LOCUS       sequence-327987-       10942 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of FLAG-tagged Tapasin delta2step.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 10942)
  AUTHORS   McShan AC, Devlin CA, Morozov GI, Overall SA, Moschidi D, Akella N, 
            Procko E, Sgourakis NG
  TITLE     TAPBPR promotes antigen loading on MHC-I molecules using a peptide 
            trap.
  JOURNAL   Nat Commun. 2021 May 26;12(1):3174. doi: 10.1038/s41467-021-23225-6.
  PUBMED    34039964
REFERENCE   2  (bases 1 to 10942)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2021-05-26"; volume: "12(1):3174. doi"; pages: " 
            10.1038/s41467-021-23225-6"
COMMENT     Sequence Label: pCEP4-FLAG-Tapasin delta2step
FEATURES             Location/Qualifiers
     source          1..10942
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     581..605
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      633..642
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             717..740
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     polyA_signal    2047..2181
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2071..2090)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2125..2144
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(2324..2346)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      2906..4695
                     /label=oriP
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
     primer_bind     complement(6733..6751)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6819..6923
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6924..6992,6993..7784)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7142..7161)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7955..8543
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8444..8463
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        8974..9119
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     CDS             9162..10199
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPDRE
                     MGEAN"
     primer_bind     complement(10197..10216)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    10241..10289
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt ccgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttacggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta caccaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaataa ccccgccccg ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctcgttta gtgaaccgtc
      601 agatctctag aagctgggta ccagctgcta gcgccaccat ggccgtcatg gcgccccgaa
      661 ccctcgtcct gctactctcg ggggccctgg ccctgaccca gacctgggcg ggctccgact
      721 acaaggacga tgatgacaag ggaggatccg gccctgccgt aatcgagtgt tggtttgttg
      781 aagacggtct ggctaaacgg ccaggcgcac ttctgttgcg gcaaggtccg ggagaaccac
      841 ctccacgacc cgacttggac cctgaattgt acctctctgt acacgaccca gctggagcac
      901 tccaagctgc tttccgacgg tacccaagag gtgctccagc accgcattgc gagatgagtc
      961 ggttcgttcc tttgcctgcg agtgcaaagt gggcatcagg tctgacacca gcacaaaact
     1021 gtccacgagc gcttgatgga gcgtggctga tggtttccat aagctctccg gtgctttcac
     1081 tgtcatcact gctcaggcca caacctgaac cccaacaaga acccgtactg ataacaatgg
     1141 cgacggtagt ccttacggtc cttacgcata caccagcacc cagggtgcga ttgggacaag
     1201 acgcactcct cgaccttagc tttgcgtaca tgccaccaac ctcagaggcg gcttcctctc
     1261 ttgcaccagg accaccaccg ttcggccttg aatggagacg acagcacttg gggaagggcc
     1321 atttgctgtt ggctgctacc ccagggctga acgggcagat gccggcagca caagagggag
     1381 ccgtagcttt tgctgcgtgg gatgatgacg aaccatgggg accatggact ggaaacggga
     1441 ctttctggct gccaacagta cagccctttc aagaaggaac gtatctggcc accatccatt
     1501 tgccttatct gcaaggccag gtcacacttg aactcgctgt ctataaaccg cctaaggtga
     1561 gtctcatgcc cgcaactttg gctagggcag cacctggcga agctccacct gaattgctct
