LOCUS       sequence-327906-       10678 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  tag TRF1 with GFP and Halo.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10678)
  AUTHORS   Zhang H, Zhao R, Tones J, Liu M, Dilley RL, Chenoweth DM, Greenberg 
            RA, Lampson MA
  TITLE     Nuclear body phase separation drives telomere clustering in ALT 
            cancer cells.
  JOURNAL   Mol Biol Cell. 2020 Aug 15;31(18):2048-2056. doi: 
            10.1091/mbc.E19-10-0589. Epub 2020 Jun 24.
  PUBMED    32579423
REFERENCE   2  (bases 1 to 10678)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1091/mbc.E19-10-0589"; journalName: "Mol Biol Cell"; date: 
            "2020-08-15- 15"; volume: "31"; issue: "18"; pages: "2048-2056"
COMMENT     Sequence Label: 3XHalo_GFP_TRF1#1
FEATURES             Location/Qualifiers
     source          1..10678
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(92..883,884..952))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     715..734
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(953..1057)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    1130..1178
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     protein_bind    1390..1423
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     CDS             1428..2027
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(1428..1447)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     1924..1944
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     complement(2163..2180)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    2169..2280
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     enhancer        2425..2728
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2730..3007
                     /label=chicken beta-actin promoter
     intron          3008..4024
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     3948..3970
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     4032..4051
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             4100..4990
                     /codon_start=1
                     /product="modified bacterial dehalogenase that forms 
                     covalent bonds with chloroalkane derivatives"
                     /label=HaloTag(R)
                     /translation="MAEIGTGFPFDPHYVEVLGERMHYVDVGPRDGTPVLFLHGNPTSS
                     YVWRNIIPHVAPTHRCIAPDLIGMGKSDKPDLGYFFDDHVRFMDAFIEALGLEEVVLVI
                     HDWGSALGFHWAKRNPERVKGIAFMEFIRPIPTWDEWPEFARETFQAFRTTDVGRKLII
                     DQNVFIEGTLPMGVVRPLTEVEMDHYREPFLNPVDREPLWRFPNELPIAGEPANIVALV
                     EEYMDWLHQSPVPKLLFWGTPGVLIPPAEAARLAKSLPNCKAVDIGPGLNLLQEDNPDL
                     IGSEIARWLSTLEISG"
     CDS             5018..5905
                     /codon_start=1
                     /product="modified bacterial dehalogenase that forms 
                     covalent bonds with chloroalkane derivatives"
                     /label=HaloTag(R)
                     /translation="AEIGTGFPFDPHYVEVLGERMHYVDVGPRDGTPVLFLHGNPTSSY
                     VWRNIIPHVAPTHRCIAPDLIGMGKSDKPDLGYFFDDHVRFMDAFIEALGLEEVVLVIH
                     DWGSALGFHWAKRNPERVKGIAFMEFIRPIPTWDEWPEFARETFQAFRTTDVGRKLIID
                     QNVFIEGTLPMGVVRPLTEVEMDHYREPFLNPVDREPLWRFPNELPIAGEPANIVALVE
                     EYMDWLHQSPVPKLLFWGTPGVLIPPAEAARLAKSLPNCKAVDIGPGLNLLQEDNPDLI
                     GSEIARWLSTLEISG"
     regulatory      5936..5945
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5942..6832
                     /codon_start=1
                     /product="modified bacterial dehalogenase that forms 
                     covalent bonds with chloroalkane derivatives"
                     /label=HaloTag(R)
                     /translation="MAEIGTGFPFDPHYVEVLGERMHYVDVGPRDGTPVLFLHGNPTSS
