LOCUS       sequence-327842-        3549 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Bacterial expression plasmid for His6-ERK1C82C202.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3549)
  AUTHORS   Lai KY, Galan SRG, Zeng Y, Zhou TH, He C, Raj R, Riedl J, Liu S, 
            Chooi KP, Garg N, Zeng M, Jones LH, Hutchings GJ, Mohammed S, Nair 
            SK, Chen J, Davis BG, van der Donk WA
  TITLE     LanCLs add glutathione to dehydroamino acids generated at 
            phosphorylated sites in the proteome.
  JOURNAL   Cell. 2021 Apr 27. pii: S0092-8674(21)00436-0. doi: 
            10.1016/j.cell.2021.04.001.
  PUBMED    33932340
REFERENCE   2  (bases 1 to 3549)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2021 
            Apr 27. pii: S0092-8674(21)00436-0. doi: 
            10.1016/j.cell.2021.04.001."
COMMENT     Sequence Label: ERK1C82C202
FEATURES             Location/Qualifiers
     source          1..3549
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     2..21
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2..20
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             34..42
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             55..72
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(join(1330..2121,2122..2190))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1953..1972
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2191..2295)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2363..2381
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     rep_origin      2510..3098
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2999..3018
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3252..3269
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     3478..3500
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
ORIGIN
        1 ttaatacgac tcactatagg gagaccggaa ttctaaggag gtttaaccat ggcacatcac
       61 catcaccatc atatggcggc ggcggcggct caggggggcg ggggcgggga gccccgtaga
      121 accgaggggg tcggcccggg ggtcccgggg gaggtggaga tggtgaaggg gcagccgttc
      181 gacgtgggcc cgcgctacac gcagttgcag tacatcggcg agggcgcgta cggcatggtc
      241 agctcggcct atgaccacgt gcgcaagact cgcgtggcca tccgtaagat cagccccttc
      301 gaacatcaga cctactgcca gcgcacgctc cgggagatcc agatcctgct gcgcttccgc
      361 catgagaatg tcatcggcat ccgagacatt ctgcgggcgt ccaccctgga agccatgaga
      421 gatgtctaca ttgtgcagga cctgatggag actgacctgt acaagttgct gaaaagccag
      481 cagctgagca atgaccatat cagctacttc ctctaccaga tcctgcgggg cctcaagtac
      541 atccactccg ccaacgtgct ccaccgagat ctaaagccct ccaacctgct catcaacacc
      601 accagcgacc ttaagattag tgatttcggc ctggcccgga ttgccgatcc tgagcatgac
      661 cacaccggct tcctgtgcga gtatgtggct acgcgctggt accgggcccc agagatcatg
      721 ctgaactcca agggctatac caagtccatc gacatctggt ctgtgggctg cattctggct
      781 gagatgctct ctaaccggcc catcttccct ggcaagcact acctggatca gctcaaccac
      841 attctgggca tcctgggctc cccatcccag gaggacctga atagtatcat caacatgaag
      901 gcccgaaact acctacagtc tctgccctcc aagaccaagg tggcttgggc caagcttttc
      961 cccaagtcag actccaaagc ccttgacctg ctggaccgga tgttaacctt taaccccaat
     1021 aaacggatca cagtggagga agcgctggct cacccctacc tggagcagta ctatgacccg
     1081 acggatgagc cagtggccga ggagcccttc accttcgcca tggagctgga tgacctacct
     1141 aaggagcggc tgaaggagct catcttccag gagacagcac gcttccagcc cggagtgctg
     1201 gaggccccct agtgcatgcg gccgcggatc ctctagagtc gacctgcagc ccaagcttat
     1261 cgatgataag ctgtcaaaca tgagaattaa atcaatctaa agtatatatg agtaaacttg
     1321 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
     1381 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
     1441 atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc
     1501 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
     1561 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
     1621 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat
     1681 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
     1741 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
     1801 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
     1861 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
     1921 accgagttgc tcttgcccgg cgtcaacacg ggataatacc gcgccacata gcagaacttt
     1981 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct
     2041 gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac
     2101 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
     2161 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat
     2221 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
     2281 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtctaag aaaccattat
     2341 tatcatgaca ttaacctata aaaataggcg tatcacgagg ccctttcgtc ttcaagaata
     2401 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt
     2461 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
     2521 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
     2581 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
     2641 agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
     2701 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
     2761 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
     2821 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
     2881 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
     2941 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
     3001 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
     3061 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
     3121 tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg
     3181 attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa
     3241 cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc
     3301 tccttacgca tctgtgcggt atttcacacc gcatatggtg cactctcagt acaatctgct
     3361 ctgatgcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg
     3421 cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg
     3481 ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cccagctggc
     3541 ttatcgaaa
//
