LOCUS       sequence-327964-       10924 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of FLAG-tagged Tapasin L18E.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 10924)
  AUTHORS   McShan AC, Devlin CA, Morozov GI, Overall SA, Moschidi D, Akella N, 
            Procko E, Sgourakis NG
  TITLE     TAPBPR promotes antigen loading on MHC-I molecules using a peptide 
            trap.
  JOURNAL   Nat Commun. 2021 May 26;12(1):3174. doi: 10.1038/s41467-021-23225-6.
  PUBMED    34039964
REFERENCE   2  (bases 1 to 10924)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2021-05-26"; volume: "12(1):3174. doi"; pages: " 
            10.1038/s41467-021-23225-6"
COMMENT     Sequence Label: pCEP4-FLAG-Tapasin L18E
FEATURES             Location/Qualifiers
     source          1..10924
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     581..605
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      633..642
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             717..740
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     polyA_signal    2059..2193
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2083..2102)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2137..2156
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(2336..2358)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(6715..6733)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6801..6905
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6906..6974,6975..7766)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7124..7143)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7937..8525
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8426..8445
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        8956..9101
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     CDS             9144..10181
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPDRE
                     MGEAN"
     primer_bind     complement(10179..10198)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    10223..10271
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt ccgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttacggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta caccaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaataa ccccgccccg ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctcgttta gtgaaccgtc
      601 agatctctag aagctgggta ccagctgcta gcgccaccat ggccgtcatg gcgccccgaa
      661 ccctcgtcct gctactctcg ggggccctgg ccctgaccca gacctgggcg ggctccgact
      721 acaaggacga tgatgacaag ggaggatccg gccctgccgt aatcgagtgt tggtttgttg
      781 aagacgctag tggtaaaggg gaagctaaac ggccaggcgc acttctgttg cggcaaggtc
      841 cgggagaacc acctccacga cccgacttgg accctgaatt gtacctctct gtacacgacc
      901 cagctggagc actccaagct gctttccgac ggtacccaag aggtgctcca gcaccgcatt
      961 gcgagatgag tcggttcgtt cctttgcctg cgagtgcaaa gtgggcatca ggtctgacac
     1021 cagcacaaaa ctgtccacga gcgcttgatg gagcgtggct gatggtttcc ataagctctc
     1081 cggtgctttc actgtcatca ctgctcaggc cacaacctga accccaacaa gaacccgtac
     1141 tgataacaat ggcgacggta gtccttacgg tccttacgca tacaccagca cccagggtgc
     1201 gattgggaca agacgcactc ctcgacctta gctttgcgta catgccacca acctcagagg
     1261 cggcttcctc tcttgcacca ggaccaccac cgttcggcct tgaatggaga cgacagcact
     1321 tggggaaggg ccatttgctg ttggctgcta ccccagggct gaacgggcag atgccggcag
     1381 cacaagaggg agccgtagct tttgctgcgt gggatgatga cgaaccatgg ggaccatgga
     1441 ctggaaacgg gactttctgg ctgccaacag tacagccctt tcaagaagga acgtatctgg
     1501 ccaccatcca tttgccttat ctgcaaggcc aggtcacact tgaactcgct gtctataaac
     1561 cgcctaaggt gagtctcatg cccgcaactt tggctagggc agcacctggc gaagctccac
     1621 ctgaattgct ctgtttggta tcccatttct accccagtgg tggccttgaa gtggaatggg
     1681 aacttagagg aggaccaggt ggtcgatctc agaaagctga agggcagcga tggctctccg
