LOCUS       sequence-328022-        7577 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses FLAG-tagged SFPQ protein in pcDNA3.1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7577)
  AUTHORS   Fukuda Y, Pazyra-Murphy MF, Silagi ES, Tasdemir-Yilmaz OE, Li Y, 
            Rose L, Yeoh ZC, Vangos NE, Geffken EA, Seo HS, Adelmant G, Bird GH,
            Walensky LD, Marto JA, Dhe-Paganon S, Segal RA
  TITLE     Binding and transport of SFPQ-RNA granules by KIF5A/KLC1 motors 
            promotes axon survival.
  JOURNAL   J Cell Biol. 2021 Jan 4;220(1). pii: 211577. doi: 
            10.1083/jcb.202005051.
  PUBMED    33284322
REFERENCE   2  (bases 1 to 7577)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol."; date: "2021-01-4"; volume: "220(1). pii: 211577. doi"; 
            pages: " 10.1083/jcb.202005051"
COMMENT     Sequence Label: FLAG-SFPQ
FEATURES             Location/Qualifiers
     source          1..7577
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(2111..2128)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    2117..2341
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      2387..2815
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2474..2493)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2684..2705
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(2824..2844)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        2829..3158
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      3009..3144
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     3071..3090
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             3225..4019
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3279..3298)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     3889..3908
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    4193..4314
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4230..4249)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4284..4303
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(4363..4379)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4363..4379)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4376..4398)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4387..4403
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4411..4417,4418..4435,4436..4441))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4456..4477
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(4594..4611)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4765..5353)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4845..4864)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5524..6315,6316..6384))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6147..6166
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6385..6489)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(6564..6583)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        6755..7134
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        7135..7338
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     7289..7309
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     7383..7402
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        7383..7401
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             7462..7485
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 ccacgacttc cgttctccgc cgcccggcat gggcctcaat cagaatcgcg gccccatggg
       61 tcctggcccg ggccagagcg gccctaagcc tccgatcccg ccaccgcctc cacaccaaca
      121 gcagcaacag ccaccaccgc agcagccacc gccgcagcag ccgccaccgc atcagccgcc
      181 gccgcatcca cagccgcatc agcagcagca gccgccgcca ccgccgcagg actcttccaa
      241 gcccgtcgtt gctcagggac ccggccccgc tcccggagta ggcagcgcac caccagcctc
      301 cagctcggcc ccgcccgcca ctccaccaac ctcgggggcc ccgccagggt ccgggccagg
      361 cccgactccg accccgccgc ctgcagtcac ctcggcccct cccggggcgc cgccacccac
      421 cccgccaagc agcggggtcc ctaccacacc tcctcaggcc ggaggcccgc cgcctccgcc
      481 cgcggcagtc ccgggcccgg gtccagggcc taagcagggc ccaggtccgg gtggtcccaa
      541 aggcggcaaa atgcctggcg ggccgaagcc aggtggcggc ccgggcctaa gtacgcctgg
      601 cggccacccc aagccgccgc atcgaggcgg cggggagccc cgcgggggcc gccagcacca
      661 cccgccctac caccagcagc atcaccaggg gcccccgccc ggcgggcccg gcggccgcag
      721 cgaggagaag atctcggact cggaggggtt taaagccaat ttgtctctct tgaggaggcc
      781 tggagagaaa acttacacac agcgatgtcg gttgtttgtt gggaatctac ctgctgatat
      841 cacggaggat gagttcaaaa gactatttgc taaatatgga gaaccaggag aagtttttat
      901 caacaaaggc aaaggattcg gatttattaa gcttgaatct agagctttgg ctgaaattgc
      961 caaagccgaa ctggatgata cacccatgag aggtagacag cttcgagttc gctttgccac
     1021 acatgctgct gccctttctg ttcgtaatct ttcaccttat gtttccaatg aactgttgga
     1081 agaagccttt agccaatttg gtcctattga aagggctgtt gtaatagtgg atgatcgtgg
     1141 aagatctaca gggaaaggca ttgttgaatt tgcttctaag ccagcagcaa gaaaggcatt
     1201 tgaacgatgc agtgaaggtg ttttcttact gacgacaact cctcgtccag tcattgtgga
     1261 accacttgaa caactagatg atgaagatgg tcttcctgaa aaacttgccc agaagaatcc
     1321 aatgtatcaa aaggagagag aaacccctcc tcgttttgcc cagcatggca cgtttgagta
     1381 cgaatattct cagcgatgga agtctttgga tgaaatggaa aaacagcaaa gggaacaagt
     1441 tgaaaaaaac atgaaagatg caaaagacaa attggaaagt gaaatggaag atgcctatca
     1501 tgaacatcag gcaaatcttt tgcgccaaga tctgatgaga cgacaggaag aattaagacg
     1561 catggaagaa cttcacaatc aagaaatgca gaaacgtaaa gaaatgcaat tgaggcaaga
     1621 ggaggaacga cgtagaagag aggaagagat gatgattcgt caacgtgaga tggaagaaca
     1681 aatgaggcgc caaagagagg aaagttacag ccgaatgggc tacatggatc cacgggaaag
     1741 agacatgcga atgggtggcg gaggagcaat gaacatggga gatccctatg gttcaggagg
     1801 ccagaaattt ccacctctag gaggtggtgg tggcataggt tatgaagcta atcctggcgt
     1861 tccaccagca accatgagtg gttccatgat gggaagtgac atgcgtactg agcgctttgg
     1921 gcagggaggt gcggggcctg tgggtggaca gggtcctaga ggaatggggc ctggaactcc
     1981 agcaggatat ggtagaggga gagaagagta cgaaggccca aacaaaaaac cccgatttta
     2041 gaattctgca gatatccagc acagtggcgg ccgctcgagt ctagagggcc cgtttaaacc
     2101 cgctgatcag cctcgactgt gccttctagt tgccagccat ctgttgtttg cccctccccc
     2161 gtgccttcct tgaccctgga aggtgccact cccactgtcc tttcctaata aaatgaggaa
     2221 attgcatcgc attgtctgag taggtgtcat tctattctgg ggggtggggt ggggcaggac
