LOCUS       sequence-327980-        1830 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses a GFP tagged JunB with Flag and EPEA tags. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1830)
  AUTHORS   Ramirez DH, Aonbangkhen C, Wu HY, Naftaly JA, Tang S, O'Meara TR, 
            Woo CM
  TITLE     Engineering a Proximity-Directed O-GlcNAc Transferase for Selective 
            Protein O-GlcNAcylation in Cells.
  JOURNAL   ACS Chem Biol. 2020 Apr 17;15(4):1059-1066. doi: 
            10.1021/acschembio.0c00074. Epub 2020 Mar 2.
  PUBMED    32119511
REFERENCE   2  (bases 1 to 1830)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acschembio.0c00074"; journalName: "ACS Chem Biol"; date: 
            "2020-04-17- 17"; volume: "15"; issue: "4"; pages: "1059-1066"
COMMENT     Sequence Label: (114) pcDNA3.1-GFP-Flag-JunB-EPEA
FEATURES             Location/Qualifiers
     source          1..1830
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(1..3,4..6,7..717)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(1..3,4..6,7..717)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(46..67)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(307..326)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     654..675
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             727..750
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac
       61 ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac
      121 ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc
      181 ctcgtgacca ccctgaccta cggcgtgcag tgcttcagcc gctaccccga ccacatgaag
      241 cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc
      301 ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg
      361 gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac
      421 aagctggagt acaactacaa cagccacaac gtctatatca tggccgacaa gcagaagaac
      481 ggcatcaagg tgaacttcaa gatccgccac aacatcgagg acggcagcgt gcagctcgcc
      541 gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac
      601 tacctgagca cccagtccgc cctgagcaaa gaccccaacg agaagcgcga tcacatggtc
      661 ctgctggagt tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaagggc
      721 gcgccagact acaaggatga cgacgataag ggggaggccg ccgccaagga acggatgtgc
      781 actaaaatgg aacagccctt ctaccacgac gactcataca cagctacggg atacggccgg
      841 gcccctggtg gcctctctct acacgactac aaactcctga aaccgagcct ggcggtcaac
      901 ctggccgacc cctaccggag tctcaaagcg cctggggctc gcggacccgg cccagagggc
      961 ggcggtggcg gcagctactt ttctggtcag ggctcggaca ccggcgcgtc tctcaagctc
     1021 gcctcttcgg agctggaacg cctgattgtc cccaacagca acggcgtgat cacgacgacg
     1081 cctacacccc cgggacagta cttttacccc cgcgggggtg gcagcggtgg aggtgcaggg
     1141 ggcgcagggg gcggcgtcac cgaggagcag gagggcttcg ccgacggctt tgtcaaagcc
     1201 ctggacgatc tgcacaagat gaaccacgtg acacccccca acgtgtccct gggcgctacc
     1261 ggggggcccc cggctgggcc cgggggcgtc tacgccggcc cggagccacc tcccgtttac
     1321 accaacctca gcagctactc cccagcctct gcgtcctcgg gaggcgccgg ggctgccgtc
     1381 gggaccggga gctcgtaccc gacgaccacc atcagctacc tcccacacgc gccgcccttc
     1441 gccggtggcc acccggcgca gctgggcttg ggccgcggcg cctccacctt caaggaggaa
     1501 ccgcagaccg tgccggaggc gcgcagccgg gacgccacgc cgccggtgtc ccccatcaac
     1561 atggaagacc aagagcgcat caaagtggag cgcaagcggc tgcggaaccg gctggcggcc
     1621 accaagtgcc ggaagcggaa gctggagcgc atcgcgcgcc tggaggacaa ggtgaagacg
     1681 ctcaaggccg agaacgcggg gctgtcgagt accgccggcc tcctccggga gcaggtggcc
     1741 cagctcaaac agaaggtcat gacccacgtc agcaacggct gtcagctgct gcttggggtc
     1801 aagggacacg ccttcgaacc tgaagcataa
//
