LOCUS       sequence-327925-       11119 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of FLAG-tagged TAPBPR with tapasin CT E102A.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 11119)
  AUTHORS   McShan AC, Devlin CA, Morozov GI, Overall SA, Moschidi D, Akella N, 
            Procko E, Sgourakis NG
  TITLE     TAPBPR promotes antigen loading on MHC-I molecules using a peptide 
            trap.
  JOURNAL   Nat Commun. 2021 May 26;12(1):3174. doi: 10.1038/s41467-021-23225-6.
  PUBMED    34039964
REFERENCE   2  (bases 1 to 11119)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2021-05-26"; volume: "12(1):3174. doi"; pages: " 
            10.1038/s41467-021-23225-6"
COMMENT     Sequence Label: pCEP4-FLAG-TAPBPR-tapasinCT E102A
FEATURES             Location/Qualifiers
     source          1..11119
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     rep_origin      1318..3107
                     /label=oriP
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
     primer_bind     3667..3689
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     polyA_signal    3832..3966
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3869..3888)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3923..3942
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     CDS             complement(5450..5473)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     regulatory      5548..5557
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(5585..5609)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        complement(5606..5809)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(5635..5655)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        5810..6189
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     polyA_signal    6843..6891
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     6916..6935
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     CDS             complement(6933..7970)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPDRE
                     MGEAN"
     promoter        complement(8013..8158)
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     rep_origin      complement(8589..9177)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8669..8688)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9348..10139,10140..10208))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9971..9990
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10209..10313)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     10381..10399
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 gaggggcagg agcaggaggg gcaggagcag gaggaggggc aggagcagga ggggcaggag
       61 caggaggtgg aggccggggt cgaggaggca gtggaggccg gggtcgagga ggtagtggag
      121 gccggggtcg aggaggtagt ggaggccgcc ggggtagagg acgtgaaaga gccagggggg
      181 gaagtcgtga aagagccagg gggagaggtc gtggacgtgg agaaaagagg cccaggagtc
      241 ccagtagtca gtcatcatca tccgggtctc caccgcgcag gccccctcca ggtagaaggc
      301 catttttcca ccctgtaggg gaagccgatt attttgaata ccaccaagaa ggtggcccag
      361 atggtgagcc tgacgtgccc ccgggagcga tagagcaggg ccccgcagat gacccaggag
      421 aaggcccaag cactggaccc cggggtcagg gtgatggagg caggcgcaaa aaaggagggt
      481 ggtttggaaa gcatcgtggt caaggaggtt ccaacccgaa atttgagaac attgcagaag
