LOCUS       sequence-327883-        8099 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  CRISPR‐Cas9 tool for the genetic engineering of Bacillus phages.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8099)
  AUTHORS   Otte K, Kuhne NM, Furrer AD, Baena Lozada LP, Lutz VT, Schilling T, 
            Hertel R
  TITLE     A CRISPR-Cas9 tool to explore the genetics of Bacillus subtilis 
            phages.
  JOURNAL   Lett Appl Microbiol. 2020 Jul 2. doi: 10.1111/lam.13349.
  PUBMED    32615024
REFERENCE   2  (bases 1 to 8099)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Lett Appl 
            Microbiol. 2020 Jul 2. doi: 10.1111/lam.13349."
COMMENT     Sequence Label: pRH030
FEATURES             Location/Qualifiers
     source          1..8099
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..546
                     /direction=RIGHT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     447..466
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     misc_RNA        complement(774..852)
                     /label=tracrRNA
                     /note="trans-activating CRISPR RNA for the Streptococcus 
                     pyogenes CRISPR/Cas9 system"
     CDS             1148..5254
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             1148..5251
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     primer_bind     complement(5404..5423)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5405..5423)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     terminator      complement(5460..5494)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     misc_RNA        complement(5516..5591)
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     protein_bind    5665..5686
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(5701..5706,5707..5724,5725..5731)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5739..5755
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5744..5766
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     5763..5779
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5763..5779
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5795..5812)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5795..5811)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5804..5826)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 ttgagatcgt tttggtctgc gcgtaatctc ttgctctgaa aacgaaaaaa ccgccttgca
       61 gggcggtttt tcgaaggttc tctgagctac caactctttg aaccgaggta actggcttgg
      121 aggagcgcag tcaccaaaac ttgtcctttc agtttagcct taaccggcgc atgacttcaa
      181 gactaactcc tctaaatcaa ttaccagtgg ctgctgccag tggtgctttt gcatgtcttt
      241 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcggac tgaacggggg
      301 gttcgtgcat acagtccagc ttggagcgaa ctgcctaccc ggaactgagt gtcaggcgtg
      361 gaatgagaca aacgcggcca taacagcgga atgacaccgg taaaccgaaa ggcaggaaca
      421 ggagagcgca cgagggagcc gccaggggga aacgcctggt atctttatag tcctgtcggg
      481 tttcgccacc actgatttga gcgtcagatt tcgtgatgct tgtcaggggg gcggagccta
      541 tggaaaaacg gctttgccgc ggccctctca cttccctgtt aagtgtacag ggcctcttgc
      601 gggattacga aatcatcctg tggagcttag taggtttagc aagatggcag cgcctaaatg
