LOCUS       sequence-327981-       10274 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Control shRNA pLVTHM-shN.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10274)
  AUTHORS   Mayes K, Elsayed Z, Alhazmi A, Waters M, Alkhatib SG, Roberts M, 
            Song C, Peterson K, Chan V, Ailaney N, Malapati P, Blevins T, Lisnic
            B, Dumur CI, Landry JW
  TITLE     BPTF inhibits NK cell activity and the abundance of natural 
            cytotoxicity receptor co-ligands.
  JOURNAL   Oncotarget. 2017 May 12;8(38):64344-64357. doi: 
            10.18632/oncotarget.17834. eCollection 2017 Sep 8.
  PUBMED    28969075
REFERENCE   2  (bases 1 to 10274)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.18632/oncotarget.17834"; journalName: "Oncotarget"; date: 
            "2017-05-12- 12"; volume: "8"; issue: "38"; pages: "64344-64357"
COMMENT     Sequence Label: pLVTHM-shN
FEATURES             Location/Qualifiers
     source          1..10274
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1299..1532
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1717..1761
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        2065..2564
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(2100..2123)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     2497..2516
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             2585..3184
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(2585..2604)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     3081..3101
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    3498..4086
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3551..3571)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3969..3980)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    join(4254..4272,4273..4295,4296..4314,4315..4337,4338..4356,
                     4357..4379,4380..4398,4399..4421,4422..4440,4441..4463,
                     4464..4482,4483..4505,4506..4524)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     promoter        4553..4767
                     /label=H1 promoter
                     /note="human H1 RNA promoter"
     primer_bind     4688..4707
                     /label=H1
                     /note="Human H1 promoter, forward primer"
     protein_bind    complement(4836..4869)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     LTR             4889..5069
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(5094..5112)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(5095..5112)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(5402..5421)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(5635..5654)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5754..5776
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(5814..5832)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5900..6004
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6005..6073,6074..6865)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6223..6242)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7036..7624
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7525..7544
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7778..7795
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(7865..7885)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7870..8199
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8050..8185
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          9333..9398
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             9528..9548
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    9973..10107
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10010..10029)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10064..10083
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgc tgatcttcag acctggagga ggagatatga gggacaattg gagaagtgaa
     1201 ttatataaat ataaagtagt aaaaattgaa ccattaggag tagcacccac caaggcaaag
     1261 agaagagtgg tgcagagaga aaaaagagca gtgggaatag gagctttgtt ccttgggttc
     1321 ttgggagcag caggaagcac tatgggcgca gcgtcaatga cgctgacggt acaggccaga
     1381 caattattgt ctggtatagt gcagcagcag aacaatttgc tgagggctat tgaggcgcaa
     1441 cagcatctgt tgcaactcac agtctggggc atcaagcagc tccaggcaag aatcctggct
     1501 gtggaaagat acctaaagga tcaacagctc ctggggattt ggggttgctc tggaaaactc
     1561 atttgcacca ctgctgtgcc ttggaatgct agttggagta ataaatctct ggaacagatt
     1621 tggaatcaca cgacctggat ggagtgggac agagaaatta acaattacac aagcttaata
     1681 cactccttaa ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga attattggaa
     1741 ttagataaat gggcaagttt gtggaattgg tttaacataa caaattggct gtggtatata
     1801 aaattattca taatgatagt aggaggcttg gtaggtttaa gaatagtttt tgctgtactt
     1861 tctatagtga atagagttag gcagggatat tcaccattat cgtttcagac ccacctccca
     1921 accccgaggg gacccgacag gcccgaagga atagaagaag aaggtggaga gagagacaga
     1981 gacagatcca ttcgattagt gaacggatct cgacggtatc gatcacgaga ctagcctcga
     2041 ccatcgcgat gtcgaaattc taccgggtag gggaggcgct tttcccaagg cagtctggag
