LOCUS       sequence-327859-       10957 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of FLAG-tagged Tapasin.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 10957)
  AUTHORS   McShan AC, Devlin CA, Morozov GI, Overall SA, Moschidi D, Akella N, 
            Procko E, Sgourakis NG
  TITLE     TAPBPR promotes antigen loading on MHC-I molecules using a peptide 
            trap.
  JOURNAL   Nat Commun. 2021 May 26;12(1):3174. doi: 10.1038/s41467-021-23225-6.
  PUBMED    34039964
REFERENCE   2  (bases 1 to 10957)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2021-05-26"; volume: "12(1):3174. doi"; pages: " 
            10.1038/s41467-021-23225-6"
COMMENT     Sequence Label: pCEP4-FLAG-Tapasin
FEATURES             Location/Qualifiers
     source          1..10957
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(798..816)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        884..988
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(989..1057,1058..1849)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1207..1226)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2020..2608
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2509..2528
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        3039..3184
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     CDS             3227..4264
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPDRE
                     MGEAN"
     primer_bind     complement(4262..4281)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    4306..4354
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     enhancer        5008..5387
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        5388..5591
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     5542..5562
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     5588..5612
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      5640..5649
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5724..5747
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     polyA_signal    7066..7200
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7090..7109)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     7144..7163
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(7343..7365)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      7925..9714
                     /label=oriP
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
ORIGIN
        1 gcctccacct cctgctcctg ccnnncctcc tgctcctgcc cctcctcctg ctcctgcccc
       61 tcctgctcct gcccctcccg ctcctgctcc tgctcctgtt ccaccgtggg tccctttgca
      121 gccaatgcaa cttggacgtt tttggggtct ccggacacca tctctatgtc ttggccctga
      181 tcctgagccg cccggggctc ctggtcttcc gcctcctcgt cctcgtcctc ttccccgtcc
      241 tcgtccatgg ttatcacccc ctcttctttg aggtccactg ccgccggagc cttctggtcc
      301 agatgtgtct cccttctctc ctaggccatt tccaggtcct gtacctggcc cctcgtcaga
      361 catgattcac actaaaagag atcaatagac atctttatta gacgacgctc agtgaataca
      421 gggagtgcag actcctgccc cctccaacag cccccccacc ctcatcccct tcatggtcgc
      481 tgtcagacag atccaggtct gaaaattccc catcctccga accatcctcg tcctcatcac
      541 caattactcg cagcccggaa aactcccgct gaacatcctc aagatttgcg tcctgagcct
      601 caagccaggc ctcaaattcc tcgtccccct ttttgctgga cggtagggat ggggattctc
      661 gggacccctc ctcttcctct tcaaggtcac cagacagaga tgctactggg gcaacggaag
      721 aaaagctggg tgcggcctgt gaggatcagc ttatcgatga taagctgtca aacatgagaa
      781 ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat
      841 aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg
      901 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat
      961 gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat
     1021 tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt
     1081 aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag
     1141 cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa
     1201 agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg
     1261 ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct
