LOCUS       sequence-327858-       13582 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses four gRNA targeting Mhc gene with 3xp3-tdTomato marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13582)
  AUTHORS   Li M, Yang T, Bui M, Gamez S, Wise T, Kandul NP, Liu J, Alcantara L,
            Lee H, Edula JR, Raban R, Zhan Y, Wang Y, DeBeaubien N, Chen J, 
            Sanchez C HM, Bennett JB, Antoshechkin I, Montell C, Marshall JM, 
            Akbari OS
  TITLE     Suppressing mosquito populations with precision guided sterile 
            males.
  JOURNAL   Nat Commun. 2021 Sep 10;12(1):5374. doi: 10.1038/s41467-021-25421-w.
  PUBMED    34508072
REFERENCE   2  (bases 1 to 13582)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2021-09-10"; volume: "12(1):5374. doi"; pages: " 
            10.1038/s41467-021-25421-w"
COMMENT     Sequence Label: OA-1067K
FEATURES             Location/Qualifiers
     source          1..13582
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      1..10
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(140..158)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     408..427
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     602..625
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     polyA_signal    1559..1680
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1596..1615)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1650..1669
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     repeat_region   5519..5553
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(5734..5750)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5734..5750)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5747..5769)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5758..5774
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5782..5788,5789..5806,5807..5812))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5827..5848
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5965..5982)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6136..6724)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6216..6235)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6895..7686,7687..7755))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7518..7537
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7756..7860)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     7928..7946
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(7984..8006)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     8106..8125
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8319..8341
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     8333..8350
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     8334..8350
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     repeat_region   9054..9116
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
ORIGIN
        1 gccaccatgg tgagcaaggg cgaggaggtc atcaaagagt tcatgcgctt caaggtgcgc
       61 atggagggct ccatgaacgg ccacgagttc gagatcgagg gcgagggcga gggccgcccc
      121 tacgagggca cccagaccgc caagctgaag gtgaccaagg gcggccccct gcccttcgcc
      181 tgggacatcc tgtcccccca gttcatgtac ggctccaagg cgtacgtgaa gcaccccgcc
      241 gacatccccg attacaagaa gctgtccttc cccgagggct tcaagtggga gcgcgtgatg
