LOCUS       sequence-327868-        8672 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of candidate TCR #6 TCRalpha cDNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8672)
  AUTHORS   Lee MN, Meyerson M
  TITLE     Antigen identification for HLA class I- and HLA class II-restricted 
            T cell receptors using cytokine-capturing antigen-presenting cells.
  JOURNAL   Sci Immunol. 2021 Jan 22;6(55). pii: 6/55/eabf4001. doi: 
            10.1126/sciimmunol.abf4001.
  PUBMED    33483338
REFERENCE   2  (bases 1 to 8672)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci 
            Immunol."; date: "2021-01-22"; volume: "6(55). pii: 6/55/eabf4001. 
            doi"; pages: " 10.1126/sciimmunol.abf4001"
COMMENT     Sequence Label: CMVTCRa_candidate6
FEATURES             Location/Qualifiers
     source          1..8672
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        65..368
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        369..572
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     523..543
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      608..617
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    1483..2071
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(1536..1556)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(1954..1965)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             2143..2376
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    2454..2575
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2491..2510)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2545..2564
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      2615..2750
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(2770..2789)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2771..2789)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2799..2816)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2799..2815)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2808..2830)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      2957..3412
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3044..3063)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3254..3275
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        3438..3542
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3543..3611,3612..4403)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3761..3780)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4574..5162
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5063..5082
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5316..5333
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    5450..5471
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(5486..5491,5492..5509,5510..5516)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5524..5540
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5529..5551
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     5548..5564
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5548..5564
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     5583..5603
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        5585..5603
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        5631..5857
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             5858..6038
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    6085..6210
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    6703..6936
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             7121..7165
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        7340..7840
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     7759..7777
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             7862..8461
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(7862..7881)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     8358..8378
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    8503..8620
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 cccggggtta ttaatagtaa tcaattacgg ggtcattagt tcatagccca tatatggagt
       61 tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc
      121 cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac
      181 gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata
      241 tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc
      301 agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta
      361 ttaccatggt gatgcggttt tggcagtaca tcaatgggcg tggatagcgg tttgactcac
      421 ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttgg caccaaaatc
      481 aacgggactt tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc
      541 gtgtacggtg ggaggtctat ataagcagag ctctctggct aactgtcggg atcaacaagt
      601 ttgtgccgcc accatggaga ccctcttggg cctgcttatc ctttggctgc agctgcaatg
      661 ggtgagcagc aaacaggagg tgacgcagat tcctgcagct ctgagtgtcc cagaaggaga
      721 aaacttggtt ctcaactgca gtttcactga tagcgctatt tacaacctcc agtggtttag
      781 gcaggaccct gggaaaggtc tcacatctct gttgcttatt cagtcaagtc agagagagca
      841 aacaagtgga agacttaatg cctcgctgga taaatcatca ggacgtagta ctttatacat
      901 tgcagcttct cagcctggtg actcagccac ctacctctgt gctgtgaggt ctgggggtta
      961 ccagaaagtt acctttggaa ctggaacaaa gctccaagtc atcccaaata tccagaaccc
     1021 tgaccctgcc gtgtaccagc tgagagactc taaatccagt gacaagtctg tctgcctatt
     1081 caccgatttt gattctcaaa caaatgtgtc acaaagtaag gattctgatg tgtatatcac
     1141 agacaaatgt gtgctagaca tgaggtctat ggattttaaa tctaattcag cagtagcctg
     1201 gagcaacaaa tctgactttg catgtgcaaa cgccttcaac aacagcatta ttccagaaga
     1261 caccttcttc cccagcccag aaagttcctg tgatgtcaag ctggtcgaga aaagctttga
     1321 aacagatacg aacctaaact ttcaaaacct gtcagtgatt gggttccgaa tcctcctcct
     1381 gaaagtggcc gggtttaatc tgctcatgac gctgcggctg tggtccagct agaaagtggt
     1441 ttagtaatga gctagcgctt accggtggcg cgttaagtcg acaatcaacc tctggattac
     1501 aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga
     1561 tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc
     1621 tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa
     1681 cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg cattgccacc
     1741 acctgtcagc tcctttccgg gactttcgct ttccccctcc ctattgccac ggcggaactc
     1801 atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc
     1861 gtggtgttgt cggggaaatc atcgtccttt ccttggctgc tcgcctgtgt tgccacctgg
     1921 attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc ggaccttcct
     1981 tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg
     2041 agtcggatct ccctttgggc cgcctccccg cgtcgacttt aagaccaatg acttacaagg
     2101 cagctgtaga tcttagccac tttttaaaag aaaagggggg actggaaggg ctaattcact
     2161 cccaacgaag acaagatctg ctttttgctt gtactgggtc tctctggtta gaccagatct
     2221 gagcctggga gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc
     2281 cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc
     2341 tcagaccctt ttagtcagtg tggaaaatct ctagcagtac gtatagtagt tcatgtcatc
     2401 ttattattca gtatttataa cttgcaaaga aatgaatatc agagagtgag aggaacttgt
     2461 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag
     2521 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg
     2581 tctggctcta gctatcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc
     2641 cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc
     2701 ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc tagggacgta
     2761 cccaattcgc cctatagtga gtcgtattac gcgcgctcac tggccgtcgt tttacaacgt
     2821 cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc
     2881 gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc
     2941 ctgaatggcg aatgggacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt
     3001 acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc
     3061 ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct
     3121 ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat
     3181 ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc
     3241 acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc
     3301 tattcttttg atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg
     3361 atttaacaaa aatttaacgc gaattttaac aaaatattaa cgcttacaat ttaggtggca
     3421 cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata
     3481 tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga
     3541 gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc
     3601 ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg
     3661 cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc
     3721 ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat
     3781 cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact
     3841 tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat
     3901 tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga
     3961 tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc
     4021 ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga
     4081 tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag
     4141 cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc
     4201 gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt
     4261 ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct
     4321 acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg
     4381 cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg
     4441 atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca
     4501 tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga
     4561 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
     4621 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
     4681 aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt
     4741 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
     4801 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
     4861 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
     4921 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
     4981 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
     5041 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
     5101 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
     5161 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     5221 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
     5281 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
     5341 aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct
     5401 ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt
     5461 agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg
     5521 gaattgtgag cggataacaa tttcacacag gaaacagcta tgaccatgat tacgccaagc
     5581 gcgcaattaa ccctcactaa agggaacaaa agctggagct gcaagcttaa tgtagtctta
     5641 tgcaatactc ttgtagtctt gcaacatggt aacgatgagt tagcaacatg ccttacaagg
     5701 agagaaaaag caccgtgcat gccgattggt ggaagtaagg tggtacgatc gtgccttatt
     5761 aggaaggcaa cagacgggtc tgacatggat tggacgaacc actgaattgc cgcattgcag
     5821 agatattgta tttaagtgcc tagctcgata cataaacggg tctctctggt tagaccagat
     5881 ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt
     5941 gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc
     6001 cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa cagggacttg
     6061 aaagcgaaag ggaaaccaga ggagctctct cgacgcagga ctcggcttgc tgaagcgcgc
     6121 acggcaagag gcgaggggcg gcgactggtg agtacgccaa aaattttgac tagcggaggc
     6181 tagaaggaga gagatgggtg cgagagcgtc agtattaagc gggggagaat tagatcgcga
     6241 tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat ataaattaaa acatatagta
     6301 tgggcaagca gggagctaga acgattcgca gttaatcctg gcctgttaga aacatcagaa
     6361 ggctgtagac aaatactggg acagctacaa ccatcccttc agacaggatc agaagaactt
     6421 agatcattat ataatacagt agcaaccctc tattgtgtgc atcaaaggat agagataaaa
     6481 gacaccaagg aagctttaga caagatagag gaagagcaaa acaaaagtaa gaccaccgca
     6541 cagcaagcgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     6601 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     6661 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     6721 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     6781 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     6841 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     6901 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     6961 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     7021 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     7081 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     7141 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     7201 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     7261 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     7321 cccaaccccg aggggaccct tgcgcctttt ccaaggcagc cctgggtttg cgcagggacg
     7381 cggctgctct gggcgtggtt ccgggaaacg cagcggcgcc gaccctgggt ctcgcacatt
     7441 cttcacgtcc gttcgcagcg tcacccggat cttcgccgct acccttgtgg gccccccggc
     7501 gacgcttcct gctccgcccc taagtcggga aggttccttg cggttcgcgg cgtgccggac
     7561 gtgacaaacg gaagccgcac gtctcactag taccctcgca gacggacagc gccagggagc
     7621 aatggcagcg cgccgaccgc gatgggctgt ggccaatagc ggctgctcag cagggcgcgc
     7681 cgagagcagc ggccgggaag gggcggtgcg ggaggcgggg tgtggggcgg tagtgtgggc
     7741 cctgttcctg cccgcgcggt gttccgcatt ctgcaagcct ccggagcgca cgtcggcagt
     7801 cggctccctc gttgaccgaa tcaccgacct ctctccccag ggggtaccac cggagcttac
     7861 catgaccgag tacaagccca cggtgcgcct cgccacccgc gacgacgtcc ccagggccgt
     7921 acgcaccctc gccgccgcgt tcgccgacta ccccgccacg cgccacaccg tcgatccgga
     7981 ccgccacatc gagcgggtca ccgagctgca agaactcttc ctcacgcgcg tcgggctcga
     8041 catcggcaag gtgtgggtcg cggacgacgg cgccgccgtg gcggtctgga ccacgccgga
     8101 gagcgtcgaa gcgggggcgg tgttcgccga gatcggcccg cgcatggccg agttgagcgg
     8161 ttcccggctg gccgcgcagc aacagatgga aggcctcctg gcgccgcacc ggcccaagga
     8221 gcccgcgtgg ttcctggcca ccgtcggcgt ctcgcccgac caccagggca agggtctggg
     8281 cagcgccgtc gtgctccccg gagtggaggc ggccgagcgc gccggggtgc ccgccttcct
     8341 ggagacctcc gcgccccgca acctcccctt ctacgagcgg ctcggcttca ccgtcaccgc
     8401 cgacgtcgag gtgcccgaag gaccgcgcac ctggtgcatg acccgcaagc ccggtgcctg
     8461 agaattctag atcttgagac aaatggcagt attcatccac aattttaaaa gaaaaggggg
     8521 gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag acatacaaac
     8581 taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt acagggacag
     8641 cagagatcca ctttggcgcc ggctcgaggg gg
//
