LOCUS       sequence-327963-       10990 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of FLAG-tagged Tapasin L18G.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 10990)
  AUTHORS   McShan AC, Devlin CA, Morozov GI, Overall SA, Moschidi D, Akella N, 
            Procko E, Sgourakis NG
  TITLE     TAPBPR promotes antigen loading on MHC-I molecules using a peptide 
            trap.
  JOURNAL   Nat Commun. 2021 May 26;12(1):3174. doi: 10.1038/s41467-021-23225-6.
  PUBMED    34039964
REFERENCE   2  (bases 1 to 10990)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2021-05-26"; volume: "12(1):3174. doi"; pages: " 
            10.1038/s41467-021-23225-6"
COMMENT     Sequence Label: pCEP4-FLAG-Tapasin L18G
FEATURES             Location/Qualifiers
     source          1..10990
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     rep_origin      1366..3155
                     /label=oriP
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
     primer_bind     3715..3737
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     polyA_signal    3880..4014
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3917..3936)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3971..3990
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     CDS             complement(5333..5356)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     regulatory      5431..5440
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(5468..5492)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        complement(5489..5692)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(5518..5538)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        5693..6072
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     polyA_signal    6726..6774
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     6799..6818
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     CDS             complement(6816..7853)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPDRE
                     MGEAN"
     promoter        complement(7896..8041)
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     rep_origin      complement(8472..9060)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8552..8571)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9231..10022,10023..10091))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9854..9873
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10092..10196)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     10264..10282
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 gagcaggagc gggaggggca ggagcaggag gggcaggagc aggaggaggg gcaggagcag
       61 gaggaggggc aggagcagga ggaggggcag gagcaggagg ggcaggagca ggaggtggag
      121 gccggggtcg aggaggcagt ggaggccggg gtcgaggagg tagtggaggc cggggtcgag
      181 gaggtagtgg aggccgccgg ggtagaggac gtgaaagagc caggggggga agtcgtgaaa
      241 gagccagggg gagaggtcgt ggacgtggag aaaagaggcc caggagtccc agtagtcagt
      301 catcatcatc cgggtctcca ccgcgcaggc cccctccagg tagaaggcca tttttccacc
      361 ctgtagggga agccgattat tttgaatacc accaagaagg tggcccagat ggtgagcctg
      421 acgtgccccc gggagcgata gagcagggcc ccgcagatga cccaggagaa ggcccaagca
      481 ctggaccccg gggtcagggt gatggaggca ggcgcaaaaa aggagggtgg tttggaaagc
      541 atcgtggtca aggaggttcc aacccgaaat ttgagaacat tgcagaaggt ttaagagctc
      601 tcctggctag gagtcacgta gaaaggacta ccgacgaagg aacttgggtc gccggtgtgt
      661 tcgtatatgg aggtagtaag acctcccttt acaacctaag gcgaggaact gcccttgcta
      721 ttccacaatg tcgtcttaca ccattgagtc gtctcccctt tggaatggcc cctggacccg
      781 gcccacaacc tggcccgcta agggagtcca ttgtctgtta tttcatggtc tttttacaaa
      841 ctcatatatt tgctgaggtt ttgaaggatg cgattaagga ccttgttatg acaaagcccg
      901 ctcctacctg caatatcagg gtgactgtgt gcagctttga cgatggagta gatttgcctc
      961 cctggtttcc acctatggtg gaaggggctg ccgcggaggg tgatgacgga gatgacggag
     1021 atgaaggagg tgatggagat gagggtgagg aagggcagga gtgatgtaac ttgttaggag
     1081 acgccctcaa tcgtattaaa agccgtgtat tcccccgcac taaagaataa atccccagta
     1141 gacatcatgc gtgctgttgg tgtatttctg gccatctgtc ttgtcaccat tttcgtcctc
