LOCUS       sequence-327847-       12909 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses four gRNA targeting BTub gene with 3xp3-eGFP marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12909)
  AUTHORS   Li M, Yang T, Bui M, Gamez S, Wise T, Kandul NP, Liu J, Alcantara L,
            Lee H, Edula JR, Raban R, Zhan Y, Wang Y, DeBeaubien N, Chen J, 
            Sanchez C HM, Bennett JB, Antoshechkin I, Montell C, Marshall JM, 
            Akbari OS
  TITLE     Suppressing mosquito populations with precision guided sterile 
            males.
  JOURNAL   Nat Commun. 2021 Sep 10;12(1):5374. doi: 10.1038/s41467-021-25421-w.
  PUBMED    34508072
REFERENCE   2  (bases 1 to 12909)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2021-09-10"; volume: "12(1):5374. doi"; pages: " 
            10.1038/s41467-021-25421-w"
COMMENT     Sequence Label: OA-1067A1
FEATURES             Location/Qualifiers
     source          1..12909
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             join(1..3,4..6,7..720)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(1..3,4..6,7..717)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(46..67)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(307..326)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     654..675
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    843..964
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(880..899)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     934..953
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     repeat_region   1632..1666
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(1847..1863)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1847..1863)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1860..1882)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1871..1887
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1895..1901,1902..1919,1920..1925))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1940..1961
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2078..2095)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2249..2837)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2329..2348)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3008..3799,3800..3868))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3631..3650
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3869..3973)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     4041..4059
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(4097..4119)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     4219..4238
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4432..4454
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4446..4463
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4447..4463
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     repeat_region   5167..5229
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     regulatory      join(12904..12909,1..4)
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac
       61 ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac
      121 ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc
      181 ctcgtgacca ccctgaccta cggcgtgcag tgcttcagcc gctaccccga ccacatgaag
      241 cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc
      301 ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg
      361 gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac
      421 aagctggagt acaactacaa cagccacaac gtctatatca tggccgacaa gcagaagaac
      481 ggcatcaagg tgaacttcaa gatccgccac aacatcgagg acggcagcgt gcagctcgcc
      541 gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac
      601 tacctgagca cccagtccgc cctgagcaaa gaccccaacg agaagcgcga tcacatggtc
      661 ctgctggagt tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaagtaa
      721 agcggccgcg actctagatc ataatcagcc ataccacatt tgtagaggtt ttacttgctt
      781 taaaaaacct cccacacctc cccctgaacc tgaaacataa aatgaatgca attgttgttg
      841 ttaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca
      901 caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat
      961 cttaaagctt ctaggccggc cgatctcgga tctgacaatg ttcagtgcag agactcggct
     1021 acgcctcgtg gactttgaag ttgaccaaca atgtttattc ttacctctaa tagtcctctg
     1081 tggcaaggtc aagattctgt tagaagccaa tgaagaacct ggttgttcaa taacattttg
     1141 ttcgtctaat atttcactac cgcttgacgt tggctgcact tcatgtacct catctataaa
     1201 cgcttcttct gtatcgctct ggacgtcatc ttcacttacg tgatctgata tttcactgtc
     1261 agaatcctca ccaacaagct cgtcatcgct ttgcagaaga gcagagagga tatgctcatc
     1321 gtctaaagaa ctacccattt tattatatat tagtcacgat atctataaca agaaaatata
     1381 tatataataa gttatcacgt aagtagaaca tgaaataaca atataattat cgtatgagtt
     1441 aaatcttaaa agtcacgtaa aagataatca tgcgtcattt tgactcacgc ggtcgttata
     1501 gttcaaaatc agtgacactt accgcattga caagcacgcc tcacgggagc tccaagcggc
     1561 gactgagatg tcctaaatgc acagcgacgg attcgcgcta tttagaaaga gagagcaata
     1621 tttcaagaat gcatgcgtca attttacgca gactatcttt ctagggttaa aaaagatttg
     1681 cgctttactc gacctaaact ttaaacacgt catagaatct tcgtttgaca aaaaccacat
     1741 tgtggccaag ctgtgtgacg cgacgcgcgc taaagaatgg caaaccaagt cgcgcgagcg
     1801 tcgactctag aggatccccg ggtaccgagc tcgaattcgt aatcatgtca tagctgtttc
     1861 ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt
     1921 gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc
     1981 ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg
     2041 ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct
     2101 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca
     2161 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
     2221 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
     2281 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
     2341 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
     2401 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
     2461 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
     2521 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
     2581 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
     2641 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg
     2701 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
     2761 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
     2821 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
     2881 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga
     2941 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt
     3001 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt
     3061 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat
     3121 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag
     3181 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct
     3241 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt
     3301 tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg
     3361 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca
     3421 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt
     3481 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat
     3541 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac
     3601 cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa
     3661 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt
     3721 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt
     3781 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa
     3841 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt
     3901 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa
     3961 taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtctaagaa accattatta
     4021 tcatgacatt aacctataaa aataggcgta tcacgaggcc ctttcgtctc gcgcgtttcg
     4081 gtgatgacgg tgaaaacctc tgacacatgc agctcccgga gacggtcaca gcttgtctgt
     4141 aagcggatgc cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc
     4201 ggggctggct taactatgcg gcatcagagc agattgtact gagagtgcac catatgcggt
     4261 gtgaaatacc gcacagatgc gtaaggagaa aataccgcat caggcgccat tcgccattca
     4321 ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta cgccagctgg
     4381 cgaaaggggg atgtgctgca aggcgattaa gttgggtaac gccagggttt tcccagtcac
     4441 gacgttgtaa aacgacggcc agtgccaagc tttgtttaaa aatataacaa aattgtgatc
     4501 ccacaaaatg aagtggggca aaatcaaata attaatagtg tccgtaaact tgttggtctt
     4561 caactttttg aggaacacgt tggacggcaa atccgtgact ataacacaag ttgatttaat
     4621 aattttagcc aacacgtcgg gctgcgtgtt ttttgccgac gcgtctgtgt acacgttgat
     4681 taactggtcg attaaactgt tgaaataatt taatttttgg ttcttcttta aatctgtgat
     4741 gaaatttttt aaaataactt taaattcttc attggtaaaa aatgccacgt tttgcaactt
     4801 gtgagggtct aatatgaggt caaactcagt aggagtttta tccaaaaaag aaaacatgat
     4861 tacgtctgta cacgaacgcg tattaacgca gagtgcaaag tataagaggg ttaaaaaata
     4921 tattttacgc accatatacg catcgggttg atatcgttaa tatggatcaa tttgaacagt
     4981 tgattaacgt gtctctgctc aagtctttga tcaaaacgca aatcgacgaa aatgtgtcgg
     5041 acaatatcaa gtcgatgagc gaaaaactaa aaaggctaga atacgacaat ctcacagaca
     5101 gcgttgagat atacggtatt cacgacagca ggctgaataa taaaaaaatt agaaactatt
     5161 atttaaccct agaaagataa tcatattgtg acgtacgtta aagataatca tgcgtaaaat
     5221 tgacgcatgt gttttatcgg tctgtatatc gaggtttatt tattaatttg aatagatatt
     5281 aagttttatt atatttacac ttacatacta ataataaatt caacaaacaa tttatttatg
     5341 tttatttatt tattaaaaaa aacaaaaact caaaatttct tctataaagt aacaaaactt
     5401 ttaaacattc tctcttttac aaaaataaac ttattttgta ctttaaaaac agtcatgttg
     5461 tattataaaa taagtaatta gcttaactta tacataatag aaacaaatta tacttattag
     5521 tcagtcagaa acaactttgg cacatatcaa tattatgctc tcgacaaata acttttttgc
     5581 attttttgca cgatgcattt gcctttcgcc ttattttaga ggggcagtaa gtacagtaag
     5641 tacgtttttt cattactggc tcttcagtac tgtcatctga tgtaccaggc acttcatttg
     5701 gcaaaatatt agagatatta tcgcgcaaat atctcttcaa agtaggagct tctaaacgct
     5761 tacgcataaa cgatgacgtc aggctcatgt aaaggtttct cataaatttt ttgcgacttt
     5821 gaaccttttc tcccttgcta ctgacattat ggctgtatat aataaaagaa tttatgcagg
     5881 caatgtttat cattccgtac aataatgcca taggccacct attcgtcttc ctactgcagg
     5941 tcatcacaga acacatttgg tctagcgtgt ccactccgcc tttagtttga ttataataca
     6001 taaccatttg cggtttaccg gtactttcgt tgatagaagc atcctcatca caagatgata
     6061 ataagtatac catcttagct ggcttcggtt tatatgagac gagagtaagg ggtccgtcaa
     6121 aacaaaacat cgatgttccc actggcctgg agcgactgtt tttcagtact tccggtatct
     6181 cgcgtttgtt tgatcgcacg gttcccacaa tggttaattc gagctcgccc gggctcctag
     6241 gagatcttca tcacaaacga aactattata atcgtcttca ggggaacggt agcatcatat
     6301 ctgtcaccag tcagtcggat tcctttcttt cggcataaat tatgctcggg tgacgagacg
     6361 agttgaaaat cccgtaaatt aaaacatata ccacactcgc cgtcctagac gacgacgacg
     6421 acgacgattc ccaaggaatt gaacccttca tttgcatttc actaccgctc tgagagatgc
     6481 aaagcgcgcc agacagtcga tcgaatctgc agaccagaga gtcacatcaa gaatgttgac
     6541 attccaccag aagactctaa ttattcttcg ctcgactggg aaaactgtta acaactgctc
     6601 gagtgtggtc ggagcggctc acagtgcgtt ttcccgtaga caaaaatcaa atttcatggt
     6661 atttttcggc gataaacagt aagttaaagg gaacccgaat ttagtgctat ataatttaat
     6721 tccactagag tttgtatcct ttgatagata cgcgtatttc gacctcaact gcaaggccgt
     6781 cgtgtactag acttgactaa tccagactgg tcttttagtt atgacttctg tccacatctc
     6841 catacattca acgcactgtg cggctgtgct gtgcgactcc gtcgagtcga ccaacatagt
     6901 tgaaacaaat tgaatattta attgatcgtt ataggaatgg tgttagatga gtcatccttt