      661 tagaatgata aaaggattaa gagattaatt tccctaaaaa tgataaaaca agcgttttga
      721 aagcgcttgt ttttttggtt tgcagtcaga gtagaataga agtatcaaaa aaagcaccga
      781 ctcggtgcca ctttttcaag ttgataacgg actagcctta ttttaacttg ctatgctgtt
      841 ttgaatggtt ccaacaagat tattttataa cttttataac aaataatcaa ggagaaattc
      901 aaagaaattt atcagccata aaacaatact taatactata gaatgataac aaaataaact
      961 actttttaaa agaattttgt gttataatct atttattatt aagtattggg taatattttt
     1021 tgaagagata ttttgaaaaa gaaaaattaa agcatattaa actaatttcg gaggtcatta
     1081 aaactattat tgaaatcatc aaactcatta tggatttaat ttaaactttt tattttagga
     1141 ggcaaaaatg gataagaaat actcaatagg cttagatatc ggcacaaata gcgtcggatg
     1201 ggcggtgatc actgatgaat ataaggttcc gtctaaaaag ttcaaggttc tgggaaatac
     1261 agaccgccac agtatcaaaa aaaatcttat aggggctctt ttatttgaca gtggagagac
     1321 agcggaagcg actcgtctca aacggacagc tcgtagaagg tatacacgtc ggaagaatcg
     1381 tatttgttat ctacaggaga ttttttcaaa tgagatggcg aaagtagatg atagtttctt
     1441 tcatcgactt gaagagtctt ttttggtgga agaagacaag aagcatgaac gtcatcctat
     1501 ttttggaaat atagtagatg aagttgctta tcatgagaaa tatccaacta tctatcatct
     1561 gcgaaaaaaa ttggtagatt ctactgataa agcggatttg cgcttaatct atttggcctt
     1621 agcgcatatg attaagtttc gtggtcattt tttgattgag ggagatttaa atcctgataa
     1681 tagtgatgtg gacaaactat ttatccagtt ggtacaaacc tacaatcaat tatttgaaga
     1741 aaaccctatt aacgcaagtg gagtagatgc taaagcgatt ctttctgcac gattgagtaa
     1801 atcaagacga ttagaaaatc tcattgctca gctccccggt gagaagaaaa atggcttatt
     1861 tgggaatctc attgctttgt cattgggttt gacccctaat tttaaatcaa attttgattt
     1921 ggcagaagat gctaaattac agctttcaaa agatacttac gatgatgatt tagataattt
     1981 attggcgcaa attggagatc aatatgctga tttgtttttg gcagctaaga atttatcaga
     2041 tgctatttta ctttcagata tcctaagagt aaatactgaa ataactaagg ctcccctatc
     2101 agcttcaatg attaaacgct acgatgaaca tcatcaagac ttgactcttt taaaagcttt
     2161 agttcgacaa caacttccag aaaagtataa agaaatcttt tttgatcaat caaaaaacgg
     2221 atatgcaggt tatattgatg ggggagctag ccaagaagaa ttttataaat ttatcaaacc
     2281 aattttagaa aaaatggatg gtactgagga attattggtg aaactaaatc gtgaagattt
     2341 gctgcgcaag caacggacct ttgacaacgg ctctattccc catcaaattc acttgggtga
     2401 gctgcatgct attttgagaa gacaagaaga cttttatcca tttttaaaag acaatcgtga
     2461 gaagattgaa aaaatcttga cttttcgaat tccttattat gttggtccat tggcgcgtgg
     2521 caatagtcgt tttgcatgga tgactcggaa gtctgaagaa acaattaccc catggaattt
     2581 tgaagaagtt gtcgataaag gtgcttcagc tcaatcattt attgaacgca tgacaaactt
     2641 tgataaaaat cttccaaatg aaaaagtact accaaaacat agtttgcttt atgagtattt
     2701 tacggtttat aacgaattga caaaggtcaa atatgttact gaaggaatgc gaaaaccagc
     2761 atttctttca ggtgaacaga agaaagccat tgttgattta ctcttcaaaa caaatcgaaa
     2821 agtaaccgtt aagcaattaa aagaagatta tttcaaaaaa atagaatgtt ttgatagtgt
     2881 tgaaatttca ggagttgaag atagatttaa tgcttcatta ggtacctacc atgatttgct
     2941 aaaaattatt aaagataaag attttttgga taatgaagaa aatgaagata tcttagagga
     3001 tattgtttta acattgacct tatttgaaga tagggagatg attgaggaaa gacttaaaac
     3061 atatgctcac ctctttgatg ataaggtgat gaaacagctt aaacgtcgcc gttatactgg
