LOCUS       sequence-328026-       10472 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral plasmid expressing Halo-tagged SFPQ protein from the EF1a
            promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10472)
  AUTHORS   Fukuda Y, Pazyra-Murphy MF, Silagi ES, Tasdemir-Yilmaz OE, Li Y, 
            Rose L, Yeoh ZC, Vangos NE, Geffken EA, Seo HS, Adelmant G, Bird GH,
            Walensky LD, Marto JA, Dhe-Paganon S, Segal RA
  TITLE     Binding and transport of SFPQ-RNA granules by KIF5A/KLC1 motors 
            promotes axon survival.
  JOURNAL   J Cell Biol. 2021 Jan 4;220(1). pii: 211577. doi: 
            10.1083/jcb.202005051.
  PUBMED    33284322
REFERENCE   2  (bases 1 to 10472)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol."; date: "2021-01-4"; volume: "220(1). pii: 211577. doi"; 
            pages: " 10.1083/jcb.202005051"
COMMENT     Sequence Label: pLV-EF1a-HaloSFPQ
FEATURES             Location/Qualifiers
     source          1..10472
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    47..172
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    669..902
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1087..1131
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1394..1509
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     promoter        1587..1798
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     intron          1799..2737
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     2694..2714
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      2752..2761
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2758..3645
                     /codon_start=1
                     /product="modified bacterial dehalogenase that forms 
                     covalent bonds with chloroalkane derivatives"
                     /label=HaloTag(R)
                     /translation="MEIGTGFPFDPHYVEVLGERMHYVDVGPRDGTPVLFLHGNPTSSY
                     VWRNIIPHVAPTHRCIAPDLIGMGKSDKPDLGYFFDDHVRFMDAFIEALGLEEVVLVIH
                     DWGSALGFHWAKRNPERVKGIAFMEFIRPIPTWDEWPEFARETFQAFRTTDVGRKLIID
                     QNVFIEGTLPMGVVRPLTEVEMDHYREPFLNPVDREPLWRFPNELPIAGEPANIVALVE
                     EYMDWLHQSPVPKLLFWGTPGVLIPPAEAARLAKSLPNCKAVDIGPGLNLLQEDNPDLI
                     GSEIARWLSTLEISG"
     misc_feature    5807..6395
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5860..5880)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6278..6289)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6602..7235
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(7363..7379)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7363..7379)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7376..7398)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7387..7403
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7411..7417,7418..7435,7436..7441))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7456..7477
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7594..7611)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7765..8353)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7845..7864)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8524..9315,9316..9384))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9147..9166
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9385..9489)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    9537..9671
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9574..9593)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9628..9647
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             9839..10472
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
ORIGIN
        1 gtggcgcccg aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag
       61 gactcggctt gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc
      121 aaaaattttg actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa
      181 gcgggggaga attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa
      241 atataaatta aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc
      301 tggcctgtta gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct
