LOCUS       sequence-327922-        9331 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of FLAG-tagged TAPBPR with tapasin CT.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 9331)
  AUTHORS   McShan AC, Devlin CA, Morozov GI, Overall SA, Moschidi D, Akella N, 
            Procko E, Sgourakis NG
  TITLE     TAPBPR promotes antigen loading on MHC-I molecules using a peptide 
            trap.
  JOURNAL   Nat Commun. 2021 May 26;12(1):3174. doi: 10.1038/s41467-021-23225-6.
  PUBMED    34039964
REFERENCE   2  (bases 1 to 9331)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2021-05-26"; volume: "12(1):3174. doi"; pages: " 
            10.1038/s41467-021-23225-6"
COMMENT     Sequence Label: pCEP4-FLAG-TAPBPR-tapasinCT
FEATURES             Location/Qualifiers
     source          1..9331
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(1166..1184)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1252..1356
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1357..1425,1426..2217)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1575..1594)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2388..2976
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2877..2896
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        3407..3552
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     CDS             3595..4632
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPDRE
                     MGEAN"
     primer_bind     complement(4630..4649)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    4674..4722
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     enhancer        5376..5755
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        5756..5959
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     5910..5930
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     5956..5980
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      6008..6017
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             6092..6115
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     polyA_signal    7599..7733
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7623..7642)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     7677..7696
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(7876..7898)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
ORIGIN
        1 gattgtaacc ccgctaacca ctgcggtcaa accacttgcc cacaaaacca ctaatggcac
       61 cccggggaat acctgcataa gtaggtgggc gggccaagat aggggcgcga ttgctgcgat
      121 ctggaggaca aattacacac acttgcgcct gagcgccaag cacagggttg ttggtcctca
      181 tattcacgag gtcgctgaga gcacggtggg ctaatgttgc catgggtagc atatactacc
      241 caaatatctg gatagcatat gctatcctaa tctatatctg ggtagcatag gctatcctaa
      301 tctatatctg ggtagcatat gctatcctaa tctatatctg ggtagtatat gctatcctaa
      361 tttatatctg ggtagcatag gctatcctaa tctatatctg ctcctgcccc tcctcctgct
      421 cctgcccctc ctgctcctgc ccctcccgct cctgctcctg ctcctgttcc accgtgggtc
      481 cctttgcagc caatgcaact tggacgtttt tggggtctcc ggacaccatc tctatgtctt
      541 ggccctgatc ctgagccgcc cggggctcct ggtcttccgc ctcctcgtcc tcgtcctctt
      601 ccccgtcctc gtccatggtt atcaccccct cttctttgag gtccactgcc gccggagcct
      661 tctggtccag atgtgtctcc cttctctcct aggccatttc caggtcctgt acctggcccc
      721 tcgtcagaca tgattcacac taaaagagat caatagacat ctttattaga cgacgctcag
      781 tgaatacagg gagtgcagac tcctgccccc tccaacagcc cccccaccct catccccttc
      841 atggtcgctg tcagacagat ccaggtctga aaattcccca tcctccgaac catcctcgtc
