LOCUS       sequence-328005-        8670 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of candidate TCR #2 TCRalpha cDNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8670)
  AUTHORS   Lee MN, Meyerson M
  TITLE     Antigen identification for HLA class I- and HLA class II-restricted 
            T cell receptors using cytokine-capturing antigen-presenting cells.
  JOURNAL   Sci Immunol. 2021 Jan 22;6(55). pii: 6/55/eabf4001. doi: 
            10.1126/sciimmunol.abf4001.
  PUBMED    33483338
REFERENCE   2  (bases 1 to 8670)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci 
            Immunol."; date: "2021-01-22"; volume: "6(55). pii: 6/55/eabf4001. 
            doi"; pages: " 10.1126/sciimmunol.abf4001"
COMMENT     Sequence Label: CMVTCRa_candidate2
FEATURES             Location/Qualifiers
     source          1..8670
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        65..368
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        369..572
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     523..543
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     misc_feature    1481..2069
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(1534..1554)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(1952..1963)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             2141..2374
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    2452..2573
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2489..2508)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2543..2562
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      2613..2748
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(2768..2787)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2769..2787)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2797..2814)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2797..2813)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2806..2828)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      2955..3410
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3042..3061)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3252..3273
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        3436..3540
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3541..3609,3610..4401)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3759..3778)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4572..5160
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5061..5080
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5314..5331
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    5448..5469
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(5484..5489,5490..5507,5508..5514)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5522..5538
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5527..5549
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     5546..5562
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5546..5562
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     5581..5601
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        5583..5601
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        5629..5855
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             5856..6036
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    6083..6208
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    6701..6934
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             7119..7163
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        7338..7838
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     7757..7775
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             7860..8459
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(7860..7879)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     8356..8376
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    8501..8618
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 cccggggtta ttaatagtaa tcaattacgg ggtcattagt tcatagccca tatatggagt
       61 tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc
      121 cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac
      181 gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata
      241 tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc
      301 agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta
      361 ttaccatggt gatgcggttt tggcagtaca tcaatgggcg tggatagcgg tttgactcac
      421 ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttgg caccaaaatc
      481 aacgggactt tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc
      541 gtgtacggtg ggaggtctat ataagcagag ctctctggct aactgtcggg atcaacaagt
      601 ttgccgccac catgaactat tctccaggct tagtatctct gatactctta ctgcttggaa
      661 gaacccgtgg agattcagtg acccagatgg aagggccagt gactctctca gaagaggcct
