LOCUS       sequence-327861-       11819 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Tetracycline inducible CRE expression from AAVS1 locus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11819)
  AUTHORS   Benito-Kwiecinski S, Giandomenico SL, Sutcliffe M, Riis ES, 
            Freire-Pritchett P, Kelava I, Wunderlich S, Martin U, Wray GA, 
            McDole K, Lancaster MA
  TITLE     An early cell shape transition drives evolutionary expansion of the 
            human forebrain.
  JOURNAL   Cell. 2021 Apr 15;184(8):2084-2102.e19. doi: 
            10.1016/j.cell.2021.02.050. Epub 2021 Mar 24.
  PUBMED    33765444
REFERENCE   2  (bases 1 to 11819)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2021.02.050"; journalName: "Cell"; date: 
            "2021-04-15- 15"; volume: "184"; issue: "8"; pages: "2084-2102.e19"
COMMENT     Sequence Label: AAVS1-Neo-TRE-CMV-Cre-rtTA
FEATURES             Location/Qualifiers
     source          1..11819
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    33..836
                     /label=HA-L
                     /note="left homology arm from the adeno-associated virus 
                     integration site (AAVS1) within intron 1 of the human 
                     PPP1R12C gene"
     misc_feature    843..868
                     /label=SA
                     /note="splice acceptor site"
     CDS             892..945
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             961..1764
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ
                     GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
                     LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ
                     DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     promoter        2018..2332
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    2026..2044
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2062..2080
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2097..2115
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2133..2151
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2169..2187
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2204..2222
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2240..2258
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     2305..2329
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      2395..2404
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2401..3435
                     /codon_start=1
                     /product="site-specific recombinase"
                     /label=Cre
                     /note="Cre is a site-specific recombinase from 
                     bacteriophage P1. Recombination occurs at loxP sequences."
                     /translation="MANLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
                     LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
                     PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
                     LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
                     RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
                     RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
                     DGDG"
     primer_bind     complement(3541..3558)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3547..3771
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     enhancer        4126..4429
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4431..4708
                     /label=chicken beta-actin promoter
     intron          4709..5725
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     5649..5671
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     CDS             join(5825..6442,6443..6448,6449..6484,6485..6487,6488..6523,
                     6524..6526,6527..6562,6563..6571)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPADALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     polyA_signal    6581..6715
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6605..6624)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     6659..6678
