LOCUS       sequence-32802-e         864 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  2nd generation lentiviral vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 864)
  TITLE     Tet-On Advanced vectors
REFERENCE   2  (bases 1 to 864)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pLenti CMVtight eGFP Puro (w771-1)
FEATURES             Location/Qualifiers
     source          1..864
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      19..28
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    40..92
                     /label=MCS
                     /note="multiple cloning site"
     protein_bind    complement(121..145)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        complement(223..537)
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     primer_bind     complement(226..250)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    297..315
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    333..351
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    368..386
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    404..422
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    440..458
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    475..493
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    511..529
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     misc_feature    559..676
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 gctcctcgcc cttgctcacc atggtggcga ccggccggtg gatcccgggc ccgcggtacc
       61 gtcgactgca gaattcgaag cttgagctcg agatccagtc gactgaattg gttcctttaa
      121 agcctgcttt tttgtacaaa cttgttgata tctgcagaat tccaccacac tggactagtc
      181 gacgaaccgg tggatccccg ggtaccgagc tcgaattctc caggcgatct gacggttcac
      241 taaacgagct ctgcttatat aggcctccca ccgtacacgc ctacctcgac atacgttctc
      301 tatcactgat agggagtaaa ctcgacatac gttctctatc actgataggg ataaactcga
      361 catacgttct ctatcactga tagggagtaa actcgacata cgttctctat cactgatagg
      421 gagtaaactc gacatacgtt ctctatcact gatagggagt aaactcgaca tcgttctcta
      481 tcactgatag ggagtaaact cgacatacgt tctctatcac tgatagggag taaactcgag
      541 gctagtctcg tgatcgataa aattttgaat ttttgtaatt tgtttttgta attctttagt
      601 ttgtatgtct gttgctatta tgtctactat tctttcccct gcactgtacc ccccaatccc
      661 cccttttctt ttaaaagtta accgataccg tcgagatccg ttcactaatc gaatggatct
      721 gtctctgtct ctctctccac cttcttcttc tattccttcg ggcctgtcgg gtcccctcgg
      781 ggttgggagg tgggtctgaa acgataatgg tgaatatccc tgcctaactc tattcactat
      841 agaaagtaca gcaaaaacta ttct
//