      301 aacttcgagg acggcggtct ggtgaccgtg acccaggact cctccctgca ggacggcacg
      361 ctgatctaca aggtgaagat gcgcggcacc aacttccccc ccgacggccc cgtaatgcag
      421 aagaagacca tgggctggga ggcctccacc gagcgcctgt acccccgcga cggcgtgctg
      481 aagggcgaga tccaccaggc cctgaagctg aaggacggcg gccactacct ggtggagttc
      541 aagaccatct acatggccaa gaagcccgtg caactgcccg gctactacta cgtggacacc
      601 aagctggaca tcacctccca caacgaggac tacaccatcg tggaacagta cgagcgctcc
      661 gagggccgcc accacctgtt cctggggcat ggcaccggca gcaccggcag cggcagctcc
      721 ggcaccgcct cctccgagga caacaacatg gccgttatca aggaatttat gcgcttcaaa
      781 gttaggatgg agggatccat gaacggacat gagttcgaga tcgagggaga gggcgaggga
      841 cgcccgtatg aaggcacaca aacagccaaa ctcaaggtca ccaagggcgg accactgccc
      901 ttcgcctggg atatcctgag tccccagttt atgtacggca gcaaggccta cgttaagcac
      961 cccgctgaca taccggacta caaaaagctg tcctttccgg aaggcttcaa gtgggagcgc
     1021 gtgatgaatt tcgaagacgg aggactggtc actgtgaccc aagatagcag tttgcaggac
     1081 ggtacactga tctataaggt taaaatgcgc ggcactaact ttccgccaga tggcccagtg
     1141 atgcagaaga agaccatggg ttgggaggca tccaccgaac gtctgtaccc tcgagacgga
     1201 gtgctcaagg gcgagatcca tcaggccctc aaactgaaag atggtggtca ctacctggtc
     1261 gaatttaaga ccatttacat ggccaagaag ccggttcagc tgcccggata ttattatgtg
     1321 gatacgaaac tggatataac ttcgcataac gaagactaca ccattgtcga gcagtatgag
     1381 cgcagcgaag gccgacatca cctgttcctc tacggcatgg acgagctgta caagtaggcg
     1441 gccgcgactc tagatcataa tcagccatac cacatttgta gaggttttac ttgctttaaa
     1501 aaacctccca cacctccccc tgaacctgaa acataaaatg aatgcaattg ttgttgttaa
     1561 cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa
     1621 taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta
     1681 aagcttgcta gctcatcaca aacgaaacta ttataatcgt cttcagggga acggtagcat
     1741 catatctgtc accagtcagt cggattcctt tctttcggca taaattatgc tcgggtgacg
     1801 agacgagttg aaaatcccgt aaattaaaac atataccaca ctcgccgtcc tagacgacga
     1861 cgacgacgac gattcccaag gaattgaacc cttcatttgc atttcactac cgctctgaga
     1921 gatgcaaagc gcgccagaca gtcgatcgaa tctgcagacc agagagtcac atcaagaatg
     1981 ttgacattcc accagaagac tctaattatt cttcgctcga ctgggaaaac tgttaacaac
     2041 tgctcgagtg tggtcggagc ggctcacagt gcgttttccc gtagacaaaa atcaaatttc
     2101 atggtatttt tcggcgataa acagtaagtt aaagggaacc cgaatttagt gctatataat
     2161 ttaattccac tagagtttgt atcctttgat agatacgcgt atttcgacct caactgcaag
     2221 gccgtcgtgt actagacttg actaatccag actggtcttt tagttatgac ttctgtccac
     2281 atctccatac attcaacgca ctgtgcggct gtgctgtgcg actccgtcga gtcgaccaac
     2341 atagttgaaa caaattgaat atttaattga tcgttatagg aatggtgtta gatgagtcat
     2401 cctttacagt aagcacatac agtattataa ttgaagatcg tcggcagata ggtgtgtagg
     2461 gtagagtatc agcaataagt tgggacgttt gactttttgt aggtagacaa aaactaaact
     2521 ttttttcgct tctctatgtg tgcccctggg tagcgttccg ttccgattgg ggtgcgaacg
     2581 aatgaaatcg cccatcgagt tgatacgtcc atccatcgct agaaccgcgt tcgctgtaga
     2641 agactatata agagcagagg caagagtagt gaaatggctt catctggaac ccagcgtctt
     2701 agagctatgc tggaaacagc atagcaagtt aagataaggc tagtccgtta tcaacttgaa
     2761 aaagtggcac cgagtcggtg ctttttttgg tggttgttca tcgattccta atattgcagt
     2821 gaagcagttt gttgtgcatg gcgaagataa ggttagggcg aggaatgcct tggaaataag
     2881 cggcactggg ccgtatgaat aaaaagcgtt gaactttact acatgtagca tctactcaaa