      901 ctcatcacca attactcgca gcccggaaaa ctcccgctga acatcctcaa gatttgcgtc
      961 ctgagcctca agccaggcct caaattcctc gtcccccttt ttgctggacg gtagggatgg
     1021 ggattctcgg gacccctcct cttcctcttc aaggtcacca gacagagatg ctactggggc
     1081 aacggaagaa aagctgggtg cggcctgtga ggatcagctt atcgatgata agctgtcaaa
     1141 catgagaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc
     1201 atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc
     1261 cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc
     1321 tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc
     1381 gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg
     1441 gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat
     1501 ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc
     1561 acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa
     1621 ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa
     1681 aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt
     1741 gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct
     1801 tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat
     1861 gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg
     1921 cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg
     1981 atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt
     2041 attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg
     2101 ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg
     2161 gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg
     2221 tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa
     2281 aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt
     2341 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt
     2401 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt
     2461 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag
     2521 ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta
     2581 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat
     2641 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg
     2701 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg
     2761 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac
     2821 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga
     2881 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt
     2941 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta
     3001 cggttcctgg ccttttgctg gccttgaagc tgtccctgat ggtcgtcatc tacctgcctg
     3061 gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg
     3121 gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc
     3181 ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag
     3241 ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg
     3301 tgaatccgtt agcgaggtgc cgccctgctt catccccgtg gcccgttgct cgcgtttgct
     3361 ggcggtgtcc ccggaagaaa tatatttgca tgtctttagt tctatgatga cacaaacccc
     3421 gcccagcgtc ttgtcattgg cgaattcgaa cacgcagatg cagtcggggc ggcgcggtcc
     3481 gaggtccact tcgcatatta aggtgacgcg tgtggcctcg aacaccgagc gaccctgcag
     3541 cgacccgctt aacagcgtca acagcgtgcc gcagatcccg gggggcaatg agatatgaaa
     3601 aagcctgaac tcaccgcgac gtctgtcgag aagtttctga tcgaaaagtt cgacagcgtc
     3661 tccgacctga tgcagctctc ggagggcgaa gaatctcgtg ctttcagctt cgatgtagga
     3721 gggcgtggat atgtcctgcg ggtaaatagc tgcgccgatg gtttctacaa agatcgttat
     3781 gtttatcggc actttgcatc ggccgcgctc ccgattccgg aagtgcttga cattggggaa
     3841 ttcagcgaga gcctgaccta ttgcatctcc cgccgtgcac agggtgtcac gttgcaagac
