LOCUS       sequence-327804-        9829 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Human FTSJ3 retroviral mammalian expression vector. FLAG/Gateway 
            modified pQCXIP vector with C-terminal FLAG epitope and bicistronic 
            puromycin expression..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9829)
  AUTHORS   Li S, Wang L, Fu B, Berman MA, Diallo A, Dorf ME
  TITLE     TRIM65 regulates microRNA activity by ubiquitination of TNRC6.
  JOURNAL   Proc Natl Acad Sci U S A. 2014 May 13;111(19):6970-5. doi: 
            10.1073/pnas.1322545111. Epub 2014 Apr 28.
  PUBMED    24778252
REFERENCE   2  (bases 1 to 9829)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1322545111"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2014-05-13- 13"; volume: "111"; issue: "19"; pages: "6970-5"
COMMENT     Sequence Label: pEXPqcxip-hFTSJ3-FLAG
FEATURES             Location/Qualifiers
     source          1..9829
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        309..512
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             513..688
                     /label=5' LTR (truncated)
                     /note="truncated long terminal repeat from Moloney murine 
                     sarcoma virus"
     misc_feature    751..950
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             1151..1567
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1497..1519
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1535..1551
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     enhancer        1604..1907
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1908..2111
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2108..2132
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    2299..2323
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     RBS             2979..2987
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     protein_bind    complement(4874..4898)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             4900..4923
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     misc_feature    join(4956..5530,5531..5533,5534..5542)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5123..5140)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5350..5369
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             5562..6161
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5562..5581)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6058..6078
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             6535..6960
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     Moloney murine leukemia virus"
     primer_bind     complement(7063..7085)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(7221..7241)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7226..7555
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7406..7541
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7577..7596
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(7677..7694)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7848..8436)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7928..7947)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8607..9398,9399..9467))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9230..9249
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9468..9572)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     9640..9658
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     enhancer        join(9758..9829,1..308)
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc
       61 cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac
      121 gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata
      181 tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc
      241 agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta
      301 ttaccatggt gatgcggttt tggcagtaca tcaatgggcg tggatagcgg tttgactcac
