LOCUS       sequence-327934-       10309 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  DEPDC5 expressing vector for mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10309)
  AUTHORS   Lawrence RE, Fromm SA, Fu Y, Yokom AL, Kim DJ, Thelen AM, Young LN, 
            Lim CY, Samelson AJ, Hurley JH, Zoncu R
  TITLE     Structural mechanism of a Rag GTPase activation checkpoint by the 
            lysosomal folliculin complex.
  JOURNAL   Science. 2019 Nov 22;366(6468):971-977. doi: 
            10.1126/science.aax0364. Epub 2019 Oct 31.
  PUBMED    31672913
REFERENCE   2  (bases 1 to 10309)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1126/science.aax0364"; journalName: "Science"; date: 
            "2019-11-22- 22"; volume: "366"; issue: "6468"; pages: "971-977"
COMMENT     Sequence Label: pCAG GST DEPDC5
FEATURES             Location/Qualifiers
     source          1..10309
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     intron          122..1138
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1062..1084
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1146..1165
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     regulatory      1212..1221
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1218..1874
                     /codon_start=1
                     /product="glutathione S-transferase from Schistosoma 
                     japonicum"
                     /label=GST
                     /translation="MASPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNK
                     KFELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIR
                     YGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVV
                     LYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPK"
     primer_bind     1832..1854
                     /label=pGEX 5'
                     /note="pGEX vectors, Glutathione-S-transferase, forward 
                     primer"
     CDS             1902..1922
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     primer_bind     complement(6830..6849)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    6895..6950
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(6949..6968)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(7311..7327)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7311..7327)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7324..7346)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7335..7351
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7359..7365,7366..7383,7384..7389))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7404..7425
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        7484..7679
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      7530..7665
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    7685..7819
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7722..7741)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7776..7795
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7887..7904)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8058..8646)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8138..8157)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8817..9608,9609..9677))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9440..9459
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9678..9782)
                     /gene="bla"
                     /label=AmpR promoter
     enhancer        9813..10192
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 gggcggggcg aggcggagag gtgcggcggc agccaatcag agcggcgcgc tccgaaagtt
       61 tccttttatg gcgaggcggc ggcggcggcg gccctataaa aagcgaagcg cgcggcgggc
      121 gggagtcgct gcgcgctgcc ttcgccccgt gccccgctcc gccgccgcct cgcgccgccc
      181 gccccggctc tgactgaccg cgttactccc acaggtgagc gggcgggacg gcccttctcc
      241 tccgggctgt aattagcgct tggtttaatg acggcttgtt tcttttctgt ggctgcgtga
      301 aagccttgag gggctccggg agggcccttt gtgcgggggg agcggctcgg ggggtgcgtg
      361 cgtgtgtgtg tgcgtgggga gcgccgcgtg cggctccgcg ctgcccggcg gctgtgagcg
