LOCUS       sequence-327993-       11119 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of FLAG-tagged TAPBPR with tapasin CT S333A.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 11119)
  AUTHORS   McShan AC, Devlin CA, Morozov GI, Overall SA, Moschidi D, Akella N, 
            Procko E, Sgourakis NG
  TITLE     TAPBPR promotes antigen loading on MHC-I molecules using a peptide 
            trap.
  JOURNAL   Nat Commun. 2021 May 26;12(1):3174. doi: 10.1038/s41467-021-23225-6.
  PUBMED    34039964
REFERENCE   2  (bases 1 to 11119)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2021-05-26"; volume: "12(1):3174. doi"; pages: " 
            10.1038/s41467-021-23225-6"
COMMENT     Sequence Label: pCEP4-FLAG-TAPBPR-tapasinCT S333A
FEATURES             Location/Qualifiers
     source          1..11119
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     581..605
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      633..642
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             717..740
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     polyA_signal    2224..2358
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2248..2267)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2302..2321
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(2501..2523)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      3083..4872
                     /label=oriP
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
     primer_bind     complement(6910..6928)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6996..7100
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7101..7169,7170..7961)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7319..7338)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8132..8720
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8621..8640
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        9151..9296
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     CDS             9339..10376
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPDRE
                     MGEAN"
     primer_bind     complement(10374..10393)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    10418..10466
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt ccgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttacggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta caccaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaataa ccccgccccg ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctcgttta gtgaaccgtc
      601 agatctctag aagctgggta ccagctgcta gcgccaccat ggccgtcatg gcgccccgaa
      661 ccctcgtcct gctactctcg ggggccctgg ccctgaccca gacctgggcg ggctccgact
      721 acaaggacga tgatgacaag ggaggatcca agccccaccc agcagagggg cagtggcggg
      781 cagtggacgt ggtcctagac tgtttcctgg tgaaggacgg tgcgcaccgt ggagctctcg
      841 ccagcagtga ggacagggca agggcctccc ttgtgctgaa gcaggtgcca gtgctggacg
      901 atggctccct ggaggacttc accgatttcc aagggggcac actggcccaa gatgacccac
      961 ctattatctt tgaggcctca gtggacctgg tccagattcc ccaggccgag gccttgctcc
     1021 atgctgactg cagtgggaag gaggtgacct gtgagatctc ccgctacttt ctccagatga
     1081 cagagaccac tgttaagaca gcagcttggt tcatggccaa cgtgcaggtc tctggagggg
     1141 gacctagcat ctccttggtg atgaagactc ccagggtcgc caagaatgag gtgctctggc
     1201 acccaacgct gaacttgcca ctgagccccc aggggactgt gcgaactgca gtggagttcc
     1261 aggtgatgac acagacccaa tccctgagct tcctgctggg gtcctcagcc tccttggact
     1321 gtggcttctc catggcaccg ggcttggacc tcatcagtgt ggagtggcga ctgcagcaca
     1381 agggcagggg tcagttggtg tacagctgga ccgcagggca ggggcaggct gtgcggaagg
     1441 gcgctaccct ggagcctgca caactgggca tggccaggga tgcctccctc accctgcccg
     1501 gcctcactat acaggacgag gggacctaca tttgccagat caccacctct ctgtaccgag
     1561 ctcagcagat catccagctc aacatccaag cttcccctaa agtacgactg agcttggcaa
     1621 acgaagctct gctgcccacc ctcatctgcg acattgctgg ctattaccct ctggatgtgg