      361 ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttgg caccaaaatc
      421 aacgggactt tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc
      481 gtgtacggtg ggaggtctat ataagcagag ctcaataaaa gagcccacaa cccctcactc
      541 ggcgcgccag tcttccgata gactgcgtcg cccgggtacc cgtattccca ataaagcctc
      601 ttgctgtttg catccgaatc gtggtctcgc tgttccttgg gagggtctcc tctgagtgat
      661 tgactaccca cgacgggggt ctttcatttg ggggctcgtc cgggatttgg agacccctgc
      721 ccagggacca ccgacccacc accgggaggt aagctggcca gcaacttatc tgtgtctgtc
      781 cgattgtcta gtgtctatgt ttgatgttat gcgcctgcgt ctgtactagt tagctaacta
      841 gctctgtatc tggcggaccc gtggtggaac tgacgagttc tgaacacccg gccgcaaccc
      901 tgggagacgt cccagggact ttgggggccg tttttgtggc ccgacctgag gaagggagtc
      961 gatgtggaat ccgaccccgt caggatatgt ggttctggta ggagacgaga acctaaaaca
     1021 gttcccgcct ccgtctgaat ttttgctttc ggtttggaac cgaagccgcg cgtcttgtct
     1081 gctgcagcgc tgcagcatcg ttctgtgttg tctctgtctg actgtgtttc tgtatttgtc
     1141 tgaaaattag ggccagactg ttaccactcc cttaagtttg accttaggtc actggaaaga
     1201 tgtcgagcgg atcgctcaca accagtcggt agatgtcaag aagagacgtt gggttacctt
     1261 ctgctctgca gaatggccaa cctttaacgt cggatggccg cgagacggca cctttaaccg
     1321 agacctcatc acccaggtta agatcaaggt cttttcacct ggcccgcatg gacacccaga
     1381 ccaggtcccc tacatcgtga cctgggaagc cttggctttt gacccccctc cctgggtcaa
     1441 gccctttgta caccctaagc ctccgcctcc tcttcctcca tccgccccgt ctctccccct
     1501 tgaacctcct cgttcgaccc cgcctcgatc ctccctttat ccagccctca ctccttctct
     1561 aggcgccgga attgaagatc tgggggatcg atcctctaga gtccgttaca taacttacgg
     1621 taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt
     1681 atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac
     1741 ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg
     1801 acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact
     1861 ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt
     1921 ggcagtacat caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc
     1981 ccattgacgt caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc
     2041 gtaacaactc cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata
     2101 taagcagagc tcgtttagtg aaccgtcaga tcgcctggag acgccatcca cgctgttttg
     2161 acctccatag aagacaccgg gaccgatcca gcctccgcgg ccgggaacgg tgcattggaa
     2221 cggacctgca ggaattgatc cgcggccgca ccggtaggcc tcgtacgctt aattaacgga
     2281 tcggagcggc cgctcttcac aagtttgtac aaaaaagcag gctccaccat gggcaagaag
     2341 ggcaaagttg gcaagagccg acgagacaag ttttatcact tggcgaagga gacgggttac
     2401 cgttcccgat ctgctttcaa gctgatccag ctcaatcgcc gctttcagtt cctgcagaaa
     2461 gcccgagcct tgctggacct gtgtgctgcg ccagggggat ggctgcaggt agctgccaag
     2521 tttatgcctg tatccagcct tattgtggga gtggacctgg ttccaatcaa gcctctcccc
     2581 aatgtggtga ctctccagga ggacatcaca acagaacgtt gtaggcaggc cctgaggaag
     2641 gagctgaaga cctggaaggt tgatgttgtg ctcaatgatg gggcccccaa cgttggggct
     2701 agctgggtcc atgatgctta ctcacaagcc catttgacac tgatggctct acgtttggct
     2761 tgtgactttt tggcccgtgg tggcagcttc atcacaaagg ttttccgttc tcgtgactat
     2821 cagcctctgc tatggatctt tcagcagctg ttccgccgtg tccaggccac caagccccaa
     2881 gcctctcgcc atgaatctgc agagatcttt gtagtctgcc aaggattcct ggcccctgac
     2941 aaggttgaca gtaaattctt tgaccccaaa tttgccttta aggaggttga agttcaggct
     3001 aagaccgtta ctgaattggt tactaagaag aagccaaagg ctgaaggcta tgctgagggt
     3061 gacctcactc tctatcaccg tacctcagtc actgacttcc tccgagctgc caaccctgtt
     3121 gacttcctct ccaaggccag cgaaatcatg gtagatgatg aagagttggc acagcatcca
     3181 gctaccactg aggacatacg ggtgtgctgt caggacatca gagtgttggg gcgcaaggag
     3241 ctcaggtcgc tactaaactg gagaacaaaa cttcggcgat atgtggccaa gaagctgaaa
     3301 gaacaagcaa aggcactgga catcagcctc agctctggag aggaagatga aggtgatgag
     3361 gaggactcaa cagctggaac cacaaagcag ccctctaagg aggaggagga agaggaggag
     3421 gaggaacaac tgaaccagac cttggcagaa atgaaggccc aggaggtggc ggaattgaag
