LOCUS       sequence-327988-        8784 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of candidate TCR #6 TCRbeta cDNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8784)
  AUTHORS   Lee MN, Meyerson M
  TITLE     Antigen identification for HLA class I- and HLA class II-restricted 
            T cell receptors using cytokine-capturing antigen-presenting cells.
  JOURNAL   Sci Immunol. 2021 Jan 22;6(55). pii: 6/55/eabf4001. doi: 
            10.1126/sciimmunol.abf4001.
  PUBMED    33483338
REFERENCE   2  (bases 1 to 8784)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci 
            Immunol."; date: "2021-01-22"; volume: "6(55). pii: 6/55/eabf4001. 
            doi"; pages: " 10.1126/sciimmunol.abf4001"
COMMENT     Sequence Label: CMVTCRb_candidate6
FEATURES             Location/Qualifiers
     source          1..8784
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        65..368
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        369..572
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     523..543
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      606..615
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    1595..2183
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(1648..1668)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2066..2077)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             2255..2488
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    2566..2687
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2603..2622)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2657..2676
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      2727..2862
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(2882..2901)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2883..2901)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2911..2928)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2911..2927)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2920..2942)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      3069..3524
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3156..3175)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3366..3387
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        3550..3654
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3655..3723,3724..4515)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3873..3892)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4686..5274
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5175..5194
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5428..5445
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    5562..5583
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(5598..5603,5604..5621,5622..5628)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5636..5652
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5641..5663
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     5660..5676
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5660..5676
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     5695..5715
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        5697..5715
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        5743..5969
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             5970..6150
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    6197..6322
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    6815..7048
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             7233..7277
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        7452..7952
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     7871..7889
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             7974..8573
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(7974..7993)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     8470..8490
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    8615..8732
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 cccggggtta ttaatagtaa tcaattacgg ggtcattagt tcatagccca tatatggagt
       61 tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc
      121 cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac
      181 gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata
      241 tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc
      301 agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta
      361 ttaccatggt gatgcggttt tggcagtaca tcaatgggcg tggatagcgg tttgactcac
      421 ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttgg caccaaaatc
      481 aacgggactt tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc
      541 gtgtacggtg ggaggtctat ataagcagag ctctctggct aactgtcggg atcaacaagt
      601 ttgccgccac catgggcttc aggctcctct gctgtgtggc cttttgtctc ctgggagcag
      661 gcccagtgga ttctggagtc acacaaaccc caaagcacct gatcacagca actggacagc
