LOCUS       sequence-327923-       11107 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of FLAG-tagged TAPBPR with tapasin CT 
            deltaALAS.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 11107)
  AUTHORS   McShan AC, Devlin CA, Morozov GI, Overall SA, Moschidi D, Akella N, 
            Procko E, Sgourakis NG
  TITLE     TAPBPR promotes antigen loading on MHC-I molecules using a peptide 
            trap.
  JOURNAL   Nat Commun. 2021 May 26;12(1):3174. doi: 10.1038/s41467-021-23225-6.
  PUBMED    34039964
REFERENCE   2  (bases 1 to 11107)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2021-05-26"; volume: "12(1):3174. doi"; pages: " 
            10.1038/s41467-021-23225-6"
COMMENT     Sequence Label: pCEP4-FLAG-TAPBPR-tapasinCT deltaALAS
FEATURES             Location/Qualifiers
     source          1..11107
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     rep_origin      1342..3131
                     /label=oriP
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
     primer_bind     3691..3713
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     polyA_signal    3856..3990
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3893..3912)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3947..3966
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     CDS             complement(5462..5485)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     regulatory      5560..5569
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(5597..5621)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        complement(5618..5821)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(5647..5667)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        5822..6201
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     polyA_signal    6855..6903
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     6928..6947
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     CDS             complement(6945..7982)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPDRE
                     MGEAN"
     promoter        complement(8025..8170)
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     rep_origin      complement(8601..9189)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8681..8700)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9360..10151,10152..10220))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9983..10002
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10221..10325)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     10393..10411
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 gaacaggagc aggagcagga gcgggagggg caggagcagg aggggcagga gcaggaggag
       61 gggcaggagc aggaggggca ggagcaggag gtggaggccg gggtcgagga ggcagtggag
      121 gccggggtcg aggaggtagt ggaggccggg gtcgaggagg tagtggaggc cgccggggta
      181 gaggacgtga aagagccagg gggggaagtc gtgaaagagc cagggggaga ggtcgtggac
      241 gtggagaaaa gaggcccagg agtcccagta gtcagtcatc atcatccggg tctccaccgc
      301 gcaggccccc tccaggtaga aggccatttt tccaccctgt aggggaagcc gattattttg
      361 aataccacca agaaggtggc ccagatggtg agcctgacgt gcccccggga gcgatagagc
      421 agggccccgc agatgaccca ggagaaggcc caagcactgg accccggggt cagggtgatg
      481 gaggcaggcg caaaaaagga gggtggtttg gaaagcatcg tggtcaagga ggttccaacc
