LOCUS       sequence-328027-       12288 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral plasmid expressing Halo-tagged SFPQ protein from the UbC 
            promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12288)
  AUTHORS   Fukuda Y, Pazyra-Murphy MF, Silagi ES, Tasdemir-Yilmaz OE, Li Y, 
            Rose L, Yeoh ZC, Vangos NE, Geffken EA, Seo HS, Adelmant G, Bird GH,
            Walensky LD, Marto JA, Dhe-Paganon S, Segal RA
  TITLE     Binding and transport of SFPQ-RNA granules by KIF5A/KLC1 motors 
            promotes axon survival.
  JOURNAL   J Cell Biol. 2021 Jan 4;220(1). pii: 211577. doi: 
            10.1083/jcb.202005051.
  PUBMED    33284322
REFERENCE   2  (bases 1 to 12288)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol."; date: "2021-01-4"; volume: "220(1). pii: 211577. doi"; 
            pages: " 10.1083/jcb.202005051"
COMMENT     Sequence Label: UbC-Halo-SFPQ
FEATURES             Location/Qualifiers
     source          1..12288
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        358..556
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     511..531
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             574..754
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    801..926
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1419..1652
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1837..1881
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2179..2296
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2361..3572
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     3409..3428
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     regulatory      3618..3627
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3624..4511
                     /codon_start=1
                     /product="modified bacterial dehalogenase that forms 
                     covalent bonds with chloroalkane derivatives"
                     /label=HaloTag(R)
                     /translation="MEIGTGFPFDPHYVEVLGERMHYVDVGPRDGTPVLFLHGNPTSSY
                     VWRNIIPHVAPTHRCIAPDLIGMGKSDKPDLGYFFDDHVRFMDAFIEALGLEEVVLVIH
                     DWGSALGFHWAKRNPERVKGIAFMEFIRPIPTWDEWPEFARETFQAFRTTDVGRKLIID
                     QNVFIEGTLPMGVVRPLTEVEMDHYREPFLNPVDREPLWRFPNELPIAGEPANIVALVE
                     EYMDWLHQSPVPKLLFWGTPGVLIPPAEAARLAKSLPNCKAVDIGPGLNLLQEDNPDLI
                     GSEIARWLSTLEISG"
     misc_feature    6719..7307
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6772..6792)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7190..7201)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(7310..7326)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7311..7327)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             7832..8012
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(8038..8055)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8044..8268
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      8314..8742
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8401..8420)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8611..8632
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(8751..8771)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        8756..9085
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8936..9071
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     8998..9017
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        9133..9180
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             9199..9573
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    9703..9824
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9740..9759)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9794..9813
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(9873..9889)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9873..9889)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9886..9908)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9897..9913
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9921..9927,9928..9945,9946..9951))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9966..9987
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(10104..10121)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10275..10863)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10355..10374)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(11034..11825,11826..11894))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11657..11676
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11895..11999)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(12074..12093)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        join(12265..12288,1..356)
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 aatagtaatc aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat
       61 aacttacggt aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa
