LOCUS       sequence-327991-       10673 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of FLAG-tagged Tapasin with TAPBPR a.a. 22-35.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 10673)
  AUTHORS   McShan AC, Devlin CA, Morozov GI, Overall SA, Moschidi D, Akella N, 
            Procko E, Sgourakis NG
  TITLE     TAPBPR promotes antigen loading on MHC-I molecules using a peptide 
            trap.
  JOURNAL   Nat Commun. 2021 May 26;12(1):3174. doi: 10.1038/s41467-021-23225-6.
  PUBMED    34039964
REFERENCE   2  (bases 1 to 10673)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2021-05-26"; volume: "12(1):3174. doi"; pages: " 
            10.1038/s41467-021-23225-6"
COMMENT     Sequence Label: pCEP4-FLAG-Tapasin-TAPBPR 22-35
FEATURES             Location/Qualifiers
     source          1..10673
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(2144..2162)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        2230..2334
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2335..2403,2404..3195)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2553..2572)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3366..3954
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3855..3874
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        4385..4530
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     CDS             4573..5610
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPDRE
                     MGEAN"
     primer_bind     complement(5608..5627)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    5652..5700
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     enhancer        6354..6733
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        6734..6937
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     6888..6908
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     6934..6958
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      6986..6995
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             7070..7093
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     polyA_signal    8427..8561
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8451..8470)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     8505..8524
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(8704..8726)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
ORIGIN
        1 tatacagtca gcatatgata cccagtagta gagtgggagt gctatccttt gcatatgccg
       61 ccacctccca agggggcgtg aattttcgct gcttgtcctt ttcctgctgg ttgctcccat
      121 tcttaggtga atttaaggag gccaggctaa agccgtcgca tgtctgattg ctcaccaggt
      181 aaatgtcgct aatgttttcc aacgcgagaa ggtgttgagc gcggagctga gtgacgtgac
      241 aacatgggta tgcccaattg ccccatgttg ggaggacgaa aatggtgaca agacagatgg
      301 ccagaaatac accaacagca cgcatgatgt ctactgggga tttattcttt agtgcggggg
      361 aatacacggc ttttaatacg attgagggcg tctcctaaca agttacatca ctcctgccct
      421 tcctcaccct catctccatc acctccttca tctccgtcat ctccgtcatc accctccgcg
      481 gcagcccctt ccaccatagg tggaaaccag ggaggcaaat ctactccatc gtcaaagctg
      541 cacacagtca ccctgatatt gcaggtagga gcgggctttg tcataacaag gtccttaatc
      601 gcatccttca aaacctcagc aaatatatga gtttgtaaaa agaccatgaa ataacagaca
      661 atggactccc ttagcgggcc aggttgtggg ccgggtccag gggccattcc aaaggggaga
      721 cgactcaatg gtgtaagacg acattgtgga atagcaaggg cagttcctcg ccttaggttg
      781 taaagggagg tcttactacc tccatatacg aacacaccgg cgacccaagt tccttcgtcg
      841 gtagtccttt ctacgtgact cctagccagg agagctctta aaccttctgc aatgttctca
      901 aatttcgggt tggaacctcc ttgaccacga tgctttccaa accaccctcc ttttttgcgc
      961 ctgcctccat caccctgacc ccggggtcca gtgcttgggc cttctcctgg gtcatctgcg
     1021 gggccctgct ctatcgctcc cgggggcacg tcaggctcac catctgggcc accttcttgg
     1081 tggtattcaa aataatcggc ttcccctaca gggtggaaaa atggccttct acctggaggg
     1141 ggcctgcgcg gtggagaccc ggatgatgat gactgactac tgggactcct gggcctcttt
