LOCUS       sequence-327960-       10957 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of FLAG-tagged Tapasin L18K.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 10957)
  AUTHORS   McShan AC, Devlin CA, Morozov GI, Overall SA, Moschidi D, Akella N, 
            Procko E, Sgourakis NG
  TITLE     TAPBPR promotes antigen loading on MHC-I molecules using a peptide 
            trap.
  JOURNAL   Nat Commun. 2021 May 26;12(1):3174. doi: 10.1038/s41467-021-23225-6.
  PUBMED    34039964
REFERENCE   2  (bases 1 to 10957)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2021-05-26"; volume: "12(1):3174. doi"; pages: " 
            10.1038/s41467-021-23225-6"
COMMENT     Sequence Label: pCEP4-FLAG-Tapasin L18K
FEATURES             Location/Qualifiers
     source          1..10957
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     rep_origin      1327..3116
                     /label=oriP
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
     primer_bind     3676..3698
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     polyA_signal    3841..3975
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3878..3897)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3932..3951
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     CDS             complement(5294..5317)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     regulatory      5392..5401
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(5429..5453)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        complement(5450..5653)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(5479..5499)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        5654..6033
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     polyA_signal    6687..6735
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     6760..6779
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     CDS             complement(6777..7814)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPDRE
                     MGEAN"
     promoter        complement(7857..8002)
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     rep_origin      complement(8433..9021)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8513..8532)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9192..9983,9984..10052))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9815..9834
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10053..10157)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     10225..10243
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 gagcgggagg ggcaggagca ggaggggcag gagcaggagg aggggcagga gcaggaggnn
       61 nggcaggagc aggaggtgga ggccggggtc gaggaggcag tggaggccgg ggtcgaggag
      121 gtagtggagg ccggggtcga ggaggtagtg gaggccgccg gggtagagga cgtgaaagag
      181 ccaggggggg aagtcgtgaa agagccaggg ggagaggtcg tggacgtgga gaaaagaggc
      241 ccaggagtcc cagtagtcag tcatcatcat ccgggtctcc accgcgcagg ccccctccag
      301 gtagaaggcc atttttccac cctgtagggg aagccgatta ttttgaatac caccaagaag
      361 gtggcccaga tggtgagcct gacgtgcccc cgggagcgat agagcagggc cccgcagatg
      421 acccaggaga aggcccaagc actggacccc ggggtcaggg tgatggaggc aggcgcaaaa
      481 aaggagggtg gtttggaaag catcgtggtc aaggaggttc caacccgaaa tttgagaaca