     1741 ctctcaggca ccactcagac ggatctgtat cactgtcagg gcatttgcag ccaccgccag
     1801 ttaccaccga gcagcatgga gctcggtatg cgtgtcgcat tcaccaccct agccttccgg
     1861 cgtcaggaag atcagcggag gtaacacttg aggtcgctgg gttgtccgga cccagcttgg
     1921 aggacagtgt tggtctgttc ctgtccgctt tcctgttgct gggtctgttt aaggcactcg
     1981 gatgggcagc agtttatctc agcacctgta aagacagcaa gaaaaaggca gaatagtaac
     2041 tcgaggccgg caaggccgga tccagacatg ataagataca ttgatgagtt tggacaaacc
     2101 acaactagaa tgcagtgaaa aaaatgcttt atttgtgaaa tttgtgatgc tattgcttta
     2161 tttgtaacca ttataagctg caataaacaa gttaacaaca acaattgcat tcattttatg
     2221 tttcaggttc agggggaggt gtgggaggtt ttttaaagca agtaaaacct ctacaaatgt
     2281 ggtatggctg attatgatcc ggctgcctcg cgcgtttcgg tgatgacggt gaaaacctct
     2341 gacacatgca gctcccggag acggtcacag cttgtctgta agcggatgcc gggagcagac
     2401 aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg gggcgcagcc atgaggtcga
     2461 ctctagagga tcgatccccg ccccggacga actaaacctg actacgacat ctctgcccct
     2521 tcttcgcggg gcagtgcatg taatcccttc agttggttgg tacaacttgc caactgggcc
     2581 ctgttccaca tgtgacacgg ggggggacca aacacaaagg ggttctctga ctgtagttga
     2641 catccttata aatggatgtg cacatttgcc aacactgagt ggctttcatc ctggagcaga
     2701 ctttgcagtc tgtggactgc aacacaacat tgcctttatg tgtaactctt ggctgaagct
     2761 cttacaccaa tgctggggga catgtacctc ccaggggccc aggaagacta cgggaggcta
     2821 caccaacgtc aatcagaggg gcctgtgtag ctaccgataa gcggaccctc aagagggcat
     2881 tagcaatagt gtttataagg cccccttgtt aaccctaaac gggtagcata tgcttcccgg
     2941 gtagtagtat atactatcca gactaaccct aattcaatag catatgttac ccaacgggaa
     3001 gcatatgcta tcgaattagg gttagtaaaa gggtcctaag gaacagcgat atctcccacc
     3061 ccatgagctg tcacggtttt atttacatgg ggtcaggatt ccacgagggt agtgaaccat
     3121 tttagtcaca agggcagtgg ctgaagatca aggagcgggc agtgaactct cctgaatctt
     3181 cgcctgcttc ttcattctcc ttcgtttagc taatagaata actgctgagt tgtgaacagt
     3241 aaggtgtatg tgaggtgctc gaaaacaagg tttcaggtga cgcccccaga ataaaatttg
     3301 gacggggggt tcagtggtgg cattgtgcta tgacaccaat ataaccctca caaacccctt
     3361 gggcaataaa tactagtgta ggaatgaaac attctgaata tctttaacaa tagaaatcca
     3421 tggggtgggg acaagccgta aagactggat gtccatctca cacgaattta tggctatggg
     3481 caacacataa tcctagtgca atatgatact ggggttatta agatgtgtcc caggcaggga
     3541 ccaagacagg tgaaccatgt tgttacactc tatttgtaac aaggggaaag agagtggacg
     3601 ccgacagcag cggactccac tggttgtctc taacaccccc gaaaattaaa cggggctcca
     3661 cgccaatggg gcccataaac aaagacaagt ggccactctt ttttttgaaa ttgtggagtg
     3721 ggggcacgcg tcagccccca cacgccgccc tgcggttttg gactgtaaaa taagggtgta
     3781 ataacttggc tgattgtaac cccgctaacc actgcggtca aaccacttgc ccacaaaacc
     3841 actaatggca ccccggggaa tacctgcata agtaggtggg cgggccaaga taggggcgcg
     3901 attgctgcga tctggaggac aaattacaca cacttgcgcc tgagcgccaa gcacagggtt
     3961 gttggtcctc atattcacga ggtcgctgag agcacggtgg gctaatgttg ccatgggtag
     4021 catatactac ccaaatatct ggatagcata tgctatccta atctatatct gggtagcata
     4081 ggctatccta atctatatct gggtagcata tgctatccta atctatatct gggtagtata
     4141 tgctatccta atttatatct gggtagcata ggctatccta atctatatct gggtagcata
     4201 tgctatccta atctatatct gggtagtata tgctatccta atmkrkatym gggtagyata
     4261 tgctatccta atttatatct gggtagcata tactacccaa atatctggat agcatatgct
     4321 atcctaatct atatctgggt agcatatgct atcctaatct atatctgggt agcataggct
     4381 atcctaatct atatctgggt agcatatgct atcctaatct atatctgggt agtatatgct
     4441 atcctaattt atatctgggt agcataggct atcctaatct atatctgggt agcatatgct
     4501 atcctaatct atatctgggt agtatatgct atcctaatct gtatccgggt agcatatgct
     4561 atcctcatgc atatacagtc agcatatgat acccagtagt agagtgggag tgctatcctt
     4621 tgcatatgcc gccacctccc aagggggcgt gaattttcgc tgcttgtcct tttcctgctg