     1201 ccaacatggg gcaattgggc atacccatgt tgtcacgtca ctcagctccg cgctcaacac
     1261 cttctcgcgt tggaaaacat tagcgacatt tacctggtga gcaatcagac atgcgacggc
     1321 tttagcctgg cctccttaaa ttcacctaag aatgggagca accagcagga aaaggacaag
     1381 cagcgaaaat tcacgccccc ttgggaggtg gcggcatatg caaaggatag cactcccact
     1441 ctactactgg gtatcatatg ctgactgtat atgcatgagg atagcatatg ctacccggat
     1501 acagattagg atagcatata ctacccagat atagattagg atagcatatg ctacccagat
     1561 atagattagg atagcctatg ctacccagat ataaattagg atagcatata ctacccagat
     1621 atagattagg atagcatatg ctacccagat atagattagg atagcctatg ctacccagat
     1681 atagattagg atagcatatg ctacccagat atagattagg atagcatatg ctatccagat
     1741 atttgggtag tatatgctac ccagatataa attaggatag catatactac cctaatctct
     1801 attaggatag catatgctac ccggatacag attaggatag catatactac ccagatatag
     1861 attaggatag catatgctac ccagatatag attaggatag cctatgctac ccagatataa
     1921 attaggatag catatactac ccagatatag attaggatag catatgctac ccagatatag
     1981 attaggatag cctatgctac ccagatatag attaggatag catatgctat ccagatattt
     2041 gggtagtata tgctacccat ggcaacatta gcccaccgtg ctctcagcga cctcgtgaat
     2101 atgaggacca acaaccctgt gcttggcgct caggcgcaag tgtgtgtaat ttgtcctcca
     2161 gatcgcagca atcgcgcccc tatcttggcc cgcccaccta cttatgcagg tattccccgg
     2221 ggtgccatta gtggttttgt gggcaagtgg tttgaccgca gtggttagcg gggttacaat
     2281 cagccaagtt attacaccct tattttacag tccaaaaccg cagggcggcg tgtgggggct
     2341 gacgcgtgcc cccactccac aatttcaaaa aaaagagtgg ccacttgtct ttgtttatgg
     2401 gccccattgg cgtggagccc cgtttaattt tcgggggtgt tagagacaac cagtggagtc
     2461 cgctgctgtc ggcgtccact ctctttcccc ttgttacaaa tagagtgtaa caacatggtt
     2521 cacctgtctt ggtccctgcc tgggacacat cttaataacc ccagtatcat attgcactag
     2581 gattatgtgt tgcccatagc cataaattcg tgtgagatgg acatccagtc tttacggctt
     2641 gtccccaccc catggatttc tattgttaaa gatattcaga atgtttcatt cctacactag
     2701 tatttattgc ccaaggggtt tgtgagggtt atattggtgt catagcacaa tgccaccact
     2761 gaaccccccg tccaaatttt attctggggg cgtcacctga aaccttgttt tcgagcacct
     2821 cacatacacc ttactgttca caactcagca gttattctat tagctaaacg aaggagaatg
     2881 aagaagcagg cgaagattca ggagagttca ctgcccgctc cttgatcttc agccactgcc
     2941 cttgtgacta aaatggttca ctaccctcgt ggaatcctga ccccatgtaa ataaaaccgt
     3001 gacagctcat ggggtgggag atatcgctgt tccttaggac ccttttacta accctaattc
     3061 gatagcatat gcttcccgtt gggtaacata tgctattgaa ttagggttag tctggatagt
     3121 atatactact acccgggaag catatgctac ccgtttaggg ttaacaaggg ggccttataa
     3181 acactattgc taatgccctc ttgagggtcc gcttatcggt agctacacag gcccctctga
     3241 ttgacgttgg tgtagcctcc cgtagtcttc ctgggcccct gggaggtaca tgtcccccag
     3301 cattggtgta agagcttcag ccaagagtta cacataaagg caatgttgtg ttgcagtcca
     3361 cagactgcaa agtctgctcc aggatgaaag ccactcagtg ttggcaaatg tgcacatcca
     3421 tttataagga tgtcaactac agtcagagaa cccctttgtg tttggtcccc ccccgtgtca
     3481 catgtggaac agggcccagt tggcaagttg taccaaccaa ctgaagggat tacatgcact
     3541 gccccgcgaa gaaggggcag agatgtcgta gtcaggttta gttcgtccgg ggcggggatc
     3601 gatcctctag agtcgacctc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc
     3661 cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga
     3721 gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ccggatcata
     3781 atcagccata ccacatttgt agaggtttta cttgctttaa aaaacctccc acacctcccc
     3841 ctgaacctga aacataaaat gaatgcaatt gttgttgtta acttgtttat tgcagcttat
     3901 aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg
     3961 cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg gatccggcct
     4021 tgccggcctc gagttactat tctgcctttt tcttgctgtc tttacaggtg ctgagataaa
     4081 ctgctgccca tccgagtgcc ttaaacagac ccagcaacag gaaagcggac aggaacagac
     4141 caacactgtc ctccaagctg ggtccggaca acccagcgac ctcaagtgtt acctccgctg
     4201 atcttcctga cgccggaagg ctagggtggt gaatgcgaca cgcataccga gctccatgct
     4261 gctcggtggt aactggcggt ggctgcaaat gccctgacag tgatacagat ccgtctgagt
     4321 ggtgcctgag agcggagagc catcgctgcc cttcagcttt ctgagatcga ccacctggtc