     3901 ctgcctgaaa ccgaactgcc cgctgttctg cagccggtcg cggaggccat ggatgcgatc
     3961 gctgcggccg atcttagcca gacgagcggg ttcggcccat tcggaccgca aggaatcggt
     4021 caatacacta catggcgtga tttcatatgc gcgattgctg atccccatgt gtatcactgg
     4081 caaactgtga tggacgacac cgtcagtgcg tccgtcgcgc aggctctcga tgagctgatg
     4141 ctttgggccg aggactgccc cgaagtccgg cacctcgtgc acgcggattt cggctccaac
     4201 aatgtcctga cggacaatgg ccgcataaca gcggtcattg actggagcga ggcgatgttc
     4261 ggggattccc aatacgaggt cgccaacatc ttcttctgga ggccgtggtt ggcttgtatg
     4321 gagcagcaga cgcgctactt cgagcggagg catccggagc ttgcaggatc gccgcggctc
     4381 cgggcgtata tgctccgcat tggtcttgac caactctatc agagcttggt tgacggcaat
     4441 ttcgatgatg cagcttgggc gcagggtcga tgcgacgcaa tcgtccgatc cggagccggg
     4501 actgtcgggc gtacacaaat cgcccgcaga agcgcggccg tctggaccga tggctgtgta
     4561 gaagtactcg ccgatagtgg aaaccgacgc cccagcactc gtccggatcg ggagatgggg
     4621 gaggctaact gaaacacgga aggagacaat accggaagga acccgcgcta tgacggcaat
     4681 aaaaagacag aataaaacgc acgggtgttg ggtcgtttgt tcataaacgc ggggttcggt
     4741 cccagggctg gcactctgtc gataccccac cgagacccca ttggggccaa tacgcccgcg
     4801 tttcttcctt ttccccaccc caccccccaa gttcgggtga aggcccaggg ctcgcagcca
     4861 acgtcggggc ggcaggccct gccatagcca ctggccccgt gggttaggga cggggtcccc
     4921 catggggaat ggtttatggt tcgtgggggt tattattttg ggcgttgcgt ggggtcaggt
     4981 ccacgactgg actgagcaga cagacccatg gtttttggat ggcctgggca tggaccgcat
     5041 gtactggcgc gacacgaaca ccgggcgtct gtggctgcca aacacccccg acccccaaaa
     5101 accaccgcgc ggatttctgg cgtgccaagc tagtcgacca attctcatgt ttgacagctt
     5161 atcatcgcag atccgggcaa cgttgttgcc attgctgcag gcgcagaact ggtaggtatg
     5221 gaagatctat acattgaatc aatattggca attagccata ttagtcattg gttatatagc
     5281 ataaatcaat attggctatt ggccattgca tacgttgtat ctatatcata atatgtacat
     5341 ttatattggc tcatgtccaa tatgaccgcc atgttgacat tgattattga ctagttatta
     5401 atagtaatca attacggggt cattagttca tagcccatat atggagttcc gcgttacata
     5461 acttacggta aatggcccgc ctggctgacc gcccaacgac ccccgcccat tgacgtcaat
     5521 aatgacgtat gttcccatag taacgccaat agggactttc cattgacgtc aatgggtgga
     5581 gtatttacgg taaactgccc acttggcagt acatcaagtg tatcatatgc caagtccgcc
     5641 ccctattgac gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt acatgacctt
     5701 acgggacttt cctacttggc agtacatcta cgtattagtc atcgctatta ccatggtgat
     5761 gcggttttgg cagtacacca atgggcgtgg atagcggttt gactcacggg gatttccaag
     5821 tctccacccc attgacgtca atgggagttt gttttggcac caaaatcaac gggactttcc
     5881 aaaatgtcgt aataaccccg ccccgttgac gcaaatgggc ggtaggcgtg tacggtggga
     5941 ggtctatata agcagagctc gtttagtgaa ccgtcagatc tctagaagct gggtaccagc
     6001 tgctagcgcc accatggccg tcatggcgcc ccgaaccctc gtcctgctac tctcgggggc
     6061 cctggccctg acccagacct gggcgggctc cgactacaag gacgatgatg acaagggagg
     6121 atccaagccc cacccagcag aggggcagtg gcgggcagtg gacgtggtcc tagactgttt
     6181 cctggtgaag gacggtgcgc accgtggagc tctcgccagc agtgaggaca gggcaagggc
     6241 ctcccttgtg ctgaagcagg tgccagtgct ggacgatggc tccctggagg acttcaccga
     6301 tttccaaggg ggcacactgg cccaagatga cccacctatt atctttgagg cctcagtgga
     6361 cctggtccag attccccagg ccgaggcctt gctccatgct gactgcagtg ggaaggaggt
     6421 gacctgtgag atctcccgct actttctcca gatgacagag accactgtta agacagcagc
     6481 ttggttcatg gccaacgtgc aggtctctgg agggggacct agcatctcct tggtgatgaa
     6541 gactcccagg gtcgccaaga atgaggtgct ctggcaccca acgctgaact tgccactgag