     2941 aacaaaatcc acaaagttta ctacactttt ggtatgaata aaaacctttt cgaacatgta
     3001 gtacctacta ctttaagaaa attctattca aagtacagag taatgctgca tgttaagaaa
     3061 attccattca gagtgactct tgaaatcaat aaaatcatgc atatatgcca aatttgtcac
     3121 ttattcacgt gacaaattat gtgaaaatat gaatattact acattcccgc tatgcatcgc
     3181 gtaatcgatc atgaaattta tgttggttat gtaactttac agtattccct ttatgtgcat
     3241 gtgaatttta taatgatttg acaacaagga ttttccttag aattttcgaa ttttagctag
     3301 aaatctgtat cctatgaatg aatcttgaga taacagcaat tgtaacatta taaagttcct
     3361 aacattttca agattccagt attgagtcgc gatcgcttaa ttaaccgcgg atatgtcttg
     3421 ccgaagcaca ttaacccaag ccatttatat atcaagtaca agttgatttg attctagtga
     3481 tggactgaca cgaatctatg gcactgttgc agagcttaca aacaggtacc cggagtcgct
     3541 tcaattattc aatcatccat tcaaaaactt ccatctcttc caattagcca atattcaaat
     3601 tggaatccag ccagccgacg gaggcagatg gtctacctgg caactataga gtttccatgt
     3661 ttccagactt tccctcccgg taaacggaga caaaacgaca gacgtaagta ggtacatatg
     3721 cataccgcac ggacaaatca aatttgtctg gcagctccaa ttagagtcgt taaaaattta
     3781 acgatgcgct aaataacttc aagctatttg tctcgctgga ttggcttcga gtggtaagat
     3841 cctatcaaat gccgaaaaca aaaaactttc ttcttaattg ttcgttcttc aacacctctc
     3901 catggtgata acggatacgg tttcattgtc agcatccatc ctccgaaaaa tacattacgc
     3961 cttgaaatat gcaatcgcaa acacggatct gtttggaaca tttattttac tatgaagaga
     4021 tgcgataggt aatatttatt tgagcgttta agatactcat tgttctctca aagaatgtca
     4081 ttgaaagcca acgaggtcaa atcaaatatt ataataaaaa ggtcaaagag gactaactta
     4141 aagctctctt tatggatagg aaaaaatatt ttcgcccatc gctagaactt ttaccgtttc
     4201 cattgagtat ataactaaga tgaatgaggc taattgatgt aaccgttgcc ctacccgggt
     4261 cttagagcta tgctggaaac agcatagcaa gttaagataa ggctagtccg ttatcaactt
     4321 gaaaaagtgg caccgagtcg gtgctttttt taagatgctt cggcaattaa taataaggaa
     4381 tggtaaagca ttcttcaaat gttactatgt tctcaaattt tcaagaatta ggtattcaag
     4441 aaattctttc aggcatattc cataatctgc acaagcagct actttagaaa ttattgccgg
     4501 gcttaaatta tatgtacaac atttcctgag tattttctga caaaaattct cacaagggaa
     4561 ggtcacgatg gagttacacg gggtttttaa ccggactatt tttgttagat tctacatgtc
     4621 gaaatatgaa aaaccccaaa gcctttcggg tgatggacca gtggtgtagc caggaggggc
     4681 tctgaaaggg gtaattttgt acgtatatta tattattaag ctaattggaa atttgacaaa
     4741 aatatttttt tagtatttat tgtgatggta gagaacaaaa ttgacattaa tgtacgtttg
     4801 aaatgtattt gccatgcctt tggcgtacta acatggagta tggacaccaa aaccgatttc
     4861 gtttaaacga tctcggatct gacaatgttc agtgcagaga ctcggctacg cctcgtggac
     4921 tttgaagttg accaacaatg tttattctta cctctaatag tcctctgtgg caaggtcaag
     4981 attctgttag aagccaatga agaacctggt tgttcaataa cattttgttc gtctaatatt
     5041 tcactaccgc ttgacgttgg ctgcacttca tgtacctcat ctataaacgc ttcttctgta
     5101 tcgctctgga cgtcatcttc acttacgtga tctgatattt cactgtcaga atcctcacca
     5161 acaagctcgt catcgctttg cagaagagca gagaggatat gctcatcgtc taaagaacta
     5221 cccattttat tatatattag tcacgatatc tataacaaga aaatatatat ataataagtt
     5281 atcacgtaag tagaacatga aataacaata taattatcgt atgagttaaa tcttaaaagt
     5341 cacgtaaaag ataatcatgc gtcattttga ctcacgcggt cgttatagtt caaaatcagt
     5401 gacacttacc gcattgacaa gcacgcctca cgggagctcc aagcggcgac tgagatgtcc
     5461 taaatgcaca gcgacggatt cgcgctattt agaaagagag agcaatattt caagaatgca
