LOCUS       sequence-327908-        8671 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of candidate TCR #1 TCRalpha cDNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8671)
  AUTHORS   Lee MN, Meyerson M
  TITLE     Antigen identification for HLA class I- and HLA class II-restricted 
            T cell receptors using cytokine-capturing antigen-presenting cells.
  JOURNAL   Sci Immunol. 2021 Jan 22;6(55). pii: 6/55/eabf4001. doi: 
            10.1126/sciimmunol.abf4001.
  PUBMED    33483338
REFERENCE   2  (bases 1 to 8671)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci 
            Immunol."; date: "2021-01-22"; volume: "6(55). pii: 6/55/eabf4001. 
            doi"; pages: " 10.1126/sciimmunol.abf4001"
COMMENT     Sequence Label: CMVTCRa_candidate1
FEATURES             Location/Qualifiers
     source          1..8671
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        65..368
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        369..572
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     523..543
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             complement(883..894)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     misc_feature    1482..2070
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(1535..1555)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(1953..1964)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             2142..2375
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    2453..2574
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2490..2509)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2544..2563
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      2614..2749
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(2769..2788)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2770..2788)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2798..2815)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2798..2814)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2807..2829)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      2956..3411
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3043..3062)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3253..3274
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        3437..3541
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3542..3610,3611..4402)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3760..3779)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4573..5161
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5062..5081
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5315..5332
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    5449..5470
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(5485..5490,5491..5508,5509..5515)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5523..5539
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5528..5550
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     5547..5563
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5547..5563
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     5582..5602
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        5584..5602
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        5630..5856
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             5857..6037
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    6084..6209
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    6702..6935
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             7120..7164
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        7339..7839
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     7758..7776
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             7861..8460
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(7861..7880)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     8357..8377
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    8502..8619
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 cccggggtta ttaatagtaa tcaattacgg ggtcattagt tcatagccca tatatggagt
       61 tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc
      121 cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac
      181 gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata
      241 tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc
      301 agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta
      361 ttaccatggt gatgcggttt tggcagtaca tcaatgggcg tggatagcgg tttgactcac
      421 ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttgg caccaaaatc
      481 aacgggactt tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc
      541 gtgtacggtg ggaggtctat ataagcagag ctctctggct aactgtcggg atcaacaagt
      601 ttggccgcca ccatgctcct ggagcttatc ccactgctgg ggatacattt tgtcctgaga