     1321 tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac
     1381 tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca
     1441 caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat
     1501 accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact
     1561 attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc
     1621 ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga
     1681 taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg
     1741 taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg
     1801 aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca
     1861 agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta
     1921 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca
     1981 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
     2041 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
     2101 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
     2161 tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
     2221 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
     2281 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
     2341 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
     2401 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
     2461 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
     2521 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
     2581 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
     2641 ggccttttgc tggccttgaa gctgtccctg atggtcgtca tctacctgcc tggacagcat
     2701 ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa tggggaaggc
     2761 catccagcct cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg ccccgagatg
     2821 cgccgcgtgc ggctgctgga gatggcggac gcgatggata tgttctgcca agggttggtt
     2881 tgcgcattca cagttctccg caagaattga ttggctccaa ttcttggagt ggtgaatccg
     2941 ttagcgaggt gccgccctgc ttcatccccg tggcccgttg ctcgcgtttg ctggcggtgt
     3001 ccccggaaga aatatatttg catgtcttta gttctatgat gacacaaacc ccgcccagcg
     3061 tcttgtcatt ggcgaattcg aacacgcaga tgcagtcggg gcggcgcggt ccgaggtcca
     3121 cttcgcatat taaggtgacg cgtgtggcct cgaacaccga gcgaccctgc agcgacccgc
     3181 ttaacagcgt caacagcgtg ccgcagatcc cggggggcaa tgagatatga aaaagcctga
     3241 actcaccgcg acgtctgtcg agaagtttct gatcgaaaag ttcgacagcg tctccgacct
     3301 gatgcagctc tcggagggcg aagaatctcg tgctttcagc ttcgatgtag gagggcgtgg
     3361 atatgtcctg cgggtaaata gctgcgccga tggtttctac aaagatcgtt atgtttatcg
     3421 gcactttgca tcggccgcgc tcccgattcc ggaagtgctt gacattgggg aattcagcga
     3481 gagcctgacc tattgcatct cccgccgtgc acagggtgtc acgttgcaag acctgcctga
     3541 aaccgaactg cccgctgttc tgcagccggt cgcggaggcc atggatgcga tcgctgcggc
     3601 cgatcttagc cagacgagcg ggttcggccc attcggaccg caaggaatcg gtcaatacac
     3661 tacatggcgt gatttcatat gcgcgattgc tgatccccat gtgtatcact ggcaaactgt
     3721 gatggacgac accgtcagtg cgtccgtcgc gcaggctctc gatgagctga tgctttgggc
     3781 cgaggactgc cccgaagtcc ggcacctcgt gcacgcggat ttcggctcca acaatgtcct
     3841 gacggacaat ggccgcataa cagcggtcat tgactggagc gaggcgatgt tcggggattc
     3901 ccaatacgag gtcgccaaca tcttcttctg gaggccgtgg ttggcttgta tggagcagca
     3961 gacgcgctac ttcgagcgga ggcatccgga gcttgcagga tcgccgcggc tccgggcgta
     4021 tatgctccgc attggtcttg accaactcta tcagagcttg gttgacggca atttcgatga
     4081 tgcagcttgg gcgcagggtc gatgcgacgc aatcgtccga tccggagccg ggactgtcgg
     4141 gcgtacacaa atcgcccgca gaagcgcggc cgtctggacc gatggctgtg tagaagtact
     4201 cgccgatagt ggaaaccgac gccccagcac tcgtccggat cgggagatgg gggaggctaa
     4261 ctgaaacacg gaaggagaca ataccggaag gaacccgcgc tatgacggca ataaaaagac
     4321 agaataaaac gcacgggtgt tgggtcgttt gttcataaac gcggggttcg gtcccagggc
     4381 tggcactctg tcgatacccc accgagaccc cattggggcc aatacgcccg cgtttcttcc
     4441 ttttccccac cccacccccc aagttcgggt gaaggcccag ggctcgcagc caacgtcggg
     4501 gcggcaggcc ctgccatagc cactggcccc gtgggttagg gacggggtcc cccatgggga
     4561 atggtttatg gttcgtgggg gttattattt tgggcgttgc gtggggtcag gtccacgact