      541 cgaaatttga gaacattgca gaaggtttaa gagctctcct ggctaggagt cacgtagaaa
      601 ggactaccga cgaaggaact tgggtcgccg gtgtgttcgt atatggaggt agtaagacct
      661 ccctttacaa cctaaggcga ggaactgccc ttgctattcc acaatgtcgt cttacaccat
      721 tgagtcgtct cccctttgga atggcccctg gacccggccc acaacctggc ccgctaaggg
      781 agtccattgt ctgttatttc atggtctttt tacaaactca tatatttgct gaggttttga
      841 aggatgcgat taaggacctt gttatgacaa agcccgctcc tacctgcaat atcagggtga
      901 ctgtgtgcag ctttgacgat ggagtagatt tgcctccctg gtttccacct atggtggaag
      961 gggctgccgc ggagggtgat gacggagatg acggagatga aggaggtgat ggagatgagg
     1021 gtgaggaagg gcaggagtga tgtaacttgt taggagacgc cctcaatcgt attaaaagcc
     1081 gtgtattccc ccgcactaaa gaataaatcc ccagtagaca tcatgcgtgc tgttggtgta
     1141 tttctggcca tctgtcttgt caccattttc gtcctcccaa catggggcaa ttgggcatac
     1201 ccatgttgtc acgtcactca gctccgcgct caacaccttc tcgcgttgga aaacattagc
     1261 gacatttacc tggtgagcaa tcagacatgc gacggcttta gcctggcctc cttaaattca
     1321 cctaagaatg ggagcaacca gcaggaaaag gacaagcagc gaaaattcac gcccccttgg
     1381 gaggtggcgg catatgcaaa ggatagcact cccactctac tactgggtat catatgctga
     1441 ctgtatatgc atgaggatag catatgctac ccggatacag attaggatag catatactac
     1501 ccagatatag attaggatag catatgctac ccagatatag attaggatag cctatgctac
     1561 ccagatataa attaggatag catatactac ccagatatag attaggatag catatgctac
     1621 ccagatatag attaggatag cctatgctac ccagatatag attaggatag catatgctac
     1681 ccagatatag attaggatag catatgctat ccagatattt gggtagtata tgctacccag
     1741 atataaatta ggatagcata tactacccta atctctatta ggatagcata tgctacccgg
     1801 atacagatta ggatagcata tactacccag atatagatta ggatagcata tgctacccag
     1861 atatagatta ggatagccta tgctacccag atataaatta ggatagcata tactacccag
     1921 atatagatta ggatagcata tgctacccag atatagatta ggatagccta tgctacccag
     1981 atatagatta ggatagcata tgctatccag atatttgggt agtatatgct acccatggca
     2041 acattagccc accgtgctct cagcgacctc gtgaatatga ggaccaacaa ccctgtgctt
     2101 ggcgctcagg cgcaagtgtg tgtaatttgt cctccagatc gcagcaatcg cgcccctatc
     2161 ttggcccgcc cacctactta tgcaggtatt ccccggggtg ccattagtgg ttttgtgggc
     2221 aagtggtttg accgcagtgg ttagcggggt tacaatcagc caagttatta cacccttatt
     2281 ttacagtcca aaaccgcagg gcggcgtgtg ggggctgacg cgtgccccca ctccacaatt
     2341 tcaaaaaaaa gagtggccac ttgtctttgt ttatgggccc cattggcgtg gagccccgtt
     2401 taattttcgg gggtgttaga gacaaccagt ggagtccgct gctgtcggcg tccactctct
     2461 ttccccttgt tacaaataga gtgtaacaac atggttcacc tgtcttggtc cctgcctggg
     2521 acacatctta ataaccccag tatcatattg cactaggatt atgtgttgcc catagccata
     2581 aattcgtgtg agatggacat ccagtcttta cggcttgtcc ccaccccatg gatttctatt
     2641 gttaaagata ttcagaatgt ttcattccta cactagtatt tattgcccaa ggggtttgtg
     2701 agggttatat tggtgtcata gcacaatgcc accactgaac cccccgtcca aattttattc
     2761 tgggggcgtc acctgaaacc ttgttttcga gcacctcaca tacaccttac tgttcacaac
     2821 tcagcagtta ttctattagc taaacgaagg agaatgaaga agcaggcgaa gattcaggag
     2881 agttcactgc ccgctccttg atcttcagcc actgcccttg tgactaaaat ggttcactac
     2941 cctcgtggaa tcctgacccc atgtaaataa aaccgtgaca gctcatgggg tgggagatat
     3001 cgctgttcct taggaccctt ttactaaccc taattcgata gcatatgctt cccgttgggt
     3061 aacatatgct attgaattag ggttagtctg gatagtatat actactaccc gggaagcata