     3121 ttggggacgt ttgtctcgaa aattgattaa tggtattagg gataagcaat ctggcaaaac
     3181 aatattagat tttttgaaat cagatggttt tgccaatcgc aattttatgc agctgatcca
     3241 tgatgatagt ttgacattta aagaagacat tcaaaaagca caagtgtctg gacaaggcga
     3301 tagtttacat gaacatattg caaatttagc tggtagccct gctattaaaa aaggtatttt
     3361 acagactgta aaagttgttg atgaattggt caaagtaatg gggcggcata agccagaaaa
     3421 tatcgttatt gaaatggcac gtgaaaatca gacaactcaa aagggccaga aaaattcgcg
     3481 agagcgtatg aaacgaatcg aagaaggtat caaagaatta ggaagtcaga ttcttaaaga
     3541 gcatcctgtt gaaaatactc aattgcaaaa tgaaaagctc tatctctatt atctccaaaa
     3601 tggaagagac atgtatgtgg accaagaatt agatattaat cgtttaagtg attatgatgt
     3661 cgatcacatt gttccacaaa gtttccttaa agacgattca atagacaata aggtcttaac
     3721 gcgttctgat aaaaatcgtg gtaaatcgga taacgttcca agtgaagaag tagtcaaaaa
     3781 gatgaaaaac tattggagac aacttctaaa cgccaagtta atcactcaac gtaagtttga
     3841 taatttaacg aaagctgaac gtggaggttt gagtgaactt gataaagctg gttttatcaa
     3901 acgccaattg gttgaaactc gccaaatcac taagcatgtg gcacaaattt tggatagtcg
     3961 catgaatact aaatacgatg aaaatgataa acttattcga gaggttaaag tgattacctt
     4021 aaaatctaaa ttagtttctg acttccgaaa agatttccaa ttctataaag tacgtgagat
     4081 taacaattac catcatgccc atgatgcgta tctaaatgcc gtcgttggaa ctgctttgat
     4141 taagaaatat ccaaaacttg aatcggagtt tgtctatggt gattataaag tttatgatgt
     4201 tcgtaaaatg attgctaagt ctgagcaaga aataggcaaa gcaaccgcaa aatatttctt
     4261 ttactctaat atcatgaact tcttcaaaac agaaattaca cttgcaaatg gagagattcg
     4321 caaacgccct ctaatcgaaa ctaatgggga aactggagaa attgtctggg ataaagggcg
     4381 agattttgcc acagtgcgca aagtattgtc catgccccaa gtcaatattg tcaagaaaac
     4441 agaagtacag acaggcggat tctccaagga gtcaatttta ccaaaaagaa attcggacaa
     4501 gcttattgct cgtaaaaaag actgggatcc aaaaaaatat ggtggttttg atagtccaac
     4561 ggtagcttat tcagtcctag tggttgctaa ggtggaaaaa gggaaatcga agaagttaaa
     4621 atccgttaaa gagttactag ggatcacaat tatggaaaga agttcctttg aaaaaaatcc
     4681 gattgacttt ttagaagcta aaggatataa ggaagttaaa aaagacttaa tcattaaact
     4741 acctaaatat agtctttttg agttagaaaa cggtcgtaaa cggatgctgg ctagtgccgg
     4801 agaattacaa aaaggaaatg agctggctct gccaagcaaa tatgtgaatt ttttatattt
     4861 agctagtcat tatgaaaagt tgaagggtag tccagaagat aacgaacaaa aacaattgtt
     4921 tgtggagcag cataagcatt atttagatga gattattgag caaatcagtg aattttctaa
     4981 gcgtgttatt ttagcagatg ccaatttaga taaagttctt agtgcatata acaaacatag
     5041 agacaaacca atacgtgaac aagcagaaaa tattattcat ttatttacgt tgacgaatct
     5101 tggagctccc gctgctttta aatattttga tacaacaatt gatcgtaaac gatatacgtc
     5161 tacaaaagaa gttttagatg ccactcttat ccatcaatcc atcactggtc tttatgaaac
     5221 acgcattgat ttgagtcagc taggaggtga ctgaagtata ttttagatga agattatttc
     5281 ttaatctaga gcgcttcttg cttacccggc gggagcgctt tttctttttc ttggccttat
     5341 tggcccgggc ctcgttggcc aaacgcaaag aaccctcccg gcacatttgc cgagaggggt
     5401 cgaccctata gtgagtcgta ttaaaaaggc ccagtctttc gactgggcct aagctttaga
     5461 taaaaaacgc ccggcggcaa ccgagcgttc tgaacaaatc cagatggagt aaaaagcacc
     5521 gactcggtgc cactttttca agttgataac ggactagcct tattttaact tgctatttct
     5581 agctctaaaa ctgagaccga ttcattaatg cagctggcac gacaggtttc ccgactggaa
