LOCUS       sequence-327827-        6812 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Human TXLNA mammalian expression vector with 3 C-terminal FLAG 
            tags..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6812)
  AUTHORS   Li S, Wang L, Berman M, Kong YY, Dorf ME
  TITLE     Mapping a dynamic innate immunity protein interaction network 
            regulating type I interferon production.
  JOURNAL   Immunity. 2011 Sep 23;35(3):426-40. Epub 2011 Sep 8.
  PUBMED    21903422
REFERENCE   2  (bases 1 to 6812)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Immunity.";
            date: "2011-09-23"; volume: "35(3)"; pages: "426-40. Epub 2011 Sep 
            8"
COMMENT     Sequence Label: pCMV3Tag8li-hTXLNA-3xFLAG
FEATURES             Location/Qualifiers
     source          1..6812
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      16..604
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     505..524
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     enhancer        779..1082
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1083..1286
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1237..1257
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     promoter        1332..1350
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     CDS             3032..3103
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags"
                     /label=3xFLAG
                     /translation="DYKDDDDKDYKDDDDKDYKDDDDK"
     primer_bind     complement(3156..3175)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3157..3175)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3204..3221)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3210..3417
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      complement(3424..3879)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3561..3582)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3773..3792
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        3906..4010
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4011..4079,4080..4868)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4229..4248)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        4962..5107
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     CDS             5150..6187
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPDRE
                     MGEAN"
     primer_bind     complement(6185..6204)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    6229..6277
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
ORIGIN
        1 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
       61 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
      121 gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta
      181 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
      241 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
      301 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
      361 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
      421 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
      481 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
      541 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
      601 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
      661 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg ccatgcatta
      721 gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg
      781 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga
      841 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat
      901 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa
      961 gtacgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca
     1021 tgaccttatg ggactttcct acttggcagt acatctacgt attagtcatc gctattacca
     1081 tggtgatgcg gttttggcag tacatcaatg ggcgtggata gcggtttgac tcacggggat
     1141 ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg
     1201 actttccaaa atgtcgtaac aactccgccc cattgacgca aatgggcggt aggcgtgtac
     1261 ggtgggaggt ctatataagc agagctggtt tagtgaaccg tcagatccgc tagcgattac
     1321 gccaagctcg aaattaaccc tcactaaagg gaacaaaagc tggctcgaga ccatgaagaa
     1381 ccaagacaaa aagaacgggg ctgccaaaca atccaatcca aaaagcagcc caggacaacc
     1441 ggaagcagga cccgagggag cccaggagcg gcccagccag gcggctcctg cagtagaagc
     1501 agaaggtccc ggcagcagcc aggctcctcg gaagccggag ggggctcaag ccagaacggc
     1561 tcagtctggg gcccttcgtg atgtctctga ggagctgagc cgccaactgg aagacatact
     1621 gagcacatac tgtgtggaca ataaccaggg gggccccggc gaggatgggg cacagggtga
     1681 gccggctgaa cccgaagatg cagagaagtc ccggacctat gtggcaagga atggggagcc
     1741 tgaaccaact ccagtagtca atggagagaa ggaaccctcc aagggggatc caaacacaga
     1801 agagatccgg cagagtgacg aggtcggaga ccgagaccat cgaaggccac aggagaagaa
     1861 aaaagccaag ggtttgggga aggagatcac gttgctgatg cagacattga atactctgag
     1921 taccccagag gagaagctgg ctgctctgtg caagaagtat gctgaactgc tggaggagca
     1981 ccggaattca cagaagcaga tgaagctcct acagaaaaag cagagccagc tggtgcaaga
     2041 gaaggaccac ctgtgcggtg agcacagcaa ggccgtcctg gcccgcagca agcttgagag
     2101 cctatgccgt gagctgcagc ggcacaaccg ctccctcaag gaagaaggtg tgcagcgggc
     2161 ccgggaggag gaggagaagc gcaaggaggt gacctcgcac ttccaggtga cactgaatga
     2221 cattcagctg cagatggaac agcacaatga gcgcaactcc aagctgcgcc aagagaacat
     2281 ggagctggct gagaggctca agaagctgat tgagcagtat gagctgcgcg aggagcatat
     2341 cgacaaagtc ttcaaacaca aggacctaca acagcagctg gtggatgcca agctccagca
