;11(1):779. doi: 10.1038/s41467-019-14147-5.
  PUBMED    32034124
REFERENCE   2  (bases 1 to 13439)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2020-02-7"; volume: "11(1):779. doi"; pages: " 
            10.1038/s41467-019-14147-5"
COMMENT     Sequence Label: pHIE055 ZF43x3-S mKate2 EF1a VP16-ZF43 EF1a
            EB...
FEATURES             Location/Qualifiers
     source          1..13439
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1031..1068
                     /label=Bxb1 attB
                     /bound_moiety="Mycobacteriophage Bxb1 integrase"
                     /note="Minimal attB site for the Mycopbacteriophage Bxb1 
                     integrase (Voutev and Mann, 2017)"
     CDS             1079..1678
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(1079..1098)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     1575..1595
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     complement(1791..1810)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    1856..1911
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(1910..1929)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     regulatory      3129..3138
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3135..3833
                     /codon_start=1
                     /product="monomeric far-red fluorescent protein (Shcherbo 
                     et al., 2009)"
                     /label=mKate2
                     /note="mammalian codon-optimized"
                     /translation="MVSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMRIK
                     AVEGGPLPFAFDILATSFMYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLT
                     ATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEASTETLYPADGGLEGRADMAL
                     KLVGGGHLICNLKTTYRSKKPAKNLKMPGVYYVDRRLERIKEADKETYVEQHEVAVARY
                     CDLPSKLGHR"
     polyA_signal    3884..4108
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     promoter        5157..6339
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          5388..6330
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     regulatory      6440..6449
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             6449..6514
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             6521..6541
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    7138..7362
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     promoter        8411..9593
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          8642..9584
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     regulatory      9694..9703
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(9700..9702,9703..9705,9706..10416)
                     /codon_start=1
                     /product="enhanced blue variant of GFP (Ai et al., 2007)"
                     /label=EBFP2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTL
                     KFICTTGKLPVPWPTLVTTLSHGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GTYKTRAEVKFEGDTLVNRIELKGVDFKEDGNILGHKLEYNFNSHNIYIMAVKQKNGIK
                     VNFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDSHYLSTQSVLSKDPNEKRDHMVLL
                     EFRTAAGITLGMDELYK"
     primer_bind     complement(9745..9766)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     CDS             10444..10500
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             10513..10911