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     misc_feature    7055..7891
                     /label=HA-R
                     /note="right homology arm from the adeno-associated virus 
                     integration site (AAVS1) within intron 1 of the human 
                     PPP1R12C gene"
     primer_bind     complement(7925..7944)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7926..7944)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7951..7968)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7951..7967)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7960..7982)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     CDS             8105..8407
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="QFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKV
                     SRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     8286..8305
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     CDS             complement(join(9804..10595,10596..10664))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10427..10446
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10788..11376
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11277..11296
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11530..11547
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    11664..11685
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(11700..11705,11706..11723,11724..11730)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11738..11754
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     11743..11765
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     11762..11778
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     11762..11778
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        11799..11817
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ctagtcctgc aggtttaaac gaattcgccc tttgctttct ctgaccagca ttctctcccc
       61 tgggcctgtg ccgctttctg tctgcagctt gtggcctggg tcacctctac ggctggccca
      121 gatccttccc tgccgcctcc ttcaggttcc gtcttcctcc actccctctt ccccttgctc
      181 tctgctgtgt tgctgcccaa ggatgctctt tccggagcac ttccttctcg gcgctgcacc
      241 acgtgatgtc ctctgagcgg atcctccccg tgtctgggtc ctctccgggc atctctcctc
      301 cctcacccaa ccccatgccg tcttcactcg ctgggttccc ttttccttct ccttctgggg
      361 cctgtgccat ctctcgtttc ttaggatggc cttctccgac ggatgtctcc cttgcgtccc
      421 gcctcccctt cttgtaggcc tgcatcatca ccgtttttct ggacaacccc aaagtacccc
      481 gtctccctgg ctttagccac ctctccatcc tcttgctttc tttgcctgga caccccgttc
      541 tcctgtggat tcgggtcacc tctcactcct ttcatttggg cagctcccct acccccctta
      601 cctctctagt ctgtgctagc tcttccagcc ccctgtcatg gcatcttcca ggggtccgag
      661 agctcagcta gtcttcttcc tccaacccgg gcccctatgt ccacttcagg acagcatgtt
      721 tgctgcctcc agggatcctg tgtccccgag ctgggaccac cttatattcc cagggccggt
      781 taatgtggct ctggttctgg gtacttttat ctgtcccctc caccccacag tggggcaagc
      841 ttctgacctc ttctcttcct cccacagggc ctcgagagat ctggcagcgg agagggcaga
      901 ggaagtcttc taacatgcgg tgacgtggag gagaatcccg gccctaggct cgagacgcgt
      961 atgggatcgg ccattgaaca agatggattg cacgcaggtt ctccggccgc ttgggtggag
     1021 aggctattcg gctatgactg ggcacaacag acaatcggct gctctgatgc cgccgtgttc
     1081 cggctgtcag cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc cggtgccctg
     1141 aatgaactgc aggacgaggc agcgcggcta tcgtggctgg ccacgacggg cgttccttgc
     1201 gcagctgtgc tcgacgttgt cactgaagcg ggaagggact ggctgctatt gggcgaagtg
     1261 ccggggcagg atctcctgtc atctcacctt gctcctgccg agaaagtatc catcatggct
     1321 gatgcaatgc ggcggctgca tacgcttgat ccggctacct gcccattcga ccaccaagcg
     1381 aaacatcgca tcgagcgagc acgtactcgg atggaagccg gtcttgtcga tcaggatgat
     1441 ctggacgaag agcatcaggg gctcgcgcca gccgaactgt tcgccaggct caaggcgcgc
     1501 atgcccgacg gcgaggatct cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg
     1561 gtggaaaatg gccgcttttc tggattcatc gactgtggcc ggctgggtgt ggcggaccgc
     1621 tatcaggaca tagcgttggc tacccgtgat attgctgaag agcttggcgg cgaatgggct
     1681 gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat cgccttctat
     1741 cgccttcttg acgagttctt ctgaggggat ccgctgtaag tctgcagaaa ttgatgatct
