LOCUS       sequence-327867-        8758 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of HLA-A*01:01 cDNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8758)
  AUTHORS   Lee MN, Meyerson M
  TITLE     Antigen identification for HLA class I- and HLA class II-restricted 
            T cell receptors using cytokine-capturing antigen-presenting cells.
  JOURNAL   Sci Immunol. 2021 Jan 22;6(55). pii: 6/55/eabf4001. doi: 
            10.1126/sciimmunol.abf4001.
  PUBMED    33483338
REFERENCE   2  (bases 1 to 8758)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci 
            Immunol."; date: "2021-01-22"; volume: "6(55). pii: 6/55/eabf4001. 
            doi"; pages: " 10.1126/sciimmunol.abf4001"
COMMENT     Sequence Label: HLA_A0101
FEATURES             Location/Qualifiers
     source          1..8758
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        65..368
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        369..572
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     523..543
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      606..615
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    1765..2353
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(1818..1838)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2236..2247)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             2425..2658
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    2736..2857
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2773..2792)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2827..2846
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      2897..3032
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(3052..3071)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3053..3071)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3081..3098)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3081..3097)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3090..3112)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      3239..3694
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3326..3345)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3536..3557
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        3720..3824
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3825..3893,3894..4685)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4043..4062)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4856..5444
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5345..5364
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5598..5615
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    5732..5753
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(5768..5773,5774..5791,5792..5798)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5806..5822
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5811..5833
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     5830..5846
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5830..5846
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     5865..5885
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        5867..5885
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        5913..6139
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             6140..6320
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    6367..6492
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    6985..7218
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             7403..7447
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        7622..8122
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     8041..8059
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             8134..8529
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     misc_feature    8589..8706
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 cccggggtta ttaatagtaa tcaattacgg ggtcattagt tcatagccca tatatggagt
       61 tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc
      121 cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac
      181 gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata
      241 tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc
      301 agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta
      361 ttaccatggt gatgcggttt tggcagtaca tcaatgggcg tggatagcgg tttgactcac
      421 ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttgg caccaaaatc
      481 aacgggactt tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc
      541 gtgtacggtg ggaggtctat ataagcagag ctctctggct aactgtcggg atcaacaagt
      601 ttaccgccac catggccgtc atggcgcccc gaaccctcct cctgctactc tcgggggccc
      661 tggccctgac ccagacctgg gcgggctccc actccatgag gtatttcttc acatccgtgt
      721 cccggcccgg ccgcggggag ccccgcttca tcgccgtggg ctacgtggac gacacgcagt