      541 ttgcagaagg tttaagagct ctcctggcta ggagtcacgt agaaaggact accgacgaag
      601 gaacttgggt cgccggtgtg ttcgtatatg gaggtagtaa gacctccctt tacaacctaa
      661 ggcgaggaac tgcccttgct attccacaat gtcgtcttac accattgagt cgtctcccct
      721 ttggaatggc ccctggaccc ggcccacaac ctggcccgct aagggagtcc attgtctgtt
      781 atttcatggt ctttttacaa actcatatat ttgctgaggt tttgaaggat gcgattaagg
      841 accttgttat gacaaagccc gctcctacct gcaatatcag ggtgactgtg tgcagctttg
      901 acgatggagt agatttgcct ccctggtttc cacctatggt ggaaggggct gccgcggagg
      961 gtgatgacgg agatgacgga gatgaaggag gtgatggaga tgagggtgag gaagggcagg
     1021 agtgatgtaa cttgttagga gacgccctca atcgtattaa aagccgtgta ttcccccgca
     1081 ctaaagaata aatccccagt agacatcatg cgtgctgttg gtgtatttct ggccatctgt
     1141 cttgtcacca ttttcgtcct cccaacatgg ggcaattggg catacccatg ttgtcacgtc
     1201 actcagctcc gcgctcaaca ccttctcgcg ttggaaaaca ttagcgacat ttacctggtg
     1261 agcaatcaga catgcgacgg ctttagcctg gcctccttaa attcacctaa gaatgggagc
     1321 aaccagcagg aaaaggacaa gcagcgaaaa ttcacgcccc cttgggaggt ggcggcatat
     1381 gcaaaggata gcactcccac tctactactg ggtatcatat gctgactgta tatgcatgag
     1441 gatagcatat gctacccgga tacagattag gatagcatat actacccaga tatagattag
     1501 gatagcatat gctacccaga tatagattag gatagcctat gctacccaga tataaattag
     1561 gatagcatat actacccaga tatagattag gatagcatat gctacccaga tatagattag
     1621 gatagcctat gctacccaga tatagattag gatagcatat gctacccaga tatagattag
     1681 gatagcatat gctatccaga tatttgggta gtatatgcta cccagatata aattaggata
     1741 gcatatacta ccctaatctc tattaggata gcatatgcta cccggataca gattaggata
     1801 gcatatacta cccagatata gattaggata gcatatgcta cccagatata gattaggata
     1861 gcctatgcta cccagatata aattaggata gcatatacta cccagatata gattaggata
     1921 gcatatgcta cccagatata gattaggata gcctatgcta cccagatata gattaggata
     1981 gcatatgcta tccagatatt tgggtagtat atgctaccca tggcaacatt agcccaccgt
     2041 gctctcagcg acctcgtgaa tatgaggacc aacaaccctg tgcttggcgc tcaggcgcaa
     2101 gtgtgtgtaa tttgtcctcc agatcgcagc aatcgcgccc ctatcttggc ccgcccacct
     2161 acttatgcag gtattccccg gggtgccatt agtggttttg tgggcaagtg gtttgaccgc
     2221 agtggttagc ggggttacaa tcagccaagt tattacaccc ttattttaca gtccaaaacc
     2281 gcagggcggc gtgtgggggc tgacgcgtgc ccccactcca caatttcaaa aaaaagagtg
     2341 gccacttgtc tttgtttatg ggccccattg gcgtggagcc ccgtttaatt ttcgggggtg
     2401 ttagagacaa ccagtggagt ccgctgctgt cggcgtccac tctctttccc cttgttacaa
     2461 atagagtgta acaacatggt tcacctgtct tggtccctgc ctgggacaca tcttaataac
     2521 cccagtatca tattgcacta ggattatgtg ttgcccatag ccataaattc gtgtgagatg
     2581 gacatccagt ctttacggct tgtccccacc ccatggattt ctattgttaa agatattcag
     2641 aatgtttcat tcctacacta gtatttattg cccaaggggt ttgtgagggt tatattggtg
     2701 tcatagcaca atgccaccac tgaacccccc gtccaaattt tattctgggg gcgtcacctg
     2761 aaaccttgtt ttcgagcacc tcacatacac cttactgttc acaactcagc agttattcta
     2821 ttagctaaac gaaggagaat gaagaagcag gcgaagattc aggagagttc actgcccgct
     2881 ccttgatctt cagccactgc ccttgtgact aaaatggttc actaccctcg tggaatcctg
     2941 accccatgta aataaaaccg tgacagctca tggggtggga gatatcgctg ttccttagga
     3001 cccttttact aaccctaatt cgatagcata tgcttcccgt tgggtaacat atgctattga