                     YVWRNIIPHVAPTHRCIAPDLIGMGKSDKPDLGYFFDDHVRFMDAFIEALGLEEVVLVI
                     HDWGSALGFHWAKRNPERVKGIAFMEFIRPIPTWDEWPEFARETFQAFRTTDVGRKLII
                     DQNVFIEGTLPMGVVRPLTEVEMDHYREPFLNPVDREPLWRFPNELPIAGEPANIVALV
                     EEYMDWLHQSPVPKLLFWGTPGVLIPPAEAARLAKSLPNCKAVDIGPGLNLLQEDNPDL
                     IGSEIARWLSTLEISG"
     regulatory      6839..6848
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(6845..6847,6848..6850,6851..7561)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(6845..6847,6848..6850,6851..7561)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(6890..6911)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(7151..7170)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     7498..7519
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(8900..8919)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    8965..9020
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(9019..9038)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     protein_bind    9384..9417
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     polyA_signal    9430..9551
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9467..9486)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9521..9540
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(9570..9590)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(9570..9588)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(9609..9625)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9609..9625)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9622..9644)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9633..9649
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9657..9663,9664..9681,9682..9687))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9702..9723
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9840..9857)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10011..10599)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10091..10110)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct
       61 aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta
      121 tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa
      181 ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac
      241 gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa
      301 gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag
      361 taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg
      421 tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag
      481 ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg
      541 tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc
      601 ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat
      661 tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata
      721 ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa
      781 aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca
      841 actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc
      901 aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc
      961 tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg
     1021 aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccaa
     1081 gcttaataaa cacggaagga gacaataccg gaaggaaccc gcgctatgac ggcaataaaa
     1141 agacagaata aaacgcacgg gtgttgggtc gtttgttcat aaacgcgggg ttcggtccca
     1201 gggctggcac tctgtcgata ccccaccgag accccattgg ggccaatacg cccgcgtttc
     1261 ttccttttcc ccaccccacc ccccaagttc gggtgaaggc ccagggctcg cagccaacgt
     1321 cggggcggca ggccctgcca tagccactgg ccccgtgggt tagggacggg gtcccccatt
     1381 cgagaataaa taacttcgta taaagtatcc tatacgaagt tatcaccatg accgagtaca
     1441 agcccacggt gcgcctcgcc acccgcgacg acgtcccccg ggccgtacgc accctcgccg
     1501 ccgcgttcgc cgactacccc gccacgcgcc acaccgtcga cccggaccgc cacatcgagc