     3481 aggaagaaaa agaagctgtt gcgtgagcag agaaagcagc gggagcgtgt ggagctgaag
     3541 atggatctgc ctggggtttc cattgcagac gagggggaga ctggcatgtt ctccttgtgc
     3601 accatccggg gtcaccagtt attagaggaa gtaacacaag gggatatgag tgcagcagac
     3661 acatttctgt ccgatctgcc aagggatgat atctatgtgt cagatgttga ggacgacggt
     3721 gatgacacat ctctggatag tgacctggat ccagaggagc tggcaggagt caggggacat
     3781 cagggtctaa gggaccaaaa gcgtatgcga cttactgaag tgcaagatga taaagaggag
     3841 gaggaggagg agaatccact gctggtacca ctggaggaaa aggcagtact gcaggaagaa
     3901 caagccaacc tgtggttctc aaagggcagc tttgctggga tcgaggacga tgccgatgag
     3961 gccctggaga tcagtcaggc ccagctgtta tttgagaacc ggcggaaggg acggcagcag
     4021 cagcagaagc agcagctgcc acagacaccc ccttcctgtt tgaagactga gataatgtct
     4081 cccctgtacc aagatgaagc ccctaaggga acagaggctt cttcggggac agaagctgcc
     4141 actggccttg aaggggaaga aaaggatggc atctcagaca gtgatagcag tactagcagt
     4201 gaggaagaag agagctggga acccctccgt ggtaagaagc gaagccgtgg gcctaagtca
     4261 gatgatgacg ggtttgagat agtgcctatt gaggacccag cgaaacatcg gatactggac
     4321 cccgaaggcc ttgctctagg tgctgttatt gcctcttcca aaaaggccaa gagagacctc
     4381 atagataact ccttcaaccg gtacacattt aatgaggatg agggggagct tccggagtgg
     4441 tttgtgcaag aggaaaagca gcaccggata cgacagttgc ctgttggtaa gaaggaggtg
     4501 gagcattacc ggaaacgctg gcgggaaatc aatgcacgtc ccatcaagaa ggtggctgag
     4561 gctaaggcta gaaagaaaag gaggatgctg aagaggctgg agcagaccag gaagaaggca
     4621 gaagccgtgg tgaacacagt ggacatctca gaacgagaga aagtggcaca gctgcgaagt
     4681 ctctacaaga aggctgggct tggcaaggag aaacgccatg tcacctacgt tgtagccaaa
     4741 aaaggtgtgg gccgcaaagt gcgccggcca gctggagtca gaggtcattt caaggtggtg
     4801 gactcaagga tgaagaagga ccaaagagca cagcaacgta aggaacaaaa gaaaaaacac
     4861 aaacggaagt tggacccagc tttcttgtac aaagtggtgg attacaagga tgacgacgat
     4921 aagtgattcg aaggatcctc cgatccggaa ttccccccct ctccctcccc cccccctaac
     4981 gttactggcc gaagccgctt ggaataaggc cggtgtgcgt ttgtctatat gttattttcc
     5041 accatattgc cgtcttttgg caatgtgagg gcccggaaac ctggccctgt cttcttgacg
     5101 agcattccta ggggtctttc ccctctcgcc aaaggaatgc aaggtctgtt gaatgtcgtg
     5161 aaggaagcag ttcctctgga agcttcttga agacaaacaa cgtctgtagc gaccctttgc
     5221 aggcagcgga accccccacc tggcgacagg tgcctctgcg gccaaaagcc acgtgtataa
     5281 gatacacctg caaaggcggc acaaccccag tgccacgttg tgagttggat agttgtggaa
     5341 agagtcaaat ggctctcctc aagcgtattc aacaaggggc tgaaggatgc ccagaaggta
     5401 ccccattgta tgggatctga tctggggcct cggtgcacat gctttacatg tgtttagtcg
     5461 aggttaaaaa aacgtctagg ccccccgaac cacggggacg tggttttcct ttgaaaaaca
     5521 cgatgataag cttgccacaa cccacaagga gacgaccttc catgaccgag tacaagccca
     5581 cggtgcgcct cgccacccgc gacgacgtcc cccgggccgt acgcaccctc gccgccgcgt
     5641 tcgccgacta ccccgccacg cgccacaccg tcgacccgga ccgccacatc gagcgggtca
     5701 ccgagctgca agaactcttc ctcacgcgcg tcgggctcga catcggcaag gtgtgggtcg
     5761 cggacgacgg cgccgcggtg gcggtctgga ccacgccgga gagcgtcgaa gcgggggcgg
     5821 tgttcgccga gatcggcccg cgcatggccg agttgagcgg ttcccggctg gccgcgcagc
     5881 aacagatgga aggcctcctg gcgccgcacc ggcccaagga gcccgcgtgg ttcctggcca
     5941 ccgtcggcgt ctcgcccgac caccagggca agggtctggg cagcgccgtc gtgctccccg
     6001 gagtggaggc ggccgagcgc gccggggtgc ccgccttcct ggagacctcc gcgccccgca
     6061 acctcccctt ctacgagcgg ctcggcttca ccgtcaccgc cgacgtcgag gtgcccgaag
     6121 gaccgcgcac ctggtgcatg acccgcaagc ccggtgcctg acgcccgccc cacgacccgc
     6181 agcgcccgac cgaaaggagc gcacgacccc atggctccga ccgaagccac ccggggcggc
     6241 cccgccgacc ccgcacccgc ccccgaggcc caccgactct agtcgagggc tgcagcgctg
     6301 cagaggccga gtgcagaact gctccaaagg gacctcaagg ctttccgagg gacactaggc
     6361 tgactccatc gagccagtgt agagataagc ttatcgatta gtccaatttg ttaaagacag
     6421 gatatcagtg gtccaggctc tagttttgac tcaacaatat caccagctga agcctataga
     6481 gtacgagcca tagataaaat aaaagatttt atttagtctc cagaaaaagg ggggaatgaa