     4381 ctcctctaag ttcccattcc acttcaaggc caccactggg gtagaaatgg gataccaaac
     4441 agagcaattc aggtggagct tcgccaggtg ctgccctagc caaagttgcg ggcatgagac
     4501 tcaccttagg cggtttatag acagcgagtt caagtgtgac ctggccttgc agataaggca
     4561 aatggatggt ggccagatac gttccttctt gaaagggctg tactgttggc agccagaaag
     4621 tcccgtttcc agtccatggt ccccatggtt cgtcatcatc ccacgcagca aaagctacgg
     4681 ctccctcttg tgctgccggc atctgcccgt tcagccctgg ggtagcagcc aacagcaaat
     4741 ggcccttccc caagtgctgt cgtctccatt caaggccgaa cggtggtggt cctggtgcaa
     4801 gagaggaagc cgcctctgag gttggtggca tgtacgcaaa gctaaggtcg aggagtgcgt
     4861 cttgtcccaa tcgcaccctg ggtgctggtg tatgcgtaag gaccgtaagg actaccgtcg
     4921 ccattgttat cagtacgggt tcttgttggg gttcaggttg tggcctgagc agtgatgaca
     4981 gtgaaagcac cggagagctt atggaaacca tcagccacgc tccatcaagc gctcgtggac
     5041 agttttgtgc tggtgtcaga cctgatgccc actttgcact cgcaggcaaa ggaacgaacc
     5101 gactcatctc gcaatgcggt gctggagcac ctcttgggta ccgtcggaaa gcagcttgga
     5161 gtgctccagc tgggtcgtgt acagagaggt acaattcagg gtccaagtcg ggtcgtggag
     5221 gtggttctcc cggaccttgc cgcaacagaa gtgcgcctgg ccgtttagcg ccccctttac
     5281 cactagcgtc ttcaacaaac caacactcga ttacggcagg gccggatcct cccttgtcat
     5341 catcgtcctt gtagtcggag cccgcccagg tctgggtcag ggccagggcc cccgagagta
     5401 gcaggacgag ggttcggggc gccatgacgg ccatggtggc gctagcagct ggtacccagc
     5461 ttctagagat ctgacggttc actaaacgag ctctgcttat atagacctcc caccgtacac
     5521 gcctaccgcc catttgcgtc aacggggcgg ggttattacg acattttgga aagtcccgtt
     5581 gattttggtg ccaaaacaaa ctcccattga cgtcaatggg gtggagactt ggaaatcccc
     5641 gtgagtcaaa ccgctatcca cgcccattgg tgtactgcca aaaccgcatc accatggtaa
     5701 tagcgatgac taatacgtag atgtactgcc aagtaggaaa gtcccgtaag gtcatgtact
     5761 gggcataatg ccaggcgggc catttaccgt cattgacgtc aatagggggc ggacttggca
     5821 tatgatacac ttgatgtact gccaagtggg cagtttaccg taaatactcc acccattgac
     5881 gtcaatggaa agtccctatt ggcgttacta tgggaacata cgtcattatt gacgtcaatg
     5941 ggcgggggtc gttgggcggt cagccaggcg ggccatttac cgtaagttat gtaacgcgga
     6001 actccatata tgggctatga actaatgacc ccgtaattga ttactattaa taactagtca
     6061 ataatcaatg tcaacatggc ggtcatattg gacatgagcc aatataaatg tacatattat
     6121 gatatagata caacgtatgc aatggccaat agccaatatt gatttatgct atataaccaa
     6181 tgactaatat ggctaattgc caatattgat tcaatgtata gatcttccat acctaccagt
     6241 tctgcgcctg cagcaatggc aacaacgttg cccggatctg cgatgataag ctgtcaaaca
     6301 tgagaattgg tcgactagct tggcacgcca gaaatccgcg cggtggtttt tgggggtcgg
     6361 gggtgtttgg cagccacaga cgcccggtgt tcgtgtcgcg ccagtacatg cggtccatgc
     6421 ccaggccatc caaaaaccat gggtctgtct gctcagtcca gtcgtggacc tgaccccacg
     6481 caacgcccaa aataataacc cccacgaacc ataaaccatt ccccatgggg gaccccgtcc
     6541 ctaacccacg gggccagtgg ctatggcagg gcctgccgcc ccgacgttgg ctgcgagccc
     6601 tgggccttca cccgaacttg gggggtgggg tggggaaaag gaagaaacgc gggcgtattg
     6661 gccccaatgg ggtctcggtg gggtatcgac agagtgccag ccctgggacc gaaccccgcg
     6721 tttatgaaca aacgacccaa cacccgtgcg ttttattctg tctttttatt gccgtcatag
     6781 cgcgggttcc ttccggtatt gtctccttcc gtgtttcagt tagcctcccc catctcccga
     6841 tccggacgag tgctggggcg tcggtttcca ctatcggcga gtacttctac acagccatcg
     6901 gtccagacgg ccgcgcttct gcgggcgatt tgtgtacgcc cgacagtccc ggctccggat
     6961 cggacgattg cgtcgcatcg accctgcgcc caagctgcat catcgaaatt gccgtcaacc
     7021 aagctctgat agagttggtc aagaccaatg cggagcatat acgcccggag ccgcggcgat
     7081 cctgcaagct ccggatgcct ccgctcgaag tagcgcgtct gctgctccat acaagccaac
     7141 cacggcctcc agaagaagat gttggcgacc tcgtattggg aatccccgaa catcgcctcg
     7201 ctccagtcaa tgaccgctgt tatgcggcca ttgtccgtca ggacattgtt ggagccgaaa
     7261 tccgcgtgca cgaggtgccg gacttcgggg cagtcctcgg cccaaagcat cagctcatcg
     7321 agagcctgcg cgacggacgc actgacggtg tcgtccatca cagtttgcca gtgatacaca
     7381 tggggatcag caatcgcgca tatgaaatca cgccatgtag tgtattgacc gattccttgc
     7441 ggtccgaatg ggccgaaccc gctcgtctgg ctaagatcgg ccgcagcgat cgcatccatg
     7501 gcctccgcga ccggctgcag aacagcgggc agttcggttt caggcaggtc ttgcaacgtg