      361 tcagacagga tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt
      421 gcatcaaagg atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca
      481 aaacaaaagt aagaccaccg cacagcaagc ggccggccgc tgatcttcag acctggagga
      541 ggagatatga gggacaattg gagaagtgaa ttatataaat ataaagtagt aaaaattgaa
      601 ccattaggag tagcacccac caaggcaaag agaagagtgg tgcagagaga aaaaagagca
      661 gtgggaatag gagctttgtt ccttgggttc ttgggagcag caggaagcac tatgggcgca
      721 gcgtcaatga cgctgacggt acaggccaga caattattgt ctggtatagt gcagcagcag
      781 aacaatttgc tgagggctat tgaggcgcaa cagcatctgt tgcaactcac agtctggggc
      841 atcaagcagc tccaggcaag aatcctggct gtggaaagat acctaaagga tcaacagctc
      901 ctggggattt ggggttgctc tggaaaactc atttgcacca ctgctgtgcc ttggaatgct
      961 agttggagta ataaatctct ggaacagatt tggaatcaca cgacctggat ggagtgggac
     1021 agagaaatta acaattacac aagcttaata cactccttaa ttgaagaatc gcaaaaccag
     1081 caagaaaaga atgaacaaga attattggaa ttagataaat gggcaagttt gtggaattgg
     1141 tttaacataa caaattggct gtggtatata aaattattca taatgatagt aggaggcttg
     1201 gtaggtttaa gaatagtttt tgctgtactt tctatagtga atagagttag gcagggatat
     1261 tcaccattat cgtttcagac ccacctccca accccgaggg gacccgacag gcccgaagga
     1321 atagaagaag aaggtggaga gagagacaga gacagatcca ttcgattagt gaacggatct
     1381 cgacggtatc gcctttaaaa gaaaaggggg gattgggggg tacagtgcag gggaaagaat
     1441 agtagacata atagcaacag acatacaaac taaagaatta caaaaacaaa ttacaaaaat
     1501 tcaaaatttt cgggtttatt acagggacag cagagatcca gtttatcgat gaggcccttt
     1561 cgtcttcact cgaggtgccc gtcagtgggc agagcgcaca tcgcccacag tccccgagaa
     1621 gttgggggga ggggtcggca attgaaccgg tgcctagaga aggtggcgcg gggtaaactg
     1681 ggaaagtgat gtcgtgtact ggctccgcct ttttcccgag ggtgggggag aaccgtatat
     1741 aagtgcagta gtcgccgtga acgttctttt tcgcaacggg tttgccgcca gaacacaggt
     1801 aagtgccgtg tgtggttccc gcgggcctgg cctctttacg ggttatggcc cttgcgtgcc
     1861 ttgaattact tccacctggc tgcagtacgt gattcttgat cccgagcttc gggttggaag
     1921 tgggtgggag agttcgaggc cttgcgctta aggagcccct tcgcctcgtg cttgagttga
     1981 ggcctggcct gggcgctggg gccgccgcgt gcgaatctgg tggcaccttc gcgcctgtct
     2041 cgctgctttc gataagtctc tagccattta aaatttttga tgacctgctg cgacgctttt
     2101 tttctggcaa gatagtcttg taaatgcggg ccaagatctg cacactggta tttcggtttt
     2161 tggggccgcg ggcggcgacg gggcccgtgc gtcccagcgc acatgttcgg cgaggcgggg
     2221 cctgcgagcg cggccaccga gaatcggacg ggggtagtct caagctggcc ggcctgctct
     2281 ggtgcctggc ctcgcgccgc cgtgtatcgc cccgccctgg gcggcaaggc tggcccggtc
     2341 ggcaccagtt gcgtgagcgg aaagatggcc gcttcccggc cctgctgcag ggagctcaaa
     2401 atggaggacg cggcgctcgg gagagcgggc gggtgagtca cccacacaaa ggaaaagggc
     2461 ctttccgtcc tcagccgtcg cttcatgtga ctccacggag taccgggcgc cgtccaggca
     2521 cctcgattag ttctcgagct tttggagtac gtcgtcttta ggttgggggg aggggtttta
     2581 tgcgatggag tttccccaca ctgagtgggt ggagactgaa gttaggccag cttggcactt
     2641 gatgtaattc tccttggaat ttgccctttt tgagtttgga tcttggttca ttctcaagcc
     2701 tcagacagtg gttcaaagtt tttttcttcc atttcaggtg tcgtgaggat cgccaccatg
     2761 gaaatcggta ctggctttcc attcgacccc cattatgtgg aagtcctggg cgagcgcatg
     2821 cactacgtcg atgttggtcc gcgcgatggc acccctgtgc tgttcctgca cggtaacccg
     2881 acctcctcct acgtgtggcg caacatcatc ccgcatgttg caccgaccca tcgctgcatt
     2941 gctccagacc tgatcggtat gggcaaatcc gacaaaccag acctgggtta tttcttcgac
     3001 gaccacgtcc gcttcatgga tgccttcatc gaagccctgg gtctggaaga ggtcgtcctg
     3061 gtcattcacg actggggctc cgctctgggt ttccactggg ccaagcgcaa tccagagcgc
     3121 gtcaaaggta ttgcatttat ggagttcatc cgccctatcc cgacctggga cgaatggcca
     3181 gaatttgccc gcgagacctt ccaggccttc cgcaccaccg acgtcggccg caagctgatc
     3241 atcgatcaga acgtttttat cgagggtacg ctgccgatgg gtgtcgtccg cccgctgact
     3301 gaagtcgaga tggaccatta ccgcgagccg ttcctgaatc ctgttgaccg cgagccactg
     3361 tggcgcttcc caaacgagct gccaatcgcc ggtgagccag cgaacatcgt cgcgctggtc
     3421 gaagaataca tggactggct gcaccagtcc cctgtcccga agctgctgtt ctggggcacc
     3481 ccaggcgttc tgatcccacc ggccgaagcc gctcgcctgg ccaaaagcct gcctaactgc
     3541 aaggctgtgg acatcggccc gggtctgaat ctgctgcaag aagacaaccc ggacctgatc