     1681 tggtgacgtg gacccgagag gagctgggtg gatccccagc ccaagtctct ggtgcctcct
     1741 tctccgccct caggcaaagc gtggcaggca cctacagcat ctcctcctct ctcaccgcag
     1801 aacctggctc tgcaggtgcc acttacacct gccaggtcac acacatctct ctggaggagc
     1861 cccttggggc cagcacccag gttgtcccac cagagcggag atcagggccc tcccttgagg
     1921 acagcgtagg ccttttcctg tctgcctttc ttctgcttgg gctcttcaag gcactgggct
     1981 gggctgctgt ctacctgtcc acctgcaagg attcaaagaa ggtacagtgc tccacctctc
     2041 tgtatctttc ccttgtcact ttatctcctc atcctatctc aaaacccatg gagggaggct
     2101 gctggtgtgg taggcagaac ctaggcttgg aattcacact gatctgggtt aaaacctggc
     2161 actatatcct aactgtagga ctctttgagc atgctactta atgactcgag gccggcaagg
     2221 ccggatccag acatgataag atacattgat gagtttggac aaaccacaac tagaatgcag
     2281 tgaaaaaaat gctttatttg tgaaatttgt gatgctattg ctttatttgt aaccattata
     2341 agctgcaata aacaagttaa caacaacaat tgcattcatt ttatgtttca ggttcagggg
     2401 gaggtgtggg aggtttttta aagcaagtaa aacctctaca aatgtggtat ggctgattat
     2461 gatccggctg cctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac atgcagctcc
     2521 cggagacggt cacagcttgt ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg
     2581 cgtcagcggg tgttggcggg tgtcggggcg cagccatgag gtcgactcta gaggatcgat
     2641 ccccgccccg gacgaactaa acctgactac gacatctctg ccccttcttc gcggggcagt
     2701 gcatgtaatc ccttcagttg gttggtacaa cttgccaact gggccctgtt ccacatgtga
     2761 cacggggggg gaccaaacac aaaggggttc tctgactgta gttgacatcc ttataaatgg
     2821 atgtgcacat ttgccaacac tgagtggctt tcatcctgga gcagactttg cagtctgtgg
     2881 actgcaacac aacattgcct ttatgtgtaa ctcttggctg aagctcttac accaatgctg
     2941 ggggacatgt acctcccagg ggcccaggaa gactacggga ggctacacca acgtcaatca
     3001 gaggggcctg tgtagctacc gataagcgga ccctcaagag ggcattagca atagtgttta
     3061 taaggccccc ttgttaaccc taaacgggta gcatatgctt cccgggtagt agtatatact
     3121 atccagacta accctaattc aatagcatat gttacccaac gggaagcata tgctatcgaa
     3181 ttagggttag taaaagggtc ctaaggaaca gcgatatctc ccaccccatg agctgtcacg
     3241 gttttattta catggggtca ggattccacg agggtagtga accattttag tcacaagggc
     3301 agtggctgaa gatcaaggag cgggcagtga actctcctga atcttcgcct gcttcttcat
     3361 tctccttcgt ttagctaata gaataactgc tgagttgtga acagtaaggt gtatgtgagg
     3421 tgctcgaaaa caaggtttca ggtgacgccc ccagaataaa atttggacgg ggggttcagt
     3481 ggtggcattg tgctatgaca ccaatataac cctcacaaac cccttgggca ataaatacta
     3541 gtgtaggaat gaaacattct gaatatcttt aacaatagaa atccatgggg tggggacaag
     3601 ccgtaaagac tggatgtcca tctcacacga atttatggct atgggcaaca cataatccta
     3661 gtgcaatatg atactggggt tattaagatg tgtcccaggc agggaccaag acaggtgaac
     3721 catgttgtta cactctattt gtaacaaggg gaaagagagt ggacgccgac agcagcggac
     3781 tccactggtt gtctctaaca cccccgaaaa ttaaacgggg ctccacgcca atggggccca
     3841 taaacaaaga caagtggcca ctcttttttt tgaaattgtg gagtgggggc acgcgtcagc
     3901 ccccacacgc cgccctgcgg ttttggactg taaaataagg gtgtaataac ttggctgatt
     3961 gtaaccccgc taaccactgc ggtcaaacca cttgcccaca aaaccactaa tggcaccccg
     4021 gggaatacct gcataagtag gtgggcgggc caagataggg gcgcgattgc tgcgatctgg
     4081 aggacaaatt acacacactt gcgcctgagc gccaagcaca gggttgttgg tcctcatatt
     4141 cacgaggtcg ctgagagcac ggtgggctaa tgttgccatg ggtagcatat actacccaaa
     4201 tatctggata gcatatgcta tcctaatcta tatctgggta gcataggcta tcctaatcta
     4261 tatctgggta gcatatgcta tcctaatcta tatctgggta gtatatgcta tcctaattta
     4321 tatctgggta gcataggcta tcctaatcta tatctgggta gcatatgcta tcctaatcta
     4381 tatctgggta gtatatgcta tcctaatctg tatccgggta gcatatgcta tcctaataga
     4441 gattagggta gtatatgcta tcctaattta tatctgggta gcatatacta cccaaatatc
     4501 tggatagcat atgctatcct aatctatatc tgggtagcat atgctatcct aatctatatc
     4561 tgggtagcat aggctatcct aatctatatc tgggtagcat atgctatcct aatctatatc
     4621 tgggtagtat atgctatcct aatttatatc tgggtagcat aggctatcct aatctatatc
