LOCUS       sequence-327996-       10793 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of FLAG-tagged TAPBPR with tapasin CT I261A.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 10793)
  AUTHORS   McShan AC, Devlin CA, Morozov GI, Overall SA, Moschidi D, Akella N, 
            Procko E, Sgourakis NG
  TITLE     TAPBPR promotes antigen loading on MHC-I molecules using a peptide 
            trap.
  JOURNAL   Nat Commun. 2021 May 26;12(1):3174. doi: 10.1038/s41467-021-23225-6.
  PUBMED    34039964
REFERENCE   2  (bases 1 to 10793)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2021-05-26"; volume: "12(1):3174. doi"; pages: " 
            10.1038/s41467-021-23225-6"
COMMENT     Sequence Label: pCEP4-FLAG-TAPBPR-tapasinCT I261A
FEATURES             Location/Qualifiers
     source          1..10793
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     581..605
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      633..642
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             717..740
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     polyA_signal    2224..2358
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2248..2267)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2302..2321
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(2501..2523)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(6584..6602)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6670..6774
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6775..6843,6844..7635)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6993..7012)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7806..8394
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8295..8314
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        8825..8970
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     CDS             9013..10050
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPDRE
                     MGEAN"
     primer_bind     complement(10048..10067)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    10092..10140
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt ccgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttacggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta caccaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaataa ccccgccccg ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctcgttta gtgaaccgtc
      601 agatctctag aagctgggta ccagctgcta gcgccaccat ggccgtcatg gcgccccgaa
      661 ccctcgtcct gctactctcg ggggccctgg ccctgaccca gacctgggcg ggctccgact
      721 acaaggacga tgatgacaag ggaggatcca agccccaccc agcagagggg cagtggcggg
      781 cagtggacgt ggtcctagac tgtttcctgg tgaaggacgg tgcgcaccgt ggagctctcg
      841 ccagcagtga ggacagggca agggcctccc ttgtgctgaa gcaggtgcca gtgctggacg
      901 atggctccct ggaggacttc accgatttcc aagggggcac actggcccaa gatgacccac
      961 ctattatctt tgaggcctca gtggacctgg tccagattcc ccaggccgag gccttgctcc
     1021 atgctgactg cagtgggaag gaggtgacct gtgagatctc ccgctacttt ctccagatga
     1081 cagagaccac tgttaagaca gcagcttggt tcatggccaa cgtgcaggtc tctggagggg
     1141 gacctagcat ctccttggtg atgaagactc ccagggtcgc caagaatgag gtgctctggc
     1201 acccaacgct gaacttgcca ctgagccccc aggggactgt gcgaactgca gtggagttcc
     1261 aggtgatgac acagacccaa tccctgagct tcctgctggg gtcctcagcc tccttggact
     1321 gtggcttctc catggcaccg ggcttggacc tcatcagtgt ggagtggcga ctgcagcaca
     1381 agggcagggg tcagttggtg tacagctgga ccgcagggca ggggcaggct gtgcggaagg
     1441 gcgctaccct ggagcctgca caactgggca tggccaggga tgcctccctc accctgcccg
     1501 gcctcactat acaggacgag gggacctacg cttgccagat caccacctct ctgtaccgag
     1561 ctcagcagat catccagctc aacatccaag cttcccctaa agtacgactg agcttggcaa
     1621 acgaagctct gctgcccacc ctcatctgcg acattgctgg ctattaccct ctggatgtgg