     4681 gttgctccca ttcttaggtg aatttaagga ggccaggcta aagccgtcgc atgtctgatt
     4741 gctcaccagg taaatgtcgc taatgttttc caacgcgaga aggtgttgag cgcggagctg
     4801 agtgacgtga caacatgggt atgcccaatt gccccatgtt gggaggacga aaatggtgac
     4861 aagacagatg gccagaaata caccaacagc acgcatgatg tctactgggg atttattctt
     4921 tagtgcgggg gaatacacgg cttttaatac gattgagggc gtctcctaac aagttacatc
     4981 actcctgccc ttcctcaccc tcatctccat cacctccttc atctccgtca tctccgtcat
     5041 caccctccgc ggcagcccct tccaccatag gtggaaacca gggaggcaaa tctactccat
     5101 cgtcaaagct gcacacagtc accctgatat tgcaggtagg agcgggcttt gtcataacaa
     5161 ggtccttaat cgcatccttc aaaacctcag caaatatatg agtttgtaaa aagaccatga
     5221 aataacagac aatggactcc cttagcgggc caggttgtgg gccgggtcca ggggccattc
     5281 caaaggggag acgactcaat ggtgtaagac gacattgtgg aatagcaagg gcagttcctc
     5341 gccttaggtt gtaaagggag gtcttactac ctccatatac gaacacaccg gcgacccaag
     5401 ttccttcgtc ggtagtcctt tctacgtgac tcctagccag gagagctctt aaaccttctg
     5461 caatgttctc aaatttcggg ttggaacctc cttgaccacg atgctttcca aaccaccctc
     5521 cttttttgcg cctgcctcca tcaccctgac cccggggtcc agtgcttggg ccttctcctg
     5581 ggtcatctgc ggggccctgc tctatcgctc ccgggggcac gtcaggctca ccatctgggc
     5641 caccttcttg gtggtattca aaataatcgg cttcccctac agggtggaaa aatggccttc
     5701 tacctggagg gggcctgcgc ggtggagacc cggatgatga tgactgacta ctgggactcc
     5761 tgggcctctt ttctccacgt ccacgacctc tccccctggc tctttcacga cttccccccc
     5821 tggctctttc acgtcctcta ccccggcggc ctccactacc tcctcgaccc cggcctccac
     5881 tacctcctcg accccggcct ccactgcctc ctcgaccccg gcctccacct cctgctcctg
     5941 cccctcctgc tcctgcccct cctcctgctc ctgcccctcc tgctcctgcc cctcccgctc
     6001 ctgctcctgc tcctgttcca ccgtgggtcc ctttgcagcc aatgcaactt ggacgttttt
     6061 ggggtctccg gacaccatct ctatgtcttg gccctgatcc tgagccgccc ggggctcctg
     6121 gtcttccgcc tcctcgtcct cgtcctcttc cccgtcctcg tccatggtta tcaccccctc
     6181 ttctttgagg tccactgccg ccggagcctt ctggtccaga tgtgtctccc ttctctccta
     6241 ggccatttcc aggtcctgta cctggcccct cgtcagacat gattcacact aaaagagatc
     6301 aatagacatc tttattagac gacgctcagt gaatacaggg agtgcagact cctgccccct
     6361 ccaacagccc ccccaccctc atccccttca tggtcgctgt cagacagatc caggtctgaa
     6421 aattccccat cctccgaacc atcctcgtcc tcatcaccaa ttactcgcag cccggaaaac
     6481 tcccgctgaa catcctcaag atttgcgtcc tgagcctcaa gccaggcctc aaattcctcg
     6541 tccccctttt tgctggacgg tagggatggg gattctcggg acccctcctc ttcctcttca
     6601 aggtcaccag acagagatgc tactggggca acggaagaaa agctgggtgc ggcctgtgag
     6661 gatcagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga aagggcctcg
     6721 tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg
     6781 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa
     6841 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga
     6901 agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc
     6961 ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg
     7021 gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc
     7081 gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat
     7141 tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg
     7201 acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag
     7261 aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa
     7321 cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc
     7381 gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca
     7441 cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc
     7501 tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc
     7561 tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg
     7621 ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta
     7681 tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag
     7741 gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga
     7801 ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc
     7861 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
     7921 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
     7981 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
     8041 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt
     8101 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
     8161 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
     8221 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
     8281 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
     8341 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
     8401 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
     8461 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
     8521 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttgaagct
     8581 gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga
     8641 tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg
     8701 ccagcaagac gtagcccagc gcgtcggccc cgagatgcgc cgcgtgcggc tgctggagat
     8761 ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa
     8821 gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccctgcttc
     8881 atccccgtgg cccgttgctc gcgtttgctg gcggtgtccc cggaagaaat atatttgcat
     8941 gtctttagtt ctatgatgac acaaaccccg cccagcgtct tgtcattggc gaattcgaac
     9001 acgcagatgc agtcggggcg gcgcggtccg aggtccactt cgcatattaa ggtgacgcgt
     9061 gtggcctcga acaccgagcg accctgcagc gacccgctta acagcgtcaa cagcgtgccg
     9121 cagatcccgg ggggcaatga gatatgaaaa agcctgaact caccgcgacg tctgtcgaga
     9181 agtttctgat cgaaaagttc gacagcgtct ccgacctgat gcagctctcg gagggcgaag
     9241 aatctcgtgc tttcagcttc gatgtaggag ggcgtggata tgtcctgcgg gtaaatagct
     9301 gcgccgatgg tttctacaaa gatcgttatg tttatcggca ctttgcatcg gccgcgctcc
     9361 cgattccgga agtgcttgac attggggaat tcagcgagag cctgacctat tgcatctccc
     9421 gccgtgcaca gggtgtcacg ttgcaagacc tgcctgaaac cgaactgccc gctgttctgc
     9481 agccggtcgc ggaggccatg gatgcgatcg ctgcggccga tcttagccag acgagcgggt
     9541 tcggcccatt cggaccgcaa ggaatcggtc aatacactac atggcgtgat ttcatatgcg
     9601 cgattgctga tccccatgtg tatcactggc aaactgtgat ggacgacacc gtcagtgcgt
     9661 ccgtcgcgca ggctctcgat gagctgatgc tttgggccga ggactgcccc gaagtccggc
     9721 acctcgtgca cgcggatttc ggctccaaca atgtcctgac ggacaatggc cgcataacag
     9781 cggtcattga ctggagcgag gcgatgttcg gggattccca atacgaggtc gccaacatct
     9841 tcttctggag gccgtggttg gcttgtatgg agcagcagac gcgctacttc gagcggaggc
     9901 atccggagct tgcaggatcg ccgcggctcc gggcgtatat gctccgcatt ggtcttgacc
     9961 aactctatca gagcttggtt gacggcaatt tcgatgatgc agcttgggcg cagggtcgat
    10021 gcgacgcaat cgtccgatcc ggagccggga ctgtcgggcg tacacaaatc gcccgcagaa
    10081 gcgcggccgt ctggaccgat ggctgtgtag aagtactcgc cgatagtgga aaccgacgcc
    10141 ccagcactcg tccggatcgg gagatggggg aggctaactg aaacacggaa ggagacaata
    10201 ccggaaggaa cccgcgctat gacggcaata aaaagacaga ataaaacgca cgggtgttgg
    10261 gtcgtttgtt cataaacgcg gggttcggtc ccagggctgg cactctgtcg ataccccacc
    10321 gagaccccat tggggccaat acgcccgcgt ttcttccttt tccccacccc accccccaag
    10381 ttcgggtgaa ggcccagggc tcgcagccaa cgtcggggcg gcaggccctg ccatagccac
    10441 tggccccgtg ggttagggac ggggtccccc atggggaatg gtttatggtt cgtgggggtt
    10501 attattttgg gcgttgcgtg gggtcaggtc cacgactgga ctgagcagac agacccatgg
    10561 tttttggatg gcctgggcat ggaccgcatg tactggcgcg acacgaacac cgggcgtctg
    10621 tggctgccaa acacccccga cccccaaaaa ccaccgcgcg gatttctggc gtgccaagct
    10681 agtcgaccaa ttctcatgtt tgacagctta tcatcgcaga tccgggcaac gttgttgcca
    10741 ttgctgcagg cgcagaactg gtaggtatgg aagatctata cattgaatca atattggcaa
    10801 ttagccatat tagtcattgg ttatatagca taaatcaata ttggctattg gccattgcat
    10861 acgttgtatc tatatcataa tatgtacatt tatattggct catgtccaat atgaccgcca
    10921 tgtt
//