     6601 cccccagggg actgtgcgaa ctgcagtgga gttccaggtg atgacacaga cccaatccct
     6661 gagcttcctg ctggggtcct cagcctcctt ggactgtggc ttctccatgg caccgggctt
     6721 ggacctcatc agtgtggagt ggcgactgca gcacaagggc aggggtcagt tggtgtacag
     6781 ctggaccgca gggcaggggc aggctgtgcg gaagggcgct accctggagc ctgcacaact
     6841 gggcatggcc agggatgcct ccctcaccct gcccggcctc actatacagg acgaggggac
     6901 ctacatttgc cagatcacca cctctctgta ccgagctcag cagatcatcc agctcaacat
     6961 ccaagcttcc cctaaagtac gactgagctt ggcaaacgaa gctctgctgc ccaccctcat
     7021 ctgcgacatt gctggctatt accctctgga tgtggtggtg acgtggaccc gagaggagct
     7081 gggtggatcc ccagcccaag tctctggtgc ctccttctcc agcctcaggc aaagcgtggc
     7141 aggcacctac agcatctcct cctctctcac cgcagaacct ggctctgcag gtgccactta
     7201 cacctgccag gtcacacaca tctctctgga ggagcccctt ggggccagca cccaggttgt
     7261 cccaccagag cggagatcag ggccctccct tgaggacagc gtaggccttt tcctgtctgc
     7321 ctttcttctg cttgggctct tcaaggcact gggctgggct gctgtctacc tgtccacctg
     7381 caaggattca aagaaggtac agtgctccac ctctctgtat ctttcccttg tcactttatc
     7441 tcctcatcct atctcaaaac ccatggaggg aggctgctgg tgtggtaggc agaacctagg
     7501 cttggaattc acactgatct gggttaaaac ctggcactat atcctaactg taggactctt
     7561 tgagcatgct acttaatgac tcgaggccgg caaggccgga tccagacatg ataagataca
     7621 ttgatgagtt tggacaaacc acaactagaa tgcagtgaaa aaaatgcttt atttgtgaaa
     7681 tttgtgatgc tattgcttta tttgtaacca ttataagctg caataaacaa gttaacaaca
     7741 acaattgcat tcattttatg tttcaggttc agggggaggt gtgggaggtt ttttaaagca
     7801 agtaaaacct ctacaaatgt ggtatggctg attatgatcc ggctgcctcg cgcgtttcgg
     7861 tgatgacggt gaaaacctct gacacatgca gctcccggag acggtcacag cttgtctgta
     7921 agcggatgcc gggagcagac aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg
     7981 gggcgcagcc atgaggtcga ctctagagga tcgatccccg ccccggacga actaaacctg
     8041 actacgacat ctctgcccct tcttcgcggg gcagtgcatg taatcccttc agttggttgg
     8101 tacaacttgc caactgggcc ctgttccaca tgtgacacgg ggggggacca aacacaaagg
     8161 ggttctctga ctgtagttga catccttata aatggatgtg cacatttgcc aacactgagt
     8221 ggctttcatc ctggagcaga ctttgcagtc tgtggactgc aacacaacat tgcctttatg
     8281 tgtaactctt ggctgaagct cttacaccaa tgctggggga catgtacctc ccaggggccc
     8341 aggaagacta cgggaggcta caccaacgtc aatcagaggg gcctgtgtag ctaccgataa
     8401 gcggaccctc aagagggcat tagcaatagt gtttataagg cccccttgtt aaccctaaac
     8461 gggtagcata tgcttcccgg gtagtagtat atactatcca gactaaccct aattcaatag
     8521 catatgttac ccaacgggaa gcatatgcta tcgaattagg gttagtaaaa gggtcctaag
     8581 gaacagcgat atctcccacc ccatgagctg tcacggtttt atttacatgg ggtcaggatt
     8641 ccacgagggt agtgaaccat tttagtcaca agggcagtgg ctgaagatca aggagcgggc
     8701 agtgaactct cctgaatctt cgcctgcttc ttcattctcc ttcgtttagc taatagaata
     8761 actgctgagt tgtgaacagt aaggtgtatg tgaggtgctc gaaaacaagg tttcaggtga
     8821 cgcccccaga ataaaatttg gacggggggt tcagtggtgg cattgtgcta tgacaccaat
     8881 ataaccctca caaacccctt gggcaataaa tactagtgta ggaatgaaac attctgaata
     8941 tctttaacaa tagaaatcca tggggtgggg acaagccgta aagactggat gtccatctca
     9001 cacgaattta tggctatggg caacacataa tcctagtgca atatgatact ggggttatta
     9061 agatgtgtcc caggcaggga ccaagacagg tgaaccatgt tgttacactc tatttgtaac
     9121 aaggggaaag agagtggacg ccgacagcag cggactccac tggttgtctc taacaccccc
     9181 gaaaattaaa cggggctcca cgccaatggg gcccataaac aaagacaagt ggccactctt
     9241 ttttttgaaa ttgtggagtg ggggcacgcg tcagccccca cacgccgccc tgcggttttg
     9301 gactgtaaaa taagggtgta ataacttggc t
//