     3601 ggcagcgaga tcgcgcgctg gctgtctact ctggagattt ccggtggcgc cccgtacaag
     3661 atgtctcggg atcggttccg gagtcgtggc ggtggcggtg gtggcttcca caggcgtgga
     3721 ggaggcggcg gccgcggcgg cctccacgac ttccgttctc cgccgcccgg catgggcctc
     3781 aatcagaatc gcggccccat gggtcctggc ccgggccaga gcggccctaa gcctccgatc
     3841 ccgccaccgc ctccacacca acagcagcaa cagccaccac cgcagcagcc accgccgcag
     3901 cagccgccac cgcatcagcc gccgccgcat ccacagccgc atcagcagca gcagccgccg
     3961 ccaccgccgc aggactcttc caagcccgtc gttgctcagg gacccggccc cgctcccgga
     4021 gtaggcagcg caccaccagc ctccagctcg gccccgcccg ccactccacc aacctcgggg
     4081 gccccgccag ggtccgggcc aggcccgact ccgaccccgc cgcctgcagt cacctcggcc
     4141 cctcccgggg cgccgccacc caccccgcca agcagcgggg tccctaccac acctcctcag
     4201 gccggaggcc cgccgcctcc gcccgcggca gtcccgggcc cgggtccagg gcctaagcag
     4261 ggcccaggtc cgggtggtcc caaaggcggc aaaatgcctg gcgggccgaa gccaggtggc
     4321 ggcccgggcc taagtacgcc tggcggccac cccaagccgc cgcatcgagg cggcggggag
     4381 ccccgcgggg gccgccagca ccacccgccc taccaccagc agcatcacca ggggcccccg
     4441 cccggcgggc ccggcggccg cagcgaggag aagatctcgg actcggaggg gtttaaagcc
     4501 aatttgtctc tcttgaggag gcctggagag aaaacttaca cacagcgatg tcggttgttt
     4561 gttgggaatc tacctgctga tatcacggag gatgagttca aaagactatt tgctaaatat
     4621 ggagaaccag gagaagtttt tatcaacaaa ggcaaaggat tcggatttat taagcttgaa
     4681 tctagagctt tggctgaaat tgccaaagcc gaactggatg atacacccat gagaggtaga
     4741 cagcttcgag ttcgctttgc cacacatgct gctgcccttt ctgttcgtaa tctttcacct
     4801 tatgtttcca atgaactgtt ggaagaagcc tttagccaat ttggtcctat tgaaagggct
     4861 gttgtaatag tggatgatcg tggaagatct acagggaaag gcattgttga atttgcttct
     4921 aagccagcag caagaaaggc atttgaacga tgcagtgaag gtgttttctt actgacgaca
     4981 actcctcgtc cagtcattgt ggaaccactt gaacaactag atgatgaaga tggtcttcct
     5041 gaaaaacttg cccagaagaa tccaatgtat caaaaggaga gagaaacccc tcctcgtttt
     5101 gcccagcatg gcacgtttga gtacgaatat tctcagcgat ggaagtcttt ggatgaaatg
     5161 gaaaaacagc aaagggaaca agttgaaaaa aacatgaaag atgcaaaaga caaattggaa
     5221 agtgaaatgg aagatgccta tcatgaacat caggcaaatc ttttgcgcca agatctgatg
     5281 agacgacagg aagaattaag acgcatggaa gaacttcaca atcaagaaat gcagaaacgt
     5341 aaagaaatgc aattgaggca agaggaggaa cgacgtagaa gagaggaaga gatgatgatt
     5401 cgtcaacgtg agatggaaga acaaatgagg cgccaaagag aggaaagtta cagccgaatg
     5461 ggctacatgg atccacggga aagagacatg cgaatgggtg gcggaggagc aatgaacatg
     5521 ggagatccct atggttcagg aggccagaaa tttccacctc taggaggtgg tggtggcata
     5581 ggttatgaag ctaatcctgg cgttccacca gcaaccatga gtggttccat gatgggaagt
     5641 gacatgcgta ctgagcgctt tgggcaggga ggtgcggggc ctgtgggtgg acagggtcct
     5701 agaggaatgg ggcctggaac tccagcagga tatggtagag ggagagaaga gtacgaaggc
     5761 ccaaacaaaa aaccccgatt ttagccgggc ggggcgcgtc tggaacaatc aacctctgga
     5821 ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg
     5881 tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt
     5941 ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag
     6001 gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc
     6061 caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga
     6121 actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa
     6181 ttccgtggtg ttgtcgggga agctgacgtc ctttccatgg ctgctcgcct gtgttgccac
     6241 ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct
     6301 tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca
     6361 gacgagtcgg atctcccttt gggccgcctc cccgcctgga attaattctg cagtcgagac
     6421 ctagaaaaac atggagcaat cacaagtagc aatacagcag ctaccaatgc tgattgtgcc
     6481 tggctagaag cacaagagga ggaggaggtg ggttttccag tcacacctca ggtaccttta
     6541 agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga aaagagggga
     6601 ctggaagggc taattcactc ccaacgaaga caagatatcc ttgatctgtg gatctaccac
     6661 acacaaggct acttccctga ttagcagaac tacacaccag ggccaggggt cagatatcca
     6721 ctgacctttg gatggtgcta caagctagta ccagttgagc cagataaggt agaagaggcc
     6781 aataaaggag agaacaccag cttgttacac cctgtgagcc tgcatgggat ggatgacccg
     6841 gagagagaag tgttagagtg gaggtttgac agccgcctag catttcatca cgtggcccga
