LOCUS       sequence-327915-        8681 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of candidate TCR #8 TCRalpha cDNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8681)
  AUTHORS   Lee MN, Meyerson M
  TITLE     Antigen identification for HLA class I- and HLA class II-restricted 
            T cell receptors using cytokine-capturing antigen-presenting cells.
  JOURNAL   Sci Immunol. 2021 Jan 22;6(55). pii: 6/55/eabf4001. doi: 
            10.1126/sciimmunol.abf4001.
  PUBMED    33483338
REFERENCE   2  (bases 1 to 8681)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci 
            Immunol."; date: "2021-01-22"; volume: "6(55). pii: 6/55/eabf4001. 
            doi"; pages: " 10.1126/sciimmunol.abf4001"
COMMENT     Sequence Label: CMVTCRa_candidate8
FEATURES             Location/Qualifiers
     source          1..8681
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        65..368
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        369..572
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     523..543
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      608..617
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    1492..2080
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(1545..1565)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(1963..1974)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             2152..2385
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    2463..2584
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2500..2519)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2554..2573
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      2624..2759
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(2779..2798)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2780..2798)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2808..2825)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2808..2824)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2817..2839)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      2966..3421
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3053..3072)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3263..3284
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        3447..3551
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3552..3620,3621..4412)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3770..3789)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4583..5171
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5072..5091
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5325..5342
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    5459..5480
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(5495..5500,5501..5518,5519..5525)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5533..5549
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5538..5560
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     5557..5573
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5557..5573
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     5592..5612
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        5594..5612
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        5640..5866
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             5867..6047
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    6094..6219
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    6712..6945
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             7130..7174
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        7349..7849
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     7768..7786
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             7871..8470
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(7871..7890)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     8367..8387
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    8512..8629
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 cccggggtta ttaatagtaa tcaattacgg ggtcattagt tcatagccca tatatggagt
       61 tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc
      121 cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac
      181 gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata
      241 tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc
      301 agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta
      361 ttaccatggt gatgcggttt tggcagtaca tcaatgggcg tggatagcgg tttgactcac
      421 ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttgg caccaaaatc
      481 aacgggactt tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc
      541 gtgtacggtg ggaggtctat ataagcagag ctctctggct aactgtcggg atcaacaagt
      601 ttgtgccgcc accatggcct ctgcacccat ctcgatgctt gcgatgctct tcacattgag