     1561 gggtcaccga gctgcaagaa ctcttcctca cgcgcgtcgg gctcgacatc ggcaaggtgt
     1621 gggtcgcgga cgacggcgcc gcggtggcgg tctggaccac gccggagagc gtcgaagcgg
     1681 gggcggtgtt cgccgagatc ggcccgcgca tggccgagtt gagcggttcc cggctggccg
     1741 cgcagcaaca gatggaaggc ctcctggcgc cgcaccggcc caaggagccc gcgtggttcc
     1801 tggccaccgt cggcgtctcg cccgaccacc agggcaaggg tctgggcagc gccgtcgtgc
     1861 tccccggagt ggaggcggcc gagcgcgccg gggtgcccgc cttcctggag acctccgcgc
     1921 cccgcaacct ccccttctac gagcggctcg gcttcaccgt caccgccgac gtcgaggtgc
     1981 ccgaaggacc gcgcacctgg tgcatgaccc gcaagcccgg tgcctgacgc ccgccccacg
     2041 acccgcagcg cccgaccgaa aggagcgcac gaccccatgg ctccgaccga agccacccgg
     2101 ggcggccccg ccgaccccgc acccgccccc gaggcccacc gactctagag ctcgctgatc
     2161 agcctcgact gtgccttcta gttgccagcc atctgttgtt tgcccctccc ccgtgccttc
     2221 cttgaccctg gaaggtgcca ctcccactgt cctttcctaa taaaatgagg aaattgcatc
     2281 gcattgtctg agtaggtgtc attctattct ggggggtggg gtggggcagg acagcaaggg
     2341 ggaggattgg gaagacaata gcactagtta ttaatagtaa tcaattacgg ggtcattagt
     2401 tcatagccca tatatggagt tccgcgttac ataacttacg gtaaatggcc cgcctggctg
     2461 accgcccaac gacccccgcc cattgacgtc aataatgacg tatgttccca tagtaacgcc
     2521 aatagggact ttccattgac gtcaatgggt ggagtattta cggtaaactg cccacttggc
     2581 agtacatcaa gtgtatcata tgccaagtac gccccctatt gacgtcaatg acggtaaatg
     2641 gcccgcctgg cattatgccc agtacatgac cttatgggac tttcctactt ggcagtacat
     2701 ctacgtatta gtcatcgcta ttaccatggt cgaggtgagc cccacgttct gcttcactct
     2761 ccccatctcc cccccctccc cacccccaat tttgtattta tttatttttt aattattttg
     2821 tgcagcgatg ggggcggggg gggggggggg gcgcgcgcca ggcggggcgg ggcggggcga
     2881 ggggcggggc ggggcgaggc ggagaggtgc ggcggcagcc aatcagagcg gcgcgctccg
     2941 aaagtttcct tttatggcga ggcggcggcg gcggcggccc tataaaaagc gaagcgcgcg
     3001 gcgggcggga gtcgctgcgc gctgccttcg ccccgtgccc cgctccgccg ccgcctcgcg
     3061 ccgcccgccc cggctctgac tgaccgcgtt actcccacag gtgagcgggc gggacggccc
     3121 ttctcctccg ggctgtaatt agcgcttggt ttaatgacgg cttgtttctt ttctgtggct
     3181 gcgtgaaagc cttgaggggc tccgggaggg ccctttgtgc ggggggagcg gctcgggggg
     3241 tgcgtgcgtg tgtgtgtgcg tggggagcgc cgcgtgcggc tccgcgctgc ccggcggctg
     3301 tgagcgctgc gggcgcggcg cggggctttg tgcgctccgc agtgtgcgcg aggggagcgc
     3361 ggccgggggc ggtgccccgc ggtgcggggg gggctgcgag gggaacaaag gctgcgtgcg
     3421 gggtgtgtgc gtgggggggt gagcaggggg tgtgggcgcg tcggtcgggc tgcaaccccc
     3481 cctgcacccc cctccccgag ttgctgagca cggcccggct tcgggtgcgg ggctccgtac
     3541 ggggcgtggc gcggggctcg ccgtgccggg cggggggtgg cggcaggtgg gggtgccggg
     3601 cggggcgggg ccgcctcggg ccggggaggg ctcgggggag gggcgcggcg gcccccggag
     3661 cgccggcggc tgtcgaggcg cggcgagccg cagccattgc cttttatggt aatcgtgcga
     3721 gagggcgcag ggacttcctt tgtcccaaat ctgtgcggag ccgaaatctg ggaggcgccg
     3781 ccgcaccccc tctagcgggc gcggggcgaa gcggtgcggc gccggcagga aggaaatggg
     3841 cggggagggc cttcgtgcgt cgccgcgccg ccgtcccctt ctccctctcc agcctcgggg
     3901 ctgtccgcgg ggggacggct gccttcgggg gggacggggc agggcggggt tcggcttctg
     3961 gcgtgtgacc ggcggctcta gagcctctgc taaccatgtt catgccttct tctttttcct
     4021 acagctcctg ggcaacgtgc tggttattgt gctgtctcat cattttggca aagaattgct
     4081 cgaccaccgg cgcgccacaa tggcagagat cggtactggc ttcccattcg atccccatta
     4141 cgtggaagtc ctgggcgaac gcatgcacta tgtcgatgtt ggtccgcgcg acggcacccc
     4201 tgtgctgttc ctgcatggta acccgacctc ctcctatgtg tggcgcaaca ttatcccgca
     4261 cgttgcaccg acccatcgct gtattgctcc agatctgatc ggtatgggca agtccgacaa
     4321 gccagacctg ggttacttct ttgacgatca cgtccgcttt atggatgcct ttatcgaggc
     4381 cctgggtctg gaagaagtcg tcctggtcat tcatgactgg ggctccgctc tgggttttca
     4441 ctgggccaaa cgcaatccag aacgcgtcaa aggtatcgca tttatggaat tcattcgccc
     4501 tatcccgacc tgggacgaat ggccagagtt tgcccgcgaa accttccagg cctttcgcac