     1621 gtttggtatc ccatttctac cccagtggtg gccttgaagt ggaatgggaa cttagaggag
     1681 gaccaggtgg tcgatctcag aaagctgaag ggcagcgatg gctctccgct ctcaggcacc
     1741 actcagacgg atctgtatca ctgtcagggc atttgcagcc accgccagtt accaccgagc
     1801 agcatggagc tcggtatgcg tgtcgcattc accaccctag ccttccggcg tcaggaagat
     1861 cagcggaggt aacacttgag gtcgctgggt tgtccggacc cagcttggag gacagtgttg
     1921 gtctgttcct gtccgctttc ctgttgctgg gtctgtttaa ggcactcgga tgggcagcag
     1981 tttatctcag cacctgtaaa gacagcaaga aaaaggcaga atagtaactc gaggccggca
     2041 aggccggatc cagacatgat aagatacatt gatgagtttg gacaaaccac aactagaatg
     2101 cagtgaaaaa aatgctttat ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt
     2161 ataagctgca ataaacaagt taacaacaac aattgcattc attttatgtt tcaggttcag
     2221 ggggaggtgt gggaggtttt ttaaagcaag taaaacctct acaaatgtgg tatggctgat
     2281 tatgatccgg ctgcctcgcg cgtttcggtg atgacggtga aaacctctga cacatgcagc
     2341 tcccggagac ggtcacagct tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg
     2401 gcgcgtcagc gggtgttggc gggtgtcggg gcgcagccat gaggtcgact ctagaggatc
     2461 gatccccgcc ccggacgaac taaacctgac tacgacatct ctgccccttc ttcgcggggc
     2521 agtgcatgta atcccttcag ttggttggta caacttgcca actgggccct gttccacatg
     2581 tgacacgggg ggggaccaaa cacaaagggg ttctctgact gtagttgaca tccttataaa
     2641 tggatgtgca catttgccaa cactgagtgg ctttcatcct ggagcagact ttgcagtctg
     2701 tggactgcaa cacaacattg cctttatgtg taactcttgg ctgaagctct tacaccaatg
     2761 ctgggggaca tgtacctccc aggggcccag gaagactacg ggaggctaca ccaacgtcaa
     2821 tcagaggggc ctgtgtagct accgataagc ggaccctcaa gagggcatta gcaatagtgt
     2881 ttataaggcc cccttgttaa ccctaaacgg gtagcatatg cttcccgggt agtagtatat
     2941 actatccaga ctaaccctaa ttcaatagca tatgttaccc aacgggaagc atatgctatc
     3001 gaattagggt tagtaaaagg gtcctaagga acagcgatat ctcccacccc atgagctgtc
     3061 acggttttat ttacatgggg tcaggattcc acgagggtag tgaaccattt tagtcacaag
     3121 ggcagtggct gaagatcaag gagcgggcag tgaactctcc tgaatcttcg cctgcttctt
     3181 cattctcctt cgtttagcta atagaataac tgctgagttg tgaacagtaa ggtgtatgtg
     3241 aggtgctcga aaacaaggtt tcaggtgacg cccccagaat aaaatttgga cggggggttc
     3301 agtggtggca ttgtgctatg acaccaatat aaccctcaca aaccccttgg gcaataaata
     3361 ctagtgtagg aatgaaacat tctgaatatc tttaacaata gaaatccatg gggtggggac
     3421 aagccgtaaa gactggatgt ccatctcaca cgaatttatg gctatgggca acacataatc
     3481 ctagtgcaat atgatactgg ggttattaag atgtgtccca ggcagggacc aagacaggtg
     3541 aaccatgttg ttacactcta tttgtaacaa ggggaaagag agtggacgcc gacagcagcg
     3601 gactccactg gttgtctcta acacccccga aaattaaacg gggctccacg ccaatggggc
     3661 ccataaacaa agacaagtgg ccactctttt ttttgaaatt gtggagtggg ggcacgcgtc
     3721 agcccccaca cgccgccctg cggttttgga ctgtaaaata agggtgtaat aacttggctg
     3781 attgtaaccc cgctaaccac tgcggtcaaa ccacttgccc acaaaaccac taatggcacc
     3841 ccggggaata cctgcataag taggtgggcg ggccaagata ggggcgcgat tgctgcgatc
     3901 tggaggacaa attacacaca cttgcgcctg agcgccaagc acagggttgt tggtcctcat
     3961 attcacgagg tcgctgagag cacggtgggc taatgttgcc atgggtagca tatactaccc
     4021 aaatatctgg atagcatatg ctatcctaat ctatatctgg gtagcatagg ctatcctaat
     4081 ctatatctgg gtagcatatg ctatcctaat ctatatctgg gtagtatatg ctatcctaat
     4141 ttatatctgg gtagcatagg ctatcctaat ctatatctgg gtagcatatg ctatcctaat
     4201 ctatatctgg gtagtatatg ctatcctaat ctgtatccgg gtagcatatg ctatcctaat
     4261 agagattagg gtagtatatg ctatcctaat ttatatctgg gtagcatata ctacccaaat
     4321 atctggatag catatgctat cctaatctat atctgggtag catatgctat cctaatctat
     4381 atctgggtag cataggctat cctaatctat atctgggtag catatgctat cctaatctat
     4441 atctgggtag tatatgctat cctaatttat atctgggtag cataggctat cctaatctat
     4501 atctgggtag catatgctat cctaatctat atctgggtag tatatgctat cctaatctgt
     4561 atccgggtag catatgctat cctcatgcat atacagtcag catatgatac ccagtagtag