     2101 catgcgcttt agcagccccg ctgggcactt ggcgctacac aagtggcctc tggcctcgca
     2161 cacattccac atccaccggt aggcgccaac cggctccgtt ctttggtggc cccttcgcgc
     2221 caccttctac tcctccccta gtcaggaagt tcccccccgc cccgcagctc gcgtcgtgca
     2281 ggacgtgaca aatggaagta gcacgtctca ctagtctcgt gcagatggac agcaccgctg
     2341 agcaatggaa gcgggtaggc ctttggggca gcggccaata gcagctttgc tccttcgctt
     2401 tctgggctca gaggctggga aggggtgggt ccgggggcgg gctcaggggc gggctcaggg
     2461 gcggggcggg cgcccgaagg tcctccggag gcccggcatt ctgcacgctt caaaagcgca
     2521 cgtctgccgc gctgttctcc tcttcctcat ctccgggcct ttcgacctgc agcccaagct
     2581 taccatgacc gagtacaagc ccacggtgcg cctcgccacc cgcgacgacg tccccagggc
     2641 cgtacgcacc ctcgccgccg cgttcgccga ctaccccgcc acgcgccaca ccgtcgatcc
     2701 ggaccgccac atcgagcggg tcaccgagct gcaagaactc ttcctcacgc gcgtcgggct
     2761 cgacatcggc aaggtgtggg tcgcggacga cggcgccgcg gtggcggtct ggaccacgcc
     2821 ggagagcgtc gaagcggggg cggtgttcgc cgagatcggc ccgcgcatgg ccgagttgag
     2881 cggttcccgg ctggccgcgc agcaacagat ggaaggcctc ctggcgccgc accggcccaa
     2941 ggagcccgcg tggttcctgg ccaccgtcgg cgtctcgccc gaccaccagg gcaagggtct
     3001 gggcagcgcc gtcgtgctcc ccggagtgga ggcggccgag cgcgccgggg tgcccgcctt
     3061 cctggagacc tccgcgcccc gcaacctccc cttctacgag cggctcggct tcaccgtcac
     3121 cgccgacgtc gaggtgcccg aaggaccgcg cacctggtgc atgacccgca agcccggtgc
     3181 ctgacgcccg ccccacgacc cgcagcgccc gaccgaaagg agcgcacgac cccatgcatc
     3241 ggcgtctcga gatatcagtg gtccaggctc tagttttgac tcaacaatat caccagctga
     3301 agcctataga gtacgagcca tagataaaat aaaagatttt atttagtctc cagaaaaagg
     3361 ggggaatgaa agaccccacc tgtaggtttg gcaagctagc ttaagtaacg ccattttgca
     3421 aggcatggaa aaatacataa ctgagaatag agaagttcag atcaaggtca ggaacagatg
     3481 gaacagggtc gaatgataat caacctctgg attacaaaat ttgtgaaaga ttgactggta
     3541 ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc
     3601 atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt
     3661 ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg
     3721 ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt
     3781 tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct
     3841 ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg aagctgacgt
     3901 cctttccatg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg tccttctgct
     3961 acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg ccggctctgc
     4021 ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg gatctccctt tgggccgcct
     4081 ccccgcatcg gtacgtatgg ccaggtacct ttaagaccaa tgacttacaa ggcagctgta
     4141 gatcttagcc actttttaaa agaaaagggg ggactggaag ggctaattca ctcccaacga
     4201 agacaagatg ggatcaattc accatgctag tggatccccc tcgagtttac cactccctat
     4261 cagtgataga gaaaagtgaa agtcgagttt accactccct atcagtgata gagaaaagtg
     4321 aaagtcgagt ttaccactcc ctatcagtga tagagaaaag tgaaagtcga gtttaccact
     4381 ccctatcagt gatagagaaa agtgaaagtc gagtttacca ctccctatca gtgatagaga
     4441 aaagtgaaag tcgagtttac cactccctat cagtgataga gaaaagtgaa agtcgagttt
     4501 accactccct atcagtgata gagaaaagtg aaagtcgggg ctgcaggaat tcgaacgctg
     4561 acgtcatcaa cccgctccaa ggaatcgcgg gcccagtgtc actaggcggg aacacccagc
     4621 gcgcgtgcgc cctggcagga agatggctgt gagggacagg ggagtggcgc cctgcaatat
     4681 ttgcatgtcg ctatgtgttc tgggaaatca ccataaacgt gaaatgtctt tggatttggg
     4741 aatcttataa gttctgtatg agaccacgcg tccccgtgcg ttgctagtac caacttcaag
     4801 agatttttta cgcgtatcga taccgtcgca tgggaataac ttcgtatagc atacattata
     4861 cgaagttatg ctgctttttg cttgtactgg gtctctctgg ttagaccaga tctgagcctg
     4921 ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt
     4981 gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc
     5041 cttttagtca gtgtggaaaa tctctagcag catctagaat taattccgtg tattctatag
     5101 tgtcacctaa atcgtatgtg tatgatacat aaggttatgt attaattgta gccgcgttct
     5161 aacgacaata tgtacaagcc taattgtgta gcatctggct tactgaagca gaccctatca
     5221 tctctctcgt aaactgccgt cagagtcggt ttggttggac gaaccttctg agtttctggt
     5281 aacgccgtcc cgcacccgga aatggtcagc gaaccaatca gcagggtcat cgctagccag
     5341 atcctctacg ccggacgcat cgtggccggc atcaccggcg ccacaggtgc ggttgctggc
     5401 gcctatatcg ccgacatcac cgatggggaa gatcgggctc gccacttcgg gctcatgagc
     5461 gcttgtttcg gcgtgggtat ggtggcaggc cccgtggccg ggggactgtt gggcgccatc
     5521 tccttgcatg caccattcct tgcggcggcg gtgctcaacg gcctcaacct actactgggc
     5581 tgcttcctaa tgcaggagtc gcataaggga gagcgtcgaa tggtgcactc tcagtacaat
     5641 ctgctctgat gccgcatagt taagccagcc ccgacacccg ccaacacccg ctgacgcgcc
     5701 ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag
     5761 ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgagacgaa agggcctcgt
     5821 gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg
     5881 cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa
     5941 tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa
     6001 gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct
     6061 tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg
     6121 tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg
     6181 ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt
     6241 atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga
     6301 cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga
     6361 attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac
     6421 gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg
     6481 ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac
     6541 gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct
     6601 agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct
     6661 gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg
     6721 gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat
     6781 ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg
     6841 tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat
     6901 tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct
     6961 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa
     7021 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
     7081 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
     7141 gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta
     7201 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
     7261 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
     7321 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
     7381 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
     7441 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
     7501 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
     7561 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
     7621 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
     7681 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg
     7741 agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc
     7801 ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag
     7861 ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt
     7921 atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca
     7981 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta
     8041 actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga
     8101 ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag
     8161 tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agcttggaca caagacaggc
     8221 ttgcgagata tgtttgagaa taccacttta tcccgcgtca gggagaggca gtgcgtaaaa
     8281 agacgcggac tcatgtgaaa tactggtttt tagtgcgcca gatctctata atctcgcgca
     8341 acctattttc ccctcgaaca ctttttaagc cgtagataaa caggctggga cacttcacat
     8401 gagcgaaaaa tacatcgtca cctgggacat gttgcagatc catgcacgta aactcgcaag
     8461 ccgactgatg ccttctgaac aatggaaagg cattattgcc gtaagccgtg gcggtctgta
     8521 ccgggtgcgt tactggcgcg tgaactgggt attcgtcatg tcgataccgt ttgtatttcc
     8581 agctacgatc acgacaacca gcgcgagctt aaagtgctga aacgcgcaga aggcgatggc
     8641 gaaggcttca tcgttattga tgacctggtg gataccggtg gtactgcggt tgcgattcgt
     8701 gaaatgtatc caaaagcgca ctttgtcacc atcttcgcaa aaccggctgg tcgtccgctg
     8761 gttgatgact atgttgttga tatcccgcaa gatacctgga ttgaacagcc gtgggatatg
     8821 ggcgtcgtat tcgtcccgcc aatctccggt cgctaatctt ttcaacgcct ggcactgccg
     8881 ggcgttgttc tttttaactt caggcgggtt acaatagttt ccagtaagta ttctggaggc
     8941 tgcatccatg acacaggcaa acctgagcga aaccctgttc aaaccccgct ttaaacatcc
     9001 tgaaacctcg acgctagtcc gccgctttaa tcacggcgca caaccgcctg tgcagtcggc
     9061 ccttgatggt aaaaccatcc ctcactggta tcgcatgatt aaccgtctga tgtggatctg
     9121 gcgcggcatt gacccacgcg aaatcctcga cgtccaggca cgtattgtga tgagcgatgc
     9181 cgaacgtacc gacgatgatt tatacgatac ggtgattggc taccgtggcg gcaactggat
     9241 ttatgagtgg gccccggatc tttgtgaagg aaccttactt ctgtggtgtg acataattgg
     9301 acaaactacc tacagagatt taaagctcta aggtaaatat aaaattttta agtgtataat
     9361 gtgttaaact actgattcta attgtttgtg tattttagat tccaacctat ggaactgatg
     9421 aatgggagca gtggtggaat gcctttaatg aggaaaacct gttttgctca gaagaaatgc
     9481 catctagtga tgatgaggct actgctgact ctcaacattc tactcctcca aaaaagaaga
     9541 gaaaggtaga agaccccaag gactttcctt cagaattgct aagttttttg agtcatgctg
     9601 tgtttagtaa tagaactctt gcttgctttg ctatttacac cacaaaggaa aaagctgcac
     9661 tgctatacaa gaaaattatg gaaaaatatt ctgtaacctt tataagtagg cataacagtt
     9721 ataatcataa catactgttt tttcttactc cacacaggca tagagtgtct gctattaata
     9781 actatgctca aaaattgtgt acctttagct ttttaatttg taaaggggtt aataaggaat
     9841 atttgatgta tagtgccttg actagagatc ataatcagcc ataccacatt tgtagaggtt
     9901 ttacttgctt taaaaaacct cccacacctc cccctgaacc tgaaacataa aatgaatgca
     9961 attgttgttg ttaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc
    10021 acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc
    10081 atcaatgtat cttatcatgt ctggatcaac tggataactc aagctaacca aaatcatccc
    10141 aaacttccca ccccataccc tattaccact gccaattacc tgtggtttca tttactctaa
    10201 acctgtgatt cctctgaatt attttcattt taaagaaatt gtatttgtta aatatgtact
    10261 acaaacttag tagt
//