      421 ctgcgggcgc ggcgcggggc tttgtgcgct ccgcagtgtg cgcgagggga gcgcggccgg
      481 gggcggtgcc ccgcggtgcg gggggggctg cgaggggaac aaaggctgcg tgcggggtgt
      541 gtgcgtgggg gggtgagcag ggggtgtggg cgcgtcggtc gggctgcaac cccccctgca
      601 cccccctccc cgagttgctg agcacggccc ggcttcgggt gcggggctcc gtacggggcg
      661 tggcgcgggg ctcgccgtgc cgggcggggg gtggcggcag gtgggggtgc cgggcggggc
      721 ggggccgcct cgggccgggg agggctcggg ggaggggcgc ggcggccccc ggagcgccgg
      781 cggctgtcga ggcgcggcga gccgcagcca ttgcctttta tggtaatcgt gcgagagggc
      841 gcagggactt cctttgtccc aaatctgtgc ggagccgaaa tctgggaggc gccgccgcac
      901 cccctctagc gggcgcgggg cgaagcggtg cggcgccggc aggaaggaaa tgggcgggga
      961 gggccttcgt gcgtcgccgc gccgccgtcc ccttctccct ctccagcctc ggggctgtcc
     1021 gcggggggac ggctgccttc gggggggacg gggcagggcg gggttcggct tctggcgtgt
     1081 gaccggcggc tctagagcct ctgctaacca tgttcatgcc ttcttctttt tcctacagct
     1141 cctgggcaac gtgctggtta ttgtgctgtc tcatcatttt ggcaaagaat tcctcgatcg
     1201 acggtatcga tgccaccatg gcatccccta tactaggtta ttggaaaatt aagggccttg
     1261 tgcaacccac tcgacttctt ttggaatatc ttgaagaaaa atatgaagag catttgtatg
     1321 agcgcgatga aggtgataaa tggcgaaaca aaaagtttga attgggtttg gagtttccca
     1381 atcttcctta ttatattgat ggtgatgtta aattaacaca gtctatggcc atcatacgtt
     1441 atatagctga caagcacaac atgttgggtg gttgtccaaa agagcgtgca gagatttcaa
     1501 tgcttgaagg agcggttttg gatattagat acggtgtttc gagaattgca tatagtaaag
     1561 actttgaaac tctcaaagtt gattttctta gcaagctacc tgaaatgctg aaaatgttcg
     1621 aagatcgttt atgtcataaa acatatttaa atggtgatca tgtaacccat cctgacttca
     1681 tgttgtatga cgctcttgat gttgttttat acatggaccc aatgtgcctg gatgcgttcc
     1741 caaaattagt ttgttttaaa aaacgtattg aagctatccc acaaattgat aagtacttga
     1801 aatccagcaa gtatatagca tggcctttgc agggctggca agccacgttt ggtggtggcg
     1861 accatcctcc aaaatcggat gattacgata tcccaacgac cgaaaacctg tattttcagg
     1921 gtggtaccat gcggacaaca aaggtctaca aactggtcat ccataaaaaa gggttcggcg
     1981 ggagcgacga cgagctggtg gtgaatccta aggtgtttcc ccacatcaag ctgggcgata
     2041 tcgtggagat cgcccacccc aatgacgagt attctcccct gctgctgcag gtgaagtctc
     2101 tgaaggagga tctgcagaag gagacaatca gcgtggacca gacagtgacc caggtgtttc
     2161 ggctgcggcc ataccaggac gtgtatgtga acgtggtgga ccccaaggat gtgacactgg
     2221 atctggtgga gctgaccttt aaggatcagt acatcggcag aggcgatatg tggcggctga
     2281 agaagtccct ggtgtccaca tgcgcctaca tcacccagaa ggtggagttc gccggcatca
     2341 gagcccaggc cggcgagctg tgggtgaaga atgagaaggt aatgtgcggc tacatcagcg
     2401 aggatacacg ggtggtgttt cggtctacat ccgccatggt gtatatcttc atccagatgt
     2461 cctgcgagat gtgggacttc gacatctatg gcgacctgta cttcgagaag gccgtgaatg
     2521 gctttctggc cgatctgttt accaagtgga aggagaagaa ttgtagccac gaggtgacag
     2581 tggtgctgtt tagccggacc ttctatgacg ccaagtctgt ggacgagttc cctgagatca
     2641 atagagcctc tatcagacag gatcacaagg gccggtttta cgaggacttc tacaaggtgg
     2701 tggtgcagaa tgagagacgg gaggagtgga catctctgct ggtgaccatc aagaagctgt
     2761 tcatccagta cccagtgctg gtgcggctgg agcaggccga gggcttcccc cagggcgaca
     2821 attccaccag cgcccagggc aactacctgg aggccatcaa cctgtccttt aacgtgttcg
     2881 ataagcacta tatcaacaga aacttcgacc ggacaggcca gatgtccgtg gtaatcacac
     2941 ccggcgtggg cgtgtttgag gtggatagac tgctgatgat cctgaccaag cagcgcatga
     3001 tcgacaatgg catcggcgtg gatctggtgt gcatgggcga gcagcctctg cacgccgtgc
     3061 ccctgttcaa gctgcacaac aggtccgccc ccagagacag ccggctgggc gatgattaca
     3121 acatcccaca ctggatcaat cacagcttct acacctctaa gtcccagctg ttttgtaaca
     3181 gcttcacacc acggatcaag ctggccggca agaagcctgc cagcgagaag gccaagaacg
     3241 gcagagacac cagcctgggc agccctaagg agtccgagaa cgccctgcct atccaggtgg
     3301 actacgatgc ctacgacgcc caggtgttca ggctgcccgg cccatccaga gcccagtgtc
     3361 tgacaacctg cagatctgtg agagagagag agtctcactc cagaaagtcc gcctcctcct
     3421 gcgacgtgtc ctcctcccca agcctgccat cccggaccct gcccaccgag gaggtgagaa
     3481 gccaggccag cgatgattcc agcctgggca agtctgccaa tatcctgatg atcccccacc
     3541 cacacctgca ccagtatgag gtgtccagct ccctgggcta cacctccacc agagacgtgc