     1801 attaaacaat aaagatgtcc actaaaatgg aagtttttcc tgtcatactt tgttaagaag
     1861 ggtgagaaca gagtacctac attttgaatg gaaggattgg agctacgggg gtgggggtgg
     1921 ggtgggatta gataaatgcc tgctctttac tgaaggctct ttactattgc tttatgataa
     1981 tgtttcatag ttggatctag taaagcttag tactgtcgag tttactccct atcagtgata
     2041 gagaacgtat gtcgagttta ctccctatca gtgatagaga acgatgtcga gtttactccc
     2101 tatcagtgat agagaacgta tgtcgagttt actccctatc agtgatagag aacgtatgtc
     2161 gagtttactc cctatcagtg atagagaacg tatgtcgagt ttatccctat cagtgataga
     2221 gaacgtatgt cgagtttact ccctatcagt gatagagaac gtatgtcgag gtaggcgtgt
     2281 acggtgggag gcctatataa gcagagctcg tttagtgaac cgtcagatcg cctgtgtcga
     2341 cgcaacgtgc tggttattgt gctgtctcat cattttggca aagaattctg agccgccacc
     2401 atggccaatt tactgaccgt acaccaaaat ttgcctgcat taccggtcga tgcaacgagt
     2461 gatgaggttc gcaagaacct gatggacatg ttcagggatc gccaggcgtt ttctgagcat
     2521 acctggaaaa tgcttctgtc cgtttgccgg tcgtgggcgg catggtgcaa gttgaataac
     2581 cggaaatggt ttcccgcaga acctgaagat gttcgcgatt atcttctata tcttcaggcg
     2641 cgcggtctgg cagtaaaaac tatccagcaa catttgggcc agctaaacat gcttcatcgt
     2701 cggtccgggc tgccacgacc aagtgacagc aatgctgttt cactggttat gcggcggatc
     2761 cgaaaagaaa acgttgatgc cggtgaacgt gcaaaacagg ctctagcgtt cgaacgcact
     2821 gatttcgacc aggttcgttc actcatggaa aatagcgatc gctgccagga tatacgtaat
     2881 ctggcatttc tggggattgc ttataacacc ctgttacgta tagccgaaat tgccaggatc
     2941 agggttaaag atatctcacg tactgacggt gggagaatgt taatccatat tggcagaacg
     3001 aaaacgctgg ttagcaccgc aggtgtagag aaggcactta gcctgggggt aactaaactg
     3061 gtcgagcgat ggatttccgt ctctggtgta gctgatgatc cgaataacta cctgttttgc
     3121 cgggtcagaa aaaatggtgt tgccgcgcca tctgccacca gccagctatc aactcgcgcc
     3181 ctggaaggga tttttgaagc aactcatcga ttgatttacg gcgctaagga tgactctggt
     3241 cagagatacc tggcctggtc tggacacagt gcccgtgtcg gagccgcgcg agatatggcc
     3301 cgcgctggag tttcaatacc ggagatcatg caagctggtg gctggaccaa tgtaaatatt
     3361 gtcatgaact atatccgtaa cctggatagt gaaacagggg caatggtgcg cctgctggaa
     3421 gatggcgatg gatgaggtac cgagctcgga tccactagtc cagtgtggtg gaattctgca
     3481 gatatccagc acagtggcgg ccgctcgagt ctagagggcc cgtttaaacc cgctgatcag
     3541 cctcgactgt gccttctagt tgccagccat ctgttgtttg cccctccccc gtgccttcct
     3601 tgaccctgga aggtgccact cccactgtcc tttcctaata aaatgaggaa attgcatcgc
     3661 attgtctgag taggtgtcat tctattctgg ggggtggggt ggggcaggac agcaaggggg
     3721 aggattggga agacaatagc aggcatgctg gggatgcggt gggctctatg gcttctgagg
     3781 cggaaagaaa tcataattta aacaagcaaa accaaattaa gggccagctc attcctccca
     3841 ctcatgatct atagatctat agatctctcg tgggatcatt gtttttctct tgattcccac
     3901 tttgtggttc taagtactgt ggtttccaaa tgtgtcagtt tcatagcctg aagaacgaga
     3961 tcagcagcct ctgttccaca tacacttcat tctcagtatt gttttgccaa gttctaattc
     4021 catcagaagc tgggagctcc accgcggtgg cggccgctct agaactagtt attaatagta
     4081 atcaattacg gggtcattag ttcatagccc atatatggag ttccgcgtta cataacttac
     4141 ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc ccattgacgt caataatgac
     4201 gtatgttccc atagtaacgc caatagggac tttccattga cgtcaatggg tggagtattt
     4261 acggtaaact gcccacttgg cagtacatca agtgtatcat atgccaagta cgccccctat
     4321 tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc cagtacatga ccttatggga
     4381 ctttcctact tggcagtaca tctacgtatt agtcatcgct attaccatgg tcgaggtgag
     4441 ccccacgttc tgcttcactc tccccatctc ccccccctcc ccacccccaa ttttgtattt
     4501 atttattttt taattatttt gtgcagcgat gggggcgggg gggggggggg ggcgcgcgcc
     4561 aggcggggcg gggcggggcg aggggcgggg cggggcgagg cggagaggtg cggcggcagc
     4621 caatcagagc ggcgcgctcc gaaagtttcc ttttatggcg aggcggcggc ggcggcggcc
     4681 ctataaaaag cgaagcgcgc ggcgggcggg agtcgctgcg cgctgccttc gccccgtgcc
     4741 ccgctccgcc gccgcctcgc gccgcccgcc ccggctctga ctgaccgcgt tactcccaca
     4801 ggtgagcggg cgggacggcc cttctcctcc gggctgtaat tagcgcttgg tttaatgacg
     4861 gcttgtttct tttctgtggc tgcgtgaaag ccttgagggg ctccgggagg gccctttgtg
     4921 cggggggagc ggctcggggg gtgcgtgcgt gtgtgtgtgc gtggggagcg ccgcgtgcgg
     4981 ctccgcgctg cccggcggct gtgagcgctg cgggcgcggc gcggggcttt gtgcgctccg
     5041 cagtgtgcgc gaggggagcg cggccggggg cggtgccccg cggtgcgggg ggggctgcga