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     polyA_signal    10962..11186
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     CDS             complement(12045..12854)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     12768..12787
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     12846..12865
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      complement(join(13013..13439,1..162))
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(13093..13112)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
       61 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
      121 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
      181 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
      241 ttatcaaaaa ggatcttcac ctagatcctt tctcgagctt tcggagtgag atggagtggc
      301 cactgtggaa ctgggcctag catcgcgata agctctagag ggacagcccc cccccaaagc
      361 ccccagggat gtaattacgt ccctcccccg ctagggggca gcagcgagcc gcccggggct
      421 ccgctccggt ccggcgctcc ccccgcatcc ccgagccggc agcgtgcggg gacagcccgg
      481 gcacggggaa ggtggcacgg gatcgctttc ctctgaacgc ttctcgctgc tctttgagcc
      541 tgcagacacc tggggggata cggggaaaaa gctttaggct gaaagagaga tttagaatga
      601 cagaatcata gaacggcctg cgtacggatg gggctctagc tctagagctt gatatcgaat
      661 tcctgcagcc ccgctagagg gacagccccc ccccaaagcc cccagggatg taattacgtc
      721 cctcccccgc tagggggcag cagcgagccg cccggggctc cgctccggtc cggcgctccc
      781 cccgcatccc cgagccggca gcgtgcgggg acagcccggg cacggggaag gtggcacggg
      841 atcgctttcc tctgaacgct tctcgctgct ctttgagcct gcagacacct ggggggatac
      901 ggggaaaaag ctttaggctg aaagagagat ttagaatgac agaatcatag aacggcctgg
      961 gcgcgcctgg tggcgaacga tcaacagacg ggagatctac tattgggaca gcagagatcc
     1021 agttggtaat ggcttgtcga cgacggcggt ctccgtcgtc aggatcatag gtgccaccat
     1081 gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac gacgtccccc gggccgtacg
     1141 caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg acccggaccg
     1201 ccacatcgag cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg ggctcgacat
     1261 cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg gtctggacca cgccggagag
     1321 cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc atggccgagt tgagcggttc
     1381 ccggctggcc gcgcagcaac agatggaagg cctcctggcg ccgcaccggc ccaaggagcc
     1441 cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac cagggcaagg gtctgggcag
     1501 cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg ccttcctgga
     1561 gacatccgcg ccccgcaacc tccccttcta cgagcggctc ggcttcaccg tcaccgccga
     1621 cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg gtgcctgagc
     1681 ttccactgga ttgtacaatt accaactgaa ttcactcctc aggtgcaggc tgcctatcag
     1741 aaggtggtgg ctggtgtggc caatgccctg gctcacaaat accactgaga tctttttccc
     1801 tctgccaaaa attatgggga catcatgaag ccccttgagc atctgacttc tggctaataa
     1861 aggaaattta ttttcattgc aatagtgtgt tggaattttt tgtgtctctc actcggaagg
     1921 acatatggga gggcaaatca tttaaaacat cagaatgagt atttggttta gagtttggca
     1981 acatatgccc atatgctggc tgccatgaac aaaggttggc tataaagagg tcatcagtat
     2041 atgaaacagc cccctgctgt ccattcctta ttccatagaa aagccttgac ttgaggttag
     2101 atttttttta tattttgttt tgtgttattt ttttctttaa catccctaaa attttcctta
     2161 catgttttac tagccagatt tttcctcctc tcctgactac tcccagtcat agctgtccct
     2221 cttctcttat ggagatccgg gcctagcatc gcgataagct ctagagggac agcccccccc
     2281 caaagccccc agggatgtaa ttacgtccct cccccgctag ggggcagcag cgagccgccc
