LOCUS       sequence-327976-       10637 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of FLAG-tagged Tapasin G15E.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 10637)
  AUTHORS   McShan AC, Devlin CA, Morozov GI, Overall SA, Moschidi D, Akella N, 
            Procko E, Sgourakis NG
  TITLE     TAPBPR promotes antigen loading on MHC-I molecules using a peptide 
            trap.
  JOURNAL   Nat Commun. 2021 May 26;12(1):3174. doi: 10.1038/s41467-021-23225-6.
  PUBMED    34039964
REFERENCE   2  (bases 1 to 10637)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2021-05-26"; volume: "12(1):3174. doi"; pages: " 
            10.1038/s41467-021-23225-6"
COMMENT     Sequence Label: pCEP4-FLAG-Tapasin G15E
FEATURES             Location/Qualifiers
     source          1..10637
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     581..605
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      633..642
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             717..740
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     polyA_signal    2059..2193
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2083..2102)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2137..2156
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(2336..2358)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(6428..6446)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6514..6618
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6619..6687,6688..7479)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6837..6856)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7650..8238
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8139..8158
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        8669..8814
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     CDS             8857..9894
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPDRE
                     MGEAN"
     primer_bind     complement(9892..9911)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    9936..9984
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt ccgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttacggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta caccaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaataa ccccgccccg ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctcgttta gtgaaccgtc
      601 agatctctag aagctgggta ccagctgcta gcgccaccat ggccgtcatg gcgccccgaa
      661 ccctcgtcct gctactctcg ggggccctgg ccctgaccca gacctgggcg ggctccgact
      721 acaaggacga tgatgacaag ggaggatccg gccctgccgt aatcgagtgt tggtttgttg
      781 aagacgctag tgaaaaaggg ctggctaaac ggccaggcgc acttctgttg cggcaaggtc
      841 cgggagaacc acctccacga cccgacttgg accctgaatt gtacctctct gtacacgacc
      901 cagctggagc actccaagct gctttccgac ggtacccaag aggtgctcca gcaccgcatt
      961 gcgagatgag tcggttcgtt cctttgcctg cgagtgcaaa gtgggcatca ggtctgacac
     1021 cagcacaaaa ctgtccacga gcgcttgatg gagcgtggct gatggtttcc ataagctctc
     1081 cggtgctttc actgtcatca ctgctcaggc cacaacctga accccaacaa gaacccgtac
     1141 tgataacaat ggcgacggta gtccttacgg tccttacgca tacaccagca cccagggtgc
     1201 gattgggaca agacgcactc ctcgacctta gctttgcgta catgccacca acctcagagg
     1261 cggcttcctc tcttgcacca ggaccaccac cgttcggcct tgaatggaga cgacagcact
     1321 tggggaaggg ccatttgctg ttggctgcta ccccagggct gaacgggcag atgccggcag
     1381 cacaagaggg agccgtagct tttgctgcgt gggatgatga cgaaccatgg ggaccatgga
     1441 ctggaaacgg gactttctgg ctgccaacag tacagccctt tcaagaagga acgtatctgg
     1501 ccaccatcca tttgccttat ctgcaaggcc aggtcacact tgaactcgct gtctataaac
     1561 cgcctaaggt gagtctcatg cccgcaactt tggctagggc agcacctggc gaagctccac
     1621 ctgaattgct ctgtttggta tcccatttct accccagtgg tggccttgaa gtggaatggg