     1681 tggtgacgtg gacccgagag gagctgggtg gatccccagc ccaagtctct ggtgcctcct
     1741 tctccagcct caggcaaagc gtggcaggca cctacagcat ctcctcctct ctcaccgcag
     1801 aacctggctc tgcaggtgcc acttacacct gccaggtcac acacatctct ctggaggagc
     1861 cccttggggc cagcacccag gttgtcccac cagagcggag atcagggccc tcccttgagg
     1921 acagcgtagg ccttttcctg tctgcctttc ttctgcttgg gctcttcaag gcactgggct
     1981 gggctgctgt ctacctgtcc acctgcaagg attcaaagaa ggtacagtgc tccacctctc
     2041 tgtatctttc ccttgtcact ttatctcctc atcctatctc aaaacccatg gagggaggct
     2101 gctggtgtgg taggcagaac ctaggcttgg aattcacact gatctgggtt aaaacctggc
     2161 actatatcct aactgtagga ctctttgagc atgctactta atgactcgag gccggcaagg
     2221 ccggatccag acatgataag atacattgat gagtttggac aaaccacaac tagaatgcag
     2281 tgaaaaaaat gctttatttg tgaaatttgt gatgctattg ctttatttgt aaccattata
     2341 agctgcaata aacaagttaa caacaacaat tgcattcatt ttatgtttca ggttcagggg
     2401 gaggtgtggg aggtttttta aagcaagtaa aacctctaca aatgtggtat ggctgattat
     2461 gatccggctg cctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac atgcagctcc
     2521 cggagacggt cacagcttgt ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg
     2581 cgtcagcggg tgttggcggg tgtcggggcg cagccatgag gtcgactcta gaggatcgat
     2641 ccccgccccg gacgaactaa acctgactac gacatctctg ccccttcttc gcggggcagt
     2701 gcatgtaatc ccttcagttg gttggtacaa cttgccaact gggccctgtt ccacatgtga
     2761 cacggggggg gaccaaacac aaaggggttc tctgactgta gttgacatcc ttataaatgg
     2821 atgtgcacat ttgccaacac tgagtggctt tcatcctgga gcagactttg cagtctgtgg
     2881 actgcaacac aacattgcct ttatgtgtaa ctcttggctg aagctcttac accaatgctg
     2941 ggggacatgt acctcccagg ggcccaggaa gactacggga ggctacacca acgtcaatca
     3001 gaggggcctg tgtagctacc gataagcgga ccctcaagag ggcattagca atagtgttta
     3061 taaggccccc ttgttaaccc taaacgggta gcatatgctt cccgggtagt agtatatact
     3121 atccagacta accctaattc aatagcatat gttacccaac gggaagcata tgctatcgaa
     3181 ttagggttag taaaagggtc ctaaggaaca gcgatatctc ccaccccatg agctgtcacg
     3241 gttttattta catggggtca ggattccacg agggtagtga accattttag tcacaagggc
     3301 agtggctgaa gatcaaggag cgggcagtga actctcctga atcttcgcct gcttcttcat
     3361 tctccttcgt ttagctaata gaataactgc tgagttgtga acagtaaggt gtatgtgagg
     3421 tgctcgaaaa caaggtttca ggtgacgccc ccagaataaa atttggacgg ggggttcagt
     3481 ggtggcattg tgctatgaca ccaatataac cctcacaaac cccttgggca ataaatacta
     3541 gtgtaggaat gaaacattct gaatatcttt aacaatagaa atccatgggg tggggacaag
     3601 ccgtaaagac tggatgtcca tctcacacga atttatggct atgggcaaca cataatccta
     3661 gtgcaatatg atactggggt tattaagatg tgtcccaggc agggaccaag acaggtgaac
     3721 catgttgtta cactctattt gtaacaaggg gaaagagagt ggacgccgac agcagcggac
     3781 tccactggtt gtctctaaca cccccgaaaa ttaaacgggg ctccacgcca atggggccca
     3841 taaacaaaga caagtggcca ctcttttttt tgaaattgtg gagtgggggc acgcgtcagc
     3901 ccccacacgc cgccctgcgg ttttggactg taaaataagg gtgtaataac ttggctgatt
     3961 gtaaccccgc taaccactgc ggtcaaacca cttgcccaca aaaccactaa tggcaccccg
     4021 gggaatacct gcataagtag gtgggcgggc caagataggg gcgcgattgc tgcgatctgg
     4081 aggacaaatt acacacactt gcgcctgagc gccaagcaca gggttgttgg tcctcatatt
     4141 cacgaggtcg ctgagagcac ggtgggctaa tgttgccatg ggtagcatat actacccaaa
     4201 tatctggata gcatatgcta tcctaatcta tatctgggta gcataggcta tcctaatcta
     4261 tatctgggta gcatatgcta tcctaatcta tatctgggta gtatatgcta tcctaattta
     4321 tatctgggta gcataggcta tcctaatcta tatctgggta gcatatgcta tcctaatcta
     4381 tatctgggta gtatatgcta tcctaatctg tatccgggta gcatatgcta tcctcatgca
     4441 tatacagtca gcatatgata cccagtagta gagtgggagt gctatccttt gcatatgccg
     4501 ccacctccca agggggcgtg aattttcgct gcttgtcctt ttcctgctgg ttgctcccat
     4561 tcttaggtga atttaaggag gccaggctaa agccgtcgca tgtctgattg ctcaccaggt