     3061 attagggtta gtctggatag tatatactac tacccgggaa gcatatgcta cccgtttagg
     3121 gttaacaagg gggccttata aacactattg ctaatgccct cttgagggtc cgcttatcgg
     3181 tagctacaca ggcccctctg attgacgttg gtgtagcctc ccgtagtctt cctgggcccc
     3241 tgggaggtac atgtccccca gcattggtgt aagagcttca gccaagagtt acacataaag
     3301 gcaatgttgt gttgcagtcc acagactgca aagtctgctc caggatgaaa gccactcagt
     3361 gttggcaaat gtgcacatcc atttataagg atgtcaacta cagtcagaga acccctttgt
     3421 gtttggtccc cccccgtgtc acatgtggaa cagggcccag ttggcaagtt gtaccaacca
     3481 actgaaggga ttacatgcac tgccccgcga agaaggggca gagatgtcgt agtcaggttt
     3541 agttcgtccg gggcggggat cgatcctcta gagtcgacct catggctgcg ccccgacacc
     3601 cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac
     3661 aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac
     3721 gcgcgaggca gccggatcat aatcagccat accacatttg tagaggtttt acttgcttta
     3781 aaaaacctcc cacacctccc cctgaacctg aaacataaaa tgaatgcaat tgttgttgtt
     3841 aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca
     3901 aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct
     3961 tatcatgtct ggatccggcc ttgccggcct cgagttacta ttctgccttt ttcttgctgt
     4021 ctttacaggt gctgagataa actgctgccc atccgagtgc cttaaacaga cccagcaaca
     4081 ggaaagcgga caggaacaga ccaacactgt cctccaagct gggtccggac aacccagcga
     4141 cctcaagtgt tacctccgct gatcttcctg acgccggaag gctagggtgg tgaatgcgac
     4201 acgcataccg agctccatgc tgctcggtgg taactggcgg tggctgcaaa tgccctgaca
     4261 gtgatacaga tccgtctgag tggtgcctga gagcggagag ccatcgctgc ccttcagctt
     4321 tctgagatcg accacctggt cctcctctaa gttcccattc cacttcaagg ccaccactgg
     4381 ggtagaaatg ggataccaaa cagagcaatt caggtggagc ttcgccaggt gctgccctag
     4441 ccaaagttgc gggcatgaga ctcaccttag gcggtttata gacagcgagt tcaagtgtga
     4501 cctggccttg cagataaggc aaatggatgg tggccagata cgttccttct tgaaagggct
     4561 gtactgttgg cagccagaaa gtcccgtttc cagtccatgg tccccatggt tcgtcatcat
     4621 cccacgcagc aaaagctacg gctccctctt gtgctgccgg catctgcccg ttcagccctg
     4681 gggtagcagc caacagcaaa tggcccttcc ccaagtgctg tcgtctccat tcaaggccga
     4741 acggtggtgg tcctggtgca agagaggaag ccgcctctga ggttggtggc atgtacgcaa
     4801 agctaaggtc gaggagtgcg tcttgtccca atcgcaccct gggtgctggt gtatgcgtaa
     4861 ggaccgtaag gactaccgtc gccattgtta tcagtacggg ttcttgttgg ggttcaggtt
     4921 gtggcctgag cagtgatgac agtgaaagca ccggagagct tatggaaacc atcagccacg
     4981 ctccatcaag cgctcgtgga cagttttgtg ctggtgtcag acctgatgcc cactttgcac
     5041 tcgcaggcaa aggaacgaac cgactcatct cgcaatgcgg tgctggagca cctcttgggt
     5101 accgtcggaa agcagcttgg agtgctccag ctgggtcgtg tacagagagg tacaattcag
     5161 ggtccaagtc gggtcgtgga ggtggttctc ccggaccttg ccgcaacaga agtgcgcctg
     5221 gccgtttagc tttcccttta ccactagcgt cttcaacaaa ccaacactcg attacggcag
     5281 ggccggatcc tcccttgtca tcatcgtcct tgtagtcgga gcccgcccag gtctgggtca
     5341 gggccagggc ccccgagagt agcaggacga gggttcgggg cgccatgacg gccatggtgg
     5401 cgctagcagc tggtacccag cttctagaga tctgacggtt cactaaacga gctctgctta
     5461 tatagacctc ccaccgtaca cgcctaccgc ccatttgcgt caacggggcg gggttattac
     5521 gacattttgg aaagtcccgt tgattttggt gccaaaacaa actcccattg acgtcaatgg
     5581 ggtggagact tggaaatccc cgtgagtcaa accgctatcc acgcccattg gtgtactgcc