      781 tcgtgcggtt cgacagcgac gccgcgagcc agaagatgga gccgcgggcg ccgtggatag
      841 agcaggaggg gccggagtat tgggaccagg agacacggaa tatgaaggcc cactcacaga
      901 ctgaccgagc gaacctgggg accctgcgcg gctactacaa ccagagcgag gacggttctc
      961 acaccatcca gataatgtat ggctgcgacg tggggccgga cgggcgcttc ctccgcgggt
     1021 accggcagga cgcctacgac ggcaaggatt acatcgccct gaacgaggac ctgcgctctt
     1081 ggaccgcggc ggacatggca gctcagatca ccaagcgcaa gtgggaggcg gtccatgcgg
     1141 cggagcagcg gagagtctac ctggagggcc ggtgcgtgga cgggctccgc agatacctgg
     1201 agaacgggaa ggagacgctg cagcgcacgg acccccccaa gacacatatg acccaccacc
     1261 ccatctctga ccatgaggcc accctgaggt gctgggccct gggcttctac cctgcggaga
     1321 tcacactgac ctggcagcgg gatggggagg accagaccca ggacacggag ctcgtggaga
     1381 ccaggcctgc aggggatgga accttccaga agtgggcggc tgtggtggtg ccttctggag
     1441 aggagcagag atacacctgc catgtgcagc atgagggtct gcccaagccc ctcaccctga
     1501 gatgggagct gtcttcccag cccaccatcc ccatcgtggg catcattgct ggcctggttc
     1561 tccttggagc tgtgatcact ggagctgtgg tcgctgccgt gatgtggagg aggaagagct
     1621 cagatagaaa aggagggagt tacactcagg ctgcaagcag tgacagtgcc cagggctctg
     1681 atgtgtctct cacagcttgt aaagtgtgat gtacaaagtg gtttagtaat gagctagcgc
     1741 taaccggtgg cgcgttaagt cgacaatcaa cctctggatt acaaaatttg tgaaagattg
     1801 actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct
     1861 ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg
     1921 ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact
     1981 gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc
     2041 gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc
     2101 cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaaa
     2161 tcatcgtcct ttccttggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc
     2221 ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg
     2281 gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg
     2341 gccgcctccc cgcgtcgact ttaagaccaa tgacttacaa ggcagctgta gatcttagcc
     2401 actttttaaa agaaaagggg ggactggaag ggctaattca ctcccaacga agacaagatc
     2461 tgctttttgc ttgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg
     2521 gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag
     2581 tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag
     2641 tgtggaaaat ctctagcagt acgtatagta gttcatgtca tcttattatt cagtatttat
     2701 aacttgcaaa gaaatgaata tcagagagtg agaggaactt gtttattgca gcttataatg
     2761 gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt
     2821 ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggctc tagctatccc
     2881 gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca
     2941 tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt
     3001 ccagaagtag tgaggaggct tttttggagg cctagggacg tacccaattc gccctatagt
     3061 gagtcgtatt acgcgcgctc actggccgtc gttttacaac gtcgtgactg ggaaaaccct
     3121 ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagctg gcgtaatagc
     3181 gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgaatgggac
     3241 gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct
     3301 acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg
     3361 ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt
     3421 gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca
     3481 tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga
     3541 ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa
     3601 gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac
     3661 gcgaatttta acaaaatatt aacgcttaca atttaggtgg cacttttcgg ggaaatgtgc
     3721 gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac
     3781 aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt
     3841 tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag
     3901 aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg
     3961 aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa
     4021 tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc
     4081 aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag
     4141 tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa
     4201 ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc
     4261 taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg
     4321 agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa
     4381 caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa
     4441 tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg
     4501 gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag
     4561 cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg
     4621 caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt
     4681 ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt
     4741 aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac
     4801 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
