LOCUS       sequence-327998-       11120 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of FLAG-tagged TAPBPR with tapasin CT G212A.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 11120)
  AUTHORS   McShan AC, Devlin CA, Morozov GI, Overall SA, Moschidi D, Akella N, 
            Procko E, Sgourakis NG
  TITLE     TAPBPR promotes antigen loading on MHC-I molecules using a peptide 
            trap.
  JOURNAL   Nat Commun. 2021 May 26;12(1):3174. doi: 10.1038/s41467-021-23225-6.
  PUBMED    34039964
REFERENCE   2  (bases 1 to 11120)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2021-05-26"; volume: "12(1):3174. doi"; pages: " 
            10.1038/s41467-021-23225-6"
COMMENT     Sequence Label: pCEP4-FLAG-TAPBPR-tapasinCT G212A
FEATURES             Location/Qualifiers
     source          1..11120
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     581..605
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      633..642
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             717..740
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     polyA_signal    2224..2358
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2248..2267)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2302..2321
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(2501..2523)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      3083..4872
                     /label=oriP
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
     primer_bind     complement(6911..6929)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6997..7101
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7102..7170,7171..7962)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7320..7339)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8133..8721
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8622..8641
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        9152..9297
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     CDS             9340..10377
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPDRE
                     MGEAN"
     primer_bind     complement(10375..10394)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    10419..10467
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt ccgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttacggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta caccaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaataa ccccgccccg ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctcgttta gtgaaccgtc
      601 agatctctag aagctgggta ccagctgcta gcgccaccat ggccgtcatg gcgccccgaa
      661 ccctcgtcct gctactctcg ggggccctgg ccctgaccca gacctgggcg ggctccgact
      721 acaaggacga tgatgacaag ggaggatcca agccccaccc agcagagggg cagtggcggg
      781 cagtggacgt ggtcctagac tgtttcctgg tgaaggacgg tgcgcaccgt ggagctctcg
      841 ccagcagtga ggacagggca agggcctccc ttgtgctgaa gcaggtgcca gtgctggacg
      901 atggctccct ggaggacttc accgatttcc aagggggcac actggcccaa gatgacccac
      961 ctattatctt tgaggcctca gtggacctgg tccagattcc ccaggccgag gccttgctcc
     1021 atgctgactg cagtgggaag gaggtgacct gtgagatctc ccgctacttt ctccagatga
     1081 cagagaccac tgttaagaca gcagcttggt tcatggccaa cgtgcaggtc tctggagggg
     1141 gacctagcat ctccttggtg atgaagactc ccagggtcgc caagaatgag gtgctctggc
     1201 acccaacgct gaacttgcca ctgagccccc aggggactgt gcgaactgca gtggagttcc
     1261 aggtgatgac acagacccaa tccctgagct tcctgctggg gtcctcagcc tccttggact
     1321 gtggcttctc catggcaccg ggcttggacc tcatcagtgt ggagtggcga ctgcagcaca
     1381 aggccagggg tcagttggtg tacagctgga ccgcagggca ggggcaggct gtgcggaagg
     1441 gcgctaccct ggagcctgca caactgggca tggccaggga tgcctccctc accctgcccg
     1501 gcctcactat acaggacgag gggacctaca tttgccagat caccacctct ctgtaccgag
     1561 ctcagcagat catccagctc aacatccaag cttcccctaa agtacgactg agcttggcaa
     1621 acgaagctct gctgcccacc ctcatctgcg acattgctgg ctattaccct ctggatgtgg