     2341 ggggctccgc tccggtccgg cgctcccccc gcatccccga gccggcagcg tgcggggaca
     2401 gcccgggcac ggggaaggtg gcacgggatc gctttcctct gaacgcttct cgctgctctt
     2461 tgagcctgca gacacctggg gggatacggg gaaaaagctt taggctgaaa gagagattta
     2521 gaatgacaga atcatagaac ggcctgcgta cggatggggc tctagctcta gagcttgata
     2581 tcgaattcct gcagccccgc tagagggaca gccccccccc aaagccccca gggatgtaat
     2641 tacgtccctc ccccgctagg gggcagcagc gagccgcccg gggctccgct ccggtccggc
     2701 gctccccccg catccccgag ccggcagcgt gcggggacag cccgggcacg gggaaggtgg
     2761 cacgggatcg ctttcctctg aacgcttctc gctgctcttt gagcctgcag acacctgggg
     2821 ggatacgggg aaaaagcttt aggctgaaag agagatttag aatgacagaa tcatagaacg
     2881 gcctgggcgc gcctggtggc gaacgatcaa cagacgggag attgccaagg tcttgacaaa
     2941 cgagtgctag tacaagatcg cgtcgacgga tcgggagatc tgaattagag tgaggacaat
     3001 cgtatacaag tagatccaga gtgaggacta cctatagaag taagagtgag gacaatcgta
     3061 tacaagtaga tccctctaga gggtatataa tgggggccac tagtctacta ccagagctca
     3121 tcgctagcgc caccatggtg agcgagctga ttaaggagaa catgcacatg aagctgtata
     3181 tggagggcac cgtgaacaac caccacttca agtgtacctc cgagggcgaa ggcaagccct
     3241 acgagggcac ccagaccatg agaatcaagg cggtcgaggg cggccctctc cccttcgcct
     3301 tcgacatcct ggctaccagc ttcatgtacg gcagcaaaac cttcatcaac cacacccagg
     3361 gcatccccga cttctttaag cagtccttcc ccgagggctt cacatgggag agagtcacca
     3421 catacgaaga tgggggcgtg ctgaccgcta cccaggacac cagcctccag gacggctgcc
     3481 tcatctacaa cgtcaagatc agaggggtga acttcccatc caacggccct gtgatgcaga
     3541 agaaaacact cggctgggag gcctccaccg aaaccctgta ccccgctgac ggcggcctgg
     3601 aaggcagagc cgacatggcc ctgaagctcg tgggcggggg ccacctgatc tgcaacttga
     3661 aaaccacata cagatccaag aaacccgcta agaacctcaa gatgcccggc gtctactatg
     3721 tggacaggag actggaaaga atcaaggagg ccgacaaaga aacctacgtc gagcagcacg
     3781 aggtggctgt ggccagatac tgcgacctcc ctagcaaact ggggcacaga tgagcggccg
     3841 ctcgagtcta gagggcccgt ttaaacccgc tgatcagcct cgactgtgcc ttctagttgc
     3901 cagccatctg ttgtttgccc ctcccccgtg ccttccttga ccctggaagg tgccactccc
     3961 actgtccttt cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct
     4021 attctggggg gtggggtggg gcaggacagc aagggggagg attgggaagt caatagcagg
     4081 catgctgggg atgcggtggg ctctatggct tctgaggcag gtcgagggta catgcgcctt
     4141 actccttgtg agcgcatcgc gataagctct agagggacag ccccccccca aagcccccag
     4201 ggatgtaatt acgtccctcc cccgctaggg ggcagcagcg agccgcccgg ggctccgctc
     4261 cggtccggcg ctccccccgc atccccgagc cggcagcgtg cggggacagc ccgggcacgg
     4321 ggaaggtggc acgggatcgc tttcctctga acgcttctcg ctgctctttg agcctgcaga
     4381 cacctggggg gatacgggga aaaagcttta ggctgaaaga gagatttaga atgacagaat
     4441 catagaacgg cctgcgtacg gatggggctc tagctctaga gcttgatatc gaattcctgc
     4501 agccccgcta gagggacagc ccccccccaa agcccccagg gatgtaatta cgtccctccc
     4561 ccgctagggg gcagcagcga gccgcccggg gctccgctcc ggtccggcgc tccccccgca
     4621 tccccgagcc ggcagcgtgc ggggacagcc cgggcacggg gaaggtggca cgggatcgct
     4681 ttcctctgaa cgcttctcgc tgctctttga gcctgcagac acctgggggg atacggggaa
     4741 aaagctttag gctgaaagag agatttagaa tgacagaatc atagaacggc ctgggcgcgc
     4801 ctggtggcga acgatcaaca gacgggagag ggcaaaaccg atgttgacgg actaatcctg
     4861 acaagatctg gggaggtcgc tgagtagtgc gcgagcaaaa tttaagctac aacaaggcaa
     4921 ggcttgaccg acaattgcat gaagaatctg cttagggtta ggcgttttgc gctgcttcgc
     4981 gatgtacggg ccagatatac gcgtgagtaa ttcatacaaa aggactcgcc cctgccttgg
     5041 ggaatcccag ggaccgtcgt taaactccca ctaacgtaga acccagagat cgctgcgttc