     4621 agtgggagtg ctatcctttg catatgccgc cacctcccaa gggggcgtga attttcgctg
     4681 cttgtccttt tcctgctggt tgctcccatt cttaggtgaa tttaaggagg ccaggctaaa
     4741 gccgtcgcat gtctgattgc tcaccaggta aatgtcgcta atgttttcca acgcgagaag
     4801 gtgttgagcg cggagctgag tgacgtgaca acatgggtat gcccaattgc cccatgttgg
     4861 gaggacgaaa atggtgacaa gacagatggc cagaaataca ccaacagcac gcatgatgtc
     4921 tactggggat ttattcttta gtgcggggga atacacggct tttaatacga ttgagggcgt
     4981 ctcctaacaa gttacatcac tcctgccctt cctcaccctc atctccatca cctccttcat
     5041 ctccgtcatc tccgtcatca ccctccgcgg cagccccttc caccataggt ggaaaccagg
     5101 gaggcaaatc tactccatcg tcaaagctgc acacagtcac cctgatattg caggtaggag
     5161 cgggctttgt cataacaagg tccttaatcg catccttcaa aacctcagca aatatatgag
     5221 tttgtaaaaa gaccatgaaa taacagacaa tggactccct tagcgggcca ggttgtgggc
     5281 cgggtccagg ggccattcca aaggggagac gactcaatgg tgtaagacga cattgtggaa
     5341 tagcaagggc agttcctcgc cttaggttgt aaagggaggt cttactacct ccatatacga
     5401 acacaccggc gacccaagtt ccttcgtcgg tagtcctttc tacgtgactc ctagccagga
     5461 gagctcttaa accttctgca atgttctcaa atttcgggtt ggaacctcct tgaccacgat
     5521 gctttccaaa ccaccctcct tttttgcgcc tgcctccatc accctgaccc cggggtccag
     5581 tgcttgggcc ttctcctggg tcatctgcgg ggccctgctc tatcgctccc gggggcacgt
     5641 caggctcacc atctgggcca ccttcttggt ggtattcaaa ataatcggct tcccctacag
     5701 ggtggaaaaa tggccttcta cctggagggg gcctgcgcgg tggagacccg gatgatgatg
     5761 actgactact gggactcctg ggcctctttt ctccacgtcc acgacctctc cccctggctc
     5821 tttcacgact tccccccctg gctctttcac gtcctctacc ccggcggcct ccactacctc
     5881 ctcgaccccg gcctccacta cctcctcgac cccggcctcc actgcctcct cgaccccggc
     5941 ctccacctcc tgctcctgcc cctcctgctc ctgcccctcc tcctgctcct gcccctcctg
     6001 ctcctgcccc tcccgctcct gctcctgctc ctgttccacc gtgggtccct ttgcagccaa
     6061 tgcaacttgg acgtttttgg ggtctccgga caccatctct atgtcttggc cctgatcctg
     6121 agccgcccgg ggctcctggt cttccgcctc ctcgtcctcg tcctcttccc cgtcctcgtc
     6181 catggttatc accccctctt ctttgaggtc cactgccgcc ggagccttct ggtccagatg
     6241 tgtctccctt ctctcctagg ccatttccag gtcctgtacc tggcccctcg tcagacatga
     6301 ttcacactaa aagagatcaa tagacatctt tattagacga cgctcagtga atacagggag
     6361 tgcagactcc tgccccctcc aacagccccc ccaccctcat ccccttcatg gtcgctgtca
     6421 gacagatcca ggtctgaaaa ttccccatcc tccgaaccat cctcgtcctc atcaccaatt
     6481 actcgcagcc cggaaaactc ccgctgaaca tcctcaagat ttgcgtcctg agcctcaagc
     6541 caggcctcaa attcctcgtc cccctttttg ctggacggta gggatgggga ttctcgggac
     6601 ccctcctctt cctcttcaag gtcaccagac agagatgcta ctggggcaac ggaagaaaag
     6661 ctgggtgcgg cctgtgagga tcagcttatc gatgataagc tgtcaaacat gagaattctt
     6721 gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg
     6781 tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat
     6841 ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc
     6901 aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct
     6961 tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag
     7021 atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta
     7081 agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc
     7141 tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca
     7201 tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg
     7261 atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg
     7321 ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca
     7381 tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa
     7441 acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa
     7501 ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata
     7561 aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat
     7621 ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc
     7681 cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata
     7741 gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt
     7801 actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga
     7861 agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag
     7921 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa
     7981 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag
     8041 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg
     8101 tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat
     8161 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta
     8221 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
     8281 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
     8341 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa
     8401 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
     8461 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt
     8521 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct
     8581 tttgctggcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct
     8641 gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc
     8701 agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccccg agatgcgccg
     8761 cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc
     8821 attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc
     8881 gaggtgccgc cctgcttcat ccccgtggcc cgttgctcgc gtttgctggc ggtgtccccg
     8941 gaagaaatat atttgcatgt ctttagttct atgatgacac aaaccccgcc cagcgtcttg
     9001 tcattggcga attcgaacac gcagatgcag tcggggcggc gcggtccgag gtccacttcg
     9061 catattaagg tgacgcgtgt ggcctcgaac accgagcgac cctgcagcga cccgcttaac
     9121 agcgtcaaca gcgtgccgca gatcccgggg ggcaatgaga tatgaaaaag cctgaactca
     9181 ccgcgacgtc tgtcgagaag tttctgatcg aaaagttcga cagcgtctcc gacctgatgc
     9241 agctctcgga gggcgaagaa tctcgtgctt tcagcttcga tgtaggaggg cgtggatatg
     9301 tcctgcgggt aaatagctgc gccgatggtt tctacaaaga tcgttatgtt tatcggcact
     9361 ttgcatcggc cgcgctcccg attccggaag tgcttgacat tggggaattc agcgagagcc
     9421 tgacctattg catctcccgc cgtgcacagg gtgtcacgtt gcaagacctg cctgaaaccg
     9481 aactgcccgc tgttctgcag ccggtcgcgg aggccatgga tgcgatcgct gcggccgatc
     9541 ttagccagac gagcgggttc ggcccattcg gaccgcaagg aatcggtcaa tacactacat
     9601 ggcgtgattt catatgcgcg attgctgatc cccatgtgta tcactggcaa actgtgatgg
     9661 acgacaccgt cagtgcgtcc gtcgcgcagg ctctcgatga gctgatgctt tgggccgagg
     9721 actgccccga agtccggcac ctcgtgcacg cggatttcgg ctccaacaat gtcctgacgg
     9781 acaatggccg cataacagcg gtcattgact ggagcgaggc gatgttcggg gattcccaat
     9841 acgaggtcgc caacatcttc ttctggaggc cgtggttggc ttgtatggag cagcagacgc
     9901 gctacttcga gcggaggcat ccggagcttg caggatcgcc gcggctccgg gcgtatatgc
     9961 tccgcattgg tcttgaccaa ctctatcaga gcttggttga cggcaatttc gatgatgcag
    10021 cttgggcgca gggtcgatgc gacgcaatcg tccgatccgg agccgggact gtcgggcgta
    10081 cacaaatcgc ccgcagaagc gcggccgtct ggaccgatgg ctgtgtagaa gtactcgccg
    10141 atagtggaaa ccgacgcccc agcactcgtc cggatcggga gatgggggag gctaactgaa
    10201 acacggaagg agacaatacc ggaaggaacc cgcgctatga cggcaataaa aagacagaat
    10261 aaaacgcacg ggtgttgggt cgtttgttca taaacgcggg gttcggtccc agggctggca
    10321 ctctgtcgat accccaccga gaccccattg gggccaatac gcccgcgttt cttccttttc
    10381 cccaccccac cccccaagtt cgggtgaagg cccagggctc gcagccaacg tcggggcggc
    10441 aggccctgcc atagccactg gccccgtggg ttagggacgg ggtcccccat ggggaatggt
    10501 ttatggttcg tgggggttat tattttgggc gttgcgtggg gtcaggtcca cgactggact
    10561 gagcagacag acccatggtt tttggatggc ctgggcatgg accgcatgta ctggcgcgac
    10621 acgaacaccg ggcgtctgtg gctgccaaac acccccgacc cccaaaaacc accgcgcgga
    10681 tttctggcgt gccaagctag tcgaccaatt ctcatgtttg acagcttatc atcgcagatc
    10741 cgggcaacgt tgttgccatt gctgcaggcg cagaactggt aggtatggaa gatctataca
    10801 ttgaatcaat attggcaatt agccatatta gtcattggtt atatagcata aatcaatatt
    10861 ggctattggc cattgcatac gttgtatcta tatcataata tgtacattta tattggctca
    10921 tgtccaatat gaccgccatg tt
//