     1681 tggtgacgtg gacccgagag gagctgggtg gatccccagc ccaagtctct ggtgcctcct
     1741 tctccagcct caggcaaagc gtggcaggca cctacagcat ctcctcctct ctcaccgcag
     1801 aacctggctc tgcaggtgcc acttacacct gccaggtcac acacatctct ctggaggagc
     1861 cccttggggc cagcacccag gttgtcccac cagagcggag atcagggccc tcccttgagg
     1921 acagcgtagg ccttttcctg tctgcctttc ttctgcttgg gctcttcaag gcactgggct
     1981 gggctgctgt ctacctgtcc acctgcaagg attcaaagaa ggtacagtgc tccacctctc
     2041 tgtatctttc ccttgtcact ttatctcctc atcctatctc aaaacccatg gagggaggct
     2101 gctggtgtgg taggcagaac ctaggcttgg aattcacact gatctgggtt aaaacctggc
     2161 actatatcct aactgtagga ctctttgagc atgctactta atgactcgag gccggcaagg
     2221 ccggatccag acatgataag atacattgat gagtttggac aaaccacaac tagaatgcag
     2281 tgaaaaaaat gctttatttg tgaaatttgt gatgctattg ctttatttgt aaccattata
     2341 agctgcaata aacaagttaa caacaacaat tgcattcatt ttatgtttca ggttcagggg
     2401 gaggtgtggg aggtttttta aagcaagtaa aacctctaca aatgtggtat ggctgattat
     2461 gatccggctg cctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac atgcagctcc
     2521 cggagacggt cacagcttgt ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg
     2581 cgtcagcggg tgttggcggg tgtcggggcg cagccatgag gtcgactcta gaggatcgat
     2641 ccccgccccg gacgaactaa acctgactac gacatctctg ccccttcttc gcggggcagt
     2701 gcatgtaatc ccttcagttg gttggtacaa cttgccaact gggccctgtt ccacatgtga
     2761 cacggggggg gaccaaacac aaaggggttc tctgactgta gttgacatcc ttataaatgg
     2821 atgtgcacat ttgccaacac tgagtggctt tcatcctgga gcagactttg cagtctgtgg
     2881 actgcaacac aacattgcct ttatgtgtaa ctcttggctg aagctcttac accaatgctg
     2941 ggggacatgt acctcccagg ggcccaggaa gactacggga ggctacacca acgtcaatca
     3001 gaggggcctg tgtagctacc gataagcgga ccctcaagag ggcattagca atagtgttta
     3061 taaggccccc ttgttaaccc taaacgggta gcatatgctt cccgggtagt agtatatact
     3121 atccagacta accctaattc aatagcatat gttacccaac gggaagcata tgctatcgaa
     3181 ttagggttag taaaagggtc ctaaggaaca gcgatatctc ccaccccatg agctgtcacg
     3241 gttttattta catggggtca ggattccacg agggtagtga accattttag tcacaagggc
     3301 agtggctgaa gatcaaggag cgggcagtga actctcctga atcttcgcct gcttcttcat
     3361 tctccttcgt ttagctaata gaataactgc tgagttgtga acagtaaggt gtatgtgagg
     3421 tgctcgaaaa caaggtttca ggtgacgccc ccagaataaa atttggacgg ggggttcagt
     3481 ggtggcattg tgctatgaca ccaatataac cctcacaaac cccttgggca ataaatacta
     3541 gtgtaggaat gaaacattct gaatatcttt aacaatagaa atccatgggg tggggacaag
     3601 ccgtaaagac tggatgtcca tctcacacga atttatggct atgggcaaca cataatccta
     3661 gtgcaatatg atactggggt tattaagatg tgtcccaggc agggaccaag acaggtgaac
     3721 catgttgtta cactctattt gtaacaaggg gaaagagagt ggacgccgac agcagcggac
     3781 tccactggtt gtctctaaca cccccgaaaa ttaaacgggg ctccacgcca atggggccca
     3841 taaacaaaga caagtggcca ctcttttttt tgaaattgtg gagtgggggc acgcgtcagc
     3901 ccccacacgc cgccctgcgg ttttggactg taaaataagg gtgtaataac ttggctgatt
     3961 gtaaccccgc taaccactgc ggtcaaacca cttgcccaca aaaccactaa tggcaccccg
     4021 gggaatacct gcataagtag gtgggcgggc caagataggg gcgcgattgc tgcgatctgg
     4081 aggacaaatt acacacactt gcgcctgagc gccaagcaca gggttgttgg tcctcatatt
     4141 cacgaggtcg ctgagagcac ggtgggctaa tgttgccatg ggtagcatat actacccaaa
     4201 tatctggata gcatatgcta tcctaatcta tatctgggta gcataggcta tcctaatcta
     4261 tatctgggta gcatatgcta tcctaatcta tatctgggta gtatatgcta tcctaattta
     4321 tatctgggta gcataggcta tcctaatcta tatctgggta gcatatgcta tcctaatcta
     4381 tatctgggta gtatatgcta tcctaatctg tatccgggta gcatatgcta tcctaataga
     4441 gattagggta gtatatgcta tcctaattta tatctgggta gcatatacta cccaaatatc
     4501 tggatagcat atgctatcct aatctatatc tgggtagcat atgctatcct aatctatatc
     4561 tgggtagcat aggctatcct aatctatatc tgggtagcat atgctatcct aatctatatc
     4621 tgggtagtat atgctatcct aatttatatc tgggtagcat aggctatcct aatctatatc
     4681 tgggtagcat atgctatcct aatctatatc tgggtagtat atgctatcct aatctgtatc