     3121 tgctacccgt ttagggttaa caagggggcc ttataaacac tattgctaat gccctcttga
     3181 gggtccgctt atcggtagct acacaggccc ctctgattga cgttggtgta gcctcccgta
     3241 gtcttcctgg gcccctggga ggtacatgtc ccccagcatt ggtgtaagag cttcagccaa
     3301 gagttacaca taaaggcaat gttgtgttgc agtccacaga ctgcaaagtc tgctccagga
     3361 tgaaagccac tcagtgttgg caaatgtgca catccattta taaggatgtc aactacagtc
     3421 agagaacccc tttgtgtttg gtcccccccc gtgtcacatg tggaacaggg cccagttggc
     3481 aagttgtacc aaccaactga agggattaca tgcactgccc cgcgaagaag gggcagagat
     3541 gtcgtagtca ggtttagttc gtccggggcg gggatcgatc ctctagagtc gacctcatgg
     3601 ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg
     3661 catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac
     3721 cgtcatcacc gaaacgcgcg aggcagccgg atcataatca gccataccac atttgtagag
     3781 gttttacttg ctttaaaaaa cctcccacac ctccccctga acctgaaaca taaaatgaat
     3841 gcaattgttg ttgttaactt gtttattgca gcttataatg gttacaaata aagcaatagc
     3901 atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa
     3961 ctcatcaatg tatcttatca tgtctggatc cggccttgcc ggcctcgagt cattaagtag
     4021 catgctcaaa gagtcctaca gttaggatat agtgccaggt tttaacccag atcagtgtga
     4081 attccaagcc taggttctgc ctaccacacc agcagcctcc ctccatgggt tttgagatag
     4141 gatgaggaga taaagtgaca agggaaagat acagagaggt ggagcactgt accttctttg
     4201 aatccttgca ggtggacagg tagacagcag cccagcccag tgccttgaag agcccaagca
     4261 gaagaaaggc agacaggaaa aggcctacgc tgtcctcaag ggagggccct gatctccgct
     4321 ctggtgggac aacctgggtg ctggccccaa ggggctcctc cagagagatg tgtgtgacct
     4381 ggcaggtgta agtggcacct gcagagccag gttctgcggt gagagaggag gagatgctgt
     4441 aggtgcctgc cacgctttgc ctgaggctgg agaaggaggc accagagact tgggctgggg
     4501 atccacccag ctcctctcgg gtccacgtca ccaccacatc cagagggtaa tagccagcaa
     4561 tgtcgcagat gagggtgggc agcagagctt cgtttgccaa gctcagtcgt actttagggg
     4621 aagcttggat gttgagctgg atgatctgct gagctcggta cagagaggtg gtgatctggc
     4681 aaatgtaggt cccctcgtcc tgtatagtga ggccgggcag ggtgagggag gcatccctgg
     4741 ccatgcccag ttgtgcaggc tccagggtag cgcccttccg cacagcctgc ccctgccctg
     4801 cggtccagct gtacaccaac tgacccctgc ccttgtgctg cagtcgccac tccacactga
     4861 tgaggtccaa gcccggtgcc atggagaagc cacagtccaa ggaggctgag gaccccagca
     4921 ggaagctcag ggattgggtc tgtgtcatca cctggaactc cactgcagtt cgcacagtcc
     4981 cctgggggct cagtggcaag ttcagcgttg ggtgccagag cacctcattc ttggcgaccc
     5041 tgggagtctt catcaccaag gagatgctag gtccccctcc agagacctgc acgttggcca
     5101 tgaaccaagc tgctgtctta acagtggtct ctgtcatctg gagaaagtag cgggagatct
     5161 cacaggtcac ctccttccca ctgcagtcag catggagcaa ggcctcggcc tggggaatct
     5221 ggaccaggtc cactgaggcc tcaaagataa taggtgggtc atcttgggcc agtgtgcccc
     5281 cttggaaatc ggtgaagtcc tccagggagc catcgtccag cactggcacc tgcttcagca
     5341 caagggaggc ccttgccctg tcctcacttc cacggtgcgc accgtccttc accaggaaac
     5401 agtctaggac cacgtccact gcccgccact gcccctctgc tgggtggggc ttggatcctc
     5461 ccttgtcatc atcgtccttg tagtcggagc ccgcccaggt ctgggtcagg gccagggccc
     5521 ccgagagtag caggacgagg gttcggggcg ccatgacggc catggtggcg ctagcagctg
     5581 gtacccagct tctagagatc tgacggttca ctaaacgagc tctgcttata tagacctccc
     5641 accgtacacg cctaccgccc atttgcgtca acggggcggg gttattacga cattttggaa
     5701 agtcccgttg attttggtgc caaaacaaac tcccattgac gtcaatgggg tggagacttg