     2281 agcaaggggg aggattggga agacaatagc aggcatgctg gggatgcggt gggctctatg
     2341 gcttctgagg cggaaagaac cagctggggc tctagggggt atccccacgc gccctgtagc
     2401 ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc
     2461 gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt
     2521 ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac
     2581 ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag
     2641 acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa
     2701 actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg
     2761 atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattaattc
     2821 tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg ctccccagca ggcagaagta
     2881 tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag
     2941 caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc ccgcccctaa
     3001 ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc catggctgac
     3061 taattttttt tatttatgca gaggccgagg ccgcctctgc ctctgagcta ttccagaagt
     3121 agtgaggagg cttttttgga ggcctaggct tttgcaaaaa gctcccggga gcttgtatat
     3181 ccattttcgg atctgatcaa gagacaggat gaggatcgtt tcgcatgatt gaacaagatg
     3241 gattgcacgc aggttctccg gccgcttggg tggagaggct attcggctat gactgggcac
     3301 aacagacaat cggctgctct gatgccgccg tgttccggct gtcagcgcag gggcgcccgg
     3361 ttctttttgt caagaccgac ctgtccggtg ccctgaatga actgcaggac gaggcagcgc
     3421 ggctatcgtg gctggccacg acgggcgttc cttgcgcagc tgtgctcgac gttgtcactg
     3481 aagcgggaag ggactggctg ctattgggcg aagtgccggg gcaggatctc ctgtcatctc
     3541 accttgctcc tgccgagaaa gtatccatca tggctgatgc aatgcggcgg ctgcatacgc
     3601 ttgatccggc tacctgccca ttcgaccacc aagcgaaaca tcgcatcgag cgagcacgta
     3661 ctcggatgga agccggtctt gtcgatcagg atgatctgga cgaagagcat caggggctcg
     3721 cgccagccga actgttcgcc aggctcaagg cgcgcatgcc cgacggcgag gatctcgtcg
     3781 tgacccatgg cgatgcctgc ttgccgaata tcatggtgga aaatggccgc ttttctggat
     3841 tcatcgactg tggccggctg ggtgtggcgg accgctatca ggacatagcg ttggctaccc
     3901 gtgatattgc tgaagagctt ggcggcgaat gggctgaccg cttcctcgtg ctttacggta
     3961 tcgccgctcc cgattcgcag cgcatcgcct tctatcgcct tcttgacgag ttcttctgag
     4021 cgggactctg gggttcgaaa tgaccgacca agcgacgccc aacctgccat cacgagattt
     4081 cgattccacc gccgccttct atgaaaggtt gggcttcgga atcgttttcc gggacgccgg
     4141 ctggatgatc ctccagcgcg gggatctcat gctggagttc ttcgcccacc ccaacttgtt
     4201 tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc
     4261 atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt
     4321 ctgtataccg tcgacctcta gctagagctt ggcgtaatca tggtcatagc tgtttcctgt
     4381 gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa
     4441 agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc
     4501 tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag
     4561 aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt
     4621 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga
     4681 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg
     4741 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa
     4801 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
     4861 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
     4921 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct
     4981 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
     5041 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
     5101 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc
     5161 tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat
     5221 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa
     5281 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa
     5341 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga
     5401 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct
     5461 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga
     5521 cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc
     5581 catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg
     5641 ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat
     5701 aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat
     5761 ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg
     5821 caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc
     5881 attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa
     5941 agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc
     6001 actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt
     6061 ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag
     6121 ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt
     6181 gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag
     6241 atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac
     6301 cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc
     6361 gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca
     6421 gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg
     6481 ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc gacggatcgg gagatctccc
     6541 gatcccctat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat
     6601 ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg cgagcaaaat ttaagctaca
     6661 acaaggcaag gcttgaccga caattgcatg aagaatctgc ttagggttag gcgttttgcg
     6721 ctgcttcgcg atgtacgggc cagatatacg cgttgacatt gattattgac tagttattaa
     6781 tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg cgttacataa
     6841 cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata
     6901 atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag
     6961 tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc
     7021 cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta
     7081 tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg
     7141 cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt
     7201 ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca
     7261 aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag
     7321 gtctatataa gcagagctct ctggctaact agagaaccca ctgcttactg gcttatcgaa
     7381 attaatacga ctcactatag ggagacccaa gctggctagc gtttaaactt aagcttggta
     7441 ccgagctcgg atcccaccat ggactacaaa gacgatgacg acaagggatc cggaatgtct
     7501 cgggatcggt tccggagtcg tggcggtggc ggtggtggct tccacaggcg tggaggaggc
     7561 ggcggccgcg gcggcct
//