     6901 gagctgcatc cggagtactt caagaactgc tgatatcgag cttgctacaa gggactttcc
     6961 gctggggact ttccagggag gcgtggcctg ggcgggactg gggagtggcg agccctcaga
     7021 tcctgcatat aagcagctgc tttttgcctg tactgggtct ctctggttag accagatctg
     7081 agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc
     7141 ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct
     7201 cagacccttt tagtcagtgt ggaaaatctc tagcagtagt agttcatgtc atcttattat
     7261 tcagtattta taacttgcaa agaaatgaat atcagagagt gagaggcctt gacattgcta
     7321 gcgtttaccg tcgacctcta gctagagctt ggcgtaatca tggtcatagc tgtttcctgt
     7381 gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa
     7441 agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc
     7501 tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag
     7561 aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt
     7621 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga
     7681 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg
     7741 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa
     7801 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
     7861 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
     7921 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct
     7981 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
     8041 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
     8101 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc
     8161 tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat
     8221 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa
     8281 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa
     8341 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga
     8401 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct
     8461 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga
     8521 cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc
     8581 catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg
     8641 ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat
     8701 aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat
     8761 ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg
     8821 caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc
     8881 attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa
     8941 agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc
     9001 actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt
     9061 ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag
     9121 ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt
     9181 gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag
     9241 atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac
     9301 cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc
     9361 gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca
     9421 gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg
     9481 ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc gacggatcgg gagatcaact
     9541 tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata
     9601 aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc
     9661 atgtctggat caactggata actcaagcta accaaaatca tcccaaactt cccaccccat
     9721 accctattac cactgccaat tacctgtggt ttcatttact ctaaacctgt gattcctctg
     9781 aattattttc attttaaaga aattgtattt gttaaatatg tactacaaac ttagtagttg
     9841 gaagggctaa ttcactccca aagaagacaa gatatccttg atctgtggat ctaccacaca
     9901 caaggctact tccctgatta gcagaactac acaccagggc caggggtcag atatccactg
     9961 acctttggat ggtgctacaa gctagtacca gttgagccag ataaggtaga agaggccaat
    10021 aaaggagaga acaccagctt gttacaccct gtgagcctgc atgggatgga tgacccggag
    10081 agagaagtgt tagagtggag gtttgacagc cgcctagcat ttcatcacgt ggcccgagag
    10141 ctgcatccgg agtacttcaa gaactgctga tatcgagctt gctacaaggg actttccgct
    10201 ggggactttc cagggaggcg tggcctgggc gggactgggg agtggcgagc cctcagatcc
    10261 tgcatataag cagctgcttt ttgcctgtac tgggtctctc tggttagacc agatctgagc
    10321 ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg
    10381 agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag
    10441 acccttttag tcagtgtgga aaatctctag ca
//