      121 taatgacgta tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg
      181 agtatttacg gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc
      241 cccctattga cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct
      301 tatgggactt tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga
      361 tgcggttttg gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa
      421 gtctccaccc cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc
      481 caaaatgtcg taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg
      541 aggtctatat aagcagcgcg ttttgcctgt actgggtctc tctggttaga ccagatctga
      601 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      661 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      721 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      781 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      841 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      901 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      961 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
     1021 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
     1081 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1141 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1201 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1261 aagcggccgc tgatcttcag acctggagga ggagatatga gggacaattg gagaagtgaa
     1321 ttatataaat ataaagtagt aaaaattgaa ccattaggag tagcacccac caaggcaaag
     1381 agaagagtgg tgcagagaga aaaaagagca gtgggaatag gagctttgtt ccttgggttc
     1441 ttgggagcag caggaagcac tatgggcgca gcgtcaatga cgctgacggt acaggccaga
     1501 caattattgt ctggtatagt gcagcagcag aacaatttgc tgagggctat tgaggcgcaa
     1561 cagcatctgt tgcaactcac agtctggggc atcaagcagc tccaggcaag aatcctggct
     1621 gtggaaagat acctaaagga tcaacagctc ctggggattt ggggttgctc tggaaaactc
     1681 atttgcacca ctgctgtgcc ttggaatgct agttggagta ataaatctct ggaacagatt
     1741 tggaatcaca cgacctggat ggagtgggac agagaaatta acaattacac aagcttaata
     1801 cactccttaa ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga attattggaa
     1861 ttagataaat gggcaagttt gtggaattgg tttaacataa caaattggct gtggtatata
     1921 aaattattca taatgatagt aggaggcttg gtaggtttaa gaatagtttt tgctgtactt
     1981 tctatagtga atagagttag gcagggatat tcaccattat cgtttcagac ccacctccca
     2041 accccgaggg gacccgacag gcccgaagga atagaagaag aaggtggaga gagagacaga
     2101 gacagatcca ttcgattagt gaacggatcg gcactgcgtg cgccaattct gcagacaaat
     2161 ggcagtattc atccacaatt ttaaaagaaa aggggggatt ggggggtaca gtgcagggga
     2221 aagaatagta gacataatag caacagacat acaaactaaa gaattacaaa aacaaattac
     2281 aaaaattcaa aattttcggg tttattacag ggacagcaga gatccagttt ggttaattaa
     2341 cccgtgtcgg ctccagatct ggcctccgcg ccgggttttg gcgcctcccg cgggcgcccc
     2401 cctcctcacg gcgagcgctg ccacgtcaga cgaagggcgc agcgagcgtc ctgatccttc
     2461 cgcccggacg ctcaggacag cggcccgctg ctcataagac tcggccttag aaccccagta
     2521 tcagcagaag gacattttag gacgggactt gggtgactct agggcactgg ttttctttcc
     2581 agagagcgga acaggcgagg aaaagtagtc ccttctcggc gattctgcgg agggatctcc
     2641 gtggggcggt gaacgccgat gattatataa ggacgcgccg ggtgtggcac agctagttcc
     2701 gtcgcagccg ggatttgggt cgcggttctt gtttgtggat cgctgtgatc gtcacttggt
     2761 gagtagcggg ctgctgggct ggccggggct ttcgtggccg ccgggccgct cggtgggacg
     2821 gaagcgtgtg gagagaccgc caagggctgt agtctgggtc cgcgagcaag gttgccctga
     2881 actgggggtt ggggggagcg cagcaaaatg gcggctgttc ccgagtcttg aatggaagac
     2941 gcttgtgagg cgggctgtga ggtcgttgaa acaaggtggg gggcatggtg ggcggcaaga
     3001 acccaaggtc ttgaggcctt cgctaatgcg ggaaagctct tattcgggtg agatgggctg
     3061 gggcaccatc tggggaccct gacgtgaagt ttgtcactga ctggagaact cggtttgtcg
     3121 tctgttgcgg gggcggcagt tatggcggtg ccgttgggca gtgcacccgt acctttggga
     3181 gcgcgcgccc tcgtcgtgtc gtgacgtcac ccgttctgtt ggcttataat gcagggtggg
     3241 gccacctgcc ggtaggtgtg cggtaggctt ttctccgtcg caggacgcag ggttcgggcc
     3301 tagggtaggc tctcctgaat cgacaggcgc cggacctctg gtgaggggag ggataagtga
     3361 ggcgtcagtt tctttggtcg gttttatgta cctatcttct taagtagctg aagctccggt
     3421 tttgaactat gcgctcgggg ttggcgagtg tgttttgtga agttttttag gcaccttttg
     3481 aaatgtaatc atttgggtca atatgtaatt ttcagtgtta gactagtaaa ttgtccgcta
     3541 aattctggcc gtttttggct tttttgttag acgaagcttg ggctgcaggt cgactctaga
     3601 ggatccccgg gtaccgggcc accatggaaa tcggtactgg ctttccattc gacccccatt
     3661 atgtggaagt cctgggcgag cgcatgcact acgtcgatgt tggtccgcgc gatggcaccc
     3721 ctgtgctgtt cctgcacggt aacccgacct cctcctacgt gtggcgcaac atcatcccgc
     3781 atgttgcacc gacccatcgc tgcattgctc cagacctgat cggtatgggc aaatccgaca
     3841 aaccagacct gggttatttc ttcgacgacc acgtccgctt catggatgcc ttcatcgaag
     3901 ccctgggtct ggaagaggtc gtcctggtca ttcacgactg gggctccgct ctgggtttcc
     3961 actgggccaa gcgcaatcca gagcgcgtca aaggtattgc atttatggag ttcatccgcc
     4021 ctatcccgac ctgggacgaa tggccagaat ttgcccgcga gaccttccag gccttccgca
     4081 ccaccgacgt cggccgcaag ctgatcatcg atcagaacgt ttttatcgag ggtacgctgc