     4621 ggactgagca gacagaccca tggtttttgg atggcctggg catggaccgc atgtactggc
     4681 gcgacacgaa caccgggcgt ctgtggctgc caaacacccc cgacccccaa aaaccaccgc
     4741 gcggatttct ggcgtgccaa gctagtcgac caattctcat gtttgacagc ttatcatcgc
     4801 agatccgggc aacgttgttg ccattgctgc aggcgcagaa ctggtaggta tggaagatct
     4861 atacattgaa tcaatattgg caattagcca tattagtcat tggttatata gcataaatca
     4921 atattggcta ttggccattg catacgttgt atctatatca taatatgtac atttatattg
     4981 gctcatgtcc aatatgaccg ccatgttgac attgattatt gactagttat taatagtaat
     5041 caattacggg gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg
     5101 taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt
     5161 atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac
     5221 ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg
     5281 acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact
     5341 ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt
     5401 ggcagtacac caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc
     5461 ccattgacgt caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc
     5521 gtaataaccc cgccccgttg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata
     5581 taagcagagc tcgtttagtg aaccgtcaga tctctagaag ctgggtacca gctgctagcg
     5641 ccaccatggc cgtcatggcg ccccgaaccc tcgtcctgct actctcgggg gccctggccc
     5701 tgacccagac ctgggcgggc tccgactaca aggacgatga tgacaaggga ggatccggcc
     5761 ctgccgtaat cgagtgttgg tttgttgaag acgctagtgg taaagggctg gctaaacggc
     5821 caggcgcact tctgttgcgg caaggtccgg gagaaccacc tccacgaccc gacttggacc
     5881 ctgaattgta cctctctgta cacgacccag ctggagcact ccaagctgct ttccgacggt
     5941 acccaagagg tgctccagca ccgcattgcg agatgagtcg gttcgttcct ttgcctgcga
     6001 gtgcaaagtg ggcatcaggt ctgacaccag cacaaaactg tccacgagcg cttgatggag
     6061 cgtggctgat ggtttccata agctctccgg tgctttcact gtcatcactg ctcaggccac
     6121 aacctgaacc ccaacaagaa cccgtactga taacaatggc gacggtagtc cttacggtcc
     6181 ttacgcatac accagcaccc agggtgcgat tgggacaaga cgcactcctc gaccttagct
     6241 ttgcgtacat gccaccaacc tcagaggcgg cttcctctct tgcaccagga ccaccaccgt
     6301 tcggccttga atggagacga cagcacttgg ggaagggcca tttgctgttg gctgctaccc
     6361 cagggctgaa cgggcagatg ccggcagcac aagagggagc cgtagctttt gctgcgtggg
     6421 atgatgacga accatgggga ccatggactg gaaacgggac tttctggctg ccaacagtac
     6481 agccctttca agaaggaacg tatctggcca ccatccattt gccttatctg caaggccagg
     6541 tcacacttga actcgctgtc tataaaccgc ctaaggtgag tctcatgccc gcaactttgg
     6601 ctagggcagc acctggcgaa gctccacctg aattgctctg tttggtatcc catttctacc
     6661 ccagtggtgg ccttgaagtg gaatgggaac ttagaggagg accaggtggt cgatctcaga
     6721 aagctgaagg gcagcgatgg ctctccgctc tcaggcacca ctcagacgga tctgtatcac
     6781 tgtcagggca tttgcagcca ccgccagtta ccaccgagca gcatggagct cggtatgcgt
     6841 gtcgcattca ccaccctagc cttccggcgt caggaagatc agcggaggta acacttgagg
     6901 tcgctgggtt gtccggaccc agcttggagg acagtgttgg tctgttcctg tccgctttcc
     6961 tgttgctggg tctgtttaag gcactcggat gggcagcagt ttatctcagc acctgtaaag
     7021 acagcaagaa aaaggcagaa tagtaactcg aggccggcaa ggccggatcc agacatgata
     7081 agatacattg atgagtttgg acaaaccaca actagaatgc agtgaaaaaa atgctttatt
     7141 tgtgaaattt gtgatgctat tgctttattt gtaaccatta taagctgcaa taaacaagtt
     7201 aacaacaaca attgcattca ttttatgttt caggttcagg gggaggtgtg ggaggttttt
     7261 taaagcaagt aaaacctcta caaatgtggt atggctgatt atgatccggc tgcctcgcgc
     7321 gtttcggtga tgacggtgaa aacctctgac acatgcagct cccggagacg gtcacagctt
     7381 gtctgtaagc ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg
     7441 ggtgtcgggg cgcagccatg aggtcgactc tagaggatcg atccccgccc cggacgaact
     7501 aaacctgact acgacatctc tgccccttct tcgcggggca gtgcatgtaa tcccttcagt
     7561 tggttggtac aacttgccaa ctgggccctg ttccacatgt gacacggggg gggaccaaac
     7621 acaaaggggt tctctgactg tagttgacat ccttataaat ggatgtgcac atttgccaac
     7681 actgagtggc tttcatcctg gagcagactt tgcagtctgt ggactgcaac acaacattgc
     7741 ctttatgtgt aactcttggc tgaagctctt acaccaatgc tgggggacat gtacctccca