      541 gtttaagagc tctcctggct aggagtcacg tagaaaggac taccgacgaa ggaacttggg
      601 tcgccggtgt gttcgtatat ggaggtagta agacctccct ttacaaccta aggcgaggaa
      661 ctgcccttgc tattccacaa tgtcgtctta caccattgag tcgtctcccc tttggaatgg
      721 cccctggacc cggcccacaa cctggcccgc taagggagtc cattgtctgt tatttcatgg
      781 tctttttaca aactcatata tttgctgagg ttttgaagga tgcgattaag gaccttgtta
      841 tgacaaagcc cgctcctacc tgcaatatca gggtgactgt gtgcagcttt gacgatggag
      901 tagatttgcc tccctggttt ccacctatgg tggaaggggc tgccgcggag ggtgatgacg
      961 gagatgacgg agatgaagga ggtgatggag atgagggtga ggaagggcag gagtgatgta
     1021 acttgttagg agacgccctc aatcgtatta aaagccgtgt attcccccgc actaaagaat
     1081 aaatccccag tagacatcat gcgtgctgtt ggtgtatttc tggccatctg tcttgtcacc
     1141 attttcgtcc tcccaacatg gggcaattgg gcatacccat gttgtcacgt cactcagctc
     1201 cgcgctcaac accttctcgc gttggaaaac attagcgaca tttacctggt gagcaatcag
     1261 acatgcgacg gctttagcct ggcctcctta aattcaccta agaatgggag caaccagcag
     1321 gaaaaggaca agcagcgaaa attcacgccc ccttgggagg tggcggcata tgcaaaggat
     1381 agcactccca ctctactact gggtatcata tgctgactgt atatgcatga ggatagcata
     1441 tgctacccgg atacagatta ggatagcata tactacccag atatagatta ggatagcata
     1501 tgctacccag atatagatta ggatagccta tgctacccag atataaatta ggatagcata
     1561 tactacccag atatagatta ggatagcata tgctacccag atatagatta ggatagccta
     1621 tgctacccag atatagatta ggatagcata tgctacccag atatagatta ggatagcata
     1681 tgctatccag atatttgggt agtatatgct acccagatat aaattaggat agcatatact
     1741 accctaatct ctattaggat agcatatgct acccggatac agattaggat agcatatact
     1801 acccagatat agattaggat agcatatgct acccagatat agattaggat agcctatgct
     1861 acccagatat aaattaggat agcatatact acccagatat agattaggat agcatatgct
     1921 acccagatat agattaggat agcctatgct acccagatat agattaggat agcatatgct
     1981 atccagatat ttgggtagta tatgctaccc atggcaacat tagcccaccg tgctctcagc
     2041 gacctcgtga atatgaggac caacaaccct gtgcttggcg ctcaggcgca agtgtgtgta
     2101 atttgtcctc cagatcgcag caatcgcgcc cctatcttgg cccgcccacc tacttatgca
     2161 ggtattcccc ggggtgccat tagtggtttt gtgggcaagt ggtttgaccg cagtggttag
     2221 cggggttaca atcagccaag ttattacacc cttattttac agtccaaaac cgcagggcgg
     2281 cgtgtggggg ctgacgcgtg cccccactcc acaatttcaa aaaaaagagt ggccacttgt
     2341 ctttgtttat gggccccatt ggcgtggagc cccgtttaat tttcgggggt gttagagaca
     2401 accagtggag tccgctgctg tcggcgtcca ctctctttcc ccttgttaca aatagagtgt
     2461 aacaacatgg ttcacctgtc ttggtccctg cctgggacac atcttaataa ccccagtatc
     2521 atattgcact aggattatgt gttgcccata gccataaatt cgtgtgagat ggacatccag
     2581 tctttacggc ttgtccccac cccatggatt tctattgtta aagatattca gaatgtttca
     2641 ttcctacact agtatttatt gcccaagggg tttgtgaggg ttatattggt gtcatagcac
     2701 aatgccacca ctgaaccccc cgtccaaatt ttattctggg ggcgtcacct gaaaccttgt
     2761 tttcgagcac ctcacataca ccttactgtt cacaactcag cagttattct attagctaaa
     2821 cgaaggagaa tgaagaagca ggcgaagatt caggagagtt cactgcccgc tccttgatct
     2881 tcagccactg cccttgtgac taaaatggtt cactaccctc gtggaatcct gaccccatgt
     2941 aaataaaacc gtgacagctc atggggtggg agatatcgct gttccttagg acccttttac
     3001 taaccctaat tcgatagcat atgcttcccg ttgggtaaca tatgctattg aattagggtt
     3061 agtctggata gtatatacta ctacccggga agcatatgct acccgtttag ggttaacaag