     1201 tctccacgtc cacgacctct ccccctggct ctttcacgac ttccccccct ggctctttca
     1261 cgtcctctac cccggcggcc tccactacct cctcgacccc ggcctccact acctcctcga
     1321 ccccggcctc cactgcctcc tcgaccccgg cctccacctc ctgctcctgc ccctcctgct
     1381 cctgcccctc ctcctgctcc tgcccctcct gctcctgccc ctcccgctcc tgctcctgct
     1441 cctgttccac cgtgggtccc tttgcagcca atgcaacttg gacgtttttg gggtctccgg
     1501 acaccatctc tatgtcttgg ccctgatcct gagccgcccg gggctcctgg tcttccgcct
     1561 cctcgtcctc gtcctcttcc ccgtcctcgt ccatggttat caccccctct tctttgaggt
     1621 ccactgccgc cggagccttc tggtccagat gtgtctccct tctctcctag gccatttcca
     1681 ggtcctgtac ctggcccctc gtcagacatg attcacacta aaagagatca atagacatct
     1741 ttattagacg acgctcagtg aatacaggga gtgcagactc ctgccccctc caacagcccc
     1801 cccaccctca tccccttcat ggtcgctgtc agacagatcc aggtctgaaa attccccatc
     1861 ctccgaacca tcctcgtcct catcaccaat tactcgcagc ccggaaaact cccgctgaac
     1921 atcctcaaga tttgcgtcct gagcctcaag ccaggcctca aattcctcgt cccccttttt
     1981 gctggacggt agggatgggg attctcggga cccctcctct tcctcttcaa ggtcaccaga
     2041 cagagatgct actggggcaa cggaagaaaa gctgggtgcg gcctgtgagg atcagcttat
     2101 cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta
     2161 tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg
     2221 ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg
     2281 ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt
     2341 attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt
     2401 gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg
     2461 ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa
     2521 cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt
     2581 gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag
     2641 tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt
     2701 gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga
     2761 ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt
     2821 tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca
     2881 gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg
     2941 caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc
     3001 cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt
     3061 atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg
     3121 gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg
     3181 attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa
     3241 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa
     3301 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
     3361 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
     3421 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
     3481 ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac
     3541 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
     3601 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
     3661 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
     3721 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
     3781 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
     3841 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
     3901 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
     3961 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttgaagctg tccctgatgg
     4021 tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa
     4081 gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg
     4141 tagcccagcg cgtcggcccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga
     4201 tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg
     4261 ctccaattct tggagtggtg aatccgttag cgaggtgccg ccctgcttca tccccgtggc
     4321 ccgttgctcg cgtttgctgg cggtgtcccc ggaagaaata tatttgcatg tctttagttc
     4381 tatgatgaca caaaccccgc ccagcgtctt gtcattggcg aattcgaaca cgcagatgca
     4441 gtcggggcgg cgcggtccga ggtccacttc gcatattaag gtgacgcgtg tggcctcgaa