     4561 caccgacgtc ggccgcaaac tgatcattga tcagaatgtt tttattgagg gtacgctgcc
     4621 gatgggtgtc gtccgcccgc tgactgaggt cgagatggat cattatcgcg agccgtttct
     4681 gaatcctgtt gatcgcgagc cactgtggcg ctttccaaac gaactgccaa tcgccggtga
     4741 accagcgaac attgtcgcgc tggtcgaaga gtacatggat tggctgcacc agtcccctgt
     4801 cccgaaactg ctgttctggg gcaccccagg cgttctgatt ccaccggccg aagccgctcg
     4861 cctggccaag agcctgccta actgcaaagc tgtggacatt ggcccgggtc tgaatctgct
     4921 gcaagaggac aatccggacc tgattggcag cgagattgcg cgctggctgt cgacgctcga
     4981 gatctccggc gcttcgaatt ctgcagtcga cggtaccgca gaaattggta ctggctttcc
     5041 atttgacccc cactatgtgg aggtcctggg cgagcgcatg cattacgtcg acgttggtcc
     5101 gcgcgatggc acccctgtgc tgtttctgca cggtaatccg acctcctcct acgtgtggcg
     5161 caatatcatt ccgcatgttg caccgaccca ccgctgcatc gctccagacc tgattggtat
     5221 gggcaaatcc gataaaccag atctgggtta ttttttcgat gaccatgtcc gcttcatgga
     5281 cgccttcatt gaagccctgg gtctggagga ggtcgtcctg gtcatccacg attggggctc
     5341 cgctctgggt ttccattggg ccaagcgcaa cccagagcgc gtcaagggta ttgcattcat
     5401 ggagtttatc cgccctattc cgacctggga cgagtggcca gaattcgccc gcgagacctt
     5461 tcaggccttc cgcaccaccg atgtcggccg caagctgatt atcgaccaga acgttttcat
     5521 cgaaggtacg ctgccgatgg gtgtcgtccg cccgctgact gaagtcgaaa tggaccacta
     5581 ccgcgaaccg ttcctgaacc ctgttgaccg cgaaccactg tggcgcttcc caaatgagct
     5641 gccaattgcc ggtgagccag cgaatatcgt cgcgctggtc gaggaatata tggactggct
     5701 gcatcagtcc cctgtcccga agctgctgtt ttggggcacc ccaggcgttc tgatcccacc
     5761 ggccgaggcc gctcgcctgg ccaaaagcct gcctaattgc aaggctgtgg atatcggccc
     5821 gggtctgaac ctgctgcaag aagataaccc ggatctgatc ggcagcgaaa tcgcgcgctg
     5881 gctgtcgacg ctcgaaattt ccggcagtgc tggtagtgct ggtgaattga tgcgcgccac
     5941 catggcagaa atcggtactg gctttccatt cgacccccat tatgtggaag tcctgggcga
     6001 gcgcatgcac tacgtcgatg ttggtccgcg cgatggcacc cctgtgctgt tcctgcacgg
     6061 taacccgacc tcctcctacg tgtggcgcaa catcatcccg catgttgcac cgacccatcg
     6121 ctgcattgct ccagacctga tcggtatggg caaatccgac aaaccagacc tgggttattt
     6181 cttcgacgac cacgtccgct tcatggatgc cttcatcgaa gccctgggtc tggaagaggt
     6241 cgtcctggtc attcacgact ggggctccgc tctgggtttc cactgggcca agcgcaatcc
     6301 agagcgcgtc aaaggtattg catttatgga gttcatccgc cctatcccga cctgggacga
     6361 atggccagaa tttgcccgcg agaccttcca ggccttccgc accaccgacg tcggccgcaa
     6421 gctgatcatc gatcagaacg tttttatcga gggtacgctg ccgatgggtg tcgtccgccc
     6481 gctgactgaa gtcgagatgg accattaccg cgagccgttc ctgaatcctg ttgaccgcga
     6541 gccactgtgg cgcttcccaa acgagctgcc aatcgccggt gagccagcga acatcgtcgc
     6601 gctggtcgaa gaatacatgg actggctgca ccagtcccct gtcccgaagc tgctgttctg
     6661 gggcacccca ggcgttctga tcccaccggc cgaagccgct cgcctggcca aaagcctgcc
     6721 taactgcaag gctgtggaca tcggcccggg tctgaatctg ctgcaagaag acaacccgga
     6781 cctgatcggc agcgagatcg cgcgctggct gtcgacgctc gagatttccg gttacgtagc
     6841 caccatggtg agcaagggcg aggagctgtt caccggggtg gtgcccatcc tggtcgagct
     6901 ggacggcgac gtaaacggcc acaagttcag cgtgtccggc gagggcgagg gcgatgccac
     6961 ctacggcaag ctgaccctga agttcatctg caccaccggc aagctgcccg tgccctggcc
     7021 caccctcgtg accaccctga cctacggcgt gcagtgcttc agccgctacc ccgaccacat
     7081 gaagcagcac gacttcttca agtccgccat gcccgaaggc tacgtccagg agcgcaccat
     7141 cttcttcaag gacgacggca actacaagac ccgcgccgag gtgaagttcg agggcgacac
     7201 cctggtgaac cgcatcgagc tgaagggcat cgacttcaag gaggacggca acatcctggg
     7261 gcacaagctg gagtacaact acaacagcca caacgtctat atcatggccg acaagcagaa
     7321 gaacggcatc aaggtgaact tcaagatccg ccacaacatc gaggacggca gcgtgcagct
     7381 cgccgaccac taccagcaga acacccccat cggcgacggc cccgtgctgc tgcccgacaa
     7441 ccactacctg agcacccagt ccgccctgag caaagacccc aacgagaagc gcgatcacat
     7501 ggtcctgctg gagttcgtga ccgccgccgg gatcactctc ggcatggacg agctgtacaa