      721 tcctgactat aaactgcacg tacacagcca caggataccc ttcccttttc tggtatgtcc
      781 aatatcctgg agaaggtcta cagctcctcc tgaaagccac gaaggctgat gacaagggaa
      841 gcaacaaagg ttttgaagcc acataccgta aggaaaccac ttctttccac ttggagaaag
      901 gctcagttca agtgtcagac tcagcggtgt acttctgtgc tctgagtgat aactatggtc
      961 agaattttgt ctttggtccc ggaaccagat tgtccgtgct gccctatatc cagaaccctg
     1021 accctgccgt gtaccagctg agagactcta aatccagtga caagtctgtc tgcctattca
     1081 ccgattttga ttctcaaaca aatgtgtcac aaagtaagga ttctgatgtg tatatcacag
     1141 acaaatgtgt gctagacatg aggtctatgg attttaaatc taattcagca gtagcctgga
     1201 gcaacaaatc tgactttgca tgtgcaaacg ccttcaacaa cagcattatt ccagaagaca
     1261 ccttcttccc cagcccagaa agttcctgtg atgtcaagct ggtcgagaaa agctttgaaa
     1321 cagatacgaa cctaaacttt caaaacctgt cagtgattgg gttccgaatc ctcctcctga
     1381 aagtggccgg gtttaatctg ctcatgacgc tgcggctgtg gtccagctag aaagtggttt
     1441 agtaatgagc tagcgctaac cggtggcgcg ttaagtcgac aatcaacctc tggattacaa
     1501 aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata
     1561 cgctgcttta atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc
     1621 cttgtataaa tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg
     1681 tggcgtggtg tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac
     1741 ctgtcagctc ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat
     1801 cgccgcctgc cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt
     1861 ggtgttgtcg gggaaatcat cgtcctttcc ttggctgctc gcctgtgttg ccacctggat
     1921 tctgcgcggg acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc
     1981 ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag
     2041 tcggatctcc ctttgggccg cctccccgcg tcgactttaa gaccaatgac ttacaaggca
     2101 gctgtagatc ttagccactt tttaaaagaa aaggggggac tggaagggct aattcactcc
     2161 caacgaagac aagatctgct ttttgcttgt actgggtctc tctggttaga ccagatctga
     2221 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
     2281 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
     2341 agaccctttt agtcagtgtg gaaaatctct agcagtacgt atagtagttc atgtcatctt
     2401 attattcagt atttataact tgcaaagaaa tgaatatcag agagtgagag gaacttgttt
     2461 attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca
     2521 tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc
     2581 tggctctagc tatcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg
     2641 cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct
     2701 cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta gggacgtacc
     2761 caattcgccc tatagtgagt cgtattacgc gcgctcactg gccgtcgttt tacaacgtcg
     2821 tgactgggaa aaccctggcg ttacccaact taatcgcctt gcagcacatc cccctttcgc
     2881 cagctggcgt aatagcgaag aggcccgcac cgatcgccct tcccaacagt tgcgcagcct
     2941 gaatggcgaa tgggacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac
     3001 gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc
     3061 ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt
     3121 agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg
     3181 ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac
     3241 gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta
     3301 ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat
     3361 ttaacaaaaa tttaacgcga attttaacaa aatattaacg cttacaattt aggtggcact
     3421 tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg
     3481 tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt
     3541 atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct
     3601 gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca
     3661 cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc
     3721 gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc
     3781 cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg
     3841 gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta
     3901 tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc
     3961 ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt
     4021 gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg
     4081 cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct
     4141 tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc
     4201 tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct
     4261 cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac
     4321 acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc
     4381 tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat
     4441 ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg
     4501 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc
     4561 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
     4621 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
     4681 gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta
     4741 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