     3601 tggagaatat gatggagcca cctcagcggg attcttccgc cccaggcaga ttccacgtgg
     3661 gctccgccga gagcatgctg cacgtgcggc ctggcggcta caccccacag agagccctga
     3721 tcaacccatt cgccccttcc agaatgccaa tgaagctgac aagcaacaga cgcaggtgga
     3781 tgcacacctt ccctgtgggc ccctccggcg aggccatcca gatccaccac cagacccgcc
     3841 agaacatggc cgagctgcag ggctccggcc agcgcgaccc aacccactct agcgccgagc
     3901 tgctggagct ggcctatcac gaggccgccg gccggcacag caactccaga cagccaggcg
     3961 atggcatgag cttcctgaac ttttccggca cagaggagct gtccgtgggc ctgctgtcca
     4021 atagcggcgc cggcatgaac cctcgcaccc agaacaagga cagcctggag gattccgtgt
     4081 ccacctctcc cgatccaatc ctgacactgt ccgccccccc agtggtgcca ggcttttgct
     4141 gtaccgtggg cgtggattgg aagtccctga caaccccagc ctgtctgccc ctgaccaccg
     4201 attactttcc tgatagacag ggcctgcaga acgactacac cgagggctgc tatgatctgc
     4261 tgccagaggc cgacatcgac cggagggatg aggatggcgt gcagatgacc gcccagcagg
     4321 tgttcgagga gttcatctgc cagagactga tgcagggcta ccagatcatc gtgcagccta
     4381 agacccagaa gcctaatcca gccgtgccac ccccactgtc ttcctcccca ctgtactcca
     4441 gaggcctggt gtccagaaac cggccagagg aggaggacca gtactggctg agcatgggcc
     4501 ggacctttca caaggtgacc ctgaaggata agatgatcac agtgacaaga tatctgccaa
     4561 agtacccata cgagtccgcc cagatccact atacctattc tctgtgccct agccactccg
     4621 attccgagtt cgtgagctgc tgggtggagt ttagccacga gaggctggag gagtataagt
     4681 ggaactatct ggaccagtat atctgtagcg ccggctccga ggatttctcc ctgatcgagt
     4741 ccctgaagtt ttggcgcacc agatttctgc tgctgccagc ctgcgtgacc gccacaaagc
     4801 gcatcaccga gggcgaggcc cactgcgata tctatggcga tcggcctaga gccgacgagg
     4861 atgagtggca gctgctggat ggctttgtga gatttgtgga gggcctgaac agaatcaggc
     4921 ggcggcacag gtccgataga atgatgcgga agggcaccgc catgaagggc ctgcagatga
     4981 ccggccccat ctccacccac tccctggagt ctaccgcccc cccagtgggc aagaagggca
     5041 caagcgccct gtctgccctg ctggagatgg aggccagcca gaagtgcctg ggcgagcagc
     5101 aggccgccgt gcacggcggc aagagctccg cccagagcgc cgagtccagc tctgtggcca
     5161 tgacccccac atatatggac tccccacgca aggatggcgc cttctttatg gagtttgtgc
     5221 ggtctccaag aaccgcctcc tccgccttct atccacaggt gtccgtggat cagacagcca
     5281 ccccaatgct ggatggcaca agcctgggca tctgtacagg ccagagcatg gatagaggca
     5341 actctcagac cttcggcaac agccagaata tcggcgagca gggctattcc tccacaaact
     5401 cctccgactc ctcctcccag cagctggtgg cctccagcct gacaagctcc tccaccctga
     5461 cagagatcct ggaggccatg aagcacccat ctaccggcgt gcagctgctg tctgagcaga
     5521 agggcctgtc cccatactgc ttcatctccg ccgaggtggt gcactggctg gtgaatcacg
     5581 tggagggcat ccagacccag gccatggcca tcgacatcat gcagaagatg ctggaggagc
     5641 agctgatcac ccacgcctct ggcgaggcct ggagaacctt tatctacggc ttttacttct
     5701 acaagatcgt gacagataag gagcccgata gggtggccat gcagcagcct gccacaacct
     5761 ggcacaccgc cggcgtggat gattttgcca gctttcagcg gaagtggttc gaggtggcct
     5821 ttgtggccga ggagctggtg cacagcgaga tcccagcctt tctgctgcct tggctgccat
     5881 ctcgcccagc ctcttatgcc agcagacact ccagcttctc caggagcttc ggcggcagga
     5941 gccaggccgc cgccctgctg gccgccacag tgccagagca gagaaccgtg acactggacg
     6001 tggacgtgaa taacaggacc gacagactgg agtggtgttc ctgctactat cacggcaact
     6061 tctccctgaa tgccgccttc gagatcaagc tgcactggat ggccgtgacc gccgccgtgc
     6121 tgttcgagat ggtgcagggc tggcaccgga aggccacctc ttgtggcttt ctgctggtgc
     6181 ccgtgctgga gggcccattt gccctgccat cctacctgta cggcgatccc ctgagagccc
     6241 agctgtttat ccccctgaac atctcctgtc tgctgaagga gggctctgag cacctgtttg
     6301 acagcttcga gcctgagacc tactgggaca gaatgcacct gtttcaggag gccatcgccc
     6361 acaggttcgg ctttgtgcag gataagtact ccgccagcgc ctttaatttc cccgccgaga
     6421 ataagccaca gtacatccac gtgacaggca ccgtgttcct gcagctgcca tactccaagc
     6481 ggaagttttc cggccagcag cggaggcgga gaaattccac cagctctaca aaccagaaca
     6541 tgttctgtga ggagagagtg ggctacaatt gggcctacaa caccatgctg acaaagacat
     6601 ggagatcctc tgccacaggc gacgagaagt tcgccgatcg gctgctgaag gactttacag
     6661 acttctgcat caatcgggac aatcgcctgg tgaccttctg gacaagttgc ctggaaaaaa
     6721 tgcacgcctc cgccccctaa ctcgaggaat tcactcctca ggtgcaggct gcctatcaga