     7561 acaccctgtg cacggcggga gatgcaatag gtcaggctct cgctgaattc cccaatgtca
     7621 agcacttccg gaatcgggag cgcggccgat gcaaagtgcc gataaacata acgatctttg
     7681 tagaaaccat cggcgcagct atttacccgc aggacatatc cacgccctcc tacatcgaag
     7741 ctgaaagcac gagattcttc gccctccgag agctgcatca ggtcggagac gctgtcgaac
     7801 ttttcgatca gaaacttctc gacagacgtc gcggtgagtt caggcttttt catatctcat
     7861 tgccccccgg gatctgcggc acgctgttga cgctgttaag cgggtcgctg cagggtcgct
     7921 cggtgttcga ggccacacgc gtcaccttaa tatgcgaagt ggacctcgga ccgcgccgcc
     7981 ccgactgcat ctgcgtgttc gaattcgcca atgacaagac gctgggcggg gtttgtgtca
     8041 tcatagaact aaagacatgc aaatatattt cttccgggga caccgccagc aaacgcgagc
     8101 aacgggccac ggggatgaag cagggcggca cctcgctaac ggattcacca ctccaagaat
     8161 tggagccaat caattcttgc ggagaactgt gaatgcgcaa accaaccctt ggcagaacat
     8221 atccatcgcg tccgccatct ccagcagccg cacgcggcgc atctcggggc cgacgcgctg
     8281 ggctacgtct tgctggcgtt cgcgacgcga ggctggatgg ccttccccat tatgattctt
     8341 ctcgcttccg gcggcatcgg gatgcccgcg ttgcaggcca tgctgtccag gcaggtagat
     8401 gacgaccatc agggacagct tcaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
     8461 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
     8521 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
     8581 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
     8641 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag
     8701 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
     8761 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
     8821 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
     8881 aagtggtggc ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg
     8941 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
     9001 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
     9061 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
     9121 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
     9181 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
     9241 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
     9301 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
     9361 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
     9421 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
     9481 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
     9541 attgctgcag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
     9601 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
     9661 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
     9721 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
     9781 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
     9841 gcgtcaacac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
     9901 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
     9961 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
    10021 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
    10081 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
    10141 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
    10201 tttccccgaa aagtgccacc tgacgtctaa gaaaccatta ttatcatgac attaacctat
    10261 aaaaataggc gtatcacgag gccctttcgt cttcaagaat tctcatgttt gacagcttat
    10321 catcgataag ctgatcctca caggccgcac ccagcttttc ttccgttgcc ccagtagcat
    10381 ctctgtctgg tgaccttgaa gaggaagagg aggggtcccg agaatcccca tccctaccgt
    10441 ccagcaaaaa gggggacgag gaatttgagg cctggcttga ggctcaggac gcaaatcttg
    10501 aggatgttca gcgggagttt tccgggctgc gagtaattgg tgatgaggac gaggatggtt
    10561 cggaggatgg ggaattttca gacctggatc tgtctgacag cgaccatgaa ggggatgagg
    10621 gtgggggggc tgttggaggg ggcaggagtc tgcactccct gtattcactg agcgtcgtct
    10681 aataaagatg tctattgatc tcttttagtg tgaatcatgt ctgacgaggg gccaggtaca
    10741 ggacctggaa atggcctagg agagaaggga gacacatctg gaccagaagg ctccggcggc
    10801 agtggacctc aaagaagagg gggtgataac catggacgag gacggggaag aggacgagga
    10861 cgaggaggcg gaagaccagg agccccgggc ggctcaggat cagggccaag acatagagat
    10921 ggtgtccgga gaccccaaaa acgtccaagt tgcattggct gcaaagggac ccacggtgga
    10981 acaggagcag
//