      661 actgccagag cccagtcagt gacccagcct gacatccaca tcactgtctc tgaaggagcc
      721 tcactggagt tgagatgtaa ctattcctat ggggcaacac cttatctctt ctggtatgtc
      781 cagtcccccg gccaaggcct ccagctgctc ctgaagtact tttcaggaga cactctggtt
      841 caaggcatta aaggctttga ggctgaattt aagaggagtc aatcttcctt caatctgagg
      901 aaaccctctg tgcattggag tgatgctgct gagtacttct gtgctgtggc cagctatgga
      961 aacaaactgg tctttggcgc aggaaccatt ctgagagtca agtcctatat ccagaaccct
     1021 gaccctgccg tgtaccagct gagagactct aaatccagtg acaagtctgt ctgcctattc
     1081 accgattttg attctcaaac aaatgtgtca caaagtaagg attctgatgt gtatatcaca
     1141 gacaaatgtg tgctagacat gaggtctatg gattttaaat ctaattcagc agtagcctgg
     1201 agcaacaaat ctgactttgc atgtgcaaac gccttcaaca acagcattat tccagaagac
     1261 accttcttcc ccagcccaga aagttcctgt gatgtcaagc tggtcgagaa aagctttgaa
     1321 acagatacga acctaaactt tcaaaacctg tcagtgattg ggttccgaat cctcctcctg
     1381 aaagtggccg ggtttaatct gctcatgacg ctgcggctgt ggtccagcta gaaagtggtt
     1441 tagtaatgag ctagcgctaa ccggtggcgc gttaagtcga caatcaacct ctggattaca
     1501 aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg ctatgtggat
     1561 acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct
     1621 ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac
     1681 gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc attgccacca
     1741 cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg gcggaactca
     1801 tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg
     1861 tggtgttgtc ggggaaatca tcgtcctttc cttggctgct cgcctgtgtt gccacctgga
     1921 ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg gaccttcctt
     1981 cccgcggcct gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga
     2041 gtcggatctc cctttgggcc gcctccccgc gtcgacttta agaccaatga cttacaaggc
     2101 agctgtagat cttagccact ttttaaaaga aaagggggga ctggaagggc taattcactc
     2161 ccaacgaaga caagatctgc tttttgcttg tactgggtct ctctggttag accagatctg
     2221 agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc
     2281 ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct
     2341 cagacccttt tagtcagtgt ggaaaatctc tagcagtacg tatagtagtt catgtcatct
     2401 tattattcag tatttataac ttgcaaagaa atgaatatca gagagtgaga ggaacttgtt
     2461 tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc
     2521 atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt
     2581 ctggctctag ctatcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc
     2641 gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc
     2701 tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct agggacgtac
     2761 ccaattcgcc ctatagtgag tcgtattacg cgcgctcact ggccgtcgtt ttacaacgtc
     2821 gtgactggga aaaccctggc gttacccaac ttaatcgcct tgcagcacat ccccctttcg
     2881 ccagctggcg taatagcgaa gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc
     2941 tgaatggcga atgggacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta
     3001 cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc
     3061 cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt
     3121 tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg
     3181 gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca
     3241 cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct
     3301 attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga
     3361 tttaacaaaa atttaacgcg aattttaaca aaatattaac gcttacaatt taggtggcac
     3421 ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat
     3481 gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag
     3541 tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc
     3601 tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc
     3661 acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc
     3721 cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc
     3781 ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt
     3841 ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt
     3901 atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat
     3961 cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct
     4021 tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat
     4081 gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc
     4141 ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg
     4201 ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc
     4261 tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta
     4321 cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc
     4381 ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga
     4441 tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat
     4501 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat
     4561 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
     4621 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
     4681 ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt
     4741 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
     4801 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
     4861 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
     4921 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac
     4981 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
     5041 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
     5101 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
     5161 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat
     5221 gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc
     5281 tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga
     5341 agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg
     5401 gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta
     5461 gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg
     5521 aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt acgccaagcg
     5581 cgcaattaac cctcactaaa gggaacaaaa gctggagctg caagcttaat gtagtcttat
     5641 gcaatactct tgtagtcttg caacatggta acgatgagtt agcaacatgc cttacaagga
     5701 gagaaaaagc accgtgcatg ccgattggtg gaagtaaggt ggtacgatcg tgccttatta
     5761 ggaaggcaac agacgggtct gacatggatt ggacgaacca ctgaattgcc gcattgcaga
     5821 gatattgtat ttaagtgcct agctcgatac ataaacgggt ctctctggtt agaccagatc
     5881 tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg
     5941 ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc
     6001 ctcagaccct tttagtcagt gtggaaaatc tctagcagtg gcgcccgaac agggacttga
     6061 aagcgaaagg gaaaccagag gagctctctc gacgcaggac tcggcttgct gaagcgcgca
     6121 cggcaagagg cgaggggcgg cgactggtga gtacgccaaa aattttgact agcggaggct
     6181 agaaggagag agatgggtgc gagagcgtca gtattaagcg ggggagaatt agatcgcgat
     6241 gggaaaaaat tcggttaagg ccagggggaa agaaaaaata taaattaaaa catatagtat
     6301 gggcaagcag ggagctagaa cgattcgcag ttaatcctgg cctgttagaa acatcagaag
     6361 gctgtagaca aatactggga cagctacaac catcccttca gacaggatca gaagaactta
     6421 gatcattata taatacagta gcaaccctct attgtgtgca tcaaaggata gagataaaag
     6481 acaccaagga agctttagac aagatagagg aagagcaaaa caaaagtaag accaccgcac
     6541 agcaagcggc cgctgatctt cagacctgga ggaggagata tgagggacaa ttggagaagt
     6601 gaattatata aatataaagt agtaaaaatt gaaccattag gagtagcacc caccaaggca
     6661 aagagaagag tggtgcagag agaaaaaaga gcagtgggaa taggagcttt gttccttggg
     6721 ttcttgggag cagcaggaag cactatgggc gcagcgtcaa tgacgctgac ggtacaggcc
     6781 agacaattat tgtctggtat agtgcagcag cagaacaatt tgctgagggc tattgaggcg
     6841 caacagcatc tgttgcaact cacagtctgg ggcatcaagc agctccaggc aagaatcctg
     6901 gctgtggaaa gatacctaaa ggatcaacag ctcctgggga tttggggttg ctctggaaaa
     6961 ctcatttgca ccactgctgt gccttggaat gctagttgga gtaataaatc tctggaacag
     7021 atttggaatc acacgacctg gatggagtgg gacagagaaa ttaacaatta cacaagctta
     7081 atacactcct taattgaaga atcgcaaaac cagcaagaaa agaatgaaca agaattattg
     7141 gaattagata aatgggcaag tttgtggaat tggtttaaca taacaaattg gctgtggtat
     7201 ataaaattat tcataatgat agtaggaggc ttggtaggtt taagaatagt ttttgctgta
     7261 ctttctatag tgaatagagt taggcaggga tattcaccat tatcgtttca gacccacctc
     7321 ccaaccccga ggggaccctt gcgccttttc caaggcagcc ctgggtttgc gcagggacgc
     7381 ggctgctctg ggcgtggttc cgggaaacgc agcggcgccg accctgggtc tcgcacattc
     7441 ttcacgtccg ttcgcagcgt cacccggatc ttcgccgcta cccttgtggg ccccccggcg
     7501 acgcttcctg ctccgcccct aagtcgggaa ggttccttgc ggttcgcggc gtgccggacg
     7561 tgacaaacgg aagccgcacg tctcactagt accctcgcag acggacagcg ccagggagca
     7621 atggcagcgc gccgaccgcg atgggctgtg gccaatagcg gctgctcagc agggcgcgcc
     7681 gagagcagcg gccgggaagg ggcggtgcgg gaggcggggt gtggggcggt agtgtgggcc
     7741 ctgttcctgc ccgcgcggtg ttccgcattc tgcaagcctc cggagcgcac gtcggcagtc
     7801 ggctccctcg ttgaccgaat caccgacctc tctccccagg gggtaccacc ggagcttacc
     7861 atgaccgagt acaagcccac ggtgcgcctc gccacccgcg acgacgtccc cagggccgta
     7921 cgcaccctcg ccgccgcgtt cgccgactac cccgccacgc gccacaccgt cgatccggac
     7981 cgccacatcg agcgggtcac cgagctgcaa gaactcttcc tcacgcgcgt cgggctcgac
     8041 atcggcaagg tgtgggtcgc ggacgacggc gccgccgtgg cggtctggac cacgccggag
     8101 agcgtcgaag cgggggcggt gttcgccgag atcggcccgc gcatggccga gttgagcggt
     8161 tcccggctgg ccgcgcagca acagatggaa ggcctcctgg cgccgcaccg gcccaaggag
     8221 cccgcgtggt tcctggccac cgtcggcgtc tcgcccgacc accagggcaa gggtctgggc
     8281 agcgccgtcg tgctccccgg agtggaggcg gccgagcgcg ccggggtgcc cgccttcctg
     8341 gagacctccg cgccccgcaa cctccccttc tacgagcggc tcggcttcac cgtcaccgcc
     8401 gacgtcgagg tgcccgaagg accgcgcacc tggtgcatga cccgcaagcc cggtgcctga
     8461 gaattgtaga tcttgagaca aatggcagta ttcatccaca attttaaaag aaaagggggg
     8521 attggggggt acagtgcagg ggaaagaata gtagacataa tagcaacaga catacaaact
     8581 aaagaattac aaaaacaaat tacaaaaatt caaaattttc gggtttatta cagggacagc
     8641 agagatccac tttggcgccg gctcgagggg g
//