     6781 aggtggtggc tggtgtggcc aatgccctgg ctcacaaata ccactgagat ctttttccct
     6841 ctgccaaaaa ttatggggac atcatgaagc cccttgagca tctgacttct ggctaataaa
     6901 ggaaatttat tttcattgca atagtgtgtt ggaatttttt gtgtctctca ctcggaagga
     6961 catatgggag ggcaaatcat ttaaaacatc agaatgagta tttggtttag agtttggcaa
     7021 catatgccca tatgctggct gccatgaaca aaggttggct ataaagaggt catcagtata
     7081 tgaaacagcc ccctgctgtc cattccttat tccatagaaa agccttgact tgaggttaga
     7141 ttttttttat attttgtttt gtgttatttt tttctttaac atccctaaaa ttttccttac
     7201 atgttttact agccagattt ttcctcctct cctgactact cccagtcata gctgtccctc
     7261 ttctcttatg gagatccctc gacctgcagc ccaagcttgg cgtaatcatg gtcatagctg
     7321 tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata
     7381 aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca
     7441 ctgcccgctt tccagtcggg aaacctgtcg tgccagcgga tccgcatctc aattagtcag
     7501 caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc
     7561 attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg
     7621 cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa
     7681 agctaacttg tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt
     7741 cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt
     7801 atcttatcat gtctggatcc gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt
     7861 ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
     7921 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
     7981 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
     8041 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
     8101 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
     8161 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
     8221 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
     8281 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
     8341 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
     8401 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
     8461 ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct
     8521 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
     8581 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
     8641 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
     8701 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat
     8761 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac
     8821 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt
     8881 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt
     8941 gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag
     9001 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct
     9061 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt
     9121 gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc
     9181 tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt
     9241 agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg
     9301 gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg
     9361 actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct
     9421 tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc
     9481 attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt
     9541 tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt
     9601 tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg
     9661 aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta tcagggttat
     9721 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg
     9781 cgcacatttc cccgaaaagt gccacctggg tcgacattga ttattgacta gttattaata
     9841 gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg ttacataact
     9901 tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat
     9961 gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta
    10021 tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc
    10081 tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg
    10141 ggactttcct acttggcagt acatctacgt attagtcatc gctattacca tggtcgaggt
    10201 gagccccacg ttctgcttca ctctccccat ctcccccccc tccccacccc caattttgta
    10261 tttatttatt ttttaattat tttgtgcagc gatgggggcg ggggggggg
//