     5101 ccgccccctc acccgcccgc tctcgtcatc actgaggtgg agaagagcat gcgtgaggct
     5161 ccggtgcccg tcagtgggca gagcgcacat cgcccacagt ccccgagaag ttggggggag
     5221 gggtcggcaa ttgaaccggt gcctagagaa ggtggcgcgg ggtaaactgg gaaagtgatg
     5281 tcgtgtactg gctccgcctt tttcccgagg gtgggggaga accgtatata agtgcagtag
     5341 tcgccgtgaa cgttcttttt cgcaacgggt ttgccgccag aacacaggta agtgccgtgt
     5401 gtggttcccg cgggcctggc ctctttacgg gttatggccc ttgcgtgcct tgaattactt
     5461 ccacgcccct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
     5521 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
     5581 cttgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
     5641 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
     5701 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
     5761 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
     5821 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
     5881 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
     5941 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
     6001 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
     6061 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
     6121 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     6181 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     6241 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     6301 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag agctcgttta gtgaaccgtc
     6361 agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagagac cgggaccgat
     6421 ccaaattctc gaggctagcg ccaccatgga ttataaggat cacgatggcg actacaagga
     6481 ccacgatatt gactacaaag acgatgacga taaaatggct cctaagaaaa agcgcaaagt
     6541 cggtatccat ggcgttcccg tcgacggtgg actgcaggcc ccaccaaccg atgtttcttt
     6601 aggtgatgag ttacatttgg acggtgagga cgtagctatg gcacatgcag atgccttaga
     6661 cgattttgac ttggatatgt taggagatgg agattcacca ggacctggtt tcacaccaca
     6721 cgattcagct ccatacggtg ctttggacat ggccgatttc gaatttgaac agatgtttac
     6781 tgatgccttg ggtatagacg aatacggtgg tggagctaga cccggggagc gccccttcca
     6841 gtgtcgcatt tgcatgcgga acttttcgcg ccaggacagg cttgacaggc atacccgtac
     6901 tcataccggt gaaaaaccgt ttcagtgtcg gatctgtatg cgaaatttct cccagaagga
     6961 gcacttggcg gggcatctac gtacgcacac cggcgagaag ccattccaat gccgaatatg
     7021 catgcgcaac ttcagtcgcc gcgacaacct gaaccggcac ctaaaaaccc acctgagagg
     7081 atcttaagcg gccgctcgag tctagagggc ccgtttaaac ccgctgatca gcctcgactg
     7141 tgccttctag ttgccagcca tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg
     7201 aaggtgccac tcccactgtc ctttcctaat aaaatgagga aattgcatcg cattgtctga
     7261 gtaggtgtca ttctattctg gggggtgggg tggggcagga cagcaagggg gaggattggg
     7321 aagtcaatag caggcatgct ggggatgcgg tgggctctat ggcttctgag gcaggtcgaa
     7381 gaactatttc ctggctgtta cgcgagcgca tcgcgataag ctctagaggg acagcccccc
     7441 cccaaagccc ccagggatgt aattacgtcc ctcccccgct agggggcagc agcgagccgc
     7501 ccggggctcc gctccggtcc ggcgctcccc ccgcatcccc gagccggcag cgtgcgggga
     7561 cagcccgggc acggggaagg tggcacggga tcgctttcct ctgaacgctt ctcgctgctc
     7621 tttgagcctg cagacacctg gggggatacg gggaaaaagc tttaggctga aagagagatt
     7681 tagaatgaca gaatcataga acggcctgcg tacggatggg gctctagctc tagagcttga
     7741 tatcgaattc ctgcagcccc gctagaggga cagccccccc ccaaagcccc cagggatgta
     7801 attacgtccc tcccccgcta gggggcagca gcgagccgcc cggggctccg ctccggtccg
     7861 gcgctccccc cgcatccccg agccggcagc gtgcggggac agcccgggca cggggaaggt