     4501 caccgagcga ccctgcagcg acccgcttaa cagcgtcaac agcgtgccgc agatcccggg
     4561 gggcaatgag atatgaaaaa gcctgaactc accgcgacgt ctgtcgagaa gtttctgatc
     4621 gaaaagttcg acagcgtctc cgacctgatg cagctctcgg agggcgaaga atctcgtgct
     4681 ttcagcttcg atgtaggagg gcgtggatat gtcctgcggg taaatagctg cgccgatggt
     4741 ttctacaaag atcgttatgt ttatcggcac tttgcatcgg ccgcgctccc gattccggaa
     4801 gtgcttgaca ttggggaatt cagcgagagc ctgacctatt gcatctcccg ccgtgcacag
     4861 ggtgtcacgt tgcaagacct gcctgaaacc gaactgcccg ctgttctgca gccggtcgcg
     4921 gaggccatgg atgcgatcgc tgcggccgat cttagccaga cgagcgggtt cggcccattc
     4981 ggaccgcaag gaatcggtca atacactaca tggcgtgatt tcatatgcgc gattgctgat
     5041 ccccatgtgt atcactggca aactgtgatg gacgacaccg tcagtgcgtc cgtcgcgcag
     5101 gctctcgatg agctgatgct ttgggccgag gactgccccg aagtccggca cctcgtgcac
     5161 gcggatttcg gctccaacaa tgtcctgacg gacaatggcc gcataacagc ggtcattgac
     5221 tggagcgagg cgatgttcgg ggattcccaa tacgaggtcg ccaacatctt cttctggagg
     5281 ccgtggttgg cttgtatgga gcagcagacg cgctacttcg agcggaggca tccggagctt
     5341 gcaggatcgc cgcggctccg ggcgtatatg ctccgcattg gtcttgacca actctatcag
     5401 agcttggttg acggcaattt cgatgatgca gcttgggcgc agggtcgatg cgacgcaatc
     5461 gtccgatccg gagccgggac tgtcgggcgt acacaaatcg cccgcagaag cgcggccgtc
     5521 tggaccgatg gctgtgtaga agtactcgcc gatagtggaa accgacgccc cagcactcgt
     5581 ccggatcggg agatggggga ggctaactga aacacggaag gagacaatac cggaaggaac
     5641 ccgcgctatg acggcaataa aaagacagaa taaaacgcac gggtgttggg tcgtttgttc
     5701 ataaacgcgg ggttcggtcc cagggctggc actctgtcga taccccaccg agaccccatt
     5761 ggggccaata cgcccgcgtt tcttcctttt ccccacccca ccccccaagt tcgggtgaag
     5821 gcccagggct cgcagccaac gtcggggcgg caggccctgc catagccact ggccccgtgg
     5881 gttagggacg gggtccccca tggggaatgg tttatggttc gtgggggtta ttattttggg
     5941 cgttgcgtgg ggtcaggtcc acgactggac tgagcagaca gacccatggt ttttggatgg
     6001 cctgggcatg gaccgcatgt actggcgcga cacgaacacc gggcgtctgt ggctgccaaa
     6061 cacccccgac ccccaaaaac caccgcgcgg atttctggcg tgccaagcta gtcgaccaat
     6121 tctcatgttt gacagcttat catcgcagat ccgggcaacg ttgttgccat tgctgcaggc
     6181 gcagaactgg taggtatgga agatctatac attgaatcaa tattggcaat tagccatatt
     6241 agtcattggt tatatagcat aaatcaatat tggctattgg ccattgcata cgttgtatct
     6301 atatcataat atgtacattt atattggctc atgtccaata tgaccgccat gttgacattg
     6361 attattgact agttattaat agtaatcaat tacggggtca ttagttcata gcccatatat
     6421 ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc
     6481 ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca
     6541 ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta
     6601 tcatatgcca agtccgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta
     6661 tgcccagtac atgaccttac gggactttcc tacttggcag tacatctacg tattagtcat
     6721 cgctattacc atggtgatgc ggttttggca gtacaccaat gggcgtggat agcggtttga
     6781 ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca
     6841 aaatcaacgg gactttccaa aatgtcgtaa taaccccgcc ccgttgacgc aaatgggcgg
     6901 taggcgtgta cggtgggagg tctatataag cagagctcgt ttagtgaacc gtcagatctc
     6961 tagaagctgg gtaccagctg ctagcgccac catggccgtc atggcgcccc gaaccctcgt
     7021 cctgctactc tcgggggccc tggccctgac ccagacctgg gcgggctccg actacaagga
     7081 cgatgatgac aagggaggat ccggccctgc cgtaatcgag tgttggtttg ttaaggacgg
     7141 tgcgcaccgt ggagctctcg ccagcagtga ggacaaacgg ccaggcgcac ttctgttgcg
     7201 gcaaggtccg ggagaaccac ctccacgacc cgacttggac cctgaattgt acctctctgt
     7261 acacgaccca gctggagcac tccaagctgc tttccgacgg tacccaagag gtgctccagc
     7321 accgcattgc gagatgagtc ggttcgttcc tttgcctgcg agtgcaaagt gggcatcagg
     7381 tctgacacca gcacaaaact gtccacgagc gcttgatgga gcgtggctga tggtttccat
     7441 aagctctccg gtgctttcac tgtcatcact gctcaggcca caacctgaac cccaacaaga
     7501 acccgtactg ataacaatgg cgacggtagt ccttacggtc cttacgcata caccagcacc
     7561 cagggtgcga ttgggacaag acgcactcct cgaccttagc tttgcgtaca tgccaccaac