     4741 cgggtagcat atgctatcct catgcatata cagtcagcat atgataccca gtagtagagt
     4801 gggagtgcta tcctttgcat atgccgccac ctcccaaggg ggcgtgaatt ttcgctgctt
     4861 gtccttttcc tgctggttgc tcccattctt aggtgaattt aaggaggcca ggctaaagcc
     4921 gtcgcatgtc tgattgctca ccaggtaaat gtcgctaatg ttttccaacg cgagaaggtg
     4981 ttgagcgcgg agctgagtga cgtgacaaca tgggtatgcc caattgcccc atgttgggag
     5041 gacgaaaatg gtgacaagac agatggccag aaatacacca acagcacgca tgatgtctac
     5101 tggggattta ttctttagtg cgggggaata cacggctttt aatacgattg agggcgtctc
     5161 ctaacaagtt acatcactcc tgcccttcct caccctcatc tccatcacct ccttcatctc
     5221 cgtcatctcc gtcatcaccc tccgcggcag ccccttccac cataggtgga aaccagggag
     5281 gcaaatctac tccatcgtca aagctgcaca cagtcaccct gatattgcag gtaggagcgg
     5341 gctttgtcat aacaaggtcc ttaatcgcat ccttcaaaac ctcagcaaat atatgagttt
     5401 gtaaaaagac catgaaataa cagacaatgg actcccttag cgggccaggt tgtgggccgg
     5461 gtccaggggc cattccaaag gggagacgac tcaatggtgt aagacgacat tgtggaatag
     5521 caagggcagt tcctcgcctt aggttgtaaa gggaggtctt actacctcca tatacgaaca
     5581 caccggcgac ccaagttcct tcgtcggtag tcctttctac gtgactccta gccaggagag
     5641 ctcttaaacc ttctgcaatg ttctcaaatt tcgggttgga acctccttga ccacgatgct
     5701 ttccaaacca ccctcctttt ttgcgcctgc ctccatcacc ctgaccccgg ggtccagtgc
     5761 ttgggccttc tcctgggtca tctgcggggc cctgctctat cgctcccggg ggcacgtcag
     5821 gctcaccatc tgggccacct tcttggtggt attcaaaata atcggcttcc cctacagggt
     5881 ggaaaaatgg ccttctacct ggagggggcc tgcgcggtgg agacccggat gatgatgact
     5941 gactactggg actcctgggc ctcttttctc cacgtccacg acctctcccc ctggctcttt
     6001 cacgacttcc ccccctggct ctttcacgtc ctctaccccg gcggcctcca ctacctcctc
     6061 gaccccggcc tccactacct cctcgacccc ggcctccact gcctcctcga ccccggcctc
     6121 cacctcctgc tcctgccncc tcctgctcct gcccctcctc ctgctcctgc ccctcctgct
     6181 cctgcccctc ccgctcctgc tcctgctcct gttccaccgt gggtcccttt gcagccaatg
     6241 caacttggac gtttttgggg tctccggaca ccatctctat gtcttggccc tgatcctgag
     6301 ccgcccgggg ctcctggtct tccgcctcct cgtcctcgtc ctcttccccg tcctcgtcca
     6361 tggttatcac cccctcttct ttgaggtcca ctgccgccgg agccttctgg tccagatgtg
     6421 tctcccttct ctcctaggcc atttccaggt cctgtacctg gcccctcgtc agacatgatt
     6481 cacactaaaa gagatcaata gacatcttta ttagacgacg ctcagtgaat acagggagtg
     6541 cagactcctg ccccctccaa cagccccccc accctcatcc ccttcatggt cgctgtcaga
     6601 cagatccagg tctgaaaatt ccccatcctc cgaaccatcc tcgtcctcat caccaattac
     6661 tcgcagcccg gaaaactccc gctgaacatc ctcaagattt gcgtcctgag cctcaagcca
     6721 ggcctcaaat tcctcgtccc cctttttgct ggacggtagg gatggggatt ctcgggaccc
     6781 ctcctcttcc tcttcaaggt caccagacag agatgctact ggggcaacgg aagaaaagct
     6841 gggtgcggcc tgtgaggatc agcttatcga tgataagctg tcaaacatga gaattcttga
     6901 agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt
     6961 tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt
     7021 ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa
     7081 taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt
     7141 tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat
     7201 gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag
     7261 atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg
     7321 ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata
     7381 cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat
     7441 ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc
     7501 aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg
     7561 ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac
     7621 gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact
     7681 ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa
     7741 gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct
     7801 ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc
     7861 tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga
     7921 cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac
     7981 tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag
     8041 atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg
     8101 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc
     8161 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag
     8221 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc
     8281 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac
     8341 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc
     8401 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt
     8461 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt
     8521 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc
     8581 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt
     8641 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca
     8701 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt
     8761 tgctggcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc
     8821 aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag
     8881 cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccccgag atgcgccgcg
     8941 tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat
     9001 tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga
     9061 ggtgccgccc tgcttcatcc ccgtggcccg ttgctcgcgt ttgctggcgg tgtccccgga
     9121 agaaatatat ttgcatgtct ttagttctat gatgacacaa accccgccca gcgtcttgtc
     9181 attggcgaat tcgaacacgc agatgcagtc ggggcggcgc ggtccgaggt ccacttcgca
     9241 tattaaggtg acgcgtgtgg cctcgaacac cgagcgaccc tgcagcgacc cgcttaacag
     9301 cgtcaacagc gtgccgcaga tcccgggggg caatgagata tgaaaaagcc tgaactcacc
     9361 gcgacgtctg tcgagaagtt tctgatcgaa aagttcgaca gcgtctccga cctgatgcag
     9421 ctctcggagg gcgaagaatc tcgtgctttc agcttcgatg taggagggcg tggatatgtc
     9481 ctgcgggtaa atagctgcgc cgatggtttc tacaaagatc gttatgttta tcggcacttt
     9541 gcatcggccg cgctcccgat tccggaagtg cttgacattg gggaattcag cgagagcctg
     9601 acctattgca tctcccgccg tgcacagggt gtcacgttgc aagacctgcc tgaaaccgaa
     9661 ctgcccgctg ttctgcagcc ggtcgcggag gccatggatg cgatcgctgc ggccgatctt
     9721 agccagacga gcgggttcgg cccattcgga ccgcaaggaa tcggtcaata cactacatgg
     9781 cgtgatttca tatgcgcgat tgctgatccc catgtgtatc actggcaaac tgtgatggac
     9841 gacaccgtca gtgcgtccgt cgcgcaggct ctcgatgagc tgatgctttg ggccgaggac
     9901 tgccccgaag tccggcacct cgtgcacgcg gatttcggct ccaacaatgt cctgacggac
     9961 aatggccgca taacagcggt cattgactgg agcgaggcga tgttcgggga ttcccaatac
    10021 gaggtcgcca acatcttctt ctggaggccg tggttggctt gtatggagca gcagacgcgc
    10081 tacttcgagc ggaggcatcc ggagcttgca ggatcgccgc ggctccgggc gtatatgctc
    10141 cgcattggtc ttgaccaact ctatcagagc ttggttgacg gcaatttcga tgatgcagct
    10201 tgggcgcagg gtcgatgcga cgcaatcgtc cgatccggag ccgggactgt cgggcgtaca
    10261 caaatcgccc gcagaagcgc ggccgtctgg accgatggct gtgtagaagt actcgccgat
    10321 agtggaaacc gacgccccag cactcgtccg gatcgggaga tgggggaggc taactgaaac
    10381 acggaaggag acaataccgg aaggaacccg cgctatgacg gcaataaaaa gacagaataa
    10441 aacgcacggg tgttgggtcg tttgttcata aacgcggggt tcggtcccag ggctggcact
    10501 ctgtcgatac cccaccgaga ccccattggg gccaatacgc ccgcgtttct tccttttccc
    10561 caccccaccc cccaagttcg ggtgaaggcc cagggctcgc agccaacgtc ggggcggcag
    10621 gccctgccat agccactggc cccgtgggtt agggacgggg tcccccatgg ggaatggttt
    10681 atggttcgtg ggggttatta ttttgggcgt tgcgtggggt caggtccacg actggactga
    10741 gcagacagac ccatggtttt tggatggcct gggcatggac cgcatgtact ggcgcgacac
    10801 gaacaccggg cgtctgtggc tgccaaacac ccccgacccc caaaaaccac cgcgcggatt
    10861 tctggcgtgc caagctagtc gaccaattct catgtttgac agcttatcat cgcagatccg
    10921 ggcaacgttg ttgccattgc tgcaggcgca gaactggtag gtatggaaga tctatacatt
    10981 gaatcaatat tggcaattag ccatattagt cattggttat atagcataaa tcaatattgg
    11041 ctattggcca ttgcatacgt tgtatctata tcataatatg tacatttata ttggctcatg
    11101 tccaatatga ccgccatgtt
//