     7921 ggcacgggat cgctttcctc tgaacgcttc tcgctgctct ttgagcctgc agacacctgg
     7981 ggggatacgg ggaaaaagct ttaggctgaa agagagattt agaatgacag aatcatagaa
     8041 cggcctgggc gcgcctggtg gcgaacgatc aacagacggg agaggactaa atagtagttc
     8101 agacgccgtt aagcgcaaga tctggggagg tcgctgagta gtgcgcgagc aaaatttaag
     8161 ctacaacaag gcaaggcttg accgacaatt gcatgaagaa tctgcttagg gttaggcgtt
     8221 ttgcgctgct tcgcgatgta cgggccagat atacgcgtga gtaattcata caaaaggact
     8281 cgcccctgcc ttggggaatc ccagggaccg tcgttaaact cccactaacg tagaacccag
     8341 agatcgctgc gttcccgccc cctcacccgc ccgctctcgt catcactgag gtggagaaga
     8401 gcatgcgtga ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga
     8461 gaagttgggg ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa
     8521 ctgggaaagt gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta
     8581 tataagtgca gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca
     8641 ggtaagtgcc gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt
     8701 gccttgaatt acttccacgc ccctggctgc agtacgtgat tcttgatccc gagcttcggg
     8761 ttggaagtgg gtgggagagt tcgaggcctt gcgcttaagg agccccttcg cctcgtgctt
     8821 gagttgaggc ctggcttggg cgctggggcc gccgcgtgcg aatctggtgg caccttcgcg
     8881 cctgtctcgc tgctttcgat aagtctctag ccatttaaaa tttttgatga cctgctgcga
     8941 cgcttttttt ctggcaagat agtcttgtaa atgcgggcca agatctgcac actggtattt
     9001 cggtttttgg ggccgcgggc ggcgacgggg cccgtgcgtc ccagcgcaca tgttcggcga
     9061 ggcggggcct gcgagcgcgg ccaccgagaa tcggacgggg gtagtctcaa gctggccggc
     9121 ctgctctggt gcctggcctc gcgccgccgt gtatcgcccc gccctgggcg gcaaggctgg
     9181 cccggtcggc accagttgcg tgagcggaaa gatggccgct tcccggccct gctgcaggga
     9241 gctcaaaatg gaggacgcgg cgctcgggag agcgggcggg tgagtcaccc acacaaagga
     9301 aaagggcctt tccgtcctca gccgtcgctt catgtgactc cacggagtac cgggcgccgt
     9361 ccaggcacct cgattagttc tcgagctttt ggagtacgtc gtctttaggt tggggggagg
     9421 ggttttatgc gatggagttt ccccacactg agtgggtgga gactgaagtt aggccagctt
     9481 ggcacttgat gtaattctcc ttggaatttg ccctttttga gtttggatct tggttcattc
     9541 tcaagcctca gacagtggtt caaagttttt ttcttccatt tcaggtgtcg tgagagctcg
     9601 tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc tccatagaag
     9661 agaacgggac cgatccaaat tctcgaggct agcgccacca tggtgagcaa gggcgaggag
     9721 ctgttcaccg gggtggtgcc catcctggtc gagctggacg gcgacgtaaa cggccacaag
     9781 ttcagcgtga ggggcgaggg cgagggcgat gccaccaacg gcaagctgac cctgaagttc
     9841 atctgcacca ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac cctgagccac
     9901 ggcgtgcagt gcttcgcccg ctaccccgac cacatgaagc agcacgactt cttcaagtcc
     9961 gccatgcccg aaggctacgt ccaggagcgc accatcttct tcaaggacga cggcacctac
    10021 aagacccgcg ccgaggtgaa gttcgagggc gacaccctag tgaaccgcat cgagctgaag
    10081 ggcgtcgact tcaaggagga cggcaacatc ctggggcaca agctggagta caacttcaac
    10141 agccacaaca tctatatcat ggccgtcaag cagaagaacg gcatcaaggt gaacttcaag
    10201 atccgccaca acgtggagga cggcagcgtg cagctcgccg accactacca gcagaacacc
    10261 cccatcggcg acggccccgt gctgctgccc gacagccact acctgagcac ccagtccgtg
    10321 ctgagcaaag accccaacga gaagcgcgat cacatggtcc tgctggagtt ccgcaccgcc
    10381 gccgggatca ctctcggcat ggacgagctg tacaaggaat tcggtagcaa gcttggtagt
    10441 ggagcaacta acttttccct gctcaaacaa gccggggatg tagaggagaa ccccggcccc
    10501 gcccccctta agatggccaa gcctttgtct caagaagaat ccaccctcat tgaaagagca
    10561 acggctacaa tcaacagcat ccccatctct gaggactaca gcgtcgccag cgcagctctc
    10621 tctagcgacg gccgcatctt cactggtgtc aatgtatatc attttactgg gggaccttgt