     5521 tgcgtcaatt ttacgcagac tatctttcta gggttaaaaa agatttgcgc tttactcgac
     5581 ctaaacttta aacacgtcat agaatcttcg tttgacaaaa accacattgt ggccaagctg
     5641 tgtgacgcga cgcgcgctaa agaatggcaa accaagtcgc gcgagcgtcg actctagagg
     5701 atccccgggt accgagctcg aattcgtaat catgtcatag ctgtttcctg tgtgaaattg
     5761 ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg
     5821 tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc
     5881 gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt
     5941 gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
     6001 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
     6061 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
     6121 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
     6181 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
     6241 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
     6301 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
     6361 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
     6421 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
     6481 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
     6541 cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct
     6601 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
     6661 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
     6721 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
     6781 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta
     6841 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca
     6901 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc
     6961 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc
     7021 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc
     7081 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat
     7141 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt
     7201 tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc
     7261 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag
     7321 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt
     7381 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac
     7441 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg
     7501 cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat
     7561 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc
     7621 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc
     7681 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa
     7741 atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg
     7801 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg
     7861 cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc attattatca tgacattaac
     7921 ctataaaaat aggcgtatca cgaggccctt tcgtctcgcg cgtttcggtg atgacggtga
     7981 aaacctctga cacatgcagc tcccggagac ggtcacagct tgtctgtaag cggatgccgg
     8041 gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg gctggcttaa
     8101 ctatgcggca tcagagcaga ttgtactgag agtgcaccat atgcggtgtg aaataccgca
     8161 cagatgcgta aggagaaaat accgcatcag gcgccattcg ccattcaggc tgcgcaactg
     8221 ttgggaaggg cgatcggtgc gggcctcttc gctattacgc cagctggcga aagggggatg
     8281 tgctgcaagg cgattaagtt gggtaacgcc agggttttcc cagtcacgac gttgtaaaac
     8341 gacggccagt gccaagcttt gtttaaaaat ataacaaaat tgtgatccca caaaatgaag