     6961 acagtaagca catacagtat tataattgaa gatcgtcggc agataggtgt gtagggtaga
     7021 gtatcagcaa taagttggga cgtttgactt tttgtaggta gacaaaaact aaactttttt
     7081 tcgcttctct atgtgtgccc ctgggtagcg ttccgttccg attggggtgc gaacgaatga
     7141 aatcgcccat cgagttgata cgtccatcca tcgctagaac cgcgttcgct gtagaagact
     7201 atataagagc agaggcaaga gtagtgaaat tactacaacg aggctaccgg gtcttagagc
     7261 tatgctggaa acagcatagc aagttaagat aaggctagtc cgttatcaac ttgaaaaagt
     7321 ggcaccgagt cggtgctttt tttggtggtt gttcatcgat tcctaatatt gcagtgaagc
     7381 agtttgttgt gcatggcgaa gataaggtta gggcgaggaa tgccttggaa ataagcggca
     7441 ctgggccgta tgaataaaaa gcgttgaact ttactacatg tagcatctac tcaaaaacaa
     7501 aatccacaaa gtttactaca cttttggtat gaataaaaac cttttcgaac atgtagtacc
     7561 tactacttta agaaaattct attcaaagta cagagtaatg ctgcatgtta agaaaattcc
     7621 attcagagtg actcttgaaa tcaataaaat catgcatata tgccaaattt gtcacttatt
     7681 cacgtgacaa attatgtgaa aatatgaata ttactacatt cccgctatgc atcgcgtaat
     7741 cgatcatgaa atttatgttg gttatgtaac tttacagtat tccctttatg tgcatgtgaa
     7801 ttttataatg atttgacaac aaggattttc cttagaattt tcgaatttta gctagaaatc
     7861 tgtatcctat gaatgaatct tgagataaca gcaattgtaa cattataaag ttcctaacat
     7921 tttcaagatt ccagtattga gtcgcgatcg cttaattaac cgcggatatg tcttgccgaa
     7981 gcacattaac ccaagccatt tatatatcaa gtacaagttg atttgattct agtgatggac
     8041 tgacacgaat ctatggcact gttgcagagc ttacaaacag gtacccggag tcgcttcaat
     8101 tattcaatca tccattcaaa aacttccatc tcttccaatt agccaatatt caaattggaa
     8161 tccagccagc cgacggaggc agatggtcta cctggcaact atagagtttc catgtttcca
     8221 gactttccct cccggtaaac ggagacaaaa cgacagacgt aagtaggtac atatgcatac
     8281 cgcacggaca aatcaaattt gtctggcagc tccaattaga gtcgttaaaa atttaacgat
     8341 gcgctaaata acttcaagct atttgtctcg ctggattggc ttcgagtggt aagatcctat
     8401 caaatgccga aaacaaaaaa ctttcttctt aattgttcgt tcttcaacac ctctccatgg
     8461 tgataacgga tacggtttca ttgtcagcat ccatcctccg aaaaatacat tacgccttga
     8521 aatatgcaat cgcaaacacg gatctgtttg gaacatttat tttactatga agagatgcga
     8581 taggtaatat ttatttgagc gtttaagata ctcattgttc tctcaaagaa tgtcattgaa
     8641 agccaacgag gtcaaatcaa atattataat aaaaaggtca aagaggacta acttaaagct
     8701 ctctttatgg ataggaaaaa atattttcgc ccatcgctag aacttttacc gtttccattg
     8761 agtatataac taagatgaat gaggctaatt gatgtctccg caatacgccc cgggtcttag
     8821 agctatgctg gaaacagcat agcaagttaa gataaggcta gtccgttatc aacttgaaaa
     8881 agtggcaccg agtcggtgct ttttttaaga tgcttcggca attaataata aggaatggta
     8941 aagcattctt caaatgttac tatgttctca aattttcaag aattaggtat tcaagaaatt
     9001 ctttcaggca tattccataa tctgcacaag cagctacttt agaaattatt gccgggctta
     9061 aattatatgt acaacatttc ctgagtattt tctgacaaaa attctcacaa gggaaggtca
     9121 cgatggagtt acacggggtt tttaaccgga ctatttttgt tagattctac atgtcgaaat
     9181 atgaaaaacc ccaaagcctt tcgggtgatg gaccagtggt gtagccagga ggggctctga
     9241 aaggggtaat tttgtacgta tattatatta ttaagctaat tggaaatttg acaaaaatat
     9301 ttttttagta tttattgtga tggtagagaa caaaattgac attaatgtac gtttgaaatg
     9361 tatttgccat gcctttggcg tactaacatg gagtatggac accaaaaccg atttcgctag
     9421 cgtttaaact catcacaaac gaaactatta taatcgtctt caggggaacg gtagcatcat
     9481 atctgtcacc agtcagtcgg attcctttct ttcggcataa attatgctcg ggtgacgaga
     9541 cgagttgaaa atcccgtaaa ttaaaacata taccacactc gccgtcctag acgacgacga
     9601 cgacgacgat tcccaaggaa ttgaaccctt catttgcatt tcactaccgc tctgagagat
     9661 gcaaagcgcg ccagacagtc gatcgaatct gcagaccaga gagtcacatc aagaatgttg
     9721 acattccacc agaagactct aattattctt cgctcgactg ggaaaactgt taacaactgc
     9781 tcgagtgtgg tcggagcggc tcacagtgcg ttttcccgta gacaaaaatc aaatttcatg
     9841 gtatttttcg gcgataaaca gtaagttaaa gggaacccga atttagtgct atataattta
     9901 attccactag agtttgtatc ctttgataga tacgcgtatt tcgacctcaa ctgcaaggcc