    10681 gcagaactcg tggtgctggg cactgctgct gctgcggcag ctggcaacct gacttgtatc
    10741 gtcgcgatcg gaaatgagaa caggggcatc ttgagcccct gcggacggtg ccgacaggtg
    10801 cttctcgatc tgcatcctgg gatcaaagcc atagtgaagg acagtgatgg acagccgacg
    10861 gcagttggga ttcgtgaatt gctgccctct ggttatgtgt gggagggcta agcggccgct
    10921 cgagtctaga gggcccgttt aaacccgctg atcagcctcg actgtgcctt ctagttgcca
    10981 gccatctgtt gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg ccactcccac
    11041 tgtcctttcc taataaaatg aggaaattgc atcgcattgt ctgagtaggt gtcattctat
    11101 tctggggggt ggggtggggc aggacagcaa gggggaggat tgggaagtca atagcaggca
    11161 tgctggggat gcggtgggct ctatggcttc tgaggcaggt cgaagagact gcccgacaca
    11221 tcttagtgag cgcatcgcga taagctctag agggacagcc cccccccaaa gcccccaggg
    11281 atgtaattac gtccctcccc cgctaggggg cagcagcgag ccgcccgggg ctccgctccg
    11341 gtccggcgct ccccccgcat ccccgagccg gcagcgtgcg gggacagccc gggcacgggg
    11401 aaggtggcac gggatcgctt tcctctgaac gcttctcgct gctctttgag cctgcagaca
    11461 cctgggggga tacggggaaa aagctttagg ctgaaagaga gatttagaat gacagaatca
    11521 tagaacggcc tgcgtacgga tggggctcta gctctagagc ttgatatcga attcctgcag
    11581 ccccgctaga gggacagccc ccccccaaag cccccaggga tgtaattacg tccctccccc
    11641 gctagggggc agcagcgagc cgcccggggc tccgctccgg tccggcgctc cccccgcatc
    11701 cccgagccgg cagcgtgcgg ggacagcccg ggcacgggga aggtggcacg ggatcgcttt
    11761 cctctgaacg cttctcgctg ctctttgagc ctgcagacac ctggggggat acggggaaaa
    11821 agctttaggc tgaaagagag atttagaatg acagaatcat agaacggcct gggcgcgcct
    11881 ggtggcgaac gatcaacaga cgggagaggt tacgaggatg cacatgtgac cgagggatgg
    11941 aacgacgcgt aactcacgtt aagggatttt ggtcatgagc ttgcgccgtc ccgtcaagtc
    12001 agcgtaatgc tctgccagtg ttacaaccaa ttaaccaatt ctgattagaa aaactcatcg
    12061 agcatcaaat gaaactgcaa tttattcata tcaggattat caataccata tttttgaaaa
    12121 agccgtttct gtaatgaagg agaaaactca ccgaggcagt tccataggat ggcaagatcc
    12181 tggtatcggt ctgcgattcc gactcgtcca acatcaatac aacctattaa tttcccctcg
    12241 tcaaaaataa ggttatcaag tgagaaatca ccatgagtga cgactgaatc cggtgagaat
    12301 ggcaaaagtt tatgcatttc tttccagact tgttcaacag gccagccatt acgctcgtca
    12361 tcaaaatcac tcgcatcaac caaaccgtta ttcattcgtg attgcgcctg agcgagacga
    12421 aatacgcgat cgctgttaaa aggacaatta caaacaggaa tcgaatgcaa ccggcgcagg
    12481 aacactgcca gcgcatcaac aatattttca cctgaatcag gatattcttc taatacctgg
    12541 aatgctgttt ttccggggat cgcagtggtg agtaaccatg catcatcagg agtacggata
    12601 aaatgcttga tggtcggaag aggcataaat tccgtcagcc agtttagtct gaccatctca
    12661 tctgtaacat cattggcaac gctacctttg ccatgtttca gaaacaactc tggcgcatcg
    12721 ggcttcccat acaagcgata gattgtcgca cctgattgcc cgacattatc gcgagcccat
    12781 ttatacccat ataaatcagc atccatgttg gaatttaatc gcggcctcga cgtttcccgt
    12841 tgaatatggc tcataacacc ccttgtatta ctgtttatgt aagcagacag ttttattgtt
    12901 catgatgata tatttttatc ttgtgcaatg taacatcaga gattttgaga cacgggccat
    12961 gctagctcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
    13021 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
    13081 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
    13141 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
    13201 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
    13261 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
    13321 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
    13381 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactac
//
