LOCUS       sequence-328002-        8669 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of candidate TCR #10 TCRalpha cDNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8669)
  AUTHORS   Lee MN, Meyerson M
  TITLE     Antigen identification for HLA class I- and HLA class II-restricted 
            T cell receptors using cytokine-capturing antigen-presenting cells.
  JOURNAL   Sci Immunol. 2021 Jan 22;6(55). pii: 6/55/eabf4001. doi: 
            10.1126/sciimmunol.abf4001.
  PUBMED    33483338
REFERENCE   2  (bases 1 to 8669)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci 
            Immunol."; date: "2021-01-22"; volume: "6(55). pii: 6/55/eabf4001. 
            doi"; pages: " 10.1126/sciimmunol.abf4001"
COMMENT     Sequence Label: CMVTCRa_candidate10
FEATURES             Location/Qualifiers
     source          1..8669
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        65..368
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        369..572
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     523..543
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      608..617
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    1480..2068
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(1533..1553)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(1951..1962)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             2140..2373
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    2451..2572
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2488..2507)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2542..2561
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      2612..2747
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(2767..2786)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2768..2786)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2796..2813)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2796..2812)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2805..2827)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      2954..3409
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3041..3060)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3251..3272
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        3435..3539
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3540..3608,3609..4400)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3758..3777)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4571..5159
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5060..5079
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5313..5330
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    5447..5468
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(5483..5488,5489..5506,5507..5513)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5521..5537
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5526..5548
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     5545..5561
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5545..5561
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     5580..5600
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        5582..5600
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        5628..5854
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             5855..6035
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    6082..6207
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    6700..6933
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             7118..7162
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        7337..7837
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     7756..7774
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             7859..8458
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(7859..7878)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     8355..8375
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    8500..8617
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 cccggggtta ttaatagtaa tcaattacgg ggtcattagt tcatagccca tatatggagt
       61 tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc
      121 cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac
      181 gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata
      241 tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc
      301 agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta
      361 ttaccatggt gatgcggttt tggcagtaca tcaatgggcg tggatagcgg tttgactcac
      421 ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttgg caccaaaatc
      481 aacgggactt tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc
      541 gtgtacggtg ggaggtctat ataagcagag ctctctggct aactgtcggg atcaacaagt
      601 ttgtgccgcc accatggaga ccctcttggg cctgcttatc ctttggctgc agctgcaatg
      661 ggtgagcagc aaacaggagg tgacgcagat tcctgcagct ctgagtgtcc cagaaggaga
      721 aaacttggtt ctcaactgca gtttcactga tagcgctatt tacaacctcc agtggtttag
      781 gcaggaccct gggaaaggtc tcacatctct gttgcttatt cagtcaagtc agagagagca
      841 aacaagtgga agacttaatg cctcgctgga taaatcatca ggacgtagta ctttatacat
      901 tgcagcttct cagcctggtg actcagccac ctacctctgt gctgtgagga gaggcaccga
      961 caagctcatc tttgggactg ggaccagatt acaagtcttt ccaaatatcc agaaccctga
     1021 ccctgccgtg taccagctga gagactctaa atccagtgac aagtctgtct gcctattcac
     1081 cgattttgat tctcaaacaa atgtgtcaca aagtaaggat tctgatgtgt atatcacaga
     1141 caaatgtgtg ctagacatga ggtctatgga ttttaaatct aattcagcag tagcctggag
     1201 caacaaatct gactttgcat gtgcaaacgc cttcaacaac agcattattc cagaagacac
     1261 cttcttcccc agcccagaaa gttcctgtga tgtcaagctg gtcgagaaaa gctttgaaac
     1321 agatacgaac ctaaactttc aaaacctgtc agtgattggg ttccgaatcc tcctcctgaa
     1381 agtggccggg tttaatctgc tcatgacgct gcggctgtgg tccagctaga aagtggttta
     1441 gtaatgagct agcgctaacc ggtggcgcgt taagtcgaca atcaacctct ggattacaaa
     1501 atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac
     1561 gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc
     1621 ttgtataaat cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt
     1681 ggcgtggtgt gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc
     1741 tgtcagctcc tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc
     1801 gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg
     1861 gtgttgtcgg ggaaatcatc gtcctttcct tggctgctcg cctgtgttgc cacctggatt
     1921 ctgcgcggga cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc
     1981 cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt
     2041 cggatctccc tttgggccgc ctccccgcgt cgactttaag accaatgact tacaaggcag
     2101 ctgtagatct tagccacttt ttaaaagaaa aggggggact ggaagggcta attcactccc
     2161 aacgaagaca agatctgctt tttgcttgta ctgggtctct ctggttagac cagatctgag
     2221 cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt
     2281 gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca
     2341 gaccctttta gtcagtgtgg aaaatctcta gcagtacgta tagtagttca tgtcatctta
     2401 ttattcagta tttataactt gcaaagaaat gaatatcaga gagtgagagg aacttgttta
     2461 ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat
     2521 ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct
     2581 ggctctagct atcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc
     2641 ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc
     2701 ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag ggacgtaccc
     2761 aattcgccct atagtgagtc gtattacgcg cgctcactgg ccgtcgtttt acaacgtcgt
     2821 gactgggaaa accctggcgt tacccaactt aatcgccttg cagcacatcc ccctttcgcc
     2881 agctggcgta atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg
     2941 aatggcgaat gggacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg
     3001 cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct
     3061 tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta
     3121 gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt
     3181 tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg
     3241 ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat
     3301 tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt
     3361 taacaaaaat ttaacgcgaa ttttaacaaa atattaacgc ttacaattta ggtggcactt
     3421 ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt
     3481 atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta
     3541 tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg
     3601 tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac
     3661 gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg
     3721 aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc
     3781 gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg
     3841 ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat
     3901 gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg
     3961 gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg
     4021 atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc
     4081 ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt
     4141 cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct
     4201 cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc
     4261 gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca
     4321 cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct
     4381 cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt
     4441 taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga
     4501 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca
     4561 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
     4621 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
     4681 taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag
     4741 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
     4801 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
     4861 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
     4921 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc
     4981 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
     5041 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
     5101 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
     5161 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
     5221 tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg
     5281 ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag
     5341 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
     5401 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
     5461 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
     5521 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagcgcg
     5581 caattaaccc tcactaaagg gaacaaaagc tggagctgca agcttaatgt agtcttatgc
     5641 aatactcttg tagtcttgca acatggtaac gatgagttag caacatgcct tacaaggaga
     5701 gaaaaagcac cgtgcatgcc gattggtgga agtaaggtgg tacgatcgtg ccttattagg
     5761 aaggcaacag acgggtctga catggattgg acgaaccact gaattgccgc attgcagaga
     5821 tattgtattt aagtgcctag ctcgatacat aaacgggtct ctctggttag accagatctg
     5881 agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc
     5941 ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct
     6001 cagacccttt tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa
     6061 gcgaaaggga aaccagagga gctctctcga cgcaggactc ggcttgctga agcgcgcacg
     6121 gcaagaggcg aggggcggcg actggtgagt acgccaaaaa ttttgactag cggaggctag
     6181 aaggagagag atgggtgcga gagcgtcagt attaagcggg ggagaattag atcgcgatgg
     6241 gaaaaaattc ggttaaggcc agggggaaag aaaaaatata aattaaaaca tatagtatgg
     6301 gcaagcaggg agctagaacg attcgcagtt aatcctggcc tgttagaaac atcagaaggc
     6361 tgtagacaaa tactgggaca gctacaacca tcccttcaga caggatcaga agaacttaga
     6421 tcattatata atacagtagc aaccctctat tgtgtgcatc aaaggataga gataaaagac
     6481 accaaggaag ctttagacaa gatagaggaa gagcaaaaca aaagtaagac caccgcacag
     6541 caagcggccg ctgatcttca gacctggagg aggagatatg agggacaatt ggagaagtga
     6601 attatataaa tataaagtag taaaaattga accattagga gtagcaccca ccaaggcaaa
     6661 gagaagagtg gtgcagagag aaaaaagagc agtgggaata ggagctttgt tccttgggtt
     6721 cttgggagca gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg tacaggccag
     6781 acaattattg tctggtatag tgcagcagca gaacaatttg ctgagggcta ttgaggcgca
     6841 acagcatctg ttgcaactca cagtctgggg catcaagcag ctccaggcaa gaatcctggc
     6901 tgtggaaaga tacctaaagg atcaacagct cctggggatt tggggttgct ctggaaaact
     6961 catttgcacc actgctgtgc cttggaatgc tagttggagt aataaatctc tggaacagat
     7021 ttggaatcac acgacctgga tggagtggga cagagaaatt aacaattaca caagcttaat
     7081 acactcctta attgaagaat cgcaaaacca gcaagaaaag aatgaacaag aattattgga
     7141 attagataaa tgggcaagtt tgtggaattg gtttaacata acaaattggc tgtggtatat
     7201 aaaattattc ataatgatag taggaggctt ggtaggttta agaatagttt ttgctgtact
     7261 ttctatagtg aatagagtta ggcagggata ttcaccatta tcgtttcaga cccacctccc
     7321 aaccccgagg ggacccttgc gccttttcca aggcagccct gggtttgcgc agggacgcgg
     7381 ctgctctggg cgtggttccg ggaaacgcag cggcgccgac cctgggtctc gcacattctt
     7441 cacgtccgtt cgcagcgtca cccggatctt cgccgctacc cttgtgggcc ccccggcgac
     7501 gcttcctgct ccgcccctaa gtcgggaagg ttccttgcgg ttcgcggcgt gccggacgtg
     7561 acaaacggaa gccgcacgtc tcactagtac cctcgcagac ggacagcgcc agggagcaat
     7621 ggcagcgcgc cgaccgcgat gggctgtggc caatagcggc tgctcagcag ggcgcgccga
     7681 gagcagcggc cgggaagggg cggtgcggga ggcggggtgt ggggcggtag tgtgggccct
     7741 gttcctgccc gcgcggtgtt ccgcattctg caagcctccg gagcgcacgt cggcagtcgg
     7801 ctccctcgtt gaccgaatca ccgacctctc tccccagggg gtaccaccgg agcttaccat
     7861 gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac gacgtcccca gggccgtacg
     7921 caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg atccggaccg
     7981 ccacatcgag cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg ggctcgacat
     8041 cggcaaggtg tgggtcgcgg acgacggcgc cgccgtggcg gtctggacca cgccggagag
     8101 cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc atggccgagt tgagcggttc
     8161 ccggctggcc gcgcagcaac agatggaagg cctcctggcg ccgcaccggc ccaaggagcc
     8221 cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac cagggcaagg gtctgggcag
     8281 cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg ccttcctgga
     8341 gacctccgcg ccccgcaacc tccccttcta cgagcggctc ggcttcaccg tcaccgccga
     8401 cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg gtgcctgaga
     8461 attctagatc ttgagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat
     8521 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
     8581 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag
     8641 agatccactt tggcgccggc tcgaggggg
//