     5761 gaaatccccg tgagtcaaac cgctatccac gcccattggt gtactgccaa aaccgcatca
     5821 ccatggtaat agcgatgact aatacgtaga tgtactgcca agtaggaaag tcccgtaagg
     5881 tcatgtactg ggcataatgc caggcgggcc atttaccgtc attgacgtca atagggggcg
     5941 gacttggcat atgatacact tgatgtactg ccaagtgggc agtttaccgt aaatactcca
     6001 cccattgacg tcaatggaaa gtccctattg gcgttactat gggaacatac gtcattattg
     6061 acgtcaatgg gcgggggtcg ttgggcggtc agccaggcgg gccatttacc gtaagttatg
     6121 taacgcggaa ctccatatat gggctatgaa ctaatgaccc cgtaattgat tactattaat
     6181 aactagtcaa taatcaatgt caacatggcg gtcatattgg acatgagcca atataaatgt
     6241 acatattatg atatagatac aacgtatgca atggccaata gccaatattg atttatgcta
     6301 tataaccaat gactaatatg gctaattgcc aatattgatt caatgtatag atcttccata
     6361 cctaccagtt ctgcgcctgc agcaatggca acaacgttgc ccggatctgc gatgataagc
     6421 tgtcaaacat gagaattggt cgactagctt ggcacgccag aaatccgcgc ggtggttttt
     6481 gggggtcggg ggtgtttggc agccacagac gcccggtgtt cgtgtcgcgc cagtacatgc
     6541 ggtccatgcc caggccatcc aaaaaccatg ggtctgtctg ctcagtccag tcgtggacct
     6601 gaccccacgc aacgcccaaa ataataaccc ccacgaacca taaaccattc cccatggggg
     6661 accccgtccc taacccacgg ggccagtggc tatggcaggg cctgccgccc cgacgttggc
     6721 tgcgagccct gggccttcac ccgaacttgg ggggtggggt ggggaaaagg aagaaacgcg
     6781 ggcgtattgg ccccaatggg gtctcggtgg ggtatcgaca gagtgccagc cctgggaccg
     6841 aaccccgcgt ttatgaacaa acgacccaac acccgtgcgt tttattctgt ctttttattg
     6901 ccgtcatagc gcgggttcct tccggtattg tctccttccg tgtttcagtt agcctccccc
     6961 atctcccgat ccggacgagt gctggggcgt cggtttccac tatcggcgag tacttctaca
     7021 cagccatcgg tccagacggc cgcgcttctg cgggcgattt gtgtacgccc gacagtcccg
     7081 gctccggatc ggacgattgc gtcgcatcga ccctgcgccc aagctgcatc atcgaaattg
     7141 ccgtcaacca agctctgata gagttggtca agaccaatgc ggagcatata cgcccggagc
     7201 cgcggcgatc ctgcaagctc cggatgcctc cgctcgaagt agcgcgtctg ctgctccata
     7261 caagccaacc acggcctcca gaagaagatg ttggcgacct cgtattggga atccccgaac
     7321 atcgcctcgc tccagtcaat gaccgctgtt atgcggccat tgtccgtcag gacattgttg
     7381 gagccgaaat ccgcgtgcac gaggtgccgg acttcggggc agtcctcggc ccaaagcatc
     7441 agctcatcga gagcctgcgc gacggacgca ctgacggtgt cgtccatcac agtttgccag
     7501 tgatacacat ggggatcagc aatcgcgcat atgaaatcac gccatgtagt gtattgaccg
     7561 attccttgcg gtccgaatgg gccgaacccg ctcgtctggc taagatcggc cgcagcgatc
     7621 gcatccatgg cctccgcgac cggctgcaga acagcgggca gttcggtttc aggcaggtct
     7681 tgcaacgtga caccctgtgc acggcgggag atgcaatagg tcaggctctc gctgaattcc
     7741 ccaatgtcaa gcacttccgg aatcgggagc gcggccgatg caaagtgccg ataaacataa
     7801 cgatctttgt agaaaccatc ggcgcagcta tttacccgca ggacatatcc acgccctcct
     7861 acatcgaagc tgaaagcacg agattcttcg ccctccgaga gctgcatcag gtcggagacg
     7921 ctgtcgaact tttcgatcag aaacttctcg acagacgtcg cggtgagttc aggctttttc
     7981 atatctcatt gccccccggg atctgcggca cgctgttgac gctgttaagc gggtcgctgc
     8041 agggtcgctc ggtgttcgag gccacacgcg tcaccttaat atgcgaagtg gacctcggac
     8101 cgcgccgccc cgactgcatc tgcgtgttcg aattcgccaa tgacaagacg ctgggcgggg
     8161 tttgtgtcat catagaacta aagacatgca aatatatttc ttccggggac accgccagca
     8221 aacgcgagca acgggccacg gggatgaagc agggcggcac ctcgctaacg gattcaccac
     8281 tccaagaatt ggagccaatc aattcttgcg gagaactgtg aatgcgcaaa ccaacccttg