     3121 ggggccttat aaacactatt gctaatgccc tcttgagggt ccgcttatcg gtagctacac
     3181 aggcccctct gattgacgtt ggtgtagcct cccgtagtct tcctgggccc ctgggaggta
     3241 catgtccccc agcattggtg taagagcttc agccaagagt tacacataaa ggcaatgttg
     3301 tgttgcagtc cacagactgc aaagtctgct ccaggatgaa agccactcag tgttggcaaa
     3361 tgtgcacatc catttataag gatgtcaact acagtcagag aacccctttg tgtttggtcc
     3421 ccccccgtgt cacatgtgga acagggccca gttggcaagt tgtaccaacc aactgaaggg
     3481 attacatgca ctgccccgcg aagaaggggc agagatgtcg tagtcaggtt tagttcgtcc
     3541 ggggcgggga tcgatcctct agagtcgacc tcatggctgc gccccgacac ccgccaacac
     3601 ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga
     3661 ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc
     3721 agccggatca taatcagcca taccacattt gtagaggttt tacttgcttt aaaaaacctc
     3781 ccacacctcc ccctgaacct gaaacataaa atgaatgcaa ttgttgttgt taacttgttt
     3841 attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca
     3901 tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc
     3961 tggatccggc cttgccggcc tcgagtcatt aagtagcatg ctcaaagagt cctacagtta
     4021 ggatatagtg ccaggtttta acccagatca gtgtgaattc caagcctagg ttctgcctac
     4081 cacaccagca gcctccctcc atgggttttg agataggatg aggagataaa gtgacaaggg
     4141 aaagatacag agaggtggag cactgtacct tctttgaatc cttgcaggtg gacaggtaga
     4201 cagcagccca gcccagtgcc ttgaagagcc caagcagaag aaaggcagac aggaaaaggc
     4261 ctacgctgtc ctcaagggag ggccctgatc tccgctctgg tgggacaacc tgggtgctgg
     4321 ccccaagggg ctcctccaga gagatgtgtg tgacctggca ggtgtaagtg gcacctgcag
     4381 agccaggttc tgcggtgaga gaggaggaga tgctgtaggt gcctgccacg ctttgcctga
     4441 ggctggagaa ggaggcacca gagacttggg ctggggatcc acccagctcc tctcgggtcc
     4501 acgtcaccac cacatccaga gggtaatagc cagcaatgtc gcagatgagg gtgggcagca
     4561 gagcttcgtt tgccaagctc agtcgtactt taggggaagc ttggatgttg agctggatga
     4621 tctgctgagc tcggtacaga gaggtggtga tctggcaaat gtaggtcccc tcgtcctgta
     4681 tagtgaggcc gggcagggtg agggaggcat ccctggccat gcccagttgt gcaggctcca
     4741 gggtagcgcc cttccgcaca gcctgcccct gccctgcggt ccagctgtac accaactgac
     4801 ccctgccctt gtgctgcagt cgccactcca cactgatgag gtccaagccc ggtgccatgg
     4861 agaagccaca gtccaaggag gctgaggacc ccagcaggaa gctcagggat tgggtctgtg
     4921 tcatcacctg gaactccact gcagttcgca cagtcccctg ggggctcagt ggcaagttca
     4981 gcgttgggtg ccagagcacc tcattcttgg cgaccctggg agtcttcatc accaaggaga
     5041 tgctaggtcc ccctccagag acctgcacgt tggccatgaa ccaagctgct gtcttaacag
     5101 tggtctctgt catctggaga aagtagcggg agatagcaca ggtcacctcc ttcccactgc
     5161 agtcagcatg gagcaaggcc tcggcctggg gaatctggac caggtccact gaggcctcaa
     5221 agataatagg tgggtcatct tgggccagtg tgcccccttg gaaatcggtg aagtcctcca
     5281 gggagccatc gtccagcact ggcacctgct tcagcacaag ggaggccctt gccctgtcct
     5341 cactgctggc gagagctcca cggtgcgcac cgtccttcac caggaaacag tctaggacca
     5401 cgtccactgc ccgccactgc ccctctgctg ggtggggctt ggatcctccc ttgtcatcat
     5461 cgtccttgta gtcggagccc gcccaggtct gggtcagggc cagggccccc gagagtagca
     5521 ggacgagggt tcggggcgcc atgacggcca tggtggcgct agcagctggt acccagcttc
     5581 tagagatctg acggttcact aaacgagctc tgcttatata gacctcccac cgtacacgcc
     5641 taccgcccat ttgcgtcaac ggggcggggt tattacgaca ttttggaaag tcccgttgat
     5701 tttggtgcca aaacaaactc ccattgacgt caatggggtg gagacttgga aatccccgtg