     8401 tggggcaaaa tcaaataatt aatagtgtcc gtaaacttgt tggtcttcaa ctttttgagg
     8461 aacacgttgg acggcaaatc cgtgactata acacaagttg atttaataat tttagccaac
     8521 acgtcgggct gcgtgttttt tgccgacgcg tctgtgtaca cgttgattaa ctggtcgatt
     8581 aaactgttga aataatttaa tttttggttc ttctttaaat ctgtgatgaa attttttaaa
     8641 ataactttaa attcttcatt ggtaaaaaat gccacgtttt gcaacttgtg agggtctaat
     8701 atgaggtcaa actcagtagg agttttatcc aaaaaagaaa acatgattac gtctgtacac
     8761 gaacgcgtat taacgcagag tgcaaagtat aagagggtta aaaaatatat tttacgcacc
     8821 atatacgcat cgggttgata tcgttaatat ggatcaattt gaacagttga ttaacgtgtc
     8881 tctgctcaag tctttgatca aaacgcaaat cgacgaaaat gtgtcggaca atatcaagtc
     8941 gatgagcgaa aaactaaaaa ggctagaata cgacaatctc acagacagcg ttgagatata
     9001 cggtattcac gacagcaggc tgaataataa aaaaattaga aactattatt taaccctaga
     9061 aagataatca tattgtgacg tacgttaaag ataatcatgc gtaaaattga cgcatgtgtt
     9121 ttatcggtct gtatatcgag gtttatttat taatttgaat agatattaag ttttattata
     9181 tttacactta catactaata ataaattcaa caaacaattt atttatgttt atttatttat
     9241 taaaaaaaac aaaaactcaa aatttcttct ataaagtaac aaaactttta aacattctct
     9301 cttttacaaa aataaactta ttttgtactt taaaaacagt catgttgtat tataaaataa
     9361 gtaattagct taacttatac ataatagaaa caaattatac ttattagtca gtcagaaaca
     9421 actttggcac atatcaatat tatgctctcg acaaataact tttttgcatt ttttgcacga
     9481 tgcatttgcc tttcgcctta ttttagaggg gcagtaagta cagtaagtac gttttttcat
     9541 tactggctct tcagtactgt catctgatgt accaggcact tcatttggca aaatattaga
     9601 gatattatcg cgcaaatatc tcttcaaagt aggagcttct aaacgcttac gcataaacga
     9661 tgacgtcagg ctcatgtaaa ggtttctcat aaattttttg cgactttgaa ccttttctcc
     9721 cttgctactg acattatggc tgtatataat aaaagaattt atgcaggcaa tgtttatcat
     9781 tccgtacaat aatgccatag gccacctatt cgtcttccta ctgcaggtca tcacagaaca
     9841 catttggtct agcgtgtcca ctccgccttt agtttgatta taatacataa ccatttgcgg
     9901 tttaccggta ctttcgttga tagaagcatc ctcatcacaa gatgataata agtataccat
     9961 cttagctggc ttcggtttat atgagacgag agtaaggggt ccgtcaaaac aaaacatcga
    10021 tgttcccact ggcctggagc gactgttttt cagtacttcc ggtatctcgc gtttgtttga
    10081 tcgcacggtt cccacaatgg ttaattcgag ctcgcccggg ctcctaggag atcttcatca
    10141 caaacgaaac tattataatc gtcttcaggg gaacggtagc atcatatctg tcaccagtca
    10201 gtcggattcc tttctttcgg cataaattat gctcgggtga cgagacgagt tgaaaatccc
    10261 gtaaattaaa acatatacca cactcgccgt cctagacgac gacgacgacg acgattccca
    10321 aggaattgaa cccttcattt gcatttcact accgctctga gagatgcaaa gcgcgccaga
    10381 cagtcgatcg aatctgcaga ccagagagtc acatcaagaa tgttgacatt ccaccagaag
    10441 actctaatta ttcttcgctc gactgggaaa actgttaaca actgctcgag tgtggtcgga
    10501 gcggctcaca gtgcgttttc ccgtagacaa aaatcaaatt tcatggtatt tttcggcgat
    10561 aaacagtaag ttaaagggaa cccgaattta gtgctatata atttaattcc actagagttt
    10621 gtatcctttg atagatacgc gtatttcgac ctcaactgca aggccgtcgt gtactagact
    10681 tgactaatcc agactggtct tttagttatg acttctgtcc acatctccat acattcaacg
    10741 cactgtgcgg ctgtgctgtg cgactccgtc gagtcgacca acatagttga aacaaattga
    10801 atatttaatt gatcgttata ggaatggtgt tagatgagtc atcctttaca gtaagcacat
    10861 acagtattat aattgaagat cgtcggcaga taggtgtgta gggtagagta tcagcaataa
    10921 gttgggacgt ttgacttttt gtaggtagac aaaaactaaa ctttttttcg cttctctatg