     9961 gtcgtgtact agacttgact aatccagact ggtcttttag ttatgacttc tgtccacatc
    10021 tccatacatt caacgcactg tgcggctgtg ctgtgcgact ccgtcgagtc gaccaacata
    10081 gttgaaacaa attgaatatt taattgatcg ttataggaat ggtgttagat gagtcatcct
    10141 ttacagtaag cacatacagt attataattg aagatcgtcg gcagataggt gtgtagggta
    10201 gagtatcagc aataagttgg gacgtttgac tttttgtagg tagacaaaaa ctaaactttt
    10261 tttcgcttct ctatgtgtgc ccctgggtag cgttccgttc cgattggggt gcgaacgaat
    10321 gaaatcgccc atcgagttga tacgtccatc catcgctaga accgcgttcg ctgtagaaga
    10381 ctatataaga gcagaggcaa gagtagtgaa atgataggag ccaagttctg gggtcttaga
    10441 gctatgctgg aaacagcata gcaagttaag ataaggctag tccgttatca acttgaaaaa
    10501 gtggcaccga gtcggtgctt tttttggtgg ttgttcatcg attcctaata ttgcagtgaa
    10561 gcagtttgtt gtgcatggcg aagataaggt tagggcgagg aatgccttgg aaataagcgg
    10621 cactgggccg tatgaataaa aagcgttgaa ctttactaca tgtagcatct actcaaaaac
    10681 aaaatccaca aagtttacta cacttttggt atgaataaaa accttttcga acatgtagta
    10741 cctactactt taagaaaatt ctattcaaag tacagagtaa tgctgcatgt taagaaaatt
    10801 ccattcagag tgactcttga aatcaataaa atcatgcata tatgccaaat ttgtcactta
    10861 ttcacgtgac aaattatgtg aaaatatgaa tattactaca ttcccgctat gcatcgcgta
    10921 atcgatcatg aaatttatgt tggttatgta actttacagt attcccttta tgtgcatgtg
    10981 aattttataa tgatttgaca acaaggattt tccttagaat tttcgaattt tagctagaaa
    11041 tctgtatcct atgaatgaat cttgagataa cagcaattgt aacattataa agttcctaac
    11101 attttcaaga ttccagtatt gagtcgcgat cgcttaatta accgcggata tgtcttgccg
    11161 aagcacatta acccaagcca tttatatatc aagtacaagt tgatttgatt ctagtgatgg
    11221 actgacacga atctatggca ctgttgcaga gcttacaaac aggtacccgg agtcgcttca
    11281 attattcaat catccattca aaaacttcca tctcttccaa ttagccaata ttcaaattgg
    11341 aatccagcca gccgacggag gcagatggtc tacctggcaa ctatagagtt tccatgtttc
    11401 cagactttcc ctcccggtaa acggagacaa aacgacagac gtaagtaggt acatatgcat
    11461 accgcacgga caaatcaaat ttgtctggca gctccaatta gagtcgttaa aaatttaacg
    11521 atgcgctaaa taacttcaag ctatttgtct cgctggattg gcttcgagtg gtaagatcct
    11581 atcaaatgcc gaaaacaaaa aactttcttc ttaattgttc gttcttcaac acctctccat
    11641 ggtgataacg gatacggttt cattgtcagc atccatcctc cgaaaaatac attacgcctt
    11701 gaaatatgca atcgcaaaca cggatctgtt tggaacattt attttactat gaagagatgc
    11761 gataggtaat atttatttga gcgtttaaga tactcattgt tctctcaaag aatgtcattg
    11821 aaagccaacg aggtcaaatc aaatattata ataaaaaggt caaagaggac taacttaaag
    11881 ctctctttat ggataggaaa aaatattttc gcccatcgct agaactttta ccgtttccat
    11941 tgagtatata actaagatga atgaggctaa ttgatcacta tggattcggt ccgaggtctt
    12001 agagctatgc tggaaacagc atagcaagtt aagataaggc tagtccgtta tcaacttgaa
    12061 aaagtggcac cgagtcggtg ctttttttaa gatgcttcgg caattaataa taaggaatgg
    12121 taaagcattc ttcaaatgtt actatgttct caaattttca agaattaggt attcaagaaa
    12181 ttctttcagg catattccat aatctgcaca agcagctact ttagaaatta ttgccgggct
    12241 taaattatat gtacaacatt tcctgagtat tttctgacaa aaattctcac aagggaaggt
    12301 cacgatggag ttacacgggg tttttaaccg gactattttt gttagattct acatgtcgaa
    12361 atatgaaaaa ccccaaagcc tttcgggtga tggaccagtg gtgtagccag gaggggctct
    12421 gaaaggggta attttgtacg tatattatat tattaagcta attggaaatt tgacaaaaat
    12481 atttttttag tatttattgt gatggtagag aacaaaattg acattaatgt acgtttgaaa
    12541 tgtatttgcc atgcctttgg cgtactaaca tggagtatgg acaccaaaac cgatttcggc
    12601 cttttgggcc cggcgcgccg ttcccacaat ggttaattcg agctcgcccg gggatctaat
    12661 tcaattagag actaattcaa ttagagctaa ttcaattagg atccaagctt atcgatttcg
    12721 aaccctcgac cgccggagta taaatagagg cgcttcgtct acggagcgac aattcaattc
    12781 aaacaagcaa agtgaacacg tcgctaagcg aaagctaagc aaataaacaa gcgcagctga
    12841 acaagctaaa caatcggggt accgctagag tcgacggtac cgcgggcccg ggatccaccg
    12901 gtcgccacc
//