     7621 ctcagaggcg gcttcctctc ttgcaccagg accaccaccg ttcggccttg aatggagacg
     7681 acagcacttg gggaagggcc atttgctgtt ggctgctacc ccagggctga acgggcagat
     7741 gccggcagca caagagggag ccgtagcttt tgctgcgtgg gatgatgacg aaccatgggg
     7801 accatggact ggaaacggga ctttctggct gccaacagta cagccctttc aagaaggaac
     7861 gtatctggcc accatccatt tgccttatct gcaaggccag gtcacacttg aactcgctgt
     7921 ctataaaccg cctaaggtga gtctcatgcc cgcaactttg gctagggcag cacctggcga
     7981 agctccacct gaattgctct gtttggtatc ccatttctac cccagtggtg gccttgaagt
     8041 ggaatgggaa cttagaggag gaccaggtgg tcgatctcag aaagctgaag ggcagcgatg
     8101 gctctccgct ctcaggcacc actcagacgg atctgtatca ctgtcagggc atttgcagcc
     8161 accgccagtt accaccgagc agcatggagc tcggtatgcg tgtcgcattc accaccctag
     8221 ccttccggcg tcaggaagat cagcggaggt aacacttgag gtcgctgggt tgtccggacc
     8281 cagcttggag gacagtgttg gtctgttcct gtccgctttc ctgttgctgg gtctgtttaa
     8341 ggcactcgga tgggcagcag tttatctcag cacctgtaaa gacagcaaga aaaaggcaga
     8401 atagtaactc gaggccggca aggccggatc cagacatgat aagatacatt gatgagtttg
     8461 gacaaaccac aactagaatg cagtgaaaaa aatgctttat ttgtgaaatt tgtgatgcta
     8521 ttgctttatt tgtaaccatt ataagctgca ataaacaagt taacaacaac aattgcattc
     8581 attttatgtt tcaggttcag ggggaggtgt gggaggtttt ttaaagcaag taaaacctct
     8641 acaaatgtgg tatggctgat tatgatccgg ctgcctcgcg cgtttcggtg atgacggtga
     8701 aaacctctga cacatgcagc tcccggagac ggtcacagct tgtctgtaag cggatgccgg
     8761 gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg gcgcagccat
     8821 gaggtcgact ctagaggatc gatccccgcc ccggacgaac taaacctgac tacgacatct
     8881 ctgccccttc ttcgcggggc agtgcatgta atcccttcag ttggttggta caacttgcca
     8941 actgggccct gttccacatg tgacacgggg ggggaccaaa cacaaagggg ttctctgact
     9001 gtagttgaca tccttataaa tggatgtgca catttgccaa cactgagtgg ctttcatcct
     9061 ggagcagact ttgcagtctg tggactgcaa cacaacattg cctttatgtg taactcttgg
     9121 ctgaagctct tacaccaatg ctgggggaca tgtacctccc aggggcccag gaagactacg
     9181 ggaggctaca ccaacgtcaa tcagaggggc ctgtgtagct accgataagc ggaccctcaa
     9241 gagggcatta gcaatagtgt ttataaggcc cccttgttaa ccctaaacgg gtagcatatg
     9301 cttcccgggt agtagtatat actatccaga ctaaccctaa ttcaatagca tatgttaccc
     9361 aacgggaagc atatgctatc gaattagggt tagtaaaagg gtcctaagga acagcgatat
     9421 ctcccacccc atgagctgtc acggttttat ttacatgggg tcaggattcc acgagggtag
     9481 tgaaccattt tagtcacaag ggcagtggct gaagatcaag gagcgggcag tgaactctcc
     9541 tgaatcttcg cctgcttctt cattctcctt cgtttagcta atagaataac tgctgagttg
     9601 tgaacagtaa ggtgtatgtg aggtgctcga aaacaaggtt tcaggtgacg cccccagaat
     9661 aaaatttgga cggggggttc agtggtggca ttgtgctatg acaccaatat aaccctcaca
     9721 aaccccttgg gcaataaata ctagtgtagg aatgaaacat tctgaatatc tttaacaata
     9781 gaaatccatg gggtggggac aagccgtaaa gactggatgt ccatctcaca cgaatttatg
     9841 gctatgggca acacataatc ctagtgcaat atgatactgg ggttattaag atgtgtccca
     9901 ggcagggacc aagacaggtg aaccatgttg ttacactcta tttgtaacaa ggggaaagag
     9961 agtggacgcc gacagcagcg gactccactg gttgtctcta acacccccga aaattaaacg
    10021 gggctccacg ccaatggggc ccataaacaa agacaagtgg ccactctttt ttttgaaatt
    10081 gtggagtggg ggcacgcgtc agcccccaca cgccgccctg cggttttgga ctgtaaaata
    10141 agggtgtaat aacttggctg attgtaaccc cgctaaccac tgcggtcaaa ccacttgccc
    10201 acaaaaccac taatggcacc ccggggaata cctgcataag taggtgggcg ggccaagata
    10261 ggggcgcgat tgctgcgatc tggaggacaa attacacaca cttgcgcctg agcgccaagc
    10321 acagggttgt tggtcctcat attcacgagg tcgctgagag cacggtgggc taatgttgcc
    10381 atgggtagca tatactaccc aaatatctgg atagcatatg ctatcctaat ctatatctgg
    10441 gtagcatagg ctatcctaat ctatatctgg gtagcatatg ctatcctaat ctatatctgg
    10501 gtagyatatg ctatcctaat ytatatctgg gtagyatakg ctatcctaat ytatatctgg
    10561 gtagcatagg ctatcctaat ctatatctgg gtagcatatg ctatcctaat ctatatctgg
    10621 gtagtatatg ctatcctaat ctgtatccgg gtagcatatg ctatcctcat gca
//