    10981 tgtgcccctg ggtagcgttc cgttccgatt ggggtgcgaa cgaatgaaat cgcccatcga
    11041 gttgatacgt ccatccatcg ctagaaccgc gttcgctgta gaagactata taagagcaga
    11101 ggcaagagta gtgaaatgaa atgaagagcc attcggagtc ttagagctat gctggaaaca
    11161 gcatagcaag ttaagataag gctagtccgt tatcaacttg aaaaagtggc accgagtcgg
    11221 tgcttttttt ggtggttgtt catcgattcc taatattgca gtgaagcagt ttgttgtgca
    11281 tggcgaagat aaggttaggg cgaggaatgc cttggaaata agcggcactg ggccgtatga
    11341 ataaaaagcg ttgaacttta ctacatgtag catctactca aaaacaaaat ccacaaagtt
    11401 tactacactt ttggtatgaa taaaaacctt ttcgaacatg tagtacctac tactttaaga
    11461 aaattctatt caaagtacag agtaatgctg catgttaaga aaattccatt cagagtgact
    11521 cttgaaatca ataaaatcat gcatatatgc caaatttgtc acttattcac gtgacaaatt
    11581 atgtgaaaat atgaatatta ctacattccc gctatgcatc gcgtaatcga tcatgaaatt
    11641 tatgttggtt atgtaacttt acagtattcc ctttatgtgc atgtgaattt tataatgatt
    11701 tgacaacaag gattttcctt agaattttcg aattttagct agaaatctgt atcctatgaa
    11761 tgaatcttga gataacagca attgtaacat tataaagttc ctaacatttt caagattcca
    11821 gtattgagtc gcgatcgctt aattaaccgc ggatatgtct tgccgaagca cattaaccca
    11881 agccatttat atatcaagta caagttgatt tgattctagt gatggactga cacgaatcta
    11941 tggcactgtt gcagagctta caaacaggta cccggagtcg cttcaattat tcaatcatcc
    12001 attcaaaaac ttccatctct tccaattagc caatattcaa attggaatcc agccagccga
    12061 cggaggcaga tggtctacct ggcaactata gagtttccat gtttccagac tttccctccc
    12121 ggtaaacgga gacaaaacga cagacgtaag taggtacata tgcataccgc acggacaaat
    12181 caaatttgtc tggcagctcc aattagagtc gttaaaaatt taacgatgcg ctaaataact
    12241 tcaagctatt tgtctcgctg gattggcttc gagtggtaag atcctatcaa atgccgaaaa
    12301 caaaaaactt tcttcttaat tgttcgttct tcaacacctc tccatggtga taacggatac
    12361 ggtttcattg tcagcatcca tcctccgaaa aatacattac gccttgaaat atgcaatcgc
    12421 aaacacggat ctgtttggaa catttatttt actatgaaga gatgcgatag gtaatattta
    12481 tttgagcgtt taagatactc attgttctct caaagaatgt cattgaaagc caacgaggtc
    12541 aaatcaaata ttataataaa aaggtcaaag aggactaact taaagctctc tttatggata
    12601 ggaaaaaata ttttcgccca tcgctagaac ttttaccgtt tccattgagt atataactaa
    12661 gatgaatgag gctaattgat ggtgaaatca aagcaaccaa gtcttagagc tatgctggaa
    12721 acagcatagc aagttaagat aaggctagtc cgttatcaac ttgaaaaagt ggcaccgagt
    12781 cggtgctttt tttaagatgc ttcggcaatt aataataagg aatggtaaag cattcttcaa
    12841 atgttactat gttctcaaat tttcaagaat taggtattca agaaattctt tcaggcatat
    12901 tccataatct gcacaagcag ctactttaga aattattgcc gggcttaaat tatatgtaca
    12961 acatttcctg agtattttct gacaaaaatt ctcacaaggg aaggtcacga tggagttaca
    13021 cggggttttt aaccggacta tttttgttag attctacatg tcgaaatatg aaaaacccca
    13081 aagcctttcg ggtgatggac cagtggtgta gccaggaggg gctctgaaag gggtaatttt
    13141 gtacgtatat tatattatta agctaattgg aaatttgaca aaaatatttt tttagtattt
    13201 attgtgatgg tagagaacaa aattgacatt aatgtacgtt tgaaatgtat ttgccatgcc
    13261 tttggcgtac taacatggag tatggacacc aaaaccgatt tcggcctttt gggcccggcg
    13321 cgccaattcg agctcgcccg gggatctaat tcaattagag actaattcaa ttagagctaa
    13381 ttcaattagg atccaagctt atcgatttcg aaccctcgac cgccggagta taaatagagg
    13441 cgcttcgtct acggagcgac aattcaattc aaacaagcaa agtgaacacg tcgctaagcg
    13501 aaagctaagc aaataaacaa gcgcagctga acaagctaaa caatcggggt accgctagag
    13561 tcgacggtac gatccaccgg tc
//