     4861 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
     4921 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
     4981 gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga
     5041 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
     5101 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
     5161 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
     5221 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
     5281 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
     5341 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
     5401 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
     5461 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat
     5521 cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca
     5581 gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca
     5641 aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac aggtttcccg
     5701 actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact cattaggcac
     5761 cccaggcttt acactttatg cttccggctc gtatgttgtg tggaattgtg agcggataac
     5821 aatttcacac aggaaacagc tatgaccatg attacgccaa gcgcgcaatt aaccctcact
     5881 aaagggaaca aaagctggag ctgcaagctt aatgtagtct tatgcaatac tcttgtagtc
     5941 ttgcaacatg gtaacgatga gttagcaaca tgccttacaa ggagagaaaa agcaccgtgc
     6001 atgccgattg gtggaagtaa ggtggtacga tcgtgcctta ttaggaaggc aacagacggg
     6061 tctgacatgg attggacgaa ccactgaatt gccgcattgc agagatattg tatttaagtg
     6121 cctagctcga tacataaacg ggtctctctg gttagaccag atctgagcct gggagctctc
     6181 tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt
     6241 agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc
     6301 agtgtggaaa atctctagca gtggcgcccg aacagggact tgaaagcgaa agggaaacca
     6361 gaggagctct ctcgacgcag gactcggctt gctgaagcgc gcacggcaag aggcgagggg
     6421 cggcgactgg tgagtacgcc aaaaattttg actagcggag gctagaagga gagagatggg
     6481 tgcgagagcg tcagtattaa gcgggggaga attagatcgc gatgggaaaa aattcggtta
     6541 aggccagggg gaaagaaaaa atataaatta aaacatatag tatgggcaag cagggagcta
     6601 gaacgattcg cagttaatcc tggcctgtta gaaacatcag aaggctgtag acaaatactg
     6661 ggacagctac aaccatccct tcagacagga tcagaagaac ttagatcatt atataataca
     6721 gtagcaaccc tctattgtgt gcatcaaagg atagagataa aagacaccaa ggaagcttta
     6781 gacaagatag aggaagagca aaacaaaagt aagaccaccg cacagcaagc ggccgctgat
     6841 cttcagacct ggaggaggag atatgaggga caattggaga agtgaattat ataaatataa
     6901 agtagtaaaa attgaaccat taggagtagc acccaccaag gcaaagagaa gagtggtgca
     6961 gagagaaaaa agagcagtgg gaataggagc tttgttcctt gggttcttgg gagcagcagg
     7021 aagcactatg ggcgcagcgt caatgacgct gacggtacag gccagacaat tattgtctgg
     7081 tatagtgcag cagcagaaca atttgctgag ggctattgag gcgcaacagc atctgttgca
     7141 actcacagtc tggggcatca agcagctcca ggcaagaatc ctggctgtgg aaagatacct
     7201 aaaggatcaa cagctcctgg ggatttgggg ttgctctgga aaactcattt gcaccactgc
     7261 tgtgccttgg aatgctagtt ggagtaataa atctctggaa cagatttgga atcacacgac
     7321 ctggatggag tgggacagag aaattaacaa ttacacaagc ttaatacact ccttaattga
     7381 agaatcgcaa aaccagcaag aaaagaatga acaagaatta ttggaattag ataaatgggc
     7441 aagtttgtgg aattggttta acataacaaa ttggctgtgg tatataaaat tattcataat
     7501 gatagtagga ggcttggtag gtttaagaat agtttttgct gtactttcta tagtgaatag
     7561 agttaggcag ggatattcac cattatcgtt tcagacccac ctcccaaccc cgaggggacc
     7621 cttgcgcctt ttccaaggca gccctgggtt tgcgcaggga cgcggctgct ctgggcgtgg
     7681 ttccgggaaa cgcagcggcg ccgaccctgg gtctcgcaca ttcttcacgt ccgttcgcag
     7741 cgtcacccgg atcttcgccg ctacccttgt gggccccccg gcgacgcttc ctgctccgcc
     7801 cctaagtcgg gaaggttcct tgcggttcgc ggcgtgccgg acgtgacaaa cggaagccgc
     7861 acgtctcact agtaccctcg cagacggaca gcgccaggga gcaatggcag cgcgccgacc
     7921 gcgatgggct gtggccaata gcggctgctc agcagggcgc gccgagagca gcggccggga
     7981 aggggcggtg cgggaggcgg ggtgtggggc ggtagtgtgg gccctgttcc tgcccgcgcg
     8041 gtgttccgca ttctgcaagc ctccggagcg cacgtcggca gtcggctccc tcgttgaccg
     8101 aatcaccgac ctctctcccc agggggtacc accatggcca agcctttgtc tcaagaagaa
     8161 tccaccctca ttgaaagagc aacggctaca atcaacagca tccccatctc tgaagactac
     8221 agcgtcgcca gcgcagctct ctctagcgac ggccgcatct tcactggtgt caatgtatat
     8281 cattttactg ggggaccttg tgcagaactc gtggtgctgg gcactgctgc tgctgcggca
     8341 gctggcaacc tgacttgtat cgtcgcgatc ggaaatgaga acaggggcat cttgagcccc
     8401 tgcggacggt gccgacaggt gcttctcgat ctgcatcctg ggatcaaagc catagtgaag
     8461 gacagtgatg gacagccgac ggcagttggg attcgtgaat tgctgccctc tggttatgtg
     8521 tgggagggcc tgcagctgca gtagtaagaa ttctagatct tgagacaaat ggcagtattc
     8581 atccacaatt ttaaaagaaa aggggggatt ggggggtaca gtgcagggga aagaatagta
     8641 gacataatag caacagacat acaaactaaa gaattacaaa aacaaattac aaaaattcaa
     8701 aattttcggg tttattacag ggacagcaga gatccacttt ggcgccggct cgaggggg
//