     5761 agtcaaaccg ctatccacgc ccattggtgt actgccaaaa ccgcatcacc atggtaatag
     5821 cgatgactaa tacgtagatg tactgccaag taggaaagtc ccgtaaggtc atgtactggg
     5881 cataatgcca ggcgggccat ttaccgtcat tgacgtcaat agggggcgga cttggcatat
     5941 gatacacttg atgtactgcc aagtgggcag tttaccgtaa atactccacc cattgacgtc
     6001 aatggaaagt ccctattggc gttactatgg gaacatacgt cattattgac gtcaatgggc
     6061 gggggtcgtt gggcggtcag ccaggcgggc catttaccgt aagttatgta acgcggaact
     6121 ccatatatgg gctatgaact aatgaccccg taattgatta ctattaataa ctagtcaata
     6181 atcaatgtca acatggcggt catattggac atgagccaat ataaatgtac atattatgat
     6241 atagatacaa cgtatgcaat ggccaatagc caatattgat ttatgctata taaccaatga
     6301 ctaatatggc taattgccaa tattgattca atgtatagat cttccatacc taccagttct
     6361 gcgcctgcag caatggcaac aacgttgccc ggatctgcga tgataagctg tcaaacatga
     6421 gaattggtcg actagcttgg cacgccagaa atccgcgcgg tggtttttgg gggtcggggg
     6481 tgtttggcag ccacagacgc ccggtgttcg tgtcgcgcca gtacatgcgg tccatgccca
     6541 ggccatccaa aaaccatggg tctgtctgct cagtccagtc gtggacctga ccccacgcaa
     6601 cgcccaaaat aataaccccc acgaaccata aaccattccc catgggggac cccgtcccta
     6661 acccacgggg ccagtggcta tggcagggcc tgccgccccg acgttggctg cgagccctgg
     6721 gccttcaccc gaacttgggg ggtggggtgg ggaaaaggaa gaaacgcggg cgtattggcc
     6781 ccaatggggt ctcggtgggg tatcgacaga gtgccagccc tgggaccgaa ccccgcgttt
     6841 atgaacaaac gacccaacac ccgtgcgttt tattctgtct ttttattgcc gtcatagcgc
     6901 gggttccttc cggtattgtc tccttccgtg tttcagttag cctcccccat ctcccgatcc
     6961 ggacgagtgc tggggcgtcg gtttccacta tcggcgagta cttctacaca gccatcggtc
     7021 cagacggccg cgcttctgcg ggcgatttgt gtacgcccga cagtcccggc tccggatcgg
     7081 acgattgcgt cgcatcgacc ctgcgcccaa gctgcatcat cgaaattgcc gtcaaccaag
     7141 ctctgataga gttggtcaag accaatgcgg agcatatacg cccggagccg cggcgatcct
     7201 gcaagctccg gatgcctccg ctcgaagtag cgcgtctgct gctccataca agccaaccac
     7261 ggcctccaga agaagatgtt ggcgacctcg tattgggaat ccccgaacat cgcctcgctc
     7321 cagtcaatga ccgctgttat gcggccattg tccgtcagga cattgttgga gccgaaatcc
     7381 gcgtgcacga ggtgccggac ttcggggcag tcctcggccc aaagcatcag ctcatcgaga
     7441 gcctgcgcga cggacgcact gacggtgtcg tccatcacag tttgccagtg atacacatgg
     7501 ggatcagcaa tcgcgcatat gaaatcacgc catgtagtgt attgaccgat tccttgcggt
     7561 ccgaatgggc cgaacccgct cgtctggcta agatcggccg cagcgatcgc atccatggcc
     7621 tccgcgaccg gctgcagaac agcgggcagt tcggtttcag gcaggtcttg caacgtgaca
     7681 ccctgtgcac ggcgggagat gcaataggtc aggctctcgc tgaattcccc aatgtcaagc
     7741 acttccggaa tcgggagcgc ggccgatgca aagtgccgat aaacataacg atctttgtag
     7801 aaaccatcgg cgcagctatt tacccgcagg acatatccac gccctcctac atcgaagctg
     7861 aaagcacgag attcttcgcc ctccgagagc tgcatcaggt cggagacgct gtcgaacttt
     7921 tcgatcagaa acttctcgac agacgtcgcg gtgagttcag gctttttcat atctcattgc
     7981 cccccgggat ctgcggcacg ctgttgacgc tgttaagcgg gtcgctgcag ggtcgctcgg
     8041 tgttcgaggc cacacgcgtc accttaatat gcgaagtgga cctcggaccg cgccgccccg
     8101 actgcatctg cgtgttcgaa ttcgccaatg acaagacgct gggcggggtt tgtgtcatca
     8161 tagaactaaa gacatgcaaa tatatttctt ccggggacac cgccagcaaa cgcgagcaac
     8221 gggccacggg gatgaagcag ggcggcacct cgctaacgga ttcaccactc caagaattgg
     8281 agccaatcaa ttcttgcgga gaactgtgaa tgcgcaaacc aacccttggc agaacatatc