     4141 cgatgggtgt cgtccgcccg ctgactgaag tcgagatgga ccattaccgc gagccgttcc
     4201 tgaatcctgt tgaccgcgag ccactgtggc gcttcccaaa cgagctgcca atcgccggtg
     4261 agccagcgaa catcgtcgcg ctggtcgaag aatacatgga ctggctgcac cagtcccctg
     4321 tcccgaagct gctgttctgg ggcaccccag gcgttctgat cccaccggcc gaagccgctc
     4381 gcctggccaa aagcctgcct aactgcaagg ctgtggacat cggcccgggt ctgaatctgc
     4441 tgcaagaaga caacccggac ctgatcggca gcgagatcgc gcgctggctg tctactctgg
     4501 agatttccgg tggcgccccg tacaagatgt ctcgggatcg gttccggagt cgtggcggtg
     4561 gcggtggtgg cttccacagg cgtggaggag gcggcggccg cggcggcctc cacgacttcc
     4621 gttctccgcc gcccggcatg ggcctcaatc agaatcgcgg ccccatgggt cctggcccgg
     4681 gccagagcgg ccctaagcct ccgatcccgc caccgcctcc acaccaacag cagcaacagc
     4741 caccaccgca gcagccaccg ccgcagcagc cgccaccgca tcagccgccg ccgcatccac
     4801 agccgcatca gcagcagcag ccgccgccac cgccgcagga ctcttccaag cccgtcgttg
     4861 ctcagggacc cggccccgct cccggagtag gcagcgcacc accagcctcc agctcggccc
     4921 cgcccgccac tccaccaacc tcgggggccc cgccagggtc cgggccaggc ccgactccga
     4981 ccccgccgcc tgcagtcacc tcggcccctc ccggggcgcc gccacccacc ccgccaagca
     5041 gcggggtccc taccacacct cctcaggccg gaggcccgcc gcctccgccc gcggcagtcc
     5101 cgggcccggg tccagggcct aagcagggcc caggtccggg tggtcccaaa ggcggcaaaa
     5161 tgcctggcgg gccgaagcca ggtggcggcc cgggcctaag tacgcctggc ggccacccca
     5221 agccgccgca tcgaggcggc ggggagcccc gcgggggccg ccagcaccac ccgccctacc
     5281 accagcagca tcaccagggg cccccgcccg gcgggcccgg cggccgcagc gaggagaaga
     5341 tctcggactc ggaggggttt aaagccaatt tgtctctctt gaggaggcct ggagagaaaa
     5401 cttacacaca gcgatgtcgg ttgtttgttg ggaatctacc tgctgatatc acggaggatg
     5461 agttcaaaag actatttgct aaatatggag aaccaggaga agtttttatc aacaaaggca
     5521 aaggattcgg atttattaag cttgaatcta gagctttggc tgaaattgcc aaagccgaac
     5581 tggatgatac acccatgaga ggtagacagc ttcgagttcg ctttgccaca catgctgctg
     5641 ccctttctgt tcgtaatctt tcaccttatg tttccaatga actgttggaa gaagccttta
     5701 gccaatttgg tcctattgaa agggctgttg taatagtgga tgatcgtgga agatctacag
     5761 ggaaaggcat tgttgaattt gcttctaagc cagcagcaag aaaggcattt gaacgatgca
     5821 gtgaaggtgt tttcttactg acgacaactc ctcgtccagt cattgtggaa ccacttgaac
     5881 aactagatga tgaagatggt cttcctgaaa aacttgccca gaagaatcca atgtatcaaa
     5941 aggagagaga aacccctcct cgttttgccc agcatggcac gtttgagtac gaatattctc
     6001 agcgatggaa gtctttggat gaaatggaaa aacagcaaag ggaacaagtt gaaaaaaaca
     6061 tgaaagatgc aaaagacaaa ttggaaagtg aaatggaaga tgcctatcat gaacatcagg
     6121 caaatctttt gcgccaagat ctgatgagac gacaggaaga attaagacgc atggaagaac
     6181 ttcacaatca agaaatgcag aaacgtaaag aaatgcaatt gaggcaagag gaggaacgac
     6241 gtagaagaga ggaagagatg atgattcgtc aacgtgagat ggaagaacaa atgaggcgcc
     6301 aaagagagga aagttacagc cgaatgggct acatggatcc acgggaaaga gacatgcgaa
     6361 tgggtggcgg aggagcaatg aacatgggag atccctatgg ttcaggaggc cagaaatttc
     6421 cacctctagg aggtggtggt ggcataggtt atgaagctaa tcctggcgtt ccaccagcaa
     6481 ccatgagtgg ttccatgatg ggaagtgaca tgcgtactga gcgctttggg cagggaggtg
     6541 cggggcctgt gggtggacag ggtcctagag gaatggggcc tggaactcca gcaggatatg
     6601 gtagagggag agaagagtac gaaggcccaa acaaaaaacc ccgattttag ggcgcgcccc
     6661 tgcaggcacc acactggact agtgctagcg ttaacaattc gatatcaagc ttatcgataa
     6721 tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc
     6781 ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat
     6841 ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg
     6901 gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg
     6961 ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat
     7021 tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt
     7081 gggcactgac aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt ggctgctcgc
     7141 ctgtgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa
     7201 tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg
     7261 ccttcgccct cagacgagtc ggatctccct ttgggccgcc tccccgcatc gataccgtcg
     7321 acctcgagac ctagaaaaac atggagcaat cacaagtagc aatacagcag ctaccaatgc
     7381 tgattgtgcc tggctagaag cacaagagga ggaggaggtg ggttttccag tcacacctca
     7441 ggtaccttta agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga
     7501 aaagggggga ctggaagggc taattcactc ccaacgaaga caagatatcc ttgatctgtg
     7561 gatctaccac acacaaggct acttccctga ttggcagaac tacacaccag ggccagggat
     7621 cagatatcca ctgacctttg gatggtgcta caagctagta ccagttgagc aagagaaggt
     7681 agaagaagcc aatgaaggag agaacacccg cttgttacac cctgtgagcc tgcatgggat
     7741 ggatgacccg gagagagaag tattagagtg gaggtttgac agccgcctag catttcatca
     7801 catggcccga gagctgcatc cggactgtac tgggtctctc tggttagacc agatctgagc
     7861 ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg
     7921 agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag
     7981 acccttttag tcagtgtgga aaatctctag cagggcccgt ttaaacccgc tgatcagcct
     8041 cgactgtgcc ttctagttgc cagccatctg ttgtttgccc ctcccccgtg ccttccttga