     5101 ggggaacaaa ggctgcgtgc ggggtgtgtg cgtggggggg tgagcagggg gtgtgggcgc
     5161 gtcggtcggg ctgcaacccc ccctgcaccc ccctccccga gttgctgagc acggcccggc
     5221 ttcgggtgcg gggctccgta cggggcgtgg cgcggggctc gccgtgccgg gcggggggtg
     5281 gcggcaggtg ggggtgccgg gcggggcggg gccgcctcgg gccggggagg gctcggggga
     5341 ggggcgcggc ggcccccgga gcgccggcgg ctgtcgaggc gcggcgagcc gcagccattg
     5401 ccttttatgg taatcgtgcg agagggcgca gggacttcct ttgtcccaaa tctgtgcgga
     5461 gccgaaatct gggaggcgcc gccgcacccc ctctagcggg cgcggggcga agcggtgcgg
     5521 cgccggcagg aaggaaatgg gcggggaggg ccttcgtgcg tcgccgcgcc gccgtcccct
     5581 tctccctctc cagcctcggg gctgtccgcg gggggacggc tgccttcggg ggggacgggg
     5641 cagggcgggg ttcggcttct ggcgtgtgac cggcggctct agagcctctg ctaaccatgt
     5701 tcatgccttc ttctttttcc tacagctcct gggcaacgtg ctggttattg tgctgtctca
     5761 tcattttggc aaagaattcg gcttgatcga agctagtgga tcccccgggc tgcaggaatt
     5821 caccatgtct agactggaca agagcaaagt cataaacggc gctctggaat tactcaatgg
     5881 agtcggtatc gaaggcctga cgacaaggaa actcgctcaa aagctgggag ttgagcagcc
     5941 taccctgtac tggcacgtga agaacaagcg ggccctgctc gatgccctgc caatcgagat
     6001 gctggacagg catcataccc acttctgccc cctggaaggc gagtcatggc aagactttct
     6061 gcggaacaac gccaagtcat tccgctgtgc tctcctctca catcgcgacg gggctaaagt
     6121 gcatctcggc acccgcccaa cagagaaaca gtacgaaacc ctggaaaatc agctcgcgtt
     6181 cctgtgtcag caaggcttct ccctggagaa cgcactgtac gctctgtccg ccgtgggcca
     6241 ctttacactg ggctgcgtat tggaggaaca ggagcatcaa gtagcaaaag aggaaagaga
     6301 gacacctacc accgattcta tgcccccact tctgagacaa gcaattgagc tgttcgaccg
     6361 gcagggagcc gaacctgcct tccttttcgg cctggaacta atcatatgtg gcctggagaa
     6421 acagctaaag tgcgaaagcg gcgggccggc cgacgccctt gacgattttg acttagacat
     6481 gctcccagcc gatgcccttg acgactttga ccttgatatg ctgcctgctg acgctcttga
     6541 cgattttgac cttgacatgc tccccgggta actaagtaag gatccagaca tgataagata
     6601 cattgatgag tttggacaaa ccacaactag aatgcagtga aaaaaatgct ttatttgtga
     6661 aatttgtgat gctattgctt tatttgtaac cattataagc tgcaataaac aagttaacaa
     6721 caacaattgc attcatttta tgtttcaggt tcagggggag gtgtgggagg ttttttaaag
     6781 caagtaaaac ctctacaaat gtggtatggc tgattatgat cctgcaagcc tcgtcgtcct
     6841 ggccggacca cgctatctgt gcaaggtccc cggccccgga cgcgcgctcc atgagcagag
     6901 cgcccgccgc cgaggcgaag actcgggcgg cgccctgccc gtcccaccag gtcaacaggc
     6961 ggtaaccggc ctcttcatcg ggaatgcgcg cgaccttcag catcgccggc atgtccccct
     7021 ggcggacggg aagtatccag ctcgaccaag ctttactagg gacaggattg gtgacagaaa
     7081 agccccatcc ttaggcctcc tccttcctag tctcctgata ttgggtctaa cccccacctc
     7141 ctgttaggca gattccttat ctggtgacac acccccattt cctggagcca tctctctcct
     7201 tgccagaacc tctaaggttt gcttacgatg gagccagaga ggatcctggg agggagagct
     7261 tggcaggggg tgggagggaa gggggggatg cgtgacctgc ccggttctca gtggccaccc
     7321 tgcgctaccc tctcccagaa cctgagctgc tctgacgcgg ctgtctggtg cgtttcactg
     7381 atcctggtgc tgcagcttcc ttacacttcc caagaggaga agcagtttgg aaaaacaaaa
     7441 tcagaataag ttggtcctga gttctaactt tggctcttca cctttctagt ccccaattta
     7501 tattgttcct ccgtgcgtca gttttacctg tgagataagg ccagtagcca gccccgtcct
     7561 ggcagggctg tggtgaggag gggggtgtcc gtgtggaaaa ctccctttgt gagaatggtg
     7621 cgtcctaggt gttcaccagg tcgtggccgc ctctactccc tttctctttc tccatccttc
     7681 tttccttaaa gagtccccag tgctatctgg gacatattcc tccgcccaga gcagggtccc
     7741 gcttccctaa ggccctgctc tgggcttctg ggtttgagtc cttggcaagc ccaggagagg
     7801 cgctcaggct tccctgtccc ccttcctcgt ccaccatctc atgcccctgg ctctcctgcc
     7861 ccttccctac aggggttcct ggctctgctc taagggcgaa ttcgcggccg ctaaattcaa
     7921 ttcgccctat agtgagtcgt attacaattc actggccgtc gttttacaac gtcgtgactg
     7981 ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagctg
     8041 gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctatacgt
     8101 acggcagttt aaggtttaca cctataaaag agagagccgt tatcgtctgt ttgtggatgt
     8161 acagagtgat attattgaca cgccggggcg acggatggtg atccccctgg ccagtgcacg
     8221 tctgctgtca gataaagtct cccgtgaact ttacccggtg gtgcatatcg gggatgaaag
     8281 ctggcgcatg atgaccaccg atatggccag tgtgccggtc tccgttatcg gggaagaagt