     4621 aaatgtcgct aatgttttcc aacgcgagaa ggtgttgagc gcggagctga gtgacgtgac
     4681 aacatgggta tgcccaattg ccccatgttg ggaggacgaa aatggtgaca agacagatgg
     4741 ccagaaatac accaacagca cgcatgatgt ctactgggga tttattcttt agtgcggggg
     4801 aatacacggc ttttaatacg attgagggcg tctcctaaca agttacatca ctcctgccct
     4861 tcctcaccct catctccatc acctccttca tctccgtcat ctccgtcatc accctccgcg
     4921 gcagcccctt ccaccatagg tggaaaccag ggaggcaaat ctactccatc gtcaaagctg
     4981 cacacagtca ccctgatatt gcaggtagga gcgggctttg tcataacaag gtccttaatc
     5041 gcatccttca aaacctcagc aaatatatga gtttgtaaaa agaccatgaa ataacagaca
     5101 atggactccc ttagcgggcc aggttgtggg ccgggtccag gggccattcc aaaggggaga
     5161 cgactcaatg gtgtaagacg acattgtgga atagcaaggg cagttcctcg ccttaggttg
     5221 taaagggagg tcttactacc tccatatacg aacacaccgg cgacccaagt tccttcgtcg
     5281 gtagtccttt ctacgtgact cctagccagg agagctctta aaccttctgc aatgttctca
     5341 aatttcgggt tggaacctcc ttgaccacga tgctttccaa accaccctcc ttttttgcgc
     5401 ctgcctccat caccctgacc ccggggtcca gtgcttgggc cttctcctgg gtcatctgcg
     5461 gggccctgct ctatcgctcc cgggggcacg tcaggctcac catctgggcc accttcttgg
     5521 tggtattcaa aataatcggc ttcccctaca gggtggaaaa atggccttct acctggaggg
     5581 ggcctgcgcg gtggagaccc ggatgatgat gactgactac tgggactcct gggcctcttt
     5641 tctccacgtc cacgacctct ccccctggct ctttcacgac ttccccccct ggctctttca
     5701 cgtcctctac cccggcggcc tccactacct cctcgacccc ggcctccact acctcctcga
     5761 ccccggcctc cactgcctcc tcgaccccgg cctccacctc ctgctcctgc ccctcctgct
     5821 cctgcccctc ctcctgctcc tgcccctcct gctcctgccc ctcccgctcc tgctcctgct
     5881 cctgttccac cgtgggtccc tttgcagcca atgcaacttg gacgtttttg gggtctccgg
     5941 acaccatctc tatgtcttgg ccctgatcct gagccgcccg gggctcctgg tcttccgcct
     6001 cctcgtcctc gtcctcttcc ccgtcctcgt ccatggttat caccccctct tctttgaggt
     6061 ccactgccgc cggagccttc tggtccagat gtgtctccct tctctcctag gccatttcca
     6121 ggtcctgtac ctggcccctc gtcagacatg attcacacta aaagagatca atagacatct
     6181 ttattagacg acgctcagtg aatacaggga gtgcagactc ctgccccctc caacagcccc
     6241 cccaccctca tccccttcat ggtcgctgtc agacagatcc aggtctgaaa attccccatc
     6301 ctccgaacca tcctcgtcct catcaccaat tactcgcagc ccggaaaact cccgctgaac
     6361 atcctcaaga tttgcgtcct gagcctcaag ccaggcctca aattcctcgt cccccttttt
     6421 gctggacggt agggatgggg attctcggga cccctcctct tcctcttcaa ggtcaccaga
     6481 cagagatgct actggggcaa cggaagaaaa gctgggtgcg gcctgtgagg atcagcttat
     6541 cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta
     6601 tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg
     6661 ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg
     6721 ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt
     6781 attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt
     6841 gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg
     6901 ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa
     6961 cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt
     7021 gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag
     7081 tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt
     7141 gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga
     7201 ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt
     7261 tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca
     7321 gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg
     7381 caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc
     7441 cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt
     7501 atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg
     7561 gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg
     7621 attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa
     7681 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa
     7741 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
     7801 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
     7861 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
     7921 ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac
     7981 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
     8041 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
     8101 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
     8161 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
     8221 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
     8281 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
     8341 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
     8401 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttgaagctg tccctgatgg
     8461 tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa
     8521 gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg
     8581 tagcccagcg cgtcggcccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga
     8641 tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg
     8701 ctccaattct tggagtggtg aatccgttag cgaggtgccg ccctgcttca tccccgtggc
     8761 ccgttgctcg cgtttgctgg cggtgtcccc ggaagaaata tatttgcatg tctttagttc
     8821 tatgatgaca caaaccccgc ccagcgtctt gtcattggcg aattcgaaca cgcagatgca
     8881 gtcggggcgg cgcggtccga ggtccacttc gcatattaag gtgacgcgtg tggcctcgaa
     8941 caccgagcga ccctgcagcg acccgcttaa cagcgtcaac agcgtgccgc agatcccggg
     9001 gggcaatgag atatgaaaaa gcctgaactc accgcgacgt ctgtcgagaa gtttctgatc
     9061 gaaaagttcg acagcgtctc cgacctgatg cagctctcgg agggcgaaga atctcgtgct
     9121 ttcagcttcg atgtaggagg gcgtggatat gtcctgcggg taaatagctg cgccgatggt
     9181 ttctacaaag atcgttatgt ttatcggcac tttgcatcgg ccgcgctccc gattccggaa
     9241 gtgcttgaca ttggggaatt cagcgagagc ctgacctatt gcatctcccg ccgtgcacag
     9301 ggtgtcacgt tgcaagacct gcctgaaacc gaactgcccg ctgttctgca gccggtcgcg
     9361 gaggccatgg atgcgatcgc tgcggccgat cttagccaga cgagcgggtt cggcccattc
     9421 ggaccgcaag gaatcggtca atacactaca tggcgtgatt tcatatgcgc gattgctgat
     9481 ccccatgtgt atcactggca aactgtgatg gacgacaccg tcagtgcgtc cgtcgcgcag
     9541 gctctcgatg agctgatgct ttgggccgag gactgccccg aagtccggca cctcgtgcac
     9601 gcggatttcg gctccaacaa tgtcctgacg gacaatggcc gcataacagc ggtcattgac
     9661 tggagcgagg cgatgttcgg ggattcccaa tacgaggtcg ccaacatctt cttctggagg
     9721 ccgtggttgg cttgtatgga gcagcagacg cgctacttcg agcggaggca tccggagctt
     9781 gcaggatcgc cgcggctccg ggcgtatatg ctccgcattg gtcttgacca actctatcag
     9841 agcttggttg acggcaattt cgatgatgca gcttgggcgc agggtcgatg cgacgcaatc
     9901 gtccgatccg gagccgggac tgtcgggcgt acacaaatcg cccgcagaag cgcggccgtc
     9961 tggaccgatg gctgtgtaga agtactcgcc gatagtggaa accgacgccc cagcactcgt
    10021 ccggatcggg agatggggga ggctaactga aacacggaag gagacaatac cggaaggaac
    10081 ccgcgctatg acggcaataa aaagacagaa taaaacgcac gggtgttggg tcgtttgttc
    10141 ataaacgcgg ggttcggtcc cagggctggc actctgtcga taccccaccg agaccccatt
    10201 ggggccaata cgcccgcgtt tcttcctttt ccccacccca ccccccaagt tcgggtgaag
    10261 gcccagggct cgcagccaac gtcggggcgg caggccctgc catagccact ggccccgtgg
    10321 gttagggacg gggtccccca tggggaatgg tttatggttc gtgggggtta ttattttggg
    10381 cgttgcgtgg ggtcaggtcc acgactggac tgagcagaca gacccatggt ttttggatgg
    10441 cctgggcatg gaccgcatgt actggcgcga cacgaacacc gggcgtctgt ggctgccaaa
    10501 cacccccgac ccccaaaaac caccgcgcgg atttctggcg tgccaagcta gtcgaccaat
    10561 tctcatgttt gacagcttat catcgcagat ccgggcaacg ttgttgccat tgctgcaggc
    10621 gcagaactgg taggtatgga agatctatac attgaatcaa tattggcaat tagccatatt
    10681 agtcattggt tatatagcat aaatcaatat tggctattgg ccattgcata cgttgtatct
    10741 atatcataat atgtacattt atattggctc atgtccaata tgaccgccat gtt
//