     8341 gcagaacata tccatcgcgt ccgccatctc cagcagccgc acgcggcgca tctcggggcc
     8401 gacgcgctgg gctacgtctt gctggcgttc gcgacgcgag gctggatggc cttccccatt
     8461 atgattcttc tcgcttccgg cggcatcggg atgcccgcgt tgcaggccat gctgtccagg
     8521 caggtagatg acgaccatca gggacagctt caaggccagc aaaaggccag gaaccgtaaa
     8581 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
     8641 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
     8701 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
     8761 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt
     8821 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
     8881 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
     8941 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
     9001 gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc
     9061 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
     9121 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
     9181 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac
     9241 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta
     9301 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt
     9361 taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata
     9421 gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc
     9481 agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac
     9541 cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag
     9601 tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac
     9661 gttgttgcca ttgctgcagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc
     9721 agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg
     9781 gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc
     9841 atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct
     9901 gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc
     9961 tcttgcccgg cgtcaacacg ggataatacc gcgccacata gcagaacttt aaaagtgctc
    10021 atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc
    10081 agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc
    10141 gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca
    10201 cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt
    10261 tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt
    10321 ccgcgcacat ttccccgaaa agtgccacct gacgtctaag aaaccattat tatcatgaca
    10381 ttaacctata aaaataggcg tatcacgagg ccctttcgtc ttcaagaatt ctcatgtttg
    10441 acagcttatc atcgataagc tgatcctcac aggccgcacc cagcttttct tccgttgccc
    10501 cagtagcatc tctgtctggt gaccttgaag aggaagagga ggggtcccga gaatccccat
    10561 ccctaccgtc cagcaaaaag ggggacgagg aatttgaggc ctggcttgag gctcaggacg
    10621 caaatcttga ggatgttcag cgggagtttt ccgggctgcg agtaattggt gatgaggacg
    10681 aggatggttc ggaggatggg gaattttcag acctggatct gtctgacagc gaccatgaag
    10741 gggatgaggg tgggggggct gttggagggg gcaggagtct gcactccctg tattcactga
    10801 gcgtcgtcta ataaagatgt ctattgatct cttttagtgt gaatcatgtc tgacgagggg
    10861 ccaggtacag gacctggaaa tggcctagga gagaagggag acacatctgg accagaaggc
    10921 tccggcggca gtggacctca aagaagaggg ggtgataacc atggacgagg acggggaaga
    10981 ggacgaggac gaggaggcgg aagaccagga gccccgggcg gctcaggatc agggccaaga
    11041 catagagatg gtgtccggag accccaaaaa cgtccaagtt gcattggctg caaagggacc
    11101 cacggtg
//