     1681 aacttagagg aggaccaggt ggtcgatctc agaaagctga agggcagcga tggctctccg
     1741 ctctcaggca ccactcagac ggatctgtat cactgtcagg gcatttgcag ccaccgccag
     1801 ttaccaccga gcagcatgga gctcggtatg cgtgtcgcat tcaccaccct agccttccgg
     1861 cgtcaggaag atcagcggag gtaacacttg aggtcgctgg gttgtccgga cccagcttgg
     1921 aggacagtgt tggtctgttc ctgtccgctt tcctgttgct gggtctgttt aaggcactcg
     1981 gatgggcagc agtttatctc agcacctgta aagacagcaa gaaaaaggca gaatagtaac
     2041 tcgaggccgg caaggccgga tccagacatg ataagataca ttgatgagtt tggacaaacc
     2101 acaactagaa tgcagtgaaa aaaatgcttt atttgtgaaa tttgtgatgc tattgcttta
     2161 tttgtaacca ttataagctg caataaacaa gttaacaaca acaattgcat tcattttatg
     2221 tttcaggttc agggggaggt gtgggaggtt ttttaaagca agtaaaacct ctacaaatgt
     2281 ggtatggctg attatgatcc ggctgcctcg cgcgtttcgg tgatgacggt gaaaacctct
     2341 gacacatgca gctcccggag acggtcacag cttgtctgta agcggatgcc gggagcagac
     2401 aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg gggcgcagcc atgaggtcga
     2461 ctctagagga tcgatccccg ccccggacga actaaacctg actacgacat ctctgcccct
     2521 tcttcgcggg gcagtgcatg taatcccttc agttggttgg tacaacttgc caactgggcc
     2581 ctgttccaca tgtgacacgg ggggggacca aacacaaagg ggttctctga ctgtagttga
     2641 catccttata aatggatgtg cacatttgcc aacactgagt ggctttcatc ctggagcaga
     2701 ctttgcagtc tgtggactgc aacacaacat tgcctttatg tgtaactctt ggctgaagct
     2761 cttacaccaa tgctggggga catgtacctc ccaggggccc aggaagacta cgggaggcta
     2821 caccaacgtc aatcagaggg gcctgtgtag ctaccgataa gcggaccctc aagagggcat
     2881 tagcaatagt gtttataagg cccccttgtt aaccctaaac gggtagcata tgcttcccgg
     2941 gtagtagtat atactatcca gactaaccct aattcaatag catatgttac ccaacgggaa
     3001 gcatatgcta tcgaattagg gttagtaaaa gggtcctaag gaacagcgat atctcccacc
     3061 ccatgagctg tcacggtttt atttacatgg ggtcaggatt ccacgagggt agtgaaccat
     3121 tttagtcaca agggcagtgg ctgaagatca aggagcgggc agtgaactct cctgaatctt
     3181 cgcctgcttc ttcattctcc ttcgtttagc taatagaata actgctgagt tgtgaacagt
     3241 aaggtgtatg tgaggtgctc gaaaacaagg tttcaggtga cgcccccaga ataaaatttg
     3301 gacggggggt tcagtggtgg cattgtgcta tgacaccaat ataaccctca caaacccctt
     3361 gggcaataaa tactagtgta ggaatgaaac attctgaata tctttaacaa tagaaatcca
     3421 tggggtgggg acaagccgta aagactggat gtccatctca cacgaattta tggctatggg
     3481 caacacataa tcctagtgca atatgatact ggggttatta agatgtgtcc caggcaggga
     3541 ccaagacagg tgaaccatgt tgttacactc tatttgtaac aaggggaaag agagtggacg
     3601 ccgacagcag cggactccac tggttgtctc taacaccccc gaaaattaaa cggggctcca
     3661 cgccaatggg gcccataaac aaagacaagt ggccactctt ttttttgaaa ttgtggagtg
     3721 ggggcacgcg tcagccccca cacgccgccc tgcggttttg gactgtaaaa taagggtgta
     3781 ataacttggc tgattgtaac cccgctaacc actgcggtca aaccacttgc ccacaaaacc
     3841 actaatggca ccccggggaa tacctgcata agtaggtggg cgggccaaga taggggcgcg
     3901 attgctgcga tctggaggac aaattacaca cacttgcgcc tgagcgccaa gcacagggtt
     3961 gttggtcctc atattcacga ggtcgctgag agcacggtgg gctaatgttg ccatgggtag
     4021 catatactac ccaaatatct ggatagcata tgctatccta atctatatct gggtagcata
     4081 ggctatccta atctatatct gggtagcata tgctatccta atctatatct gggtagtata
     4141 tgctatccta atttatatct gggtagcata ggctatccta atctatatct gggtagcata
     4201 tgctatccta atctatatct gggtagtata tgctatccta atctgtatcc gggtagcata
     4261 tgctatcctc atgcatatac agtcagcata tgatacccag tagtagagtg ggagtgctat
     4321 cctttgcata tgccgccacc tcccaagggg gcgtgaattt tcgctgcttg tccttttcct
     4381 gctggttgct cccattctta ggtgaattta aggaggccag gctaaagccg tcgcatgtct
     4441 gattgctcac caggtaaatg tcgctaatgt tttccaacgc gagaaggtgt tgagcgcgga
     4501 gctgagtgac gtgacaacat gggtatgccc aattgcccca tgttgggagg acgaaaatgg