     5641 aaaaccgcat caccatggta atagcgatga ctaatacgta gatgtactgc caagtaggaa
     5701 agtcccgtaa ggtcatgtac tgggcataat gccaggcggg ccatttaccg tcattgacgt
     5761 caataggggg cggacttggc atatgataca cttgatgtac tgccaagtgg gcagtttacc
     5821 gtaaatactc cacccattga cgtcaatgga aagtccctat tggcgttact atgggaacat
     5881 acgtcattat tgacgtcaat gggcgggggt cgttgggcgg tcagccaggc gggccattta
     5941 ccgtaagtta tgtaacgcgg aactccatat atgggctatg aactaatgac cccgtaattg
     6001 attactatta ataactagtc aataatcaat gtcaacatgg cggtcatatt ggacatgagc
     6061 caatataaat gtacatatta tgatatagat acaacgtatg caatggccaa tagccaatat
     6121 tgatttatgc tatataacca atgactaata tggctaattg ccaatattga ttcaatgtat
     6181 agatcttcca tacctaccag ttctgcgcct gcagcaatgg caacaacgtt gcccggatct
     6241 gcgatgataa gctgtcaaac atgagaattg gtcgactagc ttggcacgcc agaaatccgc
     6301 gcggtggttt ttgggggtcg ggggtgtttg gcagccacag acgcccggtg ttcgtgtcgc
     6361 gccagtacat gcggtccatg cccaggccat ccaaaaacca tgggtctgtc tgctcagtcc
     6421 agtcgtggac ctgaccccac gcaacgccca aaataataac ccccacgaac cataaaccat
     6481 tccccatggg ggaccccgtc cctaacccac ggggccagtg gctatggcag ggcctgccgc
     6541 cccgacgttg gctgcgagcc ctgggccttc acccgaactt ggggggtggg gtggggaaaa
     6601 ggaagaaacg cgggcgtatt ggccccaatg gggtctcggt ggggtatcga cagagtgcca
     6661 gccctgggac cgaaccccgc gtttatgaac aaacgaccca acacccgtgc gttttattct
     6721 gtctttttat tgccgtcata gcgcgggttc cttccggtat tgtctccttc cgtgtttcag
     6781 ttagcctccc ccatctcccg atccggacga gtgctggggc gtcggtttcc actatcggcg
     6841 agtacttcta cacagccatc ggtccagacg gccgcgcttc tgcgggcgat ttgtgtacgc
     6901 ccgacagtcc cggctccgga tcggacgatt gcgtcgcatc gaccctgcgc ccaagctgca
     6961 tcatcgaaat tgccgtcaac caagctctga tagagttggt caagaccaat gcggagcata
     7021 tacgcccgga gccgcggcga tcctgcaagc tccggatgcc tccgctcgaa gtagcgcgtc
     7081 tgctgctcca tacaagccaa ccacggcctc cagaagaaga tgttggcgac ctcgtattgg
     7141 gaatccccga acatcgcctc gctccagtca atgaccgctg ttatgcggcc attgtccgtc
     7201 aggacattgt tggagccgaa atccgcgtgc acgaggtgcc ggacttcggg gcagtcctcg
     7261 gcccaaagca tcagctcatc gagagcctgc gcgacggacg cactgacggt gtcgtccatc
     7321 acagtttgcc agtgatacac atggggatca gcaatcgcgc atatgaaatc acgccatgta
     7381 gtgtattgac cgattccttg cggtccgaat gggccgaacc cgctcgtctg gctaagatcg
     7441 gccgcagcga tcgcatccat ggcctccgcg accggctgca gaacagcggg cagttcggtt
     7501 tcaggcaggt cttgcaacgt gacaccctgt gcacggcggg agatgcaata ggtcaggctc
     7561 tcgctgaatt ccccaatgtc aagcacttcc ggaatcggga gcgcggccga tgcaaagtgc
     7621 cgataaacat aacgatcttt gtagaaacca tcggcgcagc tatttacccg caggacatat
     7681 ccacgccctc ctacatcgaa gctgaaagca cgagattctt cgccctccga gagctgcatc
     7741 aggtcggaga cgctgtcgaa cttttcgatc agaaacttct cgacagacgt cgcggtgagt
     7801 tcaggctttt tcatatctca ttgccccccg ggatctgcgg cacgctgttg acgctgttaa
     7861 gcgggtcgct gcagggtcgc tcggtgttcg aggccacacg cgtcacctta atatgcgaag
     7921 tggacctcgg accgcgccgc cccgactgca tctgcgtgtt cgaattcgcc aatgacaaga
     7981 cgctgggcgg ggtttgtgtc atcatagaac taaagacatg caaatatatt tcttccgggg
     8041 acaccgccag caaacgcgag caacgggcca cggggatgaa gcagggcggc acctcgctaa
     8101 cggattcacc actccaagaa ttggagccaa tcaattcttg cggagaactg tgaatgcgca
     8161 aaccaaccct tggcagaaca tatccatcgc gtccgccatc tccagcagcc gcacgcggcg