     8341 catcgcgtcc gccatctcca gcagccgcac gcggcgcatc tcggggccga cgcgctgggc
     8401 tacgtcttgc tggcgttcgc gacgcgaggc tggatggcct tccccattat gattcttctc
     8461 gcttccggcg gcatcgggat gcccgcgttg caggccatgc tgtccaggca ggtagatgac
     8521 gaccatcagg gacagcttca aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
     8581 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
     8641 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
     8701 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
     8761 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc
     8821 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
     8881 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
     8941 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
     9001 tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag
     9061 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
     9121 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
     9181 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg
     9241 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga
     9301 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta
     9361 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc
     9421 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg
     9481 ataccgcgag acccacgctc accggctcca gatttatcag caataaacca gccagccgga
     9541 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt
     9601 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt
     9661 gctgcaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc
     9721 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc
     9781 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca
     9841 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag
     9901 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg
     9961 tcaacacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa
    10021 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa
    10081 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga
    10141 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga
    10201 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg
    10261 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt
    10321 ccccgaaaag tgccacctga cgtctaagaa accattatta tcatgacatt aacctataaa
    10381 aataggcgta tcacgaggcc ctttcgtctt caagaattct catgtttgac agcttatcat
    10441 cgataagctg atcctcacag gccgcaccca gcttttcttc cgttgcccca gtagcatctc
    10501 tgtctggtga ccttgaagag gaagaggagg ggtcccgaga atccccatcc ctaccgtcca
    10561 gcaaaaaggg ggacgaggaa tttgaggcct ggcttgaggc tcaggacgca aatcttgagg
    10621 atgttcagcg ggagttttcc gggctgcgag taattggtga tgaggacgag gatggttcgg
    10681 aggatgggga attttcagac ctggatctgt ctgacagcga ccatgaaggg gatgagggtg
    10741 ggggggctgt tggagggggc aggagtctgc actccctgta ttcactgagc gtcgtctaat
    10801 aaagatgtct attgatctct tttagtgtga atcatgtctg acgaggggcc aggtacagga
    10861 cctggaaatg gcctaggaga gaagggagac acatctggac cagaaggctc cggcggcagt
    10921 ggacctcaaa gaagaggggg tgataaccat ggacgaggac ggggaagagg acgaggacga
    10981 ggaggcggaa gaccaggagc cccgggcggc tcaggatcag ggccaagaca tagagatggt
    11041 gtccggagac cccaaaaacg tccaagttgc attggctgca aagggaccca cggtggaaca
    11101 ggagcaggag caggagcgg
//