     4561 tgacaagaca gatggccaga aatacaccaa cagcacgcat gatgtctact ggggatttat
     4621 tctttagtgc gggggaatac acggctttta atacgattga gggcgtctcc taacaagtta
     4681 catcactcct gcccttcctc accctcatct ccatcacctc cttcatctcc gtcatctccg
     4741 tcatcaccct ccgcggcagc cccttccacc ataggtggaa accagggagg caaatctact
     4801 ccatcgtcaa agctgcacac agtcaccctg atattgcagg taggagcggg ctttgtcata
     4861 acaaggtcct taatcgcatc cttcaaaacc tcagcaaata tatgagtttg taaaaagacc
     4921 atgaaataac agacaatgga ctcccttagc gggccaggtt gtgggccggg tccaggggcc
     4981 attccaaagg ggagacgact caatggtgta agacgacatt gtggaatagc aagggcagtt
     5041 cctcgcctta ggttgtaaag ggaggtctta ctacctccat atacgaacac accggcgacc
     5101 caagttcctt cgtcggtagt cctttctacg tgactcctag ccaggagagc tcttaaacct
     5161 tctgcaatgt tctcaaattt cgggttggaa cctccttgac cacgatgctt tccaaaccac
     5221 cctccttttt tgcgcctgcc tccatcaccc tgaccccggg gtccagtgct tgggccttct
     5281 cctgggtcat ctgcggggcc ctgctctatc gctcccgggg gcacgtcagg ctcaccatct
     5341 gggccacctt cttggtggta ttcaaaataa tcggcttccc ctacagggtg gaaaaatggc
     5401 cttctacctg gagggggcct gcgcggtgga gacccggatg atgatgactg actactggga
     5461 ctcctgggcc tcttttctcc acgtccacga cctctccccc tggctctttc acgacttccc
     5521 cccctggctc tttcacgtcc tctaccccgg cggcctccac tacctcctcg accccggcct
     5581 ccactacctc ctcgaccccg gcctccactg cctcctcgac cccggcctcc acctcctgct
     5641 cctgcccctc ctgctcctgc ccctcctscy sctcctscys ctcctgcccc tcctgctcct
     5701 gcccctcccg ctcctgctcc tgctcctgtt ccaccgtggg tccctttgca gccaatgcaa
     5761 cttggacgtt tttggggtct ccggacacca tctctatgtc ttggccctga tcctgagccg
     5821 cccggggctc ctggtcttcc gcctcctcgt cctcgtcctc ttccccgtcc tcgtccatgg
     5881 ttatcacccc ctcttctttg aggtccactg ccgccggagc cttctggtcc agatgtgtct
     5941 cccttctctc ctaggccatt tccaggtcct gtacctggcc cctcgtcaga catgattcac
     6001 actaaaagag atcaatagac atctttatta gacgacgctc agtgaataca gggagtgcag
     6061 actcctgccc cctccaacag cccccccacc ctcatcccct tcatggtcgc tgtcagacag
     6121 atccaggtct gaaaattccc catcctccga accatcctcg tcctcatcac caattactcg
     6181 cagcccggaa aactcccgct gaacatcctc aagatttgcg tcctgagcct caagccaggc
     6241 ctcaaattcc tcgtccccct ttttgctgga cggtagggat ggggattctc gggacccctc
     6301 ctcttcctct tcaaggtcac cagacagaga tgctactggg gcaacggaag aaaagctggg
     6361 tgcggcctgt gaggatcagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga
     6421 cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct
     6481 tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc
     6541 taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa
     6601 tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt
     6661 gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct
     6721 gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc
     6781 cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta
     6841 tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac
     6901 tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc
     6961 atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac
     7021 ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg
     7081 gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac
     7141 gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc
     7201 gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt
     7261 gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga
     7321 gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc
     7381 cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag
     7441 atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca
     7501 tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc
     7561 ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca
     7621 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc
     7681 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta
     7741 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt
     7801 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc
     7861 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg
     7921 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg
     7981 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag
     8041 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc
     8101 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat
     8161 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg
     8221 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc
     8281 tggccttgaa gctgtccctg atggtcgtca tctacctgcc tggacagcat ggcctgcaac
     8341 gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa tggggaaggc catccagcct
     8401 cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg ccccgagatg cgccgcgtgc
     8461 ggctgctgga gatggcggac gcgatggata tgttctgcca agggttggtt tgcgcattca
     8521 cagttctccg caagaattga ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt
     8581 gccgccctgc ttcatccccg tggcccgttg ctcgcgtttg ctggcggtgt ccccggaaga
     8641 aatatatttg catgtcttta gttctatgat gacacaaacc ccgcccagcg tcttgtcatt
     8701 ggcgaattcg aacacgcaga tgcagtcggg gcggcgcggt ccgaggtcca cttcgcatat
     8761 taaggtgacg cgtgtggcct cgaacaccga gcgaccctgc agcgacccgc ttaacagcgt
     8821 caacagcgtg ccgcagatcc cggggggcaa tgagatatga aaaagcctga actcaccgcg
     8881 acgtctgtcg agaagtttct gatcgaaaag ttcgacagcg tctccgacct gatgcagctc
     8941 tcggagggcg aagaatctcg tgctttcagc ttcgatgtag gagggcgtgg atatgtcctg
     9001 cgggtaaata gctgcgccga tggtttctac aaagatcgtt atgtttatcg gcactttgca
     9061 tcggccgcgc tcccgattcc ggaagtgctt gacattgggg aattcagcga gagcctgacc
     9121 tattgcatct cccgccgtgc acagggtgtc acgttgcaag acctgcctga aaccgaactg
     9181 cccgctgttc tgcagccggt cgcggaggcc atggatgcga tcgctgcggc cgatcttagc
     9241 cagacgagcg ggttcggccc attcggaccg caaggaatcg gtcaatacac tacatggcgt
     9301 gatttcatat gcgcgattgc tgatccccat gtgtatcact ggcaaactgt gatggacgac
     9361 accgtcagtg cgtccgtcgc gcaggctctc gatgagctga tgctttgggc cgaggactgc
     9421 cccgaagtcc ggcacctcgt gcacgcggat ttcggctcca acaatgtcct gacggacaat
     9481 ggccgcataa cagcggtcat tgactggagc gaggcgatgt tcggggattc ccaatacgag
     9541 gtcgccaaca tcttcttctg gaggccgtgg ttggcttgta tggagcagca gacgcgctac
     9601 ttcgagcgga ggcatccgga gcttgcagga tcgccgcggc tccgggcgta tatgctccgc
     9661 attggtcttg accaactcta tcagagcttg gttgacggca atttcgatga tgcagcttgg
     9721 gcgcagggtc gatgcgacgc aatcgtccga tccggagccg ggactgtcgg gcgtacacaa
     9781 atcgcccgca gaagcgcggc cgtctggacc gatggctgtg tagaagtact cgccgatagt
     9841 ggaaaccgac gccccagcac tcgtccggat cgggagatgg gggaggctaa ctgaaacacg
     9901 gaaggagaca ataccggaag gaacccgcgc tatgacggca ataaaaagac agaataaaac
     9961 gcacgggtgt tgggtcgttt gttcataaac gcggggttcg gtcccagggc tggcactctg
    10021 tcgatacccc accgagaccc cattggggcc aatacgcccg cgtttcttcc ttttccccac
    10081 cccacccccc aagttcgggt gaaggcccag ggctcgcagc caacgtcggg gcggcaggcc
    10141 ctgccatagc cactggcccc gtgggttagg gacggggtcc cccatgggga atggtttatg
    10201 gttcgtgggg gttattattt tgggcgttgc gtggggtcag gtccacgact ggactgagca
    10261 gacagaccca tggtttttgg atggcctggg catggaccgc atgtactggc gcgacacgaa
    10321 caccgggcgt ctgtggctgc caaacacccc cgacccccaa aaaccaccgc gcggatttct
    10381 ggcgtgccaa gctagtcgac caattctcat gtttgacagc ttatcatcgc agatccgggc
    10441 aacgttgttg ccattgctgc aggcgcagaa ctggtaggta tggaagatct atacattgaa
    10501 tcaatattgg caattagcca tattagtcat tggttatata gcataaatca atattggcta
    10561 ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc
    10621 aatatgaccg ccatgtt
//