     8341 ggctgatctc agccaccgcg aaaatgacat caaaaacgcc attaacctga tgttctgggg
     8401 aatataaatg tcaggcatga gattatcaaa aaggatcttc acctagatcc ttttcacgta
     8461 gaaagccagt ccgcagaaac ggtgctgacc ccggatgaat gtcagctact gggctatctg
     8521 gacaagggaa aacgcaagcg caaagagaaa gcaggtagct tgcagtgggc ttacatggcg
     8581 atagctagac tgggcggttt tatggacagc aagcgaaccg gaattgccag ctggggcgcc
     8641 ctctggtaag gttgggaagc cctgcaaagt aaactggatg gctttcttgc cgccaaggat
     8701 ctgatggcgc aggggatcaa gctctgatca agagacagga tgaggatcgt ttcgcatgat
     8761 tgaacaagat ggattgcacg caggttctcc ggccgcttgg gtggagaggc tattcggcta
     8821 tgactgggca caacagacaa tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca
     8881 ggggcgcccg gttctttttg tcaagaccga cctgtccggt gccctgaatg aactgcaaga
     8941 cgaggcagcg cggctatcgt ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga
     9001 cgttgtcact gaagcgggaa gggactggct gctattgggc gaagtgccgg ggcaggatct
     9061 cctgtcatct caccttgctc ctgccgagaa agtatccatc atggctgatg caatgcggcg
     9121 gctgcatacg cttgatccgg ctacctgccc attcgaccac caagcgaaac atcgcatcga
     9181 gcgagcacgt actcggatgg aagccggtct tgtcgatcag gatgatctgg acgaagagca
     9241 tcaggggctc gcgccagccg aactgttcgc caggctcaag gcgagcatgc ccgacggcga
     9301 ggatctcgtc gtgacccatg catggcgatg cctgcttgcc gaatatcatg gtggaaaatg
     9361 gccgcttttc tggattcatc gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca
     9421 tagcgttggc tacccgtgat attgctgaag agcttggcgg cgaatgggct gaccgcttcc
     9481 tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat cgccttctat cgccttcttg
     9541 acgagttctt ctgaattatt aacgcttaca atttcctgat gcggtatttt ctccttacgc
     9601 atctgtgcgg tatttcacac cgcatcaggt ggcacttttc ggggaaatgt gcgcggaacc
     9661 cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag attatcaaaa
     9721 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata
     9781 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg
     9841 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata
     9901 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg
     9961 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct
    10021 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt
    10081 tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc
    10141 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga
    10201 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt
    10261 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc
    10321 atgccatccg taagatgctt ttctgtgact ggtgaatact caaccaagtc attctgagaa
    10381 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca
    10441 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca
    10501 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct
    10561 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc
    10621 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa
    10681 tattattgaa gcatttatca gggttattgt ctcatgacca aaatccctta acgtgagttt
    10741 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt
    10801 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt
    10861 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag
    10921 ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta
    10981 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat
    11041 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg
    11101 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg
    11161 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac
    11221 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga
    11281 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt
    11341 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta
    11401 cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat
    11461 tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg
    11521 accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct
    11581 ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa
    11641 gcgggcagtg agcgcaacgc aattaatgtg agttagctca ctcattaggc accccaggct
    11701 ttacacttta tgcttccggc tcgtatgttg tgtggaattg tgagcggata acaatttcac
    11761 acaggaaaca gctatgacca tgattacgcc aagctcagaa ttaaccctca ctaaaggga
//