     6541 agaccccacc tgtaggtttg gcaagctagc ttaagtaacg ccattttgca aggcatggaa
     6601 aaatacataa ctgagaatag agaagttcag atcaaggtca ggaacagatg gaacagggtc
     6661 gaccctagag aaccatcaga tgtttccagg gtgccccaag gacctgaaat gaccctgtgc
     6721 cttatttgaa ctaaccaatc agttcgcttc tcgcttctgt tcgcgcgctt ctgctccccg
     6781 agctcaataa aagagcccac aacccctcac tcggggcgcc agtcctccga ttgactgagt
     6841 cgcccgggta cccgtgtatc caataaaccc tcttgcagtt gcatccgact tgtggtctcg
     6901 ctgttccttg ggagggtctc ctctgagtga ttgactaccc gtcagcgggg gtctttcatt
     6961 tgggggctcg tccgggatcg ggagacccct gcccagggac caccgaccca ccaccgggag
     7021 gtaagctggc tgcctcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct
     7081 cccggagacg gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg
     7141 cgcgtcagcg ggtgttggcg ggtgtcgggg cgcagccatg acccagtcac gtagcgatag
     7201 cggagtgtag atccggctgt ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc
     7261 cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca ggtgtggaaa
     7321 gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac
     7381 catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc
     7441 tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc
     7501 tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaaaagct
     7561 tactggctta actatgcggc atcagagcag attgtactga gagtgcacca tatgcggtgt
     7621 gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggcgctcttc cgcttcctcg
     7681 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag
     7741 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa
     7801 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
     7861 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
     7921 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
     7981 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
     8041 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
     8101 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
     8161 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
     8221 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
     8281 actagaagga cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
     8341 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
     8401 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
     8461 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca
     8521 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt
     8581 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca
     8641 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg
     8701 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca
     8761 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt
     8821 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt
     8881 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctgcaggcat cgtggtgtca
     8941 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca
     9001 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga
     9061 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact
     9121 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga
     9181 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caacacggga taataccgcg
     9241 ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc
     9301 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga
     9361 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat
     9421 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt
     9481 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt
     9541 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac
     9601 gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat cacgaggccc
     9661 tttcgtcttc aagaattagc ttggccattg catacgttgt atccatatca taatatgtac
     9721 atttatattg gctcatgtcc aacattaccg ccatgttgac attgattatt gactagttat
     9781 taatagtaat caattacggg gtcattagtt catagcccat atatggagt
//