      661 tgggctgaga gctcagtcag tggctcagcc ggaagatcag gtcaacgttg ctgaagggaa
      721 tcctctgact gtgaaatgca cctattcagt ctctggaaac ccttatcttt tttggtatgt
      781 tcaatacccc aaccgaggcc tccagttcct tctgaaatac atcacagggg ataacctggt
      841 taaaggcagc tatggctttg aagctgaatt taacaagagc caaacctcct tccacctgaa
      901 gaaaccatct gcccttgtga gcgactccgc tttgtacttc tgtgctgtga ccgacgcatc
      961 aggaggaagc tacataccta catttggaag aggaaccagc cttattgttc atccgtatat
     1021 ccagaaccct gaccctgccg tgtaccagct gagagactct aaatccagtg acaagtctgt
     1081 ctgcctattc accgattttg attctcaaac aaatgtgtca caaagtaagg attctgatgt
     1141 gtatatcaca gacaaatgtg tgctagacat gaggtctatg gattttaaat ctaattcagc
     1201 agtagcctgg agcaacaaat ctgactttgc atgtgcaaac gccttcaaca acagcattat
     1261 tccagaagac accttcttcc ccagcccaga aagttcctgt gatgtcaagc tggtcgagaa
     1321 aagctttgaa acagatacga acctaaactt tcaaaacctg tcagtgattg ggttccgaat
     1381 cctcctcctg aaagtggccg ggtttaatct gctcatgacg ctgcggctgt ggtccagcta
     1441 gaaagtggtt tagtaatgag ctagcgctaa ccggtggcgc gttaagtcga caatcaacct
     1501 ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg
     1561 ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc
     1621 attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt
     1681 gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc
     1741 attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg
     1801 gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact
     1861 gacaattccg tggtgttgtc ggggaaatca tcgtcctttc cttggctgct cgcctgtgtt
     1921 gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg
     1981 gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc
     2041 cctcagacga gtcggatctc cctttgggcc gcctccccgc gtcgacttta agaccaatga
     2101 cttacaaggc agctgtagat cttagccact ttttaaaaga aaagggggga ctggaagggc
     2161 taattcactc ccaacgaaga caagatctgc tttttgcttg tactgggtct ctctggttag
     2221 accagatctg agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat
     2281 aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact
     2341 agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtacg tatagtagtt
     2401 catgtcatct tattattcag tatttataac ttgcaaagaa atgaatatca gagagtgaga
     2461 ggaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca
     2521 caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat
     2581 cttatcatgt ctggctctag ctatcccgcc cctaactccg cccatcccgc ccctaactcc
     2641 gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc
     2701 cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct
     2761 agggacgtac ccaattcgcc ctatagtgag tcgtattacg cgcgctcact ggccgtcgtt
     2821 ttacaacgtc gtgactggga aaaccctggc gttacccaac ttaatcgcct tgcagcacat
     2881 ccccctttcg ccagctggcg taatagcgaa gaggcccgca ccgatcgccc ttcccaacag
     2941 ttgcgcagcc tgaatggcga atgggacgcg ccctgtagcg gcgcattaag cgcggcgggt
     3001 gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc
     3061 gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg
     3121 gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat
     3181 tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg
     3241 ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct
     3301 atctcggtct attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa
     3361 aatgagctga tttaacaaaa atttaacgcg aattttaaca aaatattaac gcttacaatt
     3421 taggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
     3481 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
     3541 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
     3601 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
     3661 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
     3721 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
     3781 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc
     3841 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
     3901 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
     3961 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
     4021 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
     4081 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
     4141 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
     4201 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
     4261 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
     4321 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
     4381 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
     4441 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
     4501 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
     4561 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     4621 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     4681 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt
     4741 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     4801 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     4861 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     4921 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
     4981 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     5041 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     5101 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
     5161 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
     5221 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
     5281 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
     5341 aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt
     5401 aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta
     5461 atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta
     5521 tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt
     5581 acgccaagcg cgcaattaac cctcactaaa gggaacaaaa gctggagctg caagcttaat
     5641 gtagtcttat gcaatactct tgtagtcttg caacatggta acgatgagtt agcaacatgc
     5701 cttacaagga gagaaaaagc accgtgcatg ccgattggtg gaagtaaggt ggtacgatcg
     5761 tgccttatta ggaaggcaac agacgggtct gacatggatt ggacgaacca ctgaattgcc
     5821 gcattgcaga gatattgtat ttaagtgcct agctcgatac ataaacgggt ctctctggtt
     5881 agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca
     5941 ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa
     6001 ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagtg gcgcccgaac
     6061 agggacttga aagcgaaagg gaaaccagag gagctctctc gacgcaggac tcggcttgct
     6121 gaagcgcgca cggcaagagg cgaggggcgg cgactggtga gtacgccaaa aattttgact
     6181 agcggaggct agaaggagag agatgggtgc gagagcgtca gtattaagcg ggggagaatt
     6241 agatcgcgat gggaaaaaat tcggttaagg ccagggggaa agaaaaaata taaattaaaa
     6301 catatagtat gggcaagcag ggagctagaa cgattcgcag ttaatcctgg cctgttagaa
     6361 acatcagaag gctgtagaca aatactggga cagctacaac catcccttca gacaggatca
     6421 gaagaactta gatcattata taatacagta gcaaccctct attgtgtgca tcaaaggata
     6481 gagataaaag acaccaagga agctttagac aagatagagg aagagcaaaa caaaagtaag
     6541 accaccgcac agcaagcggc cgctgatctt cagacctgga ggaggagata tgagggacaa
     6601 ttggagaagt gaattatata aatataaagt agtaaaaatt gaaccattag gagtagcacc
     6661 caccaaggca aagagaagag tggtgcagag agaaaaaaga gcagtgggaa taggagcttt
     6721 gttccttggg ttcttgggag cagcaggaag cactatgggc gcagcgtcaa tgacgctgac
     6781 ggtacaggcc agacaattat tgtctggtat agtgcagcag cagaacaatt tgctgagggc
     6841 tattgaggcg caacagcatc tgttgcaact cacagtctgg ggcatcaagc agctccaggc
     6901 aagaatcctg gctgtggaaa gatacctaaa ggatcaacag ctcctgggga tttggggttg
     6961 ctctggaaaa ctcatttgca ccactgctgt gccttggaat gctagttgga gtaataaatc
     7021 tctggaacag atttggaatc acacgacctg gatggagtgg gacagagaaa ttaacaatta
     7081 cacaagctta atacactcct taattgaaga atcgcaaaac cagcaagaaa agaatgaaca
     7141 agaattattg gaattagata aatgggcaag tttgtggaat tggtttaaca taacaaattg
     7201 gctgtggtat ataaaattat tcataatgat agtaggaggc ttggtaggtt taagaatagt
     7261 ttttgctgta ctttctatag tgaatagagt taggcaggga tattcaccat tatcgtttca
     7321 gacccacctc ccaaccccga ggggaccctt gcgccttttc caaggcagcc ctgggtttgc
     7381 gcagggacgc ggctgctctg ggcgtggttc cgggaaacgc agcggcgccg accctgggtc
     7441 tcgcacattc ttcacgtccg ttcgcagcgt cacccggatc ttcgccgcta cccttgtggg
     7501 ccccccggcg acgcttcctg ctccgcccct aagtcgggaa ggttccttgc ggttcgcggc
     7561 gtgccggacg tgacaaacgg aagccgcacg tctcactagt accctcgcag acggacagcg
     7621 ccagggagca atggcagcgc gccgaccgcg atgggctgtg gccaatagcg gctgctcagc
     7681 agggcgcgcc gagagcagcg gccgggaagg ggcggtgcgg gaggcggggt gtggggcggt
     7741 agtgtgggcc ctgttcctgc ccgcgcggtg ttccgcattc tgcaagcctc cggagcgcac
     7801 gtcggcagtc ggctccctcg ttgaccgaat caccgacctc tctccccagg gggtaccacc
     7861 ggagcttacc atgaccgagt acaagcccac ggtgcgcctc gccacccgcg acgacgtccc
     7921 cagggccgta cgcaccctcg ccgccgcgtt cgccgactac cccgccacgc gccacaccgt
     7981 cgatccggac cgccacatcg agcgggtcac cgagctgcaa gaactcttcc tcacgcgcgt
     8041 cgggctcgac atcggcaagg tgtgggtcgc ggacgacggc gccgccgtgg cggtctggac
     8101 cacgccggag agcgtcgaag cgggggcggt gttcgccgag atcggcccgc gcatggccga
     8161 gttgagcggt tcccggctgg ccgcgcagca acagatggaa ggcctcctgg cgccgcaccg
     8221 gcccaaggag cccgcgtggt tcctggccac cgtcggcgtc tcgcccgacc accagggcaa
     8281 gggtctgggc agcgccgtcg tgctccccgg agtggaggcg gccgagcgcg ccggggtgcc
     8341 cgccttcctg gagacctccg cgccccgcaa cctccccttc tacgagcggc tcggcttcac
     8401 cgtcaccgcc gacgtcgagg tgcccgaagg accgcgcacc tggtgcatga cccgcaagcc
     8461 cggtgcctga gaattctaga tcttgagaca aatggcagta ttcatccaca attttaaaag
     8521 aaaagggggg attggggggt acagtgcagg ggaaagaata gtagacataa tagcaacaga
     8581 catacaaact aaagaattac aaaaacaaat tacaaaaatt caaaattttc gggtttatta
     8641 cagggacagc agagatccac tttggcgccg gctcgagggg g
//
