LOCUS       sequence-327967-       10646 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of FLAG-tagged Tapasin G15L.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 10646)
  AUTHORS   McShan AC, Devlin CA, Morozov GI, Overall SA, Moschidi D, Akella N, 
            Procko E, Sgourakis NG
  TITLE     TAPBPR promotes antigen loading on MHC-I molecules using a peptide 
            trap.
  JOURNAL   Nat Commun. 2021 May 26;12(1):3174. doi: 10.1038/s41467-021-23225-6.
  PUBMED    34039964
REFERENCE   2  (bases 1 to 10646)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2021-05-26"; volume: "12(1):3174. doi"; pages: " 
            10.1038/s41467-021-23225-6"
COMMENT     Sequence Label: pCEP4-FLAG-Tapasin G15L
FEATURES             Location/Qualifiers
     source          1..10646
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     581..605
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      633..642
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             717..740
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     polyA_signal    2059..2193
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2083..2102)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2137..2156
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(2336..2358)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(6437..6455)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6523..6627
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6628..6696,6697..7488)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6846..6865)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7659..8247
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8148..8167
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        8678..8823
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     CDS             8866..9903
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPDRE
                     MGEAN"
     primer_bind     complement(9901..9920)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    9945..9993
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt ccgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttacggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta caccaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaataa ccccgccccg ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctcgttta gtgaaccgtc
      601 agatctctag aagctgggta ccagctgcta gcgccaccat ggccgtcatg gcgccccgaa
      661 ccctcgtcct gctactctcg ggggccctgg ccctgaccca gacctgggcg ggctccgact
      721 acaaggacga tgatgacaag ggaggatccg gccctgccgt aatcgagtgt tggtttgttg
      781 aagacgctag tctgaaaggg ctggctaaac ggccaggcgc acttctgttg cggcaaggtc
      841 cgggagaacc acctccacga cccgacttgg accctgaatt gtacctctct gtacacgacc
      901 cagctggagc actccaagct gctttccgac ggtacccaag aggtgctcca gcaccgcatt
      961 gcgagatgag tcggttcgtt cctttgcctg cgagtgcaaa gtgggcatca ggtctgacac
     1021 cagcacaaaa ctgtccacga gcgcttgatg gagcgtggct gatggtttcc ataagctctc
     1081 cggtgctttc actgtcatca ctgctcaggc cacaacctga accccaacaa gaacccgtac
     1141 tgataacaat ggcgacggta gtccttacgg tccttacgca tacaccagca cccagggtgc
     1201 gattgggaca agacgcactc ctcgacctta gctttgcgta catgccacca acctcagagg
     1261 cggcttcctc tcttgcacca ggaccaccac cgttcggcct tgaatggaga cgacagcact
     1321 tggggaaggg ccatttgctg ttggctgcta ccccagggct gaacgggcag atgccggcag
     1381 cacaagaggg agccgtagct tttgctgcgt gggatgatga cgaaccatgg ggaccatgga
     1441 ctggaaacgg gactttctgg ctgccaacag tacagccctt tcaagaagga acgtatctgg
     1501 ccaccatcca tttgccttat ctgcaaggcc aggtcacact tgaactcgct gtctataaac
     1561 cgcctaaggt gagtctcatg cccgcaactt tggctagggc agcacctggc gaagctccac
     1621 ctgaattgct ctgtttggta tcccatttct accccagtgg tggccttgaa gtggaatggg