     8101 ccctggaagg tgccactccc actgtccttt cctaataaaa tgaggaaatt gcatcgcatt
     8161 gtctgagtag gtgtcattct attctggggg gtggggtggg gcaggacagc aagggggagg
     8221 attgggaaga caatagcagg catgctgggg atgcggtggg ctctatggct tctgaggcgg
     8281 aaagaaccag ctggggctct agggggtatc cccacgcgcc ctgtagcggc gcattaagcg
     8341 cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg
     8401 ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc
     8461 taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa
     8521 aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc
     8581 ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac
     8641 tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt
     8701 ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttaattctgt ggaatgtgtg
     8761 tcagttaggg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca
     8821 tctcaattag tcagcaacca ggtgtggaaa gtccccaggc tccccagcag gcagaagtat
     8881 gcaaagcatg catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc
     8941 gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat
     9001 ttatgcagag gccgaggccg cctctgcctc tgagctattc cagaagtagt gaggaggctt
     9061 ttttggaggc ctaggctttt gcaaaaagct cccgggagct tgtatatcca ttttcggatc
     9121 tgatcagcac gtgttgacaa ttaatcatcg gcatagtata tcggcatagt ataatacgac
     9181 aaggtgagga actaaaccat ggccaagttg accagtgccg ttccggtgct caccgcgcgc
     9241 gacgtcgccg gagcggtcga gttctggacc gaccggctcg ggttctcccg ggacttcgtg
     9301 gaggacgact tcgccggtgt ggtccgggac gacgtgaccc tgttcatcag cgcggtccag
     9361 gaccaggtgg tgccggacaa caccctggcc tgggtgtggg tgcgcggcct ggacgagctg
     9421 tacgccgagt ggtcggaggt cgtgtccacg aacttccggg acgcctccgg gccggccatg
     9481 accgagatcg gcgagcagcc gtgggggcgg gagttcgccc tgcgcgaccc ggccggcaac
     9541 tgcgtgcact tcgtggccga ggagcaggac tgacacgtgc tacgagattt cgattccacc
     9601 gccgccttct atgaaaggtt gggcttcgga atcgttttcc gggacgccgg ctggatgatc
     9661 ctccagcgcg gggatctcat gctggagttc ttcgcccacc ccaacttgtt tattgcagct
     9721 tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc atttttttca
     9781 ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctgtataccg
     9841 tcgacctcta gctagagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt
     9901 tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt
     9961 gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg
    10021 ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg
    10081 cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg
    10141 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat
    10201 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc
    10261 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc
    10321 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
    10381 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
    10441 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg
    10501 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
    10561 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg
    10621 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc
    10681 ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg
    10741 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc
    10801 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct
    10861 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt
    10921 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa
    10981 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa
    11041 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc
    11101 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct
    11161 gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca
    11221 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt
    11281 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt
    11341 gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc
    11401 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc
    11461 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt
    11521 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact
    11581 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc
    11641 ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt
    11701 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg
    11761 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct
    11821 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa
    11881 tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt
    11941 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc
    12001 acatttcccc gaaaagtgcc acctgacgtc gacggatcgg gagatctccc gatcccctat
    12061 ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat ctgctccctg
    12121 cttgtgtgtt ggaggtcgct gagtagtgcg cgagcaaaat ttaagctaca acaaggcaag
    12181 gcttgaccga caattgcatg aagaatctgc ttagggttag gcgttttgcg ctgcttcgcg
    12241 atgtacgggc cagatatacg cgttgacatt gattattgac tagttatt
//