     7801 ggggcccagg aagactacgg gaggctacac caacgtcaat cagaggggcc tgtgtagcta
     7861 ccgataagcg gaccctcaag agggcattag caatagtgtt tataaggccc ccttgttaac
     7921 cctaaacggg tagcatatgc ttcccgggta gtagtatata ctatccagac taaccctaat
     7981 tcaatagcat atgttaccca acgggaagca tatgctatcg aattagggtt agtaaaaggg
     8041 tcctaaggaa cagcgatatc tcccacccca tgagctgtca cggttttatt tacatggggt
     8101 caggattcca cgagggtagt gaaccatttt agtcacaagg gcagtggctg aagatcaagg
     8161 agcgggcagt gaactctcct gaatcttcgc ctgcttcttc attctccttc gtttagctaa
     8221 tagaataact gctgagttgt gaacagtaag gtgtatgtga ggtgctcgaa aacaaggttt
     8281 caggtgacgc ccccagaata aaatttggac ggggggttca gtggtggcat tgtgctatga
     8341 caccaatata accctcacaa accccttggg caataaatac tagtgtagga atgaaacatt
     8401 ctgaatatct ttaacaatag aaatccatgg ggtggggaca agccgtaaag actggatgtc
     8461 catctcacac gaatttatgg ctatgggcaa cacataatcc tagtgcaata tgatactggg
     8521 gttattaaga tgtgtcccag gcagggacca agacaggtga accatgttgt tacactctat
     8581 ttgtaacaag gggaaagaga gtggacgccg acagcagcgg actccactgg ttgtctctaa
     8641 cacccccgaa aattaaacgg ggctccacgc caatggggcc cataaacaaa gacaagtggc
     8701 cactcttttt tttgaaattg tggagtgggg gcacgcgtca gcccccacac gccgccctgc
     8761 ggttttggac tgtaaaataa gggtgtaata acttggctga ttgtaacccc gctaaccact
     8821 gcggtcaaac cacttgccca caaaaccact aatggcaccc cggggaatac ctgcataagt
     8881 aggtgggcgg gccaagatag gggcgcgatt gctgcgatct ggaggacaaa ttacacacac
     8941 ttgcgcctga gcgccaagca cagggttgtt ggtcctcata ttcacgaggt cgctgagagc
     9001 acggtgggct aatgttgcca tgggtagcat atactaccca aatatctgga tagcatatgc
     9061 tatcctaatc tatatctggg tagcataggc tatcctaatc tatatctggg tagcatatgc
     9121 tatcctaatc tatatctggg tagtatatgc tatcctaatt tatatctggg tagcataggc
     9181 tatcctaatc tatatctggg tagcatatgc tatcctaatc tatatctggg tagtatatgc
     9241 tatcctaatc tgtatccggg tagcatatgc tatcctaata gagattaggg tagtatatgc
     9301 tatcctaatt tatatctggg tagcatatac tacccaaata tctggatagc atatgctatc
     9361 ctaatctata tctgggtagc atatgctatc ctaatctata tctgggtagc ataggctatc
     9421 ctaatctata tctgggtagc atatgctatc ctaatctata tctgggtagt atatgctatc
     9481 ctaatttata tctgggtagc ataggctatc ctaatctata tctgggtagc atatgctatc
     9541 ctaatctata tctgggtagt atatgctatc ctaatctgta tccgggtagc atatgctatc
     9601 ctcatgcata tacagtcagc atatgatacc cagtagtaga gtgggagtgc tatcctttgc
     9661 atatgccgcc acctcccaag ggggcgtgaa ttttcgctgc ttgtcctttt cctgctggtt
     9721 gctcccattc ttaggtgaat ttaaggaggc caggctaaag ccgtcgcatg tctgattgct
     9781 caccaggtaa atgtcgctaa tgttttccaa cgcgagaagg tgttgagcgc ggagctgagt
     9841 gacgtgacaa catgggtatg cccaattgcc ccatgttggg aggacgaaaa tggtgacaag
     9901 acagatggcc agaaatacac caacagcacg catgatgtct actggggatt tattctttag
     9961 tgcgggggaa tacacggctt ttaatacgat tgagggcgtc tcctaacaag ttacatcact
    10021 cctgcccttc ctcaccctca tctccatcac ctccttcatc tccgtcatct ccgtcatcac
    10081 cctccgcggc agccccttcc accataggtg gaaaccaggg aggcaaatct actccatcgt
    10141 caaagctgca cacagtcacc ctgatattgc aggtaggagc gggctttgtc ataacaaggt
    10201 ccttaatcgc atccttcaaa acctcagcaa atatatgagt ttgtaaaaag accatgaaat
    10261 aacagacaat ggactccctt agcgggccag gttgtgggcc gggtccaggg gccattccaa
    10321 aggggagacg actcaatggt gtaagacgac attgtggaat agcaagggca gttcctcgcc
    10381 ttaggttgta aagggaggtc ttactacctc catatacgaa cacaccggcg acccaagttc
    10441 cttcgtcggt agtcctttct acgtgactcc tagccaggag agctcttaaa ccttctgcaa
    10501 tgttctcaaa tttcgggttg gaacctcctt gaccacgatg ctttccaaac caccctcctt
    10561 ttttgcgcct gcctccatca ccctgacccc ggggtccagt gcttgggcct tctcctgggt
    10621 catctgcggg gccctgctct atcgctcccg ggggcacgtc aggctcacca tctgggccac
    10681 cttcttggtg gtattcaaaa taatcggctt cccctacagg gtggaaaaat ggccttctac
    10741 ctggaggggg cctgcgcggt ggagacccgg atgatgatga ctgactactg ggactcctgg
    10801 gcctcttttc tccacgtcca cgacctctcc ccctggctct ttcacgactt ccccccctgg
    10861 ctctttcacg tcctctaccc cggcggcctc cactacctcc tcgaccccgg cctccactac
    10921 ctcctcgacc ccggcctcca ctgcctcctc gaccccg
//