     1681 aacttagagg aggaccaggt ggtcgatctc agaaagctga agggcagcga tggctctccg
     1741 ctctcaggca ccactcagac ggatctgtat cactgtcagg gcatttgcag ccaccgccag
     1801 ttaccaccga gcagcatgga gctcggtatg cgtgtcgcat tcaccaccct agccttccgg
     1861 cgtcaggaag atcagcggag gtaacacttg aggtcgctgg gttgtccgga cccagcttgg
     1921 aggacagtgt tggtctgttc ctgtccgctt tcctgttgct gggtctgttt aaggcactcg
     1981 gatgggcagc agtttatctc agcacctgta aagacagcaa gaaaaaggca gaatagtaac
     2041 tcgaggccgg caaggccgga tccagacatg ataagataca ttgatgagtt tggacaaacc
     2101 acaactagaa tgcagtgaaa aaaatgcttt atttgtgaaa tttgtgatgc tattgcttta
     2161 tttgtaacca ttataagctg caataaacaa gttaacaaca acaattgcat tcattttatg
     2221 tttcaggttc agggggaggt gtgggaggtt ttttaaagca agtaaaacct ctacaaatgt
     2281 ggtatggctg attatgatcc ggctgcctcg cgcgtttcgg tgatgacggt gaaaacctct
     2341 gacacatgca gctcccggag acggtcacag cttgtctgta agcggatgcc gggagcagac
     2401 aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg gggcgcagcc atgaggtcga
     2461 ctctagagga tcgatccccg ccccggacga actaaacctg actacgacat ctctgcccct
     2521 tcttcgcggg gcagtgcatg taatcccttc agttggttgg tacaacttgc caactgggcc
     2581 ctgttccaca tgtgacacgg ggggggacca aacacaaagg ggttctctga ctgtagttga
     2641 catccttata aatggatgtg cacatttgcc aacactgagt ggctttcatc ctggagcaga
     2701 ctttgcagtc tgtggactgc aacacaacat tgcctttatg tgtaactctt ggctgaagct
     2761 cttacaccaa tgctggggga catgtacctc ccaggggccc aggaagacta cgggaggcta
     2821 caccaacgtc aatcagaggg gcctgtgtag ctaccgataa gcggaccctc aagagggcat
     2881 tagcaatagt gtttataagg cccccttgtt aaccctaaac gggtagcata tgcttcccgg
     2941 gtagtagtat atactatcca gactaaccct aattcaatag catatgttac ccaacgggaa
     3001 gcatatgcta tcgaattagg gttagtaaaa gggtcctaag gaacagcgat atctcccacc
     3061 ccatgagctg tcacggtttt atttacatgg ggtcaggatt ccacgagggt agtgaaccat
     3121 tttagtcaca agggcagtgg ctgaagatca aggagcgggc agtgaactct cctgaatctt
     3181 cgcctgcttc ttcattctcc ttcgtttagc taatagaata actgctgagt tgtgaacagt
     3241 aaggtgtatg tgaggtgctc gaaaacaagg tttcaggtga cgcccccaga ataaaatttg
     3301 gacggggggt tcagtggtgg cattgtgcta tgacaccaat ataaccctca caaacccctt
     3361 gggcaataaa tactagtgta ggaatgaaac attctgaata tctttaacaa tagaaatcca
     3421 tggggtgggg acaagccgta aagactggat gtccatctca cacgaattta tggctatggg
     3481 caacacataa tcctagtgca atatgatact ggggttatta agatgtgtcc caggcaggga
     3541 ccaagacagg tgaaccatgt tgttacactc tatttgtaac aaggggaaag agagtggacg
     3601 ccgacagcag cggactccac tggttgtctc taacaccccc gaaaattaaa cggggctcca
     3661 cgccaatggg gcccataaac aaagacaagt ggccactctt ttttttgaaa ttgtggagtg
     3721 ggggcacgcg tcagccccca cacgccgccc tgcggttttg gactgtaaaa taagggtgta
     3781 ataacttggc tgattgtaac cccgctaacc actgcggtca aaccacttgc ccacaaaacc
     3841 actaatggca ccccggggaa tacctgcata agtaggtggg cgggccaaga taggggcgcg
     3901 attgctgcga tctggaggac aaattacaca cacttgcgcc tgagcgccaa gcacagggtt
     3961 gttggtcctc atattcacga ggtcgctgag agcacggtgg gctaatgttg ccatgggtag
     4021 catatactac ccaaatatct ggatagcata tgctatccta atctatatct gggtagcata
     4081 ggctatccta atctatatct gggtagcata tgctatccta atctatatct gggtagtata
     4141 tgctatccta atytatatct gggtagyata kgctatccta atttatatct gggtagcata
     4201 ggctatccta atctatatct gggtagcata tgctatccta atctatatct gggtagtata
     4261 tgctatccta atctgtatcc gggtagcata tgctatcctc atgcatatac agtcagcata
     4321 tgatacccag tagtagagtg ggagtgctat cctttgcata tgccgccacc tcccaagggg
     4381 gcgtgaattt tcgctgcttg tccttttcct gctggttgct cccattctta ggtgaattta
     4441 aggaggccag gctaaagccg tcgcatgtct gattgctcac caggtaaatg tcgctaatgt
     4501 tttccaacgc gagaaggtgt tgagcgcgga gctgagtgac gtgacaacat gggtatgccc