     8221 catctcgggg ccgacgcgct gggctacgtc ttgctggcgt tcgcgacgcg aggctggatg
     8281 gccttcccca ttatgattct tctcgcttcc ggcggcatcg ggatgcccgc gttgcaggcc
     8341 atgctgtcca ggcaggtaga tgacgaccat cagggacagc ttcaaggcca gcaaaaggcc
     8401 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag
     8461 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac
     8521 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc
     8581 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt
     8641 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc
     8701 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga
     8761 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta
     8821 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aaggacagta
     8881 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga
     8941 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg
     9001 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag
     9061 tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc
     9121 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact
     9181 tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt
     9241 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta
     9301 ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta
     9361 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc
     9421 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat
     9481 agtttgcgca acgttgttgc cattgctgca ggcatcgtgg tgtcacgctc gtcgtttggt
     9541 atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg
     9601 tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca
     9661 gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta
     9721 agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg
     9781 cgaccgagtt gctcttgccc ggcgtcaaca cgggataata ccgcgccaca tagcagaact
     9841 ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg
     9901 ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt
     9961 actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga
    10021 ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc
    10081 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa
    10141 caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcta agaaaccatt
    10201 attatcatga cattaaccta taaaaatagg cgtatcacga ggccctttcg tcttcaagaa
    10261 ttctcatgtt tgacagctta tcatcgataa gctgatcctc acaggccgca cccagctttt
    10321 cttccgttgc cccagtagca tctctgtctg gtgaccttga agaggaagag gaggggtccc
    10381 gagaatcccc atccctaccg tccagcaaaa agggggacga ggaatttgag gcctggcttg
    10441 aggctcagga cgcaaatctt gaggatgttc agcgggagtt ttccgggctg cgagtaattg
    10501 gtgatgagga cgaggatggt tcggaggatg gggaattttc agacctggat ctgtctgaca
    10561 gcgaccatga aggggatgag ggtggggggg ctgttggagg gggcaggagt ctgcactccc
    10621 tgtattcact gagcgtcgtc taataaagat gtctattgat ctcttttagt gtgaatcatg
    10681 tctgacgagg ggccaggtac aggacctgga aatggcctag gagagaaggg agacacatct
    10741 ggaccagaag gctccggcgg cagtggacct caaagaagag ggggtgataa ccatggacga
    10801 ggacggggaa gaggacgagg acgaggaggc ggaagaccag gagccccggg cggctcagga
    10861 tcagggccaa gacatagaga tggtgtccgg agaccccaaa aacgtccaag ttgcattggc
    10921 tgcaaaggga cccacggtgg aacaggagca ggagcag
//