     4681 tgggtagcat atgctatcct aatctatatc tgggtagtat atgctatcct aatctgtatc
     4741 cgggtagcat atgctatcct catgcatata cagtcagcat atgataccca gtagtagagt
     4801 gggagtgcta tcctttgcat atgccgccac ctcccaaggg ggcgtgaatt ttcgctgctt
     4861 gtccttttcc tgctggttgc tcccattctt aggtgaattt aaggaggcca ggctaaagcc
     4921 gtcgcatgtc tgattgctca ccaggtaaat gtcgctaatg ttttccaacg cgagaaggtg
     4981 ttgagcgcgg agctgagtga cgtgacaaca tgggtatgcc caattgcccc atgttgggag
     5041 gacgaaaatg gtgacaagac agatggccag aaatacacca acagcacgca tgatgtctac
     5101 tggggattta ttctttagtg cgggggaata cacggctttt aatacgattg agggcgtctc
     5161 ctaacaagtt acatcactcc tgcccttcct caccctcatc tccatcacct ccttcatctc
     5221 cgtcatctcc gtcatcaccc tccgcggcag ccccttccac cataggtgga aaccagggag
     5281 gcaaatctac tccatcgtca aagctgcaca cagtcaccct gatattgcag gtaggagcgg
     5341 gctttgtcat aacaaggtcc ttaatcgcat ccttcaaaac ctcagcaaat atatgagttt
     5401 gtaaaaagac catgaaataa cagacaatgg actcccttag cgggccaggt tgtgggccgg
     5461 gtccaggggc cattccaaag gggagacgac tcaatggtgt aagacgacat tgtggaatag
     5521 caagggcagt tcctcgcctt aggttgtaaa gggaggtctt actacctcca tatacgaaca
     5581 caccggcgac ccaagttcct tcgtcggtag tcctttctac gtgactccta gccaggagag
     5641 ctcttaaacc ttctgcaatg ttctcaaatt tcgggttgga acctccttga ccacgatgct
     5701 ttccaaacca ccctcctttt ttgcgcctgc ctccatcacc ctgaccccgg ggtccagtgc
     5761 ttgggccttc tcctgggtca tctgcggggc cctgctctat cgctcccggg ggcacgtcag
     5821 gctcaccatc tgggccacct tcttggtggt attcaaaata atcggcttcc cctacagggt
     5881 ggaaaaatgg ccttctacct ggagggggcc tgcgcggtgg agacccggat gatgatgact
     5941 gactactggg actcctgggc ctcttttctc cacgtccacg acctctcccc ctggctcttt
     6001 cacgacttcc ccccctggct ctttcacgtc ctctaccccg gcggcctcca ctacctcctc
     6061 gaccccggcc tccactacct cctcgacccc ggcctccact gcctcctcga ccccggcctc
     6121 cacctcctgc tcctgcccct cctgctcctg cccctcctcc tgctcctgcc cctcctgctc
     6181 ctgcccctcc cgctcctgct cctgctcctg ttccaccgtg ggtccctttg cagccaatgc
     6241 aacttggacg tttttggggt ctccggacac catctctatg tcttggccct gatcctgagc
     6301 cgcccggggc tcctggtctt ccgcctcctc gtcctcgtcc tcttccccgt cctcgtccat
     6361 ggttatcacc ccctcttctt tgaggtccac tgccgccgga gccttctggt ccagatgtgt
     6421 ctcccttctc tcctaggcca tttccaggtc ctgtacctgg cccctcgtca gacatgattc
     6481 acactaaaag agatcaatag acatctttat tagacgacgc tcagtgaata cagggagtgc
     6541 agactcctgc cccctccaac agccccccca ccctcatccc cttcatggtc gctgtcagac
     6601 agatccaggt ctgaaaattc cccatcctcc gaaccatcct cgtcctcatc accaattact
     6661 cgcagcccgg aaaactcccg ctgaacatcc tcaagatttg cgtcctgagc ctcaagccag
     6721 gcctcaaatt cctcgtcccc ctttttgctg gacggtaggg atggggattc tcgggacccc
     6781 tcctcttcct cttcaaggtc accagacaga gatgctactg gggcaacgga agaaaagctg
     6841 ggtgcggcct gtgaggatca gcttatcgat gataagctgt caaacatgag aattcttgaa
     6901 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
     6961 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
     7021 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
     7081 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
     7141 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
     7201 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
     7261 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
     7321 tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac
     7381 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
     7441 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
     7501 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
     7561 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
     7621 acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg
     7681 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
     7741 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
     7801 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct
     7861 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     7921 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     7981 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     8041 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     8101 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     8161 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     8221 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc
     8281 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     8341 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     8401 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     8461 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     8521 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     8581 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     8641 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     8701 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     8761 gctggccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca
     8821 acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc
     8881 ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccccgaga tgcgccgcgt
     8941 gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt
     9001 cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag
     9061 gtgccgccct gcttcatccc cgtggcccgt tgctcgcgtt tgctggcggt gtccccggaa
     9121 gaaatatatt tgcatgtctt tagttctatg atgacacaaa ccccgcccag cgtcttgtca
     9181 ttggcgaatt cgaacacgca gatgcagtcg gggcggcgcg gtccgaggtc cacttcgcat
     9241 attaaggtga cgcgtgtggc ctcgaacacc gagcgaccct gcagcgaccc gcttaacagc
     9301 gtcaacagcg tgccgcagat cccggggggc aatgagatat gaaaaagcct gaactcaccg
     9361 cgacgtctgt cgagaagttt ctgatcgaaa agttcgacag cgtctccgac ctgatgcagc
     9421 tctcggaggg cgaagaatct cgtgctttca gcttcgatgt aggagggcgt ggatatgtcc
     9481 tgcgggtaaa tagctgcgcc gatggtttct acaaagatcg ttatgtttat cggcactttg
     9541 catcggccgc gctcccgatt ccggaagtgc ttgacattgg ggaattcagc gagagcctga
     9601 cctattgcat ctcccgccgt gcacagggtg tcacgttgca agacctgcct gaaaccgaac
     9661 tgcccgctgt tctgcagccg gtcgcggagg ccatggatgc gatcgctgcg gccgatctta
     9721 gccagacgag cgggttcggc ccattcggac cgcaaggaat cggtcaatac actacatggc
     9781 gtgatttcat atgcgcgatt gctgatcccc atgtgtatca ctggcaaact gtgatggacg
     9841 acaccgtcag tgcgtccgtc gcgcaggctc tcgatgagct gatgctttgg gccgaggact
     9901 gccccgaagt ccggcacctc gtgcacgcgg atttcggctc caacaatgtc ctgacggaca
     9961 atggccgcat aacagcggtc attgactgga gcgaggcgat gttcggggat tcccaatacg
    10021 aggtcgccaa catcttcttc tggaggccgt ggttggcttg tatggagcag cagacgcgct
    10081 acttcgagcg gaggcatccg gagcttgcag gatcgccgcg gctccgggcg tatatgctcc
    10141 gcattggtct tgaccaactc tatcagagct tggttgacgg caatttcgat gatgcagctt
    10201 gggcgcaggg tcgatgcgac gcaatcgtcc gatccggagc cgggactgtc gggcgtacac
    10261 aaatcgcccg cagaagcgcg gccgtctgga ccgatggctg tgtagaagta ctcgccgata
    10321 gtggaaaccg acgccccagc actcgtccgg atcgggagat gggggaggct aactgaaaca
    10381 cggaaggaga caataccgga aggaacccgc gctatgacgg caataaaaag acagaataaa
    10441 acgcacgggt gttgggtcgt ttgttcataa acgcggggtt cggtcccagg gctggcactc
    10501 tgtcgatacc ccaccgagac cccattgggg ccaatacgcc cgcgtttctt ccttttcccc
    10561 accccacccc ccaagttcgg gtgaaggccc agggctcgca gccaacgtcg gggcggcagg
    10621 ccctgccata gccactggcc ccgtgggtta gggacggggt cccccatggg gaatggttta
    10681 tggttcgtgg gggttattat tttgggcgtt gcgtggggtc aggtccacga ctggactgag
    10741 cagacagacc catggttttt ggatggcctg ggcatggacc gcatgtactg gcgcgacacg
    10801 aacaccgggc gtctgtggct gccaaacacc cccgaccccc aaaaaccacc gcgcggattt
    10861 ctggcgtgcc aagctagtcg accaattctc atgtttgaca gcttatcatc gcagatccgg
    10921 gcaacgttgt tgccattgct gcaggcgcag aactggtagg tatggaagat ctatacattg
    10981 aatcaatatt ggcaattagc catattagtc attggttata tagcataaat caatattggc
    11041 tattggccat tgcatacgtt gtatctatat cataatatgt acatttatat tggctcatgt
    11101 ccaatatgac cgccatgtt
//