     4561 aattgcccca tgttgggagg acgaaaatgg tgacaagaca gatggccaga aatacaccaa
     4621 cagcacgcat gatgtctact ggggatttat tctttagtgc gggggaatac acggctttta
     4681 atacgattga gggcgtctcc taacaagtta catcactcct gcccttcctc accctcatct
     4741 ccatcacctc cttcatctcc gtcatctccg tcatcaccct ccgcggcagc cccttccacc
     4801 ataggtggaa accagggagg caaatctact ccatcgtcaa agctgcacac agtcaccctg
     4861 atattgcagg taggagcggg ctttgtcata acaaggtcct taatcgcatc cttcaaaacc
     4921 tcagcaaata tatgagtttg taaaaagacc atgaaataac agacaatgga ctcccttagc
     4981 gggccaggtt gtgggccggg tccaggggcc attccaaagg ggagacgact caatggtgta
     5041 agacgacatt gtggaatagc aagggcagtt cctcgcctta ggttgtaaag ggaggtctta
     5101 ctacctccat atacgaacac accggcgacc caagttcctt cgtcggtagt cctttctacg
     5161 tgactcctag ccaggagagc tcttaaacct tctgcaatgt tctcaaattt cgggttggaa
     5221 cctccttgac cacgatgctt tccaaaccac cctccttttt tgcgcctgcc tccatcaccc
     5281 tgaccccggg gtccagtgct tgggccttct cctgggtcat ctgcggggcc ctgctctatc
     5341 gctcccgggg gcacgtcagg ctcaccatct gggccacctt cttggtggta ttcaaaataa
     5401 tcggcttccc ctacagggtg gaaaaatggc cttctacctg gagggggcct gcgcggtgga
     5461 gacccggatg atgatgactg actactggga ctcctgggcc tcttttctcc acgtccacga
     5521 cctctccccc tggctctttc acgacttccc cccctggctc tttcacgtcc tctaccccgg
     5581 cggcctccac tacctcctcg accccggcct ccactacctc ctcgaccccg gcctccactg
     5641 cctcctcgac cccggcctcc acctcctgct cctgccnnnc ctcctgctcc tgcnncncct
     5701 cctgctcctg cccctcccgc tcctgctcct gctcctgttc caccgtgggt ccctttgcag
     5761 ccaatgcaac ttggacgttt ttggggtctc cggacaccat ctctatgtct tggccctgat
     5821 cctgagccgc ccggggctcc tggtcttccg cctcctcgtc ctcgtcctct tccccgtcct
     5881 cgtccatggt tatcaccccc tcttctttga ggtccactgc cgccggagcc ttctggtcca
     5941 gatgtgtctc ccttctctcc taggccattt ccaggtcctg tacctggccc ctcgtcagac
     6001 atgattcaca ctaaaagaga tcaatagaca tctttattag acgacgctca gtgaatacag
     6061 ggagtgcaga ctcctgcccc ctccaacagc ccccccaccc tcatcccctt catggtcgct
     6121 gtcagacaga tccaggtctg aaaattcccc atcctccgaa ccatcctcgt cctcatcacc
     6181 aattactcgc agcccggaaa actcccgctg aacatcctca agatttgcgt cctgagcctc
     6241 aagccaggcc tcaaattcct cgtccccctt tttgctggac ggtagggatg gggattctcg
     6301 ggacccctcc tcttcctctt caaggtcacc agacagagat gctactgggg caacggaaga
     6361 aaagctgggt gcggcctgtg aggatcagct tatcgatgat aagctgtcaa acatgagaat
     6421 tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata
     6481 atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt
     6541 ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg
     6601 cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt
     6661 cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta
     6721 aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc
     6781 ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa
     6841 gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc
     6901 cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt
     6961 acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact
     7021 gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac
     7081 aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata
     7141 ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta
     7201 ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg
     7261 gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat
     7321 aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt
     7381 aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga
     7441 aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa
     7501 gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag
     7561 gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac
     7621 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc
     7681 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat
     7741 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat
     7801 actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct
     7861 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt
     7921 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg
     7981 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta
     8041 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg
     8101 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg
     8161 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc
     8221 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg
     8281 gccttttgct ggccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg
     8341 gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc
     8401 atccagcctc gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc cccgagatgc
     8461 gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa gggttggttt
     8521 gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg gtgaatccgt
     8581 tagcgaggtg ccgccctgct tcatccccgt ggcccgttgc tcgcgtttgc tggcggtgtc
     8641 cccggaagaa atatatttgc atgtctttag ttctatgatg acacaaaccc cgcccagcgt
     8701 cttgtcattg gcgaattcga acacgcagat gcagtcgggg cggcgcggtc cgaggtccac
     8761 ttcgcatatt aaggtgacgc gtgtggcctc gaacaccgag cgaccctgca gcgacccgct
     8821 taacagcgtc aacagcgtgc cgcagatccc ggggggcaat gagatatgaa aaagcctgaa
     8881 ctcaccgcga cgtctgtcga gaagtttctg atcgaaaagt tcgacagcgt ctccgacctg
     8941 atgcagctct cggagggcga agaatctcgt gctttcagct tcgatgtagg agggcgtgga
     9001 tatgtcctgc gggtaaatag ctgcgccgat ggtttctaca aagatcgtta tgtttatcgg
     9061 cactttgcat cggccgcgct cccgattccg gaagtgcttg acattgggga attcagcgag
     9121 agcctgacct attgcatctc ccgccgtgca cagggtgtca cgttgcaaga cctgcctgaa
     9181 accgaactgc ccgctgttct gcagccggtc gcggaggcca tggatgcgat cgctgcggcc
     9241 gatcttagcc agacgagcgg gttcggccca ttcggaccgc aaggaatcgg tcaatacact
     9301 acatggcgtg atttcatatg cgcgattgct gatccccatg tgtatcactg gcaaactgtg
     9361 atggacgaca ccgtcagtgc gtccgtcgcg caggctctcg atgagctgat gctttgggcc
     9421 gaggactgcc ccgaagtccg gcacctcgtg cacgcggatt tcggctccaa caatgtcctg
     9481 acggacaatg gccgcataac agcggtcatt gactggagcg aggcgatgtt cggggattcc
     9541 caatacgagg tcgccaacat cttcttctgg aggccgtggt tggcttgtat ggagcagcag
     9601 acgcgctact tcgagcggag gcatccggag cttgcaggat cgccgcggct ccgggcgtat
     9661 atgctccgca ttggtcttga ccaactctat cagagcttgg ttgacggcaa tttcgatgat
     9721 gcagcttggg cgcagggtcg atgcgacgca atcgtccgat ccggagccgg gactgtcggg
     9781 cgtacacaaa tcgcccgcag aagcgcggcc gtctggaccg atggctgtgt agaagtactc
     9841 gccgatagtg gaaaccgacg ccccagcact cgtccggatc gggagatggg ggaggctaac
     9901 tgaaacacgg aaggagacaa taccggaagg aacccgcgct atgacggcaa taaaaagaca
     9961 gaataaaacg cacgggtgtt gggtcgtttg ttcataaacg cggggttcgg tcccagggct
    10021 ggcactctgt cgatacccca ccgagacccc attggggcca atacgcccgc gtttcttcct
    10081 tttccccacc ccacccccca agttcgggtg aaggcccagg gctcgcagcc aacgtcgggg
    10141 cggcaggccc tgccatagcc actggccccg tgggttaggg acggggtccc ccatggggaa
    10201 tggtttatgg ttcgtggggg ttattatttt gggcgttgcg tggggtcagg tccacgactg
    10261 gactgagcag acagacccat ggtttttgga tggcctgggc atggaccgca tgtactggcg
    10321 cgacacgaac accgggcgtc tgtggctgcc aaacaccccc gacccccaaa aaccaccgcg
    10381 cggatttctg gcgtgccaag ctagtcgacc aattctcatg tttgacagct tatcatcgca
    10441 gatccgggca acgttgttgc cattgctgca ggcgcagaac tggtaggtat ggaagatcta
    10501 tacattgaat caatattggc aattagccat attagtcatt ggttatatag cataaatcaa
    10561 tattggctat tggccattgc atacgttgta tctatatcat aatatgtaca tttatattgg
    10621 ctcatgtcca atatgaccgc catgtt
//