      721 gagtgacgct gagatgctcc cctaggtctg gagacctctc tgtgtactgg taccaacaga
      781 gcctggacca gggcctccag ttcctcattc agtattataa tggagaagag agagcaaaag
      841 gaaacattct tgaacgattc tccgcacaac agttccctga cttgcactct gaactaaacc
      901 tgagctctct ggagctgggg gactcagctt tgtatttctg tgccagcagc gccggccagg
      961 gcgtgaccta cgagcagtac ttcgggccgg gcaccaggct cacggtcaca gaggacctga
     1021 aaaacgtgtt cccacccgag gtcgctgtgt ttgagccatc agaagcagag atctcccaca
     1081 cccaaaaggc cacactggtg tgcctggcca caggcttctt ccctgaccac gtggagctga
     1141 gctggtgggt gaatgggaag gaggtgcaca gtggggtctg cacggacccg cagcccctca
     1201 aggagcagcc cgccctcaat gactccagat actgcctgag cagccgcctg agggtctcgg
     1261 ccaccttctg gcagaacccc cgcaaccact ttcgatgcca ggtacaattt tatggtctct
     1321 cggagaatga cgagtggacc caggataggg ccaaacccgt cacccagatc gtcagcgccg
     1381 aggcctgggg tagagcagac tgtggcttta cctcggtgtc ctaccagcaa ggggtcctgt
     1441 ctgccacaat cctctatgag atcctgctag ggaaggccac cctgtatgct gtgctggtca
     1501 gcgcccttgt gttgatggcc atggtcaaga gaaaggattt ctgaaaagtg gtttagtaat
     1561 gagctagcgc tgaccggtgg cgcgttaagt cgacaatcaa cctctggatt acaaaatttg
     1621 tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc
     1681 tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta
     1741 taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt
     1801 ggtgtgcact gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca
     1861 gctcctttcc gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc
     1921 ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt
     1981 gtcggggaaa tcatcgtcct ttccttggct gctcgcctgt gttgccacct ggattctgcg
     2041 cgggacgtcc ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg
     2101 cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat
     2161 ctccctttgg gccgcctccc cgcgtcgact ttaagaccaa tgacttacaa ggcagctgta
     2221 gatcttagcc actttttaaa agaaaagggg ggactggaag ggctaattca ctcccaacga
     2281 agacaagatc tgctttttgc ttgtactggg tctctctggt tagaccagat ctgagcctgg
     2341 gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg
     2401 cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc
     2461 ttttagtcag tgtggaaaat ctctagcagt acgtatagta gttcatgtca tcttattatt
     2521 cagtatttat aacttgcaaa gaaatgaata tcagagagtg agaggaactt gtttattgca
     2581 gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt
     2641 tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggctc
     2701 tagctatccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt
     2761 ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct
     2821 ctgagctatt ccagaagtag tgaggaggct tttttggagg cctagggacg tacccaattc
     2881 gccctatagt gagtcgtatt acgcgcgctc actggccgtc gttttacaac gtcgtgactg
     2941 ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagctg
     3001 gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg
     3061 cgaatgggac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag
     3121 cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt
     3181 tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt
     3241 ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg
     3301 tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt
     3361 taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt
     3421 tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca
     3481 aaaatttaac gcgaatttta acaaaatatt aacgcttaca atttaggtgg cacttttcgg
     3541 ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg
     3601 ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt
     3661 attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt
     3721 gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg
     3781 ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa
     3841 cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt
     3901 gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag
     3961 tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt
     4021 gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga
     4081 ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt
     4141 tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta
     4201 gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg
     4261 caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc
     4321 cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt
     4381 atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg
     4441 gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg
     4501 attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa
     4561 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa
     4621 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
     4681 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
     4741 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
     4801 ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac
     4861 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
     4921 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
     4981 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
     5041 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
     5101 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
     5161 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
     5221 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
     5281 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt
     5341 cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc
     5401 gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc
     5461 ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac
     5521 aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact
     5581 cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg tggaattgtg
     5641 agcggataac aatttcacac aggaaacagc tatgaccatg attacgccaa gcgcgcaatt
     5701 aaccctcact aaagggaaca aaagctggag ctgcaagctt aatgtagtct tatgcaatac
     5761 tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca tgccttacaa ggagagaaaa
     5821 agcaccgtgc atgccgattg gtggaagtaa ggtggtacga tcgtgcctta ttaggaaggc
     5881 aacagacggg tctgacatgg attggacgaa ccactgaatt gccgcattgc agagatattg
     5941 tatttaagtg cctagctcga tacataaacg ggtctctctg gttagaccag atctgagcct
     6001 gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag
     6061 tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac
     6121 ccttttagtc agtgtggaaa atctctagca gtggcgcccg aacagggact tgaaagcgaa
     6181 agggaaacca gaggagctct ctcgacgcag gactcggctt gctgaagcgc gcacggcaag
     6241 aggcgagggg cggcgactgg tgagtacgcc aaaaattttg actagcggag gctagaagga
     6301 gagagatggg tgcgagagcg tcagtattaa gcgggggaga attagatcgc gatgggaaaa
     6361 aattcggtta aggccagggg gaaagaaaaa atataaatta aaacatatag tatgggcaag
     6421 cagggagcta gaacgattcg cagttaatcc tggcctgtta gaaacatcag aaggctgtag
     6481 acaaatactg ggacagctac aaccatccct tcagacagga tcagaagaac ttagatcatt
     6541 atataataca gtagcaaccc tctattgtgt gcatcaaagg atagagataa aagacaccaa
     6601 ggaagcttta gacaagatag aggaagagca aaacaaaagt aagaccaccg cacagcaagc
     6661 ggccgctgat cttcagacct ggaggaggag atatgaggga caattggaga agtgaattat
     6721 ataaatataa agtagtaaaa attgaaccat taggagtagc acccaccaag gcaaagagaa
     6781 gagtggtgca gagagaaaaa agagcagtgg gaataggagc tttgttcctt gggttcttgg
     6841 gagcagcagg aagcactatg ggcgcagcgt caatgacgct gacggtacag gccagacaat
     6901 tattgtctgg tatagtgcag cagcagaaca atttgctgag ggctattgag gcgcaacagc
     6961 atctgttgca actcacagtc tggggcatca agcagctcca ggcaagaatc ctggctgtgg
     7021 aaagatacct aaaggatcaa cagctcctgg ggatttgggg ttgctctgga aaactcattt
     7081 gcaccactgc tgtgccttgg aatgctagtt ggagtaataa atctctggaa cagatttgga
     7141 atcacacgac ctggatggag tgggacagag aaattaacaa ttacacaagc ttaatacact
     7201 ccttaattga agaatcgcaa aaccagcaag aaaagaatga acaagaatta ttggaattag
     7261 ataaatgggc aagtttgtgg aattggttta acataacaaa ttggctgtgg tatataaaat
     7321 tattcataat gatagtagga ggcttggtag gtttaagaat agtttttgct gtactttcta
     7381 tagtgaatag agttaggcag ggatattcac cattatcgtt tcagacccac ctcccaaccc
     7441 cgaggggacc cttgcgcctt ttccaaggca gccctgggtt tgcgcaggga cgcggctgct
     7501 ctgggcgtgg ttccgggaaa cgcagcggcg ccgaccctgg gtctcgcaca ttcttcacgt
     7561 ccgttcgcag cgtcacccgg atcttcgccg ctacccttgt gggccccccg gcgacgcttc
     7621 ctgctccgcc cctaagtcgg gaaggttcct tgcggttcgc ggcgtgccgg acgtgacaaa
     7681 cggaagccgc acgtctcact agtaccctcg cagacggaca gcgccaggga gcaatggcag
     7741 cgcgccgacc gcgatgggct gtggccaata gcggctgctc agcagggcgc gccgagagca
     7801 gcggccggga aggggcggtg cgggaggcgg ggtgtggggc ggtagtgtgg gccctgttcc
     7861 tgcccgcgcg gtgttccgca ttctgcaagc ctccggagcg cacgtcggca gtcggctccc
     7921 tcgttgaccg aatcaccgac ctctctcccc agggggtacc accggagctt accatgaccg
     7981 agtacaagcc cacggtgcgc ctcgccaccc gcgacgacgt ccccagggcc gtacgcaccc
     8041 tcgccgccgc gttcgccgac taccccgcca cgcgccacac cgtcgatccg gaccgccaca
     8101 tcgagcgggt caccgagctg caagaactct tcctcacgcg cgtcgggctc gacatcggca
     8161 aggtgtgggt cgcggacgac ggcgccgccg tggcggtctg gaccacgccg gagagcgtcg
     8221 aagcgggggc ggtgttcgcc gagatcggcc cgcgcatggc cgagttgagc ggttcccggc
     8281 tggccgcgca gcaacagatg gaaggcctcc tggcgccgca ccggcccaag gagcccgcgt
     8341 ggttcctggc caccgtcggc gtctcgcccg accaccaggg caagggtctg ggcagcgccg
     8401 tcgtgctccc cggagtggag gcggccgagc gcgccggggt gcccgccttc ctggagacct
     8461 ccgcgccccg caacctcccc ttctacgagc ggctcggctt caccgtcacc gccgacgtcg
     8521 aggtgcccga aggaccgcgc acctggtgca tgacccgcaa gcccggtgcc tgagaattct
     8581 agatcttgag acaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg
     8641 ggtacagtgc aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat
     8701 tacaaaaaca aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc
     8761 cactttggcg ccggctcgag gggg
//