     2401 ggcccaggag atgctaaagg aggcagaaga gcggcaccag cgggagaagg attttctcct
     2461 gaaagaggca gtagagtccc agaggatgtg tgagctgatg aagcagcaag agacccacct
     2521 gaagcaacag cttgccctat acacagagaa gtttgaggag ttccagaaca cactttccaa
     2581 aagcagcgag gtattcacca cattcaagca ggagatggaa aagatgacta agaagatcaa
     2641 gaagctggag aaagaaacca ccatgtaccg gtcccggtgg gagagcagca acaaggccct
     2701 gcttgagatg gctgaggaga aaacagtccg ggataaagaa ctggagggcc tgcaggtaaa
     2761 aatccaacgg ctggagaagc tgtgccgggc actgcagaca gagcgcaatg acctgaacaa
     2821 gagggtacag gacctgagtg ctggtggcca gggctccctc actgacagtg gccctgagag
     2881 gaggccagag gggcctgggg ctcaagcacc cagctccccc agggtcacag aagcgccttg
     2941 ctacccagga gcaccgagca cagaagcatc aggccagact gggcctcaag agcccacctc
     3001 cgccagggcc gcggccgcaa tcgatgtcga ggattacaag gatgacgacg ataaggacta
     3061 taaggacgat gatgacaagg actacaaaga tgatgacgat aaatagggcc cggtacctta
     3121 attaattaag gtaccaggta agtgtaccca attcgcccta tagtgagtcg tattacaatt
     3181 cactcgatcg gctcgctgat cagcctcgac tgtgccttct agttgccagc catctgttgt
     3241 ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc actcccactg tcctttccta
     3301 ataaaatgag gaaattgcat cgcattgtct gagtaggtgt cattctattc tggggggtgg
     3361 ggtggggcag gacagcaagg gggaggattg ggaagacaat agcaggcatg ctggggaacg
     3421 cgtaaattgt aagcgttaat attttgttaa aattcgcgtt aaatttttgt taaatcagct
     3481 cattttttaa ccaataggcc gaaatcggca aaatccctta taaatcaaaa gaatagaccg
     3541 agatagggtt gagtgttgtt ccagtttgga acaagagtcc actattaaag aacgtggact
     3601 ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg cccactacgt gaaccatcac
     3661 cctaatcaag ttttttgggg tcgaggtgcc gtaaagcact aaatcggaac cctaaaggga
     3721 gcccccgatt tagagcttga cggggaaagc cggcgaacgt ggcgagaaag gaagggaaga
     3781 aagcgaaagg agcgggcgct agggcgctgg caagtgtagc ggtcacgctg cgcgtaacca
     3841 ccacacccgc cgcgcttaat gcgccgctac agggcgcgtc aggtggcact tttcggggaa
     3901 atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca
     3961 tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc
     4021 aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc
     4081 acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt
     4141 acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt
     4201 ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg
     4261 ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact
     4321 caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg
     4381 ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga
     4441 aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg
     4501 aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa
     4561 tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac
     4621 aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc
     4681 cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca
     4741 ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga
     4801 gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta
     4861 agcattggcc tcaggattag tgcttcatcc ccgtggcccg ttgctcgcgt ttgctggcgg
     4921 tgtccccgga agaaatatat ttgcatgtct ttagttctat gatgacacaa accccgccca
     4981 gcgtcttgtc attggcgaat tcgaacacgc agatgcagtc ggggcggcgc ggtccgaggt
     5041 ccacttcgca tattaaggtg acgcgtgtgg cctcgaacac cgagcgaccc tgcagcgacc
     5101 cgcttaacag cgtcaacagc gtgccgcaga tcccgggggg caatgagata tgaaaaagcc
     5161 tgaactcacc gcgacgtctg tcgagaagtt tctgatcgaa aagttcgaca gcgtctccga
     5221 cctgatgcag ctctcggagg gcgaagaatc tcgtgctttc agcttcgatg taggagggcg
     5281 tggatatgtc ctgcgggtaa atagctgcgc cgatggtttc tacaaagatc gttatgttta
     5341 tcggcacttt gcatcggccg cgctcccgat tccggaagtg cttgacattg gggaattcag
     5401 cgagagcctg acctattgca tctcccgccg tgcacagggt gtcacgttgc aagacctgcc
     5461 tgaaaccgaa ctgcccgctg ttctgcagcc ggtcgcggag gccatggatg cgatcgctgc
     5521 ggccgatctt agccagacga gcgggttcgg cccattcgga ccgcaaggaa tcggtcaata
     5581 cactacatgg cgtgatttca tatgcgcgat tgctgatccc catgtgtatc actggcaaac
     5641 tgtgatggac gacaccgtca gtgcgtccgt cgcgcaggct ctcgatgagc tgatgctttg
     5701 ggccgaggac tgccccgaag tccggcacct cgtgcacgcg gatttcggct ccaacaatgt
     5761 cctgacggac aatggccgca taacagcggt cattgactgg agcgaggcga tgttcgggga
     5821 ttcccaatac gaggtcgcca acatcttctt ctggaggccg tggttggctt gtatggagca
     5881 gcagacgcgc tacttcgagc ggaggcatcc ggagcttgca ggatcgccgc ggctccgggc
     5941 gtatatgctc cgcattggtc ttgaccaact ctatcagagc ttggttgacg gcaatttcga
     6001 tgatgcagct tgggcgcagg gtcgatgcga cgcaatcgtc cgatccggag ccgggactgt
     6061 cgggcgtaca caaatcgccc gcagaagcgc ggccgtctgg accgatggct gtgtagaagt
     6121 actcgccgat agtggaaacc gacgccccag cactcgtccg gatcgggaga tgggggaggc
     6181 taactgaaac acggaaggag acaataccgg aaggaacccg cgctatgacg gcaataaaaa
     6241 gacagaataa aacgcacggg tgttgggtcg tttgttcata aacgcggggt tcggtcccag
     6301 ggctggcact ctgtcgatac cccaccgaga ccccattggg gccaatacgc ccgcgtttct
     6361 tccttttccc caccccaccc cccaagttcg ggtgaaggcc cagggctcgc agccaacgtc
     6421 ggggcggcag gccctgccat agccactggc cccgtgggtt agggacgggg tcccccatgg
     6481 ggaatggttt atggttcgtg ggggttatta ttttgggcgt tgcgtggggt caggtccacg
     6541 actggactga gcagacagac ccatggtttt tggatggcct gggcatggac cgcatgtact
     6601 ggcgcgacac gaacaccggg cgtctgtggc tgccaaacac ccccgacccc caaaaaccac
     6661 cgcgcggatt tctggcgccg ccggacgaac taaacctgac tacgacatct ctgccccttc
     6721 ttcgctggta cgaggagcgc tttgcctcag gtcatgacca aaatccctta acgtgagttt
     6781 tcgttccact gagcgtcaga ccccgtagaa aa
//