     5641 agcgggcagt gagcgcaacg caattaatgt gagttagctc actcattagg caccccaggc
     5701 tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca
     5761 cacaggaaac agctatgacc atgattacga attcactggc cgtcgtttta caacgtcgtg
     5821 actgggaaaa ccctggcgtt acccaactta atcgccttgc agcacatccc cctttcgcca
     5881 gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc ccaacagttg cgcagcctga
     5941 atggcgaatg aggtctcgcg taggtacatt ttactcaatt ctctaatcac ggtcaagaat
     6001 tcgcatcaca cgcaaaaagg aaattggaat aaatgcgaaa tttgagatgt taattaaaga
     6061 cctttttgag gtcttttttt cttagatttt tggggttatt taggggagaa aacatagggg
     6121 ggtactacga cctcccccct aggtgtccat tgtccattgt ccaaacaaat aaataaatat
     6181 tgggttttta atgttaaaag gttgtttttt atgttaaagt gaaaaaaaca gatgttggga
     6241 ggtacagtga tggttgtaga tagaaaagaa gagaaaaaag ttgctgttac tttaagactt
     6301 acaacagaag aaaatgagat attaaataga atcaaagaaa aatataatat tagcaaatca
     6361 gatgcaaccg gtattctaat aaaaaaatat gcaaaggagg aatacggtgc attttaaaca
     6421 aaaaaagata gacagcactg gcatgctgcc tatctatgac taaattttgt taaatgtatt
     6481 agcaccgtta ttatatcatg agcgaaaatg taataaaaga aactgaaaac aagaaaaatt
     6541 caagaggacg taattggaca tttgttttat atccagaatc agcaaaagcc gagtggttag
     6601 agtatttaaa agagttacac attcaatttg tagtgtctcc attacatgat agggatactg
     6661 atacagaaga taggatgaaa aaagagcatt atcatattct agtgatgtat gagggtaata
     6721 aatcttatga acagataaaa ataattacag aagaattgaa tgcgactatt ccgcagattg
     6781 caggaagtgt gaaaggtctt gtgagatata tgcttcacat ggacgatcct aataaattta
     6841 aatatcaaaa agaagatatg atagtttatg gcggtgtaga tgttgatgaa ttattaaaga
     6901 aaacaacaac agatagatat aaattaatta aagaaatgat tgagtttatt gatgaacaag
     6961 gaatcgtaga atttaagagt ttaatggatt atgcaatgaa gtttaaattt gatgattggt
     7021 tcccgctttt atgtgataac tcggcgtatg ttattcaaga atatataaaa tcaaatcggt
     7081 ataaatctga ccgatagatt ttgaatttag gtgtctgcag ttgaaatccc ctcaaaaacc
     7141 cgatataatg ggtttaatag ctgaataaga acggtgctct ccaaatattc ttatttagaa
     7201 aagcaaatct aaaattatct gaaaggggaa tgagaatagt gaatggacca ataataatga
     7261 ctagagaaga aagaatgaag attgttcatg aaattaagga acgaatattg gataaatatg
     7321 gggatgatgt taaggctatt ggtgtttatg gctctcttgg tcgtcagact gatgggccct
     7381 attcggatat tgagatgatg tgtgtcatgt caacagagga agcagagttc agccatgaat
     7441 ggacaaccgg tgagtggaag gtggaagtga attttgatag cgaagagatt ctactagatt
     7501 atgcatctca ggtggaatca gattggccgc ttacacatgg tcaatttttc tctattttgc
     7561 cgatttatga ttcaggtgga tacttagaga aagtgtatca aactgctaaa tcggtagaag
     7621 cccaaacgtt ccacgatgcg atttgtgccc ttatcgtaga agagctgttt gaatatgcag
     7681 gcaaatggcg taatattcgt gtgcaaggac cgacaacatt tctaccatcc ttgactgtgc
     7741 aggtagcaat ggcaggtgcc atgttgattg gtctgcatca tcgcatctgt tatacgacga
     7801 gcgcttcggt cttaactgaa gcagttaagc aatcagatct tccttcaggt tatgaccatc
     7861 tgtgccagtt cgtaatgtct ggtcaacttt ccgactctga gaaacttctg gaatcgctag
     7921 agaatttctg gaatgggatt caggagtgga cagaacgaca cggatatata gtggatgtgt
     7981 caaaacgcat accattttga acgatgacct cgagctcggc tgacttcagg tgctacattt
     8041 gaagagataa attgcactga aatctagaaa tattttatct gattaataag atgatcttc
//
