LOCUS       sequence-327816-        6590 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Human MBOAT7 mammalian expression vector with 3 C-terminal FLAG 
            tags..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6590)
  AUTHORS   Li S, Wang L, Berman M, Kong YY, Dorf ME
  TITLE     Mapping a dynamic innate immunity protein interaction network 
            regulating type I interferon production.
  JOURNAL   Immunity. 2011 Sep 23;35(3):426-40. Epub 2011 Sep 8.
  PUBMED    21903422
REFERENCE   2  (bases 1 to 6590)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Immunity.";
            date: "2011-09-23"; volume: "35(3)"; pages: "426-40. Epub 2011 Sep 
            8"
COMMENT     Sequence Label: pCMV3Tag8li-hMBOAT7-3xFLAG
FEATURES             Location/Qualifiers
     source          1..6590
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             600..671
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags"
                     /label=3xFLAG
                     /translation="DYKDDDDKDYKDDDDKDYKDDDDK"
     primer_bind     complement(724..743)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(725..743)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(772..789)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    778..985
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      complement(992..1447)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1129..1150)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     1341..1360
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        1474..1578
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1579..1647,1648..2436)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1797..1816)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        2530..2675
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     CDS             2718..3755
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPDRE
                     MGEAN"
     primer_bind     complement(3753..3772)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    3797..3845
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      4396..4984
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4885..4904
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     enhancer        5159..5462
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        5463..5666
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     5617..5637
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     promoter        5712..5730
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 gcccaccccc cagcagtccg gagaaggcgg cttccttgga gtatgactat gagaccatcc
       61 gcaacatcga ctgctacagc acagatttct gcgtgcgggt gcgcgatggc atgcggtact
      121 ggaacatgac ggtgcagtgg tggctggcgc agtatatcta caagagcgca cctgcccgtt
      181 cctatgtcct gcggagcgcc tggaccatgc tgctgagcgc ctactggcac ggcctccacc
      241 cgggctacta cctgagcttc ctgaccatcc cgctgtgcct ggctgccgag ggccggctgg
      301 agtcagccct gcgggggcgg ctgagcccag ggggccagaa ggcctgggac tgggtgcact
      361 ggttcctgaa gatgcgcgcc tatgactaca tgtgcatggg cttcgtgctg ctctccttgg
      421 ccgacaccct tcggtactgg gcctccatct acttctgtat ccacttcctg gccctggcag
      481 ccctggggct ggggctggct ttaggtgggg gcagccccag ccggcggaag gcagcatccc
      541 agcccaccag ccttgccccg gagaagctcc gggaggaggc ggccgcaatc gatgtcgagg
      601 attacaagga tgacgacgat aaggactata aggacgatga tgacaaggac tacaaagatg
      661 atgacgataa atagggcccg gtaccttaat taattaaggt accaggtaag tgtacccaat
      721 tcgccctata gtgagtcgta ttacaattca ctcgatcggc tcgctgatca gcctcgactg
      781 tgccttctag ttgccagcca tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg
      841 aaggtgccac tcccactgtc ctttcctaat aaaatgagga aattgcatcg cattgtctga
      901 gtaggtgtca ttctattctg gggggtgggg tggggcagga cagcaagggg gaggattggg
      961 aagacaatag caggcatgct ggggaacgcg taaattgtaa gcgttaatat tttgttaaaa
     1021 ttcgcgttaa atttttgtta aatcagctca ttttttaacc aataggccga aatcggcaaa
     1081 atcccttata aatcaaaaga atagaccgag atagggttga gtgttgttcc agtttggaac
     1141 aagagtccac tattaaagaa cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag
     1201 ggcgatggcc cactacgtga accatcaccc taatcaagtt ttttggggtc gaggtgccgt
     1261 aaagcactaa atcggaaccc taaagggagc ccccgattta gagcttgacg gggaaagccg
     1321 gcgaacgtgg cgagaaagga agggaagaaa gcgaaaggag cgggcgctag ggcgctggca
     1381 agtgtagcgg tcacgctgcg cgtaaccacc acacccgccg cgcttaatgc gccgctacag
     1441 ggcgcgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc
     1501 taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa
     1561 tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt
     1621 gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct
     1681 gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc
     1741 cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta
     1801 tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac
     1861 tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc
     1921 atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac
     1981 ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg
     2041 gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac
     2101 gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc
     2161 gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt
     2221 gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga
     2281 gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc
     2341 cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag
     2401 atcgctgaga taggtgcctc actgattaag cattggcctc aggattagtg cttcatcccc
     2461 gtggcccgtt gctcgcgttt gctggcggtg tccccggaag aaatatattt gcatgtcttt
     2521 agttctatga tgacacaaac cccgcccagc gtcttgtcat tggcgaattc gaacacgcag
     2581 atgcagtcgg ggcggcgcgg tccgaggtcc acttcgcata ttaaggtgac gcgtgtggcc
     2641 tcgaacaccg agcgaccctg cagcgacccg cttaacagcg tcaacagcgt gccgcagatc
     2701 ccggggggca atgagatatg aaaaagcctg aactcaccgc gacgtctgtc gagaagtttc
     2761 tgatcgaaaa gttcgacagc gtctccgacc tgatgcagct ctcggagggc gaagaatctc
     2821 gtgctttcag cttcgatgta ggagggcgtg gatatgtcct gcgggtaaat agctgcgccg
     2881 atggtttcta caaagatcgt tatgtttatc ggcactttgc atcggccgcg ctcccgattc
     2941 cggaagtgct tgacattggg gaattcagcg agagcctgac ctattgcatc tcccgccgtg
     3001 cacagggtgt cacgttgcaa gacctgcctg aaaccgaact gcccgctgtt ctgcagccgg
     3061 tcgcggaggc catggatgcg atcgctgcgg ccgatcttag ccagacgagc gggttcggcc
     3121 cattcggacc gcaaggaatc ggtcaataca ctacatggcg tgatttcata tgcgcgattg
     3181 ctgatcccca tgtgtatcac tggcaaactg tgatggacga caccgtcagt gcgtccgtcg
     3241 cgcaggctct cgatgagctg atgctttggg ccgaggactg ccccgaagtc cggcacctcg
     3301 tgcacgcgga tttcggctcc aacaatgtcc tgacggacaa tggccgcata acagcggtca
     3361 ttgactggag cgaggcgatg ttcggggatt cccaatacga ggtcgccaac atcttcttct
     3421 ggaggccgtg gttggcttgt atggagcagc agacgcgcta cttcgagcgg aggcatccgg
     3481 agcttgcagg atcgccgcgg ctccgggcgt atatgctccg cattggtctt gaccaactct
     3541 atcagagctt ggttgacggc aatttcgatg atgcagcttg ggcgcagggt cgatgcgacg
     3601 caatcgtccg atccggagcc gggactgtcg ggcgtacaca aatcgcccgc agaagcgcgg
     3661 ccgtctggac cgatggctgt gtagaagtac tcgccgatag tggaaaccga cgccccagca
     3721 ctcgtccgga tcgggagatg ggggaggcta actgaaacac ggaaggagac aataccggaa
     3781 ggaacccgcg ctatgacggc aataaaaaga cagaataaaa cgcacgggtg ttgggtcgtt
     3841 tgttcataaa cgcggggttc ggtcccaggg ctggcactct gtcgataccc caccgagacc
     3901 ccattggggc caatacgccc gcgtttcttc cttttcccca ccccaccccc caagttcggg
     3961 tgaaggccca gggctcgcag ccaacgtcgg ggcggcaggc cctgccatag ccactggccc
     4021 cgtgggttag ggacggggtc ccccatgggg aatggtttat ggttcgtggg ggttattatt
     4081 ttgggcgttg cgtggggtca ggtccacgac tggactgagc agacagaccc atggtttttg
     4141 gatggcctgg gcatggaccg catgtactgg cgcgacacga acaccgggcg tctgtggctg
     4201 ccaaacaccc ccgaccccca aaaaccaccg cgcggatttc tggcgccgcc ggacgaacta
     4261 aacctgacta cgacatctct gccccttctt cgctggtacg aggagcgctt tgcctcaggt
     4321 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa
     4381 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
     4441 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
     4501 gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta
     4561 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
     4621 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
     4681 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
     4741 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
     4801 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
     4861 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
     4921 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
     4981 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
     5041 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg ccatgcatta
     5101 gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg
     5161 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga
     5221 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat
     5281 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa
     5341 gtacgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca
     5401 tgaccttatg ggactttcct acttggcagt acatctacgt attagtcatc gctattacca
     5461 tggtgatgcg gttttggcag tacatcaatg ggcgtggata gcggtttgac tcacggggat
     5521 ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg
     5581 actttccaaa atgtcgtaac aactccgccc cattgacgca aatgggcggt aggcgtgtac
     5641 ggtgggaggt ctatataagc agagctggtt tagtgaaccg tcagatccgc tagcgattac
     5701 gccaagctcg aaattaaccc tcactaaagg gaacaaaagc tggctcgagg ccatgtcgcc
     5761 tgaagaatgg acgtatctag tggttcttct tatctccatc cccatcggct tcctctttaa
     5821 gaaagccggt cctgggctga agagatgggg agcagccgct gtgggcctgg ggctcaccct
     5881 gttcacctgt ggcccccaca ctttgcattc tctggtcacc atcctcggga cctgggccct
     5941 cattcaggcc cagccctgct cctgccacgc cctggctctg gcctggactt tctcctatct
     6001 cctgttcttc cgagccctca gcctcctggg cctgcccact cccacgccct tcaccaatgc
     6061 cgtccagctg ctgctgacgc tgaagctggt gagcctggcc agtgaagtcc aggacctgca
     6121 tctggcccag aggaaggaaa tggcctcagg cttcagcaag gggcccaccc tggggctgct
     6181 gcccgacgtg ccctccctga tggagacact cagctacagc tactgctacg tgggaatcat
     6241 gacaggcccg ttcttccgct accgcaccta cctggactgg ctggagcagc ccttccccgg
     6301 ggcagtgccc agcctgcggc ccctgctgcg ccgcgcctgg ccggccccgc tcttcggcct
     6361 gctgttcctg ctctcctctc acctcttccc gctggaggcc gtgcgcgagg acgccttcta
     6421 cgcccgcccg ctgcccgccc gcctcttcta catgatcccc gtcttcttcg ccttccgcat
     6481 gcgcttctac gtggcctgga ttgccgccga gtgcggctgc attgccgccg gctttggggc
     6541 ctaccccgtg gccgccaaag cccgggccgg aggcggcccc accctccaat
//