     4801 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
     4861 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
     4921 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg
     4981 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
     5041 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
     5101 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
     5161 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
     5221 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct
     5281 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa
     5341 gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg
     5401 cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagttag
     5461 ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgga
     5521 attgtgagcg gataacaatt tcacacagga aacagctatg accatgatta cgccaagcgc
     5581 gcaattaacc ctcactaaag ggaacaaaag ctggagctgc aagcttaatg tagtcttatg
     5641 caatactctt gtagtcttgc aacatggtaa cgatgagtta gcaacatgcc ttacaaggag
     5701 agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg gtacgatcgt gccttattag
     5761 gaaggcaaca gacgggtctg acatggattg gacgaaccac tgaattgccg cattgcagag
     5821 atattgtatt taagtgccta gctcgataca taaacgggtc tctctggtta gaccagatct
     5881 gagcctggga gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc
     5941 cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc
     6001 tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg cgcccgaaca gggacttgaa
     6061 agcgaaaggg aaaccagagg agctctctcg acgcaggact cggcttgctg aagcgcgcac
     6121 ggcaagaggc gaggggcggc gactggtgag tacgccaaaa attttgacta gcggaggcta
     6181 gaaggagaga gatgggtgcg agagcgtcag tattaagcgg gggagaatta gatcgcgatg
     6241 ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat aaattaaaac atatagtatg
     6301 ggcaagcagg gagctagaac gattcgcagt taatcctggc ctgttagaaa catcagaagg
     6361 ctgtagacaa atactgggac agctacaacc atcccttcag acaggatcag aagaacttag
     6421 atcattatat aatacagtag caaccctcta ttgtgtgcat caaaggatag agataaaaga
     6481 caccaaggaa gctttagaca agatagagga agagcaaaac aaaagtaaga ccaccgcaca
     6541 gcaagcggcc gctgatcttc agacctggag gaggagatat gagggacaat tggagaagtg
     6601 aattatataa atataaagta gtaaaaattg aaccattagg agtagcaccc accaaggcaa
     6661 agagaagagt ggtgcagaga gaaaaaagag cagtgggaat aggagctttg ttccttgggt
     6721 tcttgggagc agcaggaagc actatgggcg cagcgtcaat gacgctgacg gtacaggcca
     6781 gacaattatt gtctggtata gtgcagcagc agaacaattt gctgagggct attgaggcgc
     6841 aacagcatct gttgcaactc acagtctggg gcatcaagca gctccaggca agaatcctgg
     6901 ctgtggaaag atacctaaag gatcaacagc tcctggggat ttggggttgc tctggaaaac
     6961 tcatttgcac cactgctgtg ccttggaatg ctagttggag taataaatct ctggaacaga
     7021 tttggaatca cacgacctgg atggagtggg acagagaaat taacaattac acaagcttaa
     7081 tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa gaattattgg
     7141 aattagataa atgggcaagt ttgtggaatt ggtttaacat aacaaattgg ctgtggtata
     7201 taaaattatt cataatgata gtaggaggct tggtaggttt aagaatagtt tttgctgtac
     7261 tttctatagt gaatagagtt aggcagggat attcaccatt atcgtttcag acccacctcc
     7321 caaccccgag gggacccttg cgccttttcc aaggcagccc tgggtttgcg cagggacgcg
     7381 gctgctctgg gcgtggttcc gggaaacgca gcggcgccga ccctgggtct cgcacattct
     7441 tcacgtccgt tcgcagcgtc acccggatct tcgccgctac ccttgtgggc cccccggcga
     7501 cgcttcctgc tccgccccta agtcgggaag gttccttgcg gttcgcggcg tgccggacgt
     7561 gacaaacgga agccgcacgt ctcactagta ccctcgcaga cggacagcgc cagggagcaa
     7621 tggcagcgcg ccgaccgcga tgggctgtgg ccaatagcgg ctgctcagca gggcgcgccg
     7681 agagcagcgg ccgggaaggg gcggtgcggg aggcggggtg tggggcggta gtgtgggccc
     7741 tgttcctgcc cgcgcggtgt tccgcattct gcaagcctcc ggagcgcacg tcggcagtcg
     7801 gctccctcgt tgaccgaatc accgacctct ctccccaggg ggtaccaccg gagcttacca
     7861 tgaccgagta caagcccacg gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac
     7921 gcaccctcgc cgccgcgttc gccgactacc ccgccacgcg ccacaccgtc gatccggacc
     7981 gccacatcga gcgggtcacc gagctgcaag aactcttcct cacgcgcgtc gggctcgaca
     8041 tcggcaaggt gtgggtcgcg gacgacggcg ccgccgtggc ggtctggacc acgccggaga
     8101 gcgtcgaagc gggggcggtg ttcgccgaga tcggcccgcg catggccgag ttgagcggtt
     8161 cccggctggc cgcgcagcaa cagatggaag gcctcctggc gccgcaccgg cccaaggagc
     8221 ccgcgtggtt cctggccacc gtcggcgtct cgcccgacca ccagggcaag ggtctgggca
     8281 gcgccgtcgt gctccccgga gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg
     8341 agacctccgc gccccgcaac ctccccttct acgagcggct cggcttcacc gtcaccgccg
     8401 acgtcgaggt gcccgaagga ccgcgcacct ggtgcatgac ccgcaagccc ggtgcctgag
     8461 aattctagat cttgagacaa atggcagtat tcatccacaa ttttaaaaga aaagggggga
     8521 ttggggggta cagtgcaggg gaaagaatag tagacataat agcaacagac atacaaacta
     8581 aagaattaca aaaacaaatt acaaaaattc aaaattttcg ggtttattac agggacagca
     8641 gagatccact ttggcgccgg ctcgaggggg
//