     7561 gagggatgcc gaccctactg aggagcagat ggcagaaaca gagagaaacg acgaggagca
     7621 gttcgaatgc caggaactgc tcgagtgcca ggtgcaggtg ggggcccccg aggaggagga
     7681 ggaggaggag gaggacgcgg gcctggtggc cgaggccgag gccgtggctg ccggctggat
     7741 gctcgatttc ctctgcctct ctctttgccg agctttccgc gacggccgct ccgaggactt
     7801 ccgcaggacc cgcaacagcg cagaggctat tattcatgga ctatccagtc taacagcttg
     7861 ccagttgaga acgatataca tatgtcagtt tttgacaaga attgcagcag gaaaaaccct
     7921 tgatgcacag tttgaaaatg atgaacgaat tacacccttg gaatcagccc tgatgatttg
     7981 gggttcaatt gaaaaggaac atgacaaact tcatgaagaa atacagaatt taattaaaat
     8041 tcaggctata gctgtttgta tggaaaatgg caactttaaa gaagcagaag aagtctttga
     8101 aagaatattt ggtgatccaa attctcatat gcctttcaaa agcaaattgc ttatgataat
     8161 ctctcagaaa gatacatttc attccttttt tcaacacttc agctacaacc acatgatgga
     8221 gaaaattaag agttatgtga attatgtgct aagtgaaaaa tcatcaacct ttctaatgaa
     8281 ggcagcggca aaagtagtag aaagcaaaag gacaagaaca ataacttctc aagataaacc
     8341 tagtggtaat gatgttgaaa tggaaactga agctaatttg gatacaagaa aaagtgttag
     8401 tgacaaacag tctgcggtaa ctgaatcctc agagggtaca gtatccttat tgaggtctca
     8461 caagaatctt ttcttatcta agttgcaaca tggaacccag caacaagacc ttaataagaa
     8521 agaaagaaga gtaggaactc ctcaaagtac aaaaaagaaa aaagaaagca gaagagccac
     8581 tgaaagcaga atacctgttt caaagagtca gccggtaact cctgaaaaac atcgagctag
     8641 aaaaagacag gcatggcttt gggaagaaga caagaatttg agatctggcg tgaggaaata
     8701 tggagaggga aactggtcta aaatactgtt gcattataaa ttcaacaacc ggacaagtgt
     8761 catgttaaaa gacagatgga ggaccatgaa gaaactaaaa ctgatttcct cagacagcga
     8821 agactaatca ggtgcaggct gcctatcaga aggtggtggc tggtgtggcc aatgccctgg
     8881 ctcacaaata ccactgagat ctttttccct ctgccaaaaa ttatggggac atcatgaagc
     8941 cccttgagca tctgacttct ggctaataaa ggaaatttat tttcattgca atagtgtgtt
     9001 ggaatttttt gtgtctctca ctcggaagga catatgggag ggcaaatcat ttaaaacatc
     9061 agaatgagta tttggtttag agtttggcaa catatgccca tatgctggct gccatgaaca
     9121 aaggttggct ataaagaggt catcagtata tgaaacagcc ccctgctgtc cattccttat
     9181 tccatagaaa agccttgact tgaggttaga ttttttttat attttgtttt gtgttatttt
     9241 tttctttaac atccctaaaa ttttccttac atgttttact agccagattt ttcctcctct
     9301 cctgactact cccagtcata gctgtccctc ttctcttatg gagatccctc gacctgcacc
     9361 gtcgagctag catatggatc catataactt cgtataatgt atgctatacg aagttattac
     9421 gataccgtca acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca
     9481 aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc
     9541 aatgtatctt aggtacccag cttttgttcc ctttagtgag ggttaattgc gcgcttggcg
     9601 taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac
     9661 atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca
     9721 ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat
     9781 taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc
     9841 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca
     9901 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca
     9961 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
    10021 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
    10081 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
    10141 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
    10201 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
    10261 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
    10321 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
    10381 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
    10441 tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
    10501 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
    10561 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
    10621 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagat
//
